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1. 发明授权

US09058169B2 Method and system for waking up remote devices 有权
标题翻译：唤醒远程设备的方法和系统
公开(公告)号：US09058169B2
公开(公告)日：2015-06-16
申请号：US13882410
申请日：2011-09-08
申请人： Xiangning Chen , Jie Zhang , Lifang Hao
发明人： Xiangning Chen , Jie Zhang , Lifang Hao
IPC分类号： G06F1/32 , H04M1/738 , H04W52/02
CPC分类号： G06F1/3234 , H04M1/738 , H04W52/0229 , Y02D70/00
摘要： Disclosed are a method and a system for remote wake-up and status check, with a wake-up signal generating unit generating and transmitting a wake-up signal to wake up an electricity supply unit of a remote device to operate normally and provide electricity for the remote device. The wake-up signal has an assigned electric voltage with specific characteristics for detection by the electricity supply unit while in power-off mode and, upon signal detection, the electricity supply unit wakes up to provide normal electric voltage output. The wake-up signal generating unit determines, according to an electric current on a wake-up signal feed line, whether the remote device is in an active mode. A low wake-up energy consumption enables system wake-up and a true powered-off sleep mode.
摘要翻译：公开了一种用于远程唤醒和状态检查的方法和系统，其中唤醒信号产生单元产生并发送唤醒信号以唤醒远程设备的电力供应单元以正常运行并为远程设备。唤醒信号具有分配电压，具有特定的特性，用于在断电模式下由电力供应单元进行检测，并且在信号检测时，供电单元唤醒以提供正常的电压输出。唤醒信号发生单元根据唤醒信号馈送线上的电流来确定远程设备是否处于活动模式。低唤醒能耗使系统唤醒和真正关闭睡眠模式。

2. 发明申请

US20130214759A1 LONG-DISTANCE CONSTANT-VOLTAGE ELECTRICITY-FEEDING METHOD WITH WAKE-UP FUNCTION AND SYSTEM 审中-公开
标题翻译：具有唤醒功能和系统的长距离恒电压馈电方式
公开(公告)号：US20130214759A1
公开(公告)日：2013-08-22
申请号：US13882137
申请日：2011-09-08
申请人： Xiangning Chen , Lifang Hao , Jie Zhang
发明人： Xiangning Chen , Lifang Hao , Jie Zhang
IPC分类号： H02J4/00
CPC分类号： H02J4/00 , H04L12/10 , H04L12/12 , H04L12/40039 , H04L12/40045 , Y02D50/20 , Y02D50/40
摘要： Disclosed are a wired long-distance constant-voltage electricity-feeding method with a wake-up function and a system. A smart electricity supply module of a central electricity supply device generates a feed voltage from a central electricity source, and feeds the voltage to a terminal electricity source module through a feed line. Said smart electricity supply module can continuously provide electricity at a constant voltage to the terminal electricity source module and can change feed voltage polarity according to set rules when the terminal electricity module in sleep-mode must be remotely waken up. A voltage polarity monitoring module of said terminal electricity source module can determine, by monitoring the polarity of the voltage of the centrally fed electricity, whether to wake up the terminal electricity source module from sleep-mode to enter a normal electricity-supplying mode. The electrical feed circuit and the wake-up function are easy to implement, and provide a versatile power feed and high energy efficiency while reducing withstand-voltage process requirements.
摘要翻译：公开了具有唤醒功能和系统的有线长距离恒压供电方法。中央供电装置的智能电力供应模块从中央电源产生馈电电压，并通过馈电线将电压馈送到终端电源模块。所述智能电力供应模块可以连续向终端电源模块提供恒定电压，并且当休眠模式下的终端电力模块必须远程唤醒时，可以根据设定规则改变馈电电压极性。所述终端电源模块的电压极性监视模块可以通过监视中央馈电电压的极性来确定终端电源模块是否从休眠模式唤醒，进入正常供电模式。供电电路和唤醒功能易于实现，并提供通用的馈电和高能效，同时降低耐压工艺要求。

