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1. 发明申请

WO2006113861A3 MAMMALIAN EXPRESSION SYSTEMS 审中-公开
公开(公告)号：WO2006113861A3
公开(公告)日：2006-10-26
申请号：PCT/US2006/014859
申请日：2006-04-20
申请人： WYETH , ZHONG, Xiaotian , KRIZ, Ronald , STAHL, Mark
发明人： ZHONG, Xiaotian , KRIZ, Ronald , STAHL, Mark
IPC分类号： C12N5/06 , C07K14/71 , C08B37/10
摘要： The present invention features mammalian expression systems with improved production yields, and method of using these systems to produce desired proteins. In one embodiment, the expression systems of the present invention comprise genetically-engineered mammalian host cells cultured in a medium that contains an effective amount of heparin or heparin-like molecules. The presence of heparin or heparin-like molecules significantly increases protein production by the cultured cells. The present invention also features the use of constitutively-active components of FGFR-I -mediated signal transduction pathways to improve protein production by cultured mammalian cells. Co-expression of such a component with a protein of interest markedly increases the production yield of the protein of interest.

2. 发明申请

WO2007092370A2 STRUCTURE LINGO-1 审中-公开
标题翻译：结构LINGO-1
公开(公告)号：WO2007092370A2
公开(公告)日：2007-08-16
申请号：PCT/US2007/003015
申请日：2007-02-01
申请人： WYETH , MOSYAK, Lidia , DWYER, Brian , JOHNSON, Mark , ZHONG, Xiaotian , PRESMAN, Eleonora , WILHELM, James M. , STAHL, Mark , SOMERS, William Stuart
发明人： MOSYAK, Lidia , DWYER, Brian , JOHNSON, Mark , ZHONG, Xiaotian , PRESMAN, Eleonora , WILHELM, James M. , STAHL, Mark , SOMERS, William Stuart
CPC分类号： C07K14/4703 , C07K14/705 , C07K2299/00
摘要： Cette invention concerne des polypeptides LINGO-1, des complexes polypeptide/ligand LINGO-1, des cristaux de polypeptides LINGO-1, des cristaux de complexes polypeptide/ligand LINGO-1, ainsi que des méthodes associées et des systèmes logiciels.
摘要翻译： Cette发明协调多肽LINGO-1，des复合物多肽/配体LINGO-1，des cristaux de多肽LINGO-1，des cristaux de complexes多肽/配体LINGO-1，ainsi que desméthodesassociéeset dessystèmeslogiciels。

3. 发明申请

WO2007025248A3 AGGRECANASE STRUCTURE 审中-公开
公开(公告)号：WO2007025248A3
公开(公告)日：2007-03-01
申请号：PCT/US2006/033498
申请日：2006-08-24
申请人： WYETH , MOSYAK, Lidia , RUSH III, Thomas Saltmarsh , ZHONG, Xiaotian , MCDONAGH, Thomas E. , GEORGIADIS, Katy E. , SUM, Phaik-Eng , LAVALLIE, Edward R. , COLLINS-RACIE, Lisa A. , CORCORAN, Christopher John , KUMAR, Ravindra , HEBERT, Tracy , OLLAND, Stephane Hubert , MACKIE, Stewart Andrews
发明人： MOSYAK, Lidia , RUSH III, Thomas Saltmarsh , ZHONG, Xiaotian , MCDONAGH, Thomas E. , GEORGIADIS, Katy E. , SUM, Phaik-Eng , LAVALLIE, Edward R. , COLLINS-RACIE, Lisa A. , CORCORAN, Christopher John , KUMAR, Ravindra , HEBERT, Tracy , OLLAND, Stephane Hubert , MACKIE, Stewart Andrews
IPC分类号： C12N9/64 , G06F19/00 , G01N23/20 , C30B7/00
摘要： This invention relates to aggrecanase polypeptides and aggrecanase polypeptide/ligand complexes, crystals of aggrecanase and aggrecanase polypeptide/ligand complexes, and related methods and software systems.

