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1. 发明申请

US20110237781A1 METHOD OF PREPARING ALPHA-1 PROTEINASE INHIBITOR 审中-公开
标题翻译：制备ALPHA-1蛋白酶抑制剂的方法
公开(公告)号：US20110237781A1
公开(公告)日：2011-09-29
申请号：US13002429
申请日：2009-07-17
申请人： Wytold Lebing , Scott A. Cook , Christopher A. Dadd
发明人： Wytold Lebing , Scott A. Cook , Christopher A. Dadd
IPC分类号： C07K1/36
CPC分类号： C07K14/8125
摘要： Purification of α-1 proteinase inhibitor (α-1 PI) from solutions comprising α-1 PI is accomplished using hydrophobic interaction chromatography (HIC). In some embodiments, purification of α-1 PI is accomplished by precipitation of contaminating proteins from a starting solution comprising α-1 PI, such as human plasma, followed by anion exchange resin chromatography prior to HIC. Further purification may be accomplished by an optional cation exchange chromatography subsequent to anion exchange chromatography but prior to HIC. Some embodiments of the invention also include virus removal and/or inactivation by methods such as nano filtration and such as contact with a non-ionic detergent. The methods of the present invention result in greater yield, purity, and pathogenic clearance of plasma fractions than known methods.
摘要翻译：使用疏水相互作用色谱（HIC）实现从包含α-1 PI的溶液中纯化α-1蛋白酶抑制剂（α-1 PI）。在一些实施方案中，通过从包含α-1PI的起始溶液（例如人血浆）中沉淀污染性蛋白，然后在HIC之前进行阴离子交换树脂色谱来完成α-1 PI的纯化。进一步纯化可以通过阴离子交换层析之后但在HIC之前的任选的阳离子交换层析来完成。本发明的一些实施方案还包括通过诸如纳米过滤的方法和诸如与非离子型洗涤剂接触的病毒去除和/或灭活。与已知方法相比，本发明的方法产生更高的产量，纯度和血浆级分的致病性清除。

2. 发明授权

US07544500B2 Process for the production of a reversibly inactive acidified plasmin composition 无效
标题翻译：用于生产可逆失活的酸化纤溶酶组合物的方法
公开(公告)号：US07544500B2
公开(公告)日：2009-06-09
申请号：US10692105
申请日：2003-10-23
申请人： Rita T. Bradley , Scott A. Cook , Christopher A. Dadd , Jonathan D. Kent , Marina N. Korneyeva , Valery V. Novokhatny , James F. Rebbeor , Christopher J. Stenland , Jonathan S. Strauss , Jarrett C. Terry , Jeffrey A. Yuziuk
发明人： Rita T. Bradley , Scott A. Cook , Christopher A. Dadd , Jonathan D. Kent , Marina N. Korneyeva , Valery V. Novokhatny , James F. Rebbeor , Christopher J. Stenland , Jonathan S. Strauss , Jarrett C. Terry , Jeffrey A. Yuziuk
IPC分类号： C12N9/50 , C12N9/68 , C12N9/70
CPC分类号： A61K38/4833 , A61K38/484 , A61K47/02 , A61K47/12 , A61K47/183 , A61K47/26 , C12N9/6435 , C12Y304/21007
摘要： Disclosed is both a process for producing a reversibly inactive acidified plasmin by activating plasminogen and a process for producing a purified plasminogen. The produced plasmin is isolated and stored with a low pH-buffering capacity agent to provide a substantially stable formulation. The purified plasminogen is typically purified from a fraction obtained in the separation of immunoglobulin from Fraction II+III chromatographic process and eluted at a low pH. The reversibly inactive acidified plasmin may be used in the administration of a thrombolytic therapy.
摘要翻译：本发明公开了通过活化纤溶酶原产生可逆失活的酸化纤溶酶的方法和用于产生纯化的纤溶酶原的方法。分离产生的纤溶酶并用低pH缓冲能力试剂储存以提供基本上稳定的制剂。纯化的纤溶酶原通常从从级分II + III色谱法分离免疫球蛋白获得的级分中纯化，并在低pH下洗脱。可逆惰性的酸化纤溶酶可以用于溶栓治疗的施用。