3. 发明申请

US20130219204A1 METHOD AND SYSTEM FOR WAKING UP REMOTE DEVICES 有权
标题翻译：用于调出远程设备的方法和系统
公开(公告)号：US20130219204A1
公开(公告)日：2013-08-22
申请号：US13882410
申请日：2011-09-08
申请人： Xiangning Chen , Jie Zhang , Lifang Hao
发明人： Xiangning Chen , Jie Zhang , Lifang Hao
IPC分类号： G06F1/32
CPC分类号： G06F1/3234 , H04M1/738 , H04W52/0229 , Y02D70/00
摘要： Disclosed are a method and a system for remote wake-up and status check, with a wake-up signal generating unit generating and transmitting a wake-up signal to wake up an electricity supply unit of a remote device to operate normally and provide electricity for the remote device. The wake-up signal has an assigned electric voltage with specific characteristics for detection by the electricity supply unit while in power-off mode and, upon signal detection, the electricity supply unit wakes up to provide normal electric voltage output. The wake-up signal generating unit determines, according to an electric current on a wake-up signal feed line, whether the remote device is in an active mode. A low wake-up energy consumption enables system wake-up and a true powered-off sleep mode.
摘要翻译：公开了一种用于远程唤醒和状态检查的方法和系统，其中唤醒信号产生单元产生并发送唤醒信号以唤醒远程设备的电力供应单元以正常运行并为远程设备。唤醒信号具有分配电压，具有特定的特性，用于在断电模式下由电力供应单元进行检测，并且在信号检测时，供电单元唤醒以提供正常的电压输出。唤醒信号发生单元根据唤醒信号馈送线上的电流来确定远程设备是否处于活动模式。低唤醒能耗使系统唤醒和真正关闭睡眠模式。

4. 发明授权

US07262030B2 Multiple sequencible and ligatible structures for genomic analysis 失效
标题翻译：用于基因组分析的多个可连续和相容的结构
公开(公告)号：US07262030B2
公开(公告)日：2007-08-28
申请号：US10476970
申请日：2002-05-09
申请人： Xiangning Chen
发明人： Xiangning Chen
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6853 , C12Q1/6806 , C12Q1/6858 , C12Q2535/125 , C12Q2549/119 , C12Q2525/161 , C12Q2521/319 , C12Q2535/131 , C12Q2525/204
摘要： High throughput methods and kits for single nucleotide polymorphism (SNP) genotyping are provided. The methods involve utilizing nested PCR amplification reactions which produce sequencible and ligatible structures. An outer PCR primer set amplifies the SNP, and an inner PCR primer set amplifies a portion of the DNA amplified by the outer primer set, but does not amplify the SNP itself. The inner and outer primers may reaction include non-target common domain sequences, and the inner primer common domain sequences may comprise digestion restriction endonuclease recognition sites. The design of the inner primer set allows precise tailoring of the sequencible and ligatible structures with respect to length and base composition.
摘要翻译：提供了用于单核苷酸多态性（SNP）基因分型的高通量方法和试剂盒。该方法涉及利用嵌套PCR扩增反应，其产生可连续和可连接的结构。外部PCR引物组扩增SNP，内部PCR引物组扩增由外部引物组扩增的DNA的一部分，但不扩增SNP本身。内部和外部引物可以反应包括非目标共同结构域序列，并且内部引物共同结构域序列可以包含消化限制性内切核酸酶识别位点。内部引物组的设计允许相对于长度和基础组成精确定制可序列和可连接的结构。

5. 发明授权

US06177249B1 Method for nucleic acid analysis using fluorescence resonance energy transfer 有权
标题翻译：使用荧光共振能量转移进行核酸分析的方法
公开(公告)号：US06177249B1
公开(公告)日：2001-01-23
申请号：US09295026
申请日：1999-04-20
申请人： Pui-Yan Kwok , Xiangning Chen
发明人： Pui-Yan Kwok , Xiangning Chen
IPC分类号： C12Q168
CPC分类号： C12Q1/6818 , C12Q2600/156 , C12Q2565/1015 , C12Q2561/125 , C12Q2535/125 , C12Q2525/186 , C12Q2563/107
摘要： A method for detecting the presence of a target nucleotide or sequence of nucleotides in a nucleic acid is disclosed. The method is comprised of forming an oligonucleotide labeled with two fluorophores on the nucleic acid target site. The doubly labeled oligonucleotide is formed by addition of a singly labeled dideoxynucleoside triphosphate to a singly labeled polynucleotide or by ligation of two singly labeled polynucleotides. Detection of fluorescence resonance energy transfer upon denaturation indicates the presence of the target. Kits are also provided. The method is particularly applicable to genotyping.
摘要翻译：公开了一种用于检测核酸中靶核苷酸或核苷酸序列的存在的方法。该方法包括在核酸靶位点上形成用两个荧光团标记的寡核苷酸。通过将单标记的双脱氧核苷三磷酸加成到单标记的多核苷酸中或通过连接两个单标记的多核苷酸来形成双重标记的寡核苷酸。在变性时检测荧光共振能量转移指示靶的存在。还提供套件。该方法特别适用于基因分型。