4. 发明申请

WO2008060307A2 METHODS OF LABELING TRANSIENTLY EXPRESSED PROTEINS IN LARGE-SCALE EUKARYOTIC CELL CULTURES 审中-公开
标题翻译：在大型真核细胞培养基中标记瞬时表达的蛋白质的方法
公开(公告)号：WO2008060307A2
公开(公告)日：2008-05-22
申请号：PCT/US2007/000336
申请日：2007-01-09
申请人： WYETH , ZHONG, Xiaotian , KRIZ, Ronald, William
发明人： ZHONG, Xiaotian , KRIZ, Ronald, William
CPC分类号： C07K1/13 , C12N9/12 , C12P21/02
摘要： The present invention is based on observations that transiently transfecting large- scale eukaryotic cell cultures with a polynucleotide encoding a protein of interest can be used to rapidly produce the large quantities of labeled proteins required for various biochemical techniques such as spectroscopy, microscopy, and crystallography, and applications including protein structure determination, protein tracing and/or localization, diagnostic and therapeutic applications, and affinity experiments. Thus, the present invention provides methods for rapidly producing large quantities of labeled proteins by using transient transfection of large-scale eukaryotic cell cultures, which are then grown in a chemically defined labeling medium that includes labeled amino acids. The present invention is also directed to methods of using the labeled proteins produced by the novel labeling methods for use in various techniques.
摘要翻译：本发明基于以下观察结果，即用编码感兴趣蛋白质的多核苷酸瞬时转染大规模真核细胞培养物可用于快速产生各种生物化学技术所需的大量标记蛋白质如光谱学，显微镜和晶体学，以及包括蛋白质结构测定，蛋白质追踪和/或定位，诊断和治疗应用以及亲和力实验在内的应用。因此，本发明提供了通过使用大规模真核细胞培养物的瞬时转染来快速产生大量标记蛋白质的方法，所述大规模真核细胞培养物然后在包含标记的氨基酸的化学确定的标记介质中生长。本发明还涉及使用由新颖标记方法产生的标记蛋白质用于各种技术的方法。

5. 发明申请

WO2006028889A2 SYSTEMS AND METHODS FOR PROTEIN PRODUCTION 审中-公开
标题翻译：蛋白质生产的系统和方法
公开(公告)号：WO2006028889A2
公开(公告)日：2006-03-16
申请号：PCT/US2005/031081
申请日：2005-09-01
申请人： WYETH , GAO, Yijie , PICHE, Nicole, M. , GENG, Mei , HERRMANN, Stephen, H. , ZHONG, Xiaotian , KRIZ, Ronald , LU, Zhijian
发明人： GAO, Yijie , PICHE, Nicole, M. , GENG, Mei , HERRMANN, Stephen, H. , ZHONG, Xiaotian , KRIZ, Ronald , LU, Zhijian
CPC分类号： C07K14/4705 , A01K2217/075 , C07K14/51 , C12P21/02
摘要： The invention relates to systems and methods for producing proteins of interest. The invention employs genetically-engineered animal or plant cells that have modified protein folding or processing capacities. In one aspect, the invention features genetically-engineered cells comprising one or more recombinant expression cassettes which encode (1) a protein of interest and (2) a polypeptide that is functional in the unfolded protein response (UPR) pathway of the cells. Co-expression of the polypeptide significantly increases the yield of the protein of interest in the genetically-engineered cells. In one example, the genetically-engineered cells aare animal cells, and the co-expressed polypeptide is a component or modulator of an XBP1- or ATF6- mediated UPR pathway.
摘要翻译：本发明涉及生产目标蛋白质的系统和方法。本发明使用具有改变的蛋白质折叠或加工能力的遗传工程化的动物或植物细胞。一方面，本发明的特征在于遗传工程改造的细胞，其包含编码（1）目的蛋白质的一种或多种重组表达盒，和（2）在细胞的未折叠蛋白质应答（UPR）途径中有功能的多肽。多肽的共表达显着提高了遗传工程细胞中目标蛋白的产量。在一个实例中，遗传工程改造的细胞是动物细胞，共表达的多肽是XBP1或ATF6介导的UPR途径的组分或调节剂。

6. 发明授权

EP1784490B1 SYSTEMS AND METHODS FOR PROTEIN PRODUCTION 有权转让
标题翻译：用于蛋白质生产的系统和方法
公开(公告)号：EP1784490B1
公开(公告)日：2017-11-01
申请号：EP05807248.9
申请日：2005-09-01
申请人： Wyeth LLC
发明人： GAO, Yijie , PICHE, Nicole, M. , GENG, Mei , HERRMANN, Stephen, H. , ZHONG, Xiaotian , KRIZ, Ronald , LU, Zhijian
IPC分类号： C12N15/82 , C12N15/63 , C12N15/85
CPC分类号： C07K14/4705 , A01K2217/075 , C07K14/51 , C12P21/02

7. 发明公开

EP1979376A2 STRUCTURE LINGO-1 审中-公开
标题翻译： STRUKTUR-LINGO-1
公开(公告)号：EP1979376A2
公开(公告)日：2008-10-15
申请号：EP07717194.0
申请日：2007-02-01
申请人： Wyeth
发明人： MOSYAK, Lidia , DWYER, Brian , JOHNSON, Mark , ZHONG, Xiaotian , PRESMAN, Eleonora , WILHELM, James M. , STAHL, Mark , SOMERS, William Stuart
IPC分类号： C07K14/43
CPC分类号： C07K14/4703 , C07K14/705 , C07K2299/00
摘要： This disclosure relates to LINGO-1 polypeptides, LINGO-1 polypeptide/ligand complexes, crystals of LINGO-1 polypeptides, crystals of LINGO-1 polypeptide/ligand complexes, and related methods and software systems.

