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1. 发明授权

US09784747B2 Method and kit for analyzing protein-protein interaction using nanocluster formation 有权
公开(公告)号：US09784747B2
公开(公告)日：2017-10-10
申请号：US14234243
申请日：2012-07-23
申请人： Won Do Heo , Sun Chang Kim , Sang Kyu Lee
发明人： Won Do Heo , Sun Chang Kim , Sang Kyu Lee
IPC分类号： G01N33/68 , C12N15/10 , C12N15/79 , G01N33/58
CPC分类号： G01N33/6845 , C07K2319/00 , C07K2319/60 , C07K2319/735 , C12N15/1055 , C12N15/79 , G01N33/582 , G01N2333/91205
摘要： For efficient analysis of a protein-protein interaction, the present disclosure provides a kit for analyzing a protein-protein interaction, the kit including: a 1st expression vector including a 1st polynucleotide and a multi-cloning site, wherein, the 1st polynucleotide is operably linked to a promoter and encodes a 1st fusion protein having a 1st fluorescence protein and a 1st self-assembly protein, and the multi-cloning site is a site where a polynucleotide encoding a bait protein may be operably linked to the polynucleotide encoding the 1st fusion protein; and a 2nd expression vector including a 2nd polynucleotide and a multi-cloning site, wherein, the 2nd polynucleotide is operably linked to a promoter and encodes a 2nd fusion protein having a 2nd fluorescence protein and a 2nd self-assembly protein, and the multi-cloning site is a site where a polynucleotide encoding a prey protein may be operably linked to the polynucleotide encoding the 2nd fusion protein.

2. 发明申请

US20150044712A1 METHOD AND KIT FOR ANALYZING PROTEIN-PROTEIN INTERACTION USING NANOCLUSTER FORMATION 有权
标题翻译：使用纳米聚糖形成分析蛋白质 - 蛋白质相互作用的方法和工具包
公开(公告)号：US20150044712A1
公开(公告)日：2015-02-12
申请号：US14234243
申请日：2012-07-23
申请人： Won Do Heo , Sun Chang Kim , Sang Kyu Lee
发明人： Won Do Heo , Sun Chang Kim , Sang Kyu Lee
IPC分类号： G01N33/68 , G01N33/58
CPC分类号： G01N33/6845 , C07K2319/00 , C07K2319/60 , C07K2319/735 , C12N15/1055 , C12N15/79 , G01N33/582 , G01N2333/91205
摘要： For efficient analysis of a protein-protein interaction, the present disclosure provides a kit for analyzing a protein-protein interaction, the kit including: a 1st expression vector including a 1st polynucleotide and a multi-cloning site, wherein, the 1st polynucleotide is operably linked to a promoter and encodes a 1st fusion protein having a 1st fluorescence protein and a 1st self-assembly protein, and the multi-cloning site is a site where a polynucleotide encoding a bait protein may be operably linked to the polynucleotide encoding the 1st fusion protein; and a 2nd expression vector including a 2nd polynucleotide and a multi-cloning site, wherein, the 2nd polynucleotide is operably linked to a promoter and encodes a 2nd fusion protein having a 2nd fluorescence protein and a 2nd self-assembly protein, and the multi-cloning site is a site where a polynucleotide encoding a prey protein may be operably linked to the polynucleotide encoding the 2nd fusion protein.
摘要翻译：为了有效分析蛋白质 - 蛋白质的相互作用，本公开提供了一种用于分析蛋白质 - 蛋白质相互作用的试剂盒，所述试剂盒包括：包含第一多核苷酸和多克隆位点的第一表达载体，其中第一多核苷酸可操作地连接到启动子并编码具有第一荧光蛋白和第一自组装蛋白的第一融合蛋白，并且多克隆位点是其中编码诱饵蛋白的多核苷酸可操作地连接到编码第一融合物的多核苷酸的位点蛋白; 和包含第二多核苷酸和多克隆位点的第二表达载体，其中第二多核苷酸可操作地连接于启动子并编码具有第二荧光蛋白和第二自组装蛋白的第二融合蛋白，克隆位点是编码猎物蛋白的多核苷酸可以可操作地连接到编码第二融合蛋白的多核苷酸的位点。