6. 发明授权

US5945283A Methods and kits for nucleic acid analysis using fluorescence resonance energy transfer 失效
标题翻译：使用荧光共振能量转移进行核酸分析的方法和试剂盒
公开(公告)号：US5945283A
公开(公告)日：1999-08-31
申请号：US767979
申请日：1996-12-17
申请人： Pui-Yan Kwok , Xiangning Chen
发明人： Pui-Yan Kwok , Xiangning Chen
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： C12Q1/6818 , C12Q2600/156
摘要： A method for detecting the presence of a target nucleotide or sequence of nucleotides in a nucleic acid is disclosed. The method is comprised of forming an oligonucleotide labeled with two fluorophores on the nucleic acid target site. The doubly labeled oligonucleotide is formed by addition of a singly labeled dideoxynucleoside triphosphate to a singly labeled polynucleotide or by ligation of two singly labeled polynucleotides. Detection of fluorescence resonance energy transfer upon denaturation indicates the presence of the target. Kits are also provided. The method is particularly applicable to genotyping.
摘要翻译：公开了一种用于检测核酸中靶核苷酸或核苷酸序列的存在的方法。该方法包括在核酸靶位点上形成用两个荧光团标记的寡核苷酸。通过将单标记的双脱氧核苷三磷酸加成到单标记的多核苷酸中或通过连接两个单标记的多核苷酸来形成双重标记的寡核苷酸。在变性时检测荧光共振能量转移指示靶的存在。还提供套件。该方法特别适用于基因分型。

7. 发明授权

US06440707B1 Fluorescence polarization in nucleic acid analysis 失效
标题翻译：核酸分析中的荧光极化
公开(公告)号：US06440707B1
公开(公告)日：2002-08-27
申请号：US09708346
申请日：2000-11-07
申请人： Pui-Yan Kwok , Xiangning Chen , Leanna Levine
发明人： Pui-Yan Kwok , Xiangning Chen , Leanna Levine
IPC分类号： C12P1934
CPC分类号： C12Q1/6816 , C12Q1/6827 , C12Q2527/125 , C12Q2522/101 , C12Q2563/107 , C12Q2561/119 , C12Q2535/131
摘要： A new method for DNA diagnostics based on template-directed primer extension and detection by fluorescence polarization is described. In this method, amplified genomic DNA fragments containing polymorphic sites are incubated with a oligonucleotide primer designed to hybridize to the DNA template adjacent to the polymorphic site in the presence of allelic dye-labeled dideoxyribonucleoside triphosphates and a modified Taq DNA polymerase. The primer is extended by the dye-terminator specific for the allele present on the template. At the end of the reaction, the fluorescence polarization of the two dye-terminators in the reaction mixture are analyzed directly without separation or purification. This homogeneous DNA diagnostic method is shown to be highly sensitive and specific and is suitable for automated genotyping of large number or samples.
摘要翻译：描述了基于模板定向引物延伸和通过荧光偏振检测的DNA诊断的新方法。在该方法中，将含有多态性位点的扩增的基因组DNA片段与设计为在等位基因染料标记的二脱氧核糖核苷三磷酸和修饰的Taq DNA聚合酶存在下与多态性位点相邻的DNA模板杂交的寡核苷酸引物温育。该引物由对模板上存在的等位基因特异性的染料终止子延长。在反应结束时，直接分析反应混合物中两个染料 - 终体化合物的荧光偏振而不进行分离或纯化。这种均一的DNA诊断方法显示出高度敏感和特异性，适用于大量或样品的自动基因分型。