8. 发明公开

EP1917354A2 AGGRECANASE STRUCTURE 审中-公开
标题翻译：聚集蛋白聚糖酶STRUKTUR
公开(公告)号：EP1917354A2
公开(公告)日：2008-05-07
申请号：EP06824853.3
申请日：2006-08-24
申请人： Wyeth
发明人： MOSYAK, Lidia , RUSH III, Thomas Saltmarsh , ZHONG, Xiaotian , MCDONAGH, Thomas E. , GEORGIADIS, Katy E. , SUM, Phaik-Eng , LAVALLIE, Edward R. , COLLINS-RACIE, Lisa A. , CORCORAN, Christopher John , KUMAR, Ravindra , HEBERT, Tracy , OLLAND, Stephane Hubert , MACKIE, Stewart Andrews
IPC分类号： C12N9/64 , G01N23/20 , G06F19/00 , C30B7/00
CPC分类号： C12N9/6421 , C07K2299/00 , C30B7/00
摘要： This invention relates to aggrecanase polypeptides and aggrecanase polypeptide/ligand complexes, crystals of aggrecanase and aggrecanase polypeptide/ligand complexes, and related methods and software systems.

9. 发明公开

EP1784490A2 SYSTEMS AND METHODS FOR PROTEIN PRODUCTION 有权转让
标题翻译： SYSTEME UND VERFAHREN ZUR PROTEINPRODUKTION
公开(公告)号：EP1784490A2
公开(公告)日：2007-05-16
申请号：EP05807248.9
申请日：2005-09-01
申请人： Wyeth
发明人： GAO, Yijie , PICHE, Nicole, M. , GENG, Mei , HERRMANN, Stephen, H. , ZHONG, Xiaotian , KRIZ, Ronald , LU, Zhijian
IPC分类号： C12N15/82 , C12N15/63 , C12N15/85
CPC分类号： C07K14/4705 , A01K2217/075 , C07K14/51 , C12P21/02
摘要： The invention relates to systems and methods for producing proteins of interest. The invention employs genetically-engineered animal or plant cells that have modified protein folding or processing capacities. In one aspect, the invention features genetically-engineered cells comprising one or more recombinant expression cassettes which encode (1) a protein of interest and (2) a polypeptide that is functional in the unfolded protein response (UPR) pathway of the cells. Co-expression of the polypeptide significantly increases the yield of the protein of interest in the genetically-engineered cells. In one example, the genetically-engineered cells aare animal cells, and the co-expressed polypeptide is a component or modulator of an XBP1- or ATF6- mediated UPR pathway.
摘要翻译：本发明涉及生产目的蛋白质的系统和方法。本发明使用具有改变的蛋白质折叠或加工能力的遗传工程化的动物或植物细胞。在一个方面，本发明的特征在于遗传工程改造的细胞，其包含一个或多个重组表达盒，所述重组表达盒编码（1）目的蛋白质和（2）在细胞的未折叠蛋白质应答（UPR）途径中有功能的多肽。多肽的共表达显着提高了遗传工程细胞中目标蛋白的产量。在一个实例中，遗传工程改造的细胞是动物细胞，共同表达的多肽是XBP1或ATF6介导的UPR途径的组分或调节剂。

10. 发明公开

EP1979372A2 METHODS OF LABELING TRANSIENTLY EXPRESSED PROTEINS IN LARGE-SCALE EUKARYOTIC CELL CULTURES 审中-公开
标题翻译：标记方法临时表达在真核细胞的大量培养物蛋白质
公开(公告)号：EP1979372A2
公开(公告)日：2008-10-15
申请号：EP07866980.1
申请日：2007-01-09
申请人： Wyeth
发明人： ZHONG, Xiaotian , KRIZ, Ronald William
IPC分类号： C07K1/13 , C12N9/12 , C12P21/02
CPC分类号： C07K1/13 , C12N9/12 , C12P21/02
摘要： The present invention is based on observations that transiently transfecting large- scale eukaryotic cell cultures with a polynucleotide encoding a protein of interest can be used to rapidly produce the large quantities of labeled proteins required for various biochemical techniques such as spectroscopy, microscopy, and crystallography, and applications including protein structure determination, protein tracing and/or localization, diagnostic and therapeutic applications, and affinity experiments. Thus, the present invention provides methods for rapidly producing large quantities of labeled proteins by using transient transfection of large-scale eukaryotic cell cultures, which are then grown in a chemically defined labeling medium that includes labeled amino acids. The present invention is also directed to methods of using the labeled proteins produced by the novel labeling methods for use in various techniques.

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