3. 发明申请

US20100150926A1 FUSION PROTEIN CAPABLE OF BINDING VEGF-A AND TNF-ALPHA 有权
标题翻译：融合蛋白能够结合VEGF-A和TNF-α
公开(公告)号：US20100150926A1
公开(公告)日：2010-06-17
申请号：US12636013
申请日：2009-12-11
申请人： Keehoon JUNG , Young Jun Koh , Gyun Min Lee , Sun Chang Kim , Gou Young Koh
发明人： Keehoon JUNG , Young Jun Koh , Gyun Min Lee , Sun Chang Kim , Gou Young Koh
IPC分类号： A61K39/395 , C07K16/00 , C07H21/04 , C12N15/63 , C12N5/10 , C12P21/06 , A61P19/02
CPC分类号： C07K16/241 , C07K14/71 , C07K14/715 , C07K16/22 , C07K2317/31 , C07K2319/70
摘要： The present application describes an isolated nucleic acid molecule encoding a polypeptide capable of synchronously binding VEGF polypeptide and TNF polypeptide comprising: (a) a nucleotide sequence encoding a TNFR2 component and VEGFR1 component operatively linked to (b) a nucleotide sequence encoding a multimerizing component, wherein the TNFR2 component consists essentially of a nucleotide sequence encoding the amino acid sequences of cystein rich domain 1, cystein rich domain 2, cystein rich domain 3, and cystein rich domain 4 of the extracellular domain of TNFR2, and wherein the VEGFR1 component consists essentially of a nucleotide sequence encoding the amino acid sequences of Ig-like domain 2 of the extracellular domain of VEGFR1.
摘要翻译：本申请描述了编码能够同时结合VEGF多肽和TNF多肽的多肽的分离的核酸分子，其包含：（a）编码TNFR2组分的核苷酸序列和可操作地连接到（b）编码多聚化成分的核苷酸序列的VEGFR1组分，其中TNFR2组分基本上由编码TNFR2细胞外结构域的富含半胱氨酸域1，半胱氨酸富含结构域2，半胱氨酸富含结构域3和半胱氨酸富含结构域4的氨基酸序列的核苷酸序列组成，其中VEGFR1组分基本上组成的编码VEGFR1细胞外结构域的Ig样结构域2的氨基酸序列的核苷酸序列。

4. 发明申请

US20100330623A1 COMPOSITIONS AND METHODS FOR AMINO ACID BIOSYNTHESIS 有权
标题翻译：氨基酸生物合成的组合物和方法
公开(公告)号：US20100330623A1
公开(公告)日：2010-12-30
申请号：US12863958
申请日：2009-02-19
申请人： Frederick R. Blattner , Sun Chang Kim , Jun Hyoung Lee
发明人： Frederick R. Blattner , Sun Chang Kim , Jun Hyoung Lee
IPC分类号： C12P13/24 , C12P13/04 , C12P13/22 , C12P13/20 , C12P13/14 , C12P13/12 , C12P13/10 , C12P13/08 , C12P13/06 , C12N1/21 , C12N15/63 , C07H21/04 , C07H21/00
CPC分类号： C12N9/93 , C12P13/04
摘要： The present invention relates generally to compositions and methods useful for, inter alia, production of commercial biologic products such as amino acids. More specifically, the present invention relates to genetically modified strains of microorganisms and the use thereof for the production of commercial products. The present invention also provides, inter alia, novel isolated DNA, nucleic acid, vectors and reduced genome bacteria.
摘要翻译：本发明一般涉及用于生产商业生物制品例如氨基酸的组合物和方法。更具体地，本发明涉及转基因微生物菌株及其用于生产商业产品的用途。本发明尤其提供了新的分离的DNA，核酸，载体和还原的基因组细菌。

5. 发明授权

US07790241B2 Steel tube having improved corrosion-resistance and method for manufacturing the same 有权
标题翻译：具有改进的耐腐蚀性的钢管及其制造方法
公开(公告)号：US07790241B2
公开(公告)日：2010-09-07
申请号：US11428408
申请日：2006-07-03
申请人： Gi Hyeoug Kim , Sun Chang Kim , Jae Pyeong Sim
发明人： Gi Hyeoug Kim , Sun Chang Kim , Jae Pyeong Sim
IPC分类号： B05D1/18
CPC分类号： C23C2/006 , C23C2/00 , C23C2/02 , C23C2/12 , Y10T29/4998 , Y10T29/5185
摘要： Disclosed is a steel tube with superior corrosion-resistance treated with plating and a manufacturing method of the same. The manufacturing method of the steel tube includes the steps of: preheating a steel tube formed through a milling process; maintaining the temperature of the preheated steel tube above a predetermined temperature, and creating a reduction atmosphere; melting an Al—Zn alloy containing 55 wt % of aluminum and 43.4-44.9 wt. % of zinc, and plating the molten alloy over the surface of the steel tube; cooling the steel tube; and coating the surface of the steel tube with a resin.
摘要翻译：公开了一种用电镀处理的具有优异耐腐蚀性的钢管及其制造方法。钢管的制造方法包括以下步骤：预热通过铣削加工形成的钢管; 将预热钢管的温度保持在预定温度以上，形成还原气氛; 熔化含有55重量％的铝和43.4-44.9重量％的Al-Zn合金。％的锌，并将熔融合金镀在钢管的表面上; 冷却钢管; 并用树脂涂覆钢管表面。