8. 发明申请

US20050214840A1 Restriction enzyme mediated method of multiplex genotyping 审中-公开
标题翻译：限制酶介导的多重基因分型方法
公开(公告)号：US20050214840A1
公开(公告)日：2005-09-29
申请号：US11086401
申请日：2005-03-23
申请人： Xiangning Chen
发明人： Xiangning Chen
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6876 , C12Q2525/161 , C12Q2521/313
摘要： A method for single nucleotide polymorphism (SNP) genotyping using widely available DNA sequencers is provided. A restriction endonuclease recognition site is incorporated into a PCR primer for the SNP, a restriction enzyme is used to cleave the DNA and create extendable ends at target polymorphic sites, and an extension reaction is used to create allele-specific extension products that can be distinguished using DNA sequencers or other detection platforms.
摘要翻译：提供了使用广泛可用的DNA测序仪进行单核苷酸多态性（SNP）基因分型的方法。将限制性内切核酸酶识别位点并入SNP的PCR引物中，使用限制酶切割DNA并在靶多态性位点处产生可扩展末端，并且使用延伸反应来产生可区分的等位基因特异性扩增产物使用DNA测序仪或其他检测平台。

9. 发明申请

US20050175996A1 Multiple sequencible and ligatible structures for genomic analysis 失效
标题翻译：用于基因组分析的多个可连续和相容的结构
公开(公告)号：US20050175996A1
公开(公告)日：2005-08-11
申请号：US10476970
申请日：2002-05-09
申请人： Xiangning Chen
发明人： Xiangning Chen
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6853 , C12Q1/6806 , C12Q1/6858 , C12Q2535/125 , C12Q2549/119 , C12Q2525/161 , C12Q2521/319 , C12Q2535/131 , C12Q2525/204
摘要： High throughput methods and kits for single nucleotide polymorphism (SNP) genotyping are provided. The methods involve utilizing nested PCR amplification reactions which produce sequencible and ligatible structures. An outer PCR primer set amplifies the SNP, and an inner PCR primer set amplifies a portion of the DNA amplified by the outer primer set, but does not amplify the SNP itself. The inner and outer primers may reaction include non-target common domain sequences, and the inner primer common domain sequences may comprise digestion restriction endonuclease recognition sites. The design of the inner primer set allows precise tailoring of the sequencible and ligatible structures with respect to length and base composition.
摘要翻译：提供了用于单核苷酸多态性（SNP）基因分型的高通量方法和试剂盒。该方法涉及利用嵌套PCR扩增反应，其产生可连续和可连接的结构。外部PCR引物组扩增SNP，内部PCR引物组扩增由外部引物组扩增的DNA的一部分，但不扩增SNP本身。内部和外部引物可以反应包括非目标共同结构域序列，并且内部引物共同结构域序列可以包含消化限制性内切核酸酶识别位点。内部引物组的设计允许相对于长度和基础组成精确定制可序列和可连接的结构。

10. 发明授权

US06180408B2 Fluorescence polarization in nucleic acid analysis 有权
标题翻译：核酸分析中的荧光极化
公开(公告)号：US06180408B2
公开(公告)日：2001-01-30
申请号：US09137826
申请日：1998-08-21
申请人： Pui-Yan Kwok , Xiangning Chen , Leanna Levine
发明人： Pui-Yan Kwok , Xiangning Chen , Leanna Levine
IPC分类号： C12Q168
CPC分类号： C12Q1/6816 , C12Q1/6827 , C12Q2527/125 , C12Q2522/101 , C12Q2563/107 , C12Q2561/119 , C12Q2535/131
摘要： A new method for DNA diagnostics based on template-directed primer extension and detection by fluorescence polarization is described. In this method, amplified genomic DNA fragments containing polymorphic sites are incubated with a oligonucleotide primer designed to hybridize to the DNA template adjacent to the polymorphic site in the presence of allelic dye-labeled dideoxyribonucleoside triphosphates and a modified Taq DNA polymerase. The primer is extended by the dye-terminator specific for the allele present on the template. At the end of the reaction, the fluorescence polarization of the two dye-terminators in the reaction mixture are analyzed directly without separation or purification. This homogeneous DNA diagnostic method is shown to be highly sensitive and specific and is suitable for automated genotyping of large number or samples.
摘要翻译：描述了基于模板定向引物延伸和通过荧光偏振检测的DNA诊断的新方法。在该方法中，将含有多态性位点的扩增的基因组DNA片段与设计为在等位基因染料标记的二脱氧核糖核苷三磷酸和修饰的Taq DNA聚合酶存在下与多态性位点相邻的DNA模板杂交的寡核苷酸引物温育。该引物由对模板上存在的等位基因特异性的染料终止子延长。在反应结束时，直接分析反应混合物中两个染料 - 终体化合物的荧光偏振而不进行分离或纯化。这种均一的DNA诊断方法显示出高度敏感和特异性，适用于大量或样品的自动基因分型。

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