3. 发明授权

US5844087A Method and device for delivering fibrin glue 失效
标题翻译：用于输送纤维蛋白胶的方法和装置
公开(公告)号：US5844087A
公开(公告)日：1998-12-01
申请号：US744488
申请日：1996-11-05
申请人： Thomas P. Zimmerman , Christopher A. Dadd , George A. Baumbach
发明人： Thomas P. Zimmerman , Christopher A. Dadd , George A. Baumbach
IPC分类号： A61J1/05 , A61B17/00 , A61B17/03 , A61L24/10 , A61K35/14 , A61M5/00 , B01D63/00
CPC分类号： A61L24/106 , A61B17/00491 , A61B2017/00495
摘要： A method and device for activating and applying a solution of fibrinogen to a desired site. The method includes contacting the solution of fibrinogen with immobilized thrombin resulting in an activated solution of polymerizable fibrin, and delivering the activated solution to the desired site. The device includes a housing having a compartment for a solution of fibrinogen, immobilized thrombin, a structure for bringing the solution of fibrinogen in contact with the immobilized thrombin under conditions which permit the activation of the fibrinogen resulting in polymerizable fibrin, and a structure for delivery of the activated solution to the desired site.
摘要翻译：用于激活和应用纤维蛋白原溶液到所需部位的方法和装置。该方法包括使纤维蛋白原溶液与固定的凝血酶接触，产生活化的可聚合纤维蛋白溶液，并将活化的溶液输送到所需位点。该装置包括具有用于纤维蛋白原溶液，固定化凝血酶的隔室的壳体，用于使纤维蛋白原溶液与固定的凝血酶接触的结构，所述结构在允许纤维蛋白原激活导致可聚合纤维蛋白的条件下，以及用于递送的结构的活化溶液。

4. 发明授权

US5723579A Fibrinogen binding peptides 失效
标题翻译：纤维蛋白原结合肽
公开(公告)号：US5723579A
公开(公告)日：1998-03-03
申请号：US595718
申请日：1996-02-02
申请人： Joseph A. Buettner , Christopher A. Dadd , George A. Baumbach , David J. Hammond
发明人： Joseph A. Buettner , Christopher A. Dadd , George A. Baumbach , David J. Hammond
IPC分类号： C07K1/22 , C07K5/103 , C07K5/107 , C07K7/06 , C07K14/75 , C07K17/00 , A61K38/08 , C07K7/04
CPC分类号： C07K7/06 , C07K5/101 , C07K5/1016
摘要： Peptides which bind to fibrinogen are disclosed. These peptides have available fibrinogen binding domains having a Phe-Leu-Leu-Val or Leu-Leu-Val-Pro sequence. The preferred peptide is a 6-mer having the sequence Phe-Leu-Leu-Val-Pro-Leu (SEQ ID NO:1). A method of using these peptides in an affinity chromatography process to purify fibrinogen is described.
摘要翻译：公开了结合纤维蛋白原的肽。这些肽具有可用的具有Phe-Leu-Leu-Val或Leu-Leu-Val-Pro序列的纤维蛋白原结合结构域。优选的肽是具有序列Phe-Leu-Leu-Val-Pro-Leu（SEQ ID NO：1）的6聚体。描述了在亲和层析方法中使用这些肽以净化纤维蛋白原的方法。

5. 发明授权

US6121422A Method and device for delivering fibrin glue 有权
标题翻译：用于输送纤维蛋白胶的方法和装置
公开(公告)号：US6121422A
公开(公告)日：2000-09-19
申请号：US200636
申请日：1998-11-25
申请人： Thomas P. Zimmerman , Christopher A. Dadd , George A. Baumbach
发明人： Thomas P. Zimmerman , Christopher A. Dadd , George A. Baumbach
IPC分类号： A61J1/05 , A61B17/00 , A61B17/03 , A61L24/10 , A61K35/14 , A61M5/00 , B01D63/00
CPC分类号： A61L24/106 , A61B17/00491 , A61B2017/00495
摘要： A method and device for activating and applying a solution of fibrinogen to form fibrin glue at a desired site. The method includes contacting the solution of fibrinogen with immobilized thrombin under conditions that result in a solution of polymerizable fibrin and delivering the activated solution to the desired site. The device includes a housing having a compartment for a solution of fibrinogen, a container for immobilized thrombin, a structure following the solution of fibrinogen to be brought into contact with the immobilized thrombin under conditions which permit the activation of the fibrinogen to polymerizable fibrin, and a structure allowing for delivery of the activated solution to the desired site.
摘要翻译：用于在期望的位置激活和施加纤维蛋白原溶液以形成纤维蛋白胶的方法和装置。该方法包括在导致可聚合纤维蛋白溶液并将活化溶液输送到所需位点的条件下使纤维蛋白原溶液与固定化凝血酶接触。该装置包括具有用于纤维蛋白原溶液的隔室，用于固定化凝血酶的容器的结构，在允许纤维蛋白原激活到可聚合纤维蛋白的条件下，纤维蛋白原溶液与待固化的凝血酶接触的结构，以及允许将活化溶液输送到所需位置的结构。