6. 发明授权

US07739980B2 Apparatus for manufacturing steel tube and method for manufacturing the same 失效
标题翻译：钢管制造装置及其制造方法
公开(公告)号：US07739980B2
公开(公告)日：2010-06-22
申请号：US11465813
申请日：2006-08-21
申请人： Gi Hyeoug Kim , Kyung Yun Kim , Yong Kil Kim , Sun Chang Kim , Jae Pyeong Sim , Jong Seo Kim
发明人： Gi Hyeoug Kim , Kyung Yun Kim , Yong Kil Kim , Sun Chang Kim , Jae Pyeong Sim , Jong Seo Kim
IPC分类号： B05C3/12
CPC分类号： C23C2/006 , C23C2/00 , C23C2/02 , C23C2/12 , Y10T29/4998 , Y10T29/5185
摘要： An apparatus and method for manufacturing a steel tube are provided. The apparatus includes: a tube-forming device for forming a steel plate into a steel tube; a heat treatment device connected in-line to the tube-forming device to heat the steel tube to a high temperature; a pre-treatment device for annealing the steel tube and providing a reduction atmosphere; and a plating device including a pot for storing a SeAHLume alloy composed of aluminum and zinc in a molten state, a level block selectively inserted into the molten alloy to adjust a level of the molten alloy, and a plating part into which the molten alloy flows in response to insertion of the level block and through which the steel tube passes substantially vertically. Therefore, it is possible to manufacture a steel tube having a plated surface to improve its corrosion-resistance.
摘要翻译：提供一种用于制造钢管的设备和方法。该装置包括：用于将钢板形成钢管的管形成装置; 与管形成装置成直线连接的热处理装置，将钢管加热到高温; 用于退火钢管并提供还原气氛的预处理装置; 以及电镀装置，其包括用于储存熔融状态的由铝和锌组成的SeAHLume合金的罐，选择性地插入熔融合金中的级块，以调节熔融合金的水平，以及熔融合金流入的镀层响应于水平块的插入，并且钢管通过其基本垂直地穿过。因此，可以制造具有电镀表面的钢管，以提高其耐腐蚀性。

7. 发明授权

US09067970B2 Anticancer agents comprising peptides with cancer-specific toxicity 有权
标题翻译：包含具有癌症特异性毒性的肽的抗癌剂
公开(公告)号：US09067970B2
公开(公告)日：2015-06-30
申请号：US13700722
申请日：2010-12-24
申请人： Sun Chang Kim , Su A Jang , Da Jung Kim , Bong Hyun Sung , Ki Jeong Lim , Ju Ri Shin , Young Woong Lee
发明人： Sun Chang Kim , Su A Jang , Da Jung Kim , Bong Hyun Sung , Ki Jeong Lim , Ju Ri Shin , Young Woong Lee
IPC分类号： G01N33/574 , A61K38/10 , C07K7/08 , G01N33/53
CPC分类号： C07K7/08 , A61K38/10 , G01N33/53 , G01N33/574
摘要： The present invention relates a prophylactic or therapeutic composition for cancer, and more particularly, to a prophylactic or therapeutic composition for cancer comprising a peptide which is represented by an amino acid sequence of the following Formula (I), a method for preventing or treating cancer comprising the step of administering the peptide to a subject, and use of the peptide in the preparation of the prophylactic or therapeutic composition for cancer. (I) APKAMX1LLX2X3L-LX4LQKKGI wherein X1, X2, X3 and X4 are each independently R or K.
摘要翻译：本发明涉及用于癌症的预防或治疗组合物，更具体地，涉及用于癌症的预防或治疗组合物，其包含由下式（I）的氨基酸序列表示的肽，预防或治疗癌症的方法包括向受试者施用该肽的步骤，以及该肽在制备用于癌症的预防或治疗组合物中的用途。（I）APKAMX1LLX2X3L-LX4LQKKGI其中X1，X2，X3和X4各自独立地为R或K.