6. 发明授权

US5688912A Peptide ligands which bind to von willebrand factor 失效
标题翻译：结合血管性血友病因子的肽配体
公开(公告)号：US5688912A
公开(公告)日：1997-11-18
申请号：US537069
申请日：1995-09-22
申请人： Christopher A. Dadd , George A. Baumbach , David J. Hammond
发明人： Christopher A. Dadd , George A. Baumbach , David J. Hammond
IPC分类号： G01N33/53 , B01J20/281 , C07K1/22 , C07K14/00 , C07K14/475 , C07K14/745 , C12N9/00 , G01N30/88 , G01N33/566 , C07K7/04 , A61K35/14 , C07K17/00
CPC分类号： C07K14/745 , C07K14/001
摘要： Peptide ligands which bind to von Willebrand Factor (vWF) are disclosed. These peptides are selected from the group consisting of RLHSFY, RLKSFY, RLNSFY, RLQSFY, RFRSFY, RIRSFY, RVRSFY, RYRSFY, RLRSFY, HLRSFY, and KLRSFY. The preferred peptide ligand has the sequence RVRSFY. A method of using the disclosed peptides to purify vWF comprises binding the peptides to a support, then passing a solution containing vWF over the supports under condition such that the vWF will selectively bind to the peptides, and then eluting the vWF.
摘要翻译：公开了结合血管性血友病因子（vWFF）的肽配体。这些肽选自RLHSFY，RLKSFY，RLNSFY，RLQSFY，RFRSFY，RIRSFY，RVRSFY，RYRSFY，RLRSFY，HLRSFY和KLRSFY。优选的肽配体具有序列RVRSFY。使用所公开的肽来纯化vWF的方法包括将肽结合到载体上，然后在含有vWF的溶液中使含有vWF的溶液在条件下通过，使得vWF将选择性地结合肽，然后洗脱vWF。

7. 发明授权

US5831003A Peptides which bind to prothrombin and thrombin 失效
标题翻译：结合凝血酶原和凝血酶的肽
公开(公告)号：US5831003A
公开(公告)日：1998-11-03
申请号：US672805
申请日：1996-06-28
申请人： George A. Baumbach , Christopher A. Dadd , Joseph A. Buettner , David J. Hammond
发明人： George A. Baumbach , Christopher A. Dadd , Joseph A. Buettner , David J. Hammond
IPC分类号： C07K1/22 , C07K7/06 , C07K14/745 , C12N9/74 , C07K7/00
CPC分类号： C07K7/06 , C07K14/745 , C12N9/6429 , C12Y304/21005
摘要： Peptide ligands which bind to thrombin and prothrombin are disclosed. The sequences of these peptides are Gln-Leu-Trp-Gly-Ser-His, Arg-Gln-Leu-Trp-Gly-Ser-His, His-Gln-Leu-Trp-Gly-Ser-His, and Tyr-Phe-Pro-Gly-Pro-Tyr-Leu. The preferred peptides have the sequence Gln-Leu-Trp-Gly-Ser-His or Tyr-Phe-Pro-Gly-Pro-Tyr-Leu. A method of using these peptides in an affinity chromatography process to purify thrombin is described.
摘要翻译：公开了结合凝血酶和凝血酶原的肽配体。这些肽的序列是Gln-Leu-Trp-Gly-Ser-His，Arg-Gln-Leu-Trp-Gly-Ser-His，His-Gln-Leu-Trp-Gly-Ser-His和Tyr-Phe -Pro-Gly-Pro-Tyr-Leu。优选的肽具有序列Gln-Leu-Trp-Gly-Ser-His或Tyr-Phe-Pro-Gly-Pro-Tyr-Leu。描述了在亲和层析方法中使用这些肽以净化凝血酶的方法。

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