8. 发明授权

US08927232B2 Fusion protein capable of binding VEGF-A and TNF-alpha 有权
标题翻译：能够结合VEGF-A和TNF-α的融合蛋白
公开(公告)号：US08927232B2
公开(公告)日：2015-01-06
申请号：US12636013
申请日：2009-12-11
申请人： Keehoon Jung , Young Jun Koh , Gyun Min Lee , Sun Chang Kim , Gou Young Koh
发明人： Keehoon Jung , Young Jun Koh , Gyun Min Lee , Sun Chang Kim , Gou Young Koh
IPC分类号： A61K38/17 , C07K14/71 , C07K14/715 , C12N5/10 , C12N15/12
CPC分类号： C07K16/241 , C07K14/71 , C07K14/715 , C07K16/22 , C07K2317/31 , C07K2319/70
摘要： The present application describes an isolated nucleic acid molecule encoding a polypeptide capable of synchronously binding VEGF polypeptide and TNF polypeptide comprising: (a) a nucleotide sequence encoding a TNFR2 component and VEGFR1 component operatively linked to (b) a nucleotide sequence encoding a multimerizing component, wherein the TNFR2 component consists essentially of a nucleotide sequence encoding the amino acid sequences of cystein rich domain 1, cystein rich domain 2, cystein rich domain 3, and cystein rich domain 4 of the extracellular domain of TNFR2, and wherein the VEGFR1 component consists essentially of a nucleotide sequence encoding the amino acid sequences of Ig-like domain 2 of the extracellular domain of VEGFR1.
摘要翻译：本申请描述了编码能够同时结合VEGF多肽和TNF多肽的多肽的分离的核酸分子，其包含：（a）编码TNFR2组分的核苷酸序列和可操作地连接到（b）编码多聚化成分的核苷酸序列的VEGFR1组分，其中TNFR2组分基本上由编码TNFR2细胞外结构域的富含半胱氨酸域1，半胱氨酸富含结构域2，半胱氨酸富含结构域3和半胱氨酸富含结构域4的氨基酸序列的核苷酸序列组成，其中VEGFR1组分基本上组成的编码VEGFR1细胞外结构域的Ig样结构域2的氨基酸序列的核苷酸序列。

9. 发明授权

US08735129B2 Ginsenoside glycosidase derived from the genus Terrabacter, and use thereof 有权
标题翻译：衍生自Terrabacter属的人参皂苷糖苷酶及其用途
公开(公告)号：US08735129B2
公开(公告)日：2014-05-27
申请号：US13453893
申请日：2012-04-23
申请人： Dong Shan An , Song Gun Kim , Sung Taik Lee , Wan Taek Im , Hyung Gwan Lee , Sun Chang Kim
发明人： Dong Shan An , Song Gun Kim , Sung Taik Lee , Wan Taek Im , Hyung Gwan Lee , Sun Chang Kim
IPC分类号： C12N9/24 , C12P33/20 , C12N1/20 , C12N15/00
CPC分类号： C12P33/00 , C12N9/2445 , C12Y302/01021
摘要： The present invention relates to a novel ginsenoside glycosidase protein derived from the genus Terrabacter, the protein having an activity of converting protopanaxadiol (PPD)-type saponins into highly active substances, which can be absorbed inside the body, by selective hydrolysis of a particular bond of ginsenoside. More specifically, the present invention relates to an amino acid sequence of the protein, a nucleic acid sequence encoding the protein, a recombinant vector comprising the nucleic acid sequence, and a transformant transformed with the vector, and a method for producing ginsenoside glycosidase derived from the genus Terrabacter by culturing the transformant, a method for converting PPD-type major saponins into the minor saponin forms using the protein, and a composition for converting PPD-type saponins into soluble saponins, comprising the protein as an active component.
摘要翻译：本发明涉及一种衍生自Terrabacter属的新型人参皂苷糖苷酶蛋白质，该蛋白质具有将原人参二醇（PPD）型皂甙转化为高活性物质的活性，其可通过选择性水解特定的键的人参皂苷。更具体地说，本发明涉及蛋白质的氨基酸序列，编码该蛋白质的核酸序列，包含该核酸序列的重组载体和用该载体转化的转化体，以及一种用于生产人参皂苷糖苷酶的方法，其衍生自通过培养转化体的Terrabacter属，使用该蛋白质将PPD型主皂苷转化成次皂苷形式的方法，以及将PPD型皂苷转化为可溶性皂苷的组合物，其包含该蛋白质作为活性成分。

10. 发明授权

US08003348B2 Method for the mass expression of an antimicrobial peptide by co-expression of a basic antimicrobial peptide and an acidic peptide using a translational coupling system 有权
标题翻译：通过使用平移耦合系统共同表达碱性抗微生物肽和酸性肽来大量表达抗微生物肽的方法
公开(公告)号：US08003348B2
公开(公告)日：2011-08-23
申请号：US12313513
申请日：2008-11-19
申请人： Sun Chang Kim , Su A Jang , Bong Hyun Sung , Jung Min Kim , Ki Jung Lim , Ju Ri Shin , Ju Young Lee
发明人： Sun Chang Kim , Su A Jang , Bong Hyun Sung , Jung Min Kim , Ki Jung Lim , Ju Ri Shin , Ju Young Lee
IPC分类号： C12P21/06 , C12P21/04
CPC分类号： C12N15/67 , C07K14/4702
摘要： The present invention relates to a gene construct which is capable of achieving efficient production of an antimicrobial peptide in a microorganism, and a method for efficient mass production and separation of an antimicrobial peptide using the same. The gene construct of the present invention has a translationally coupled configuration of two independent and separate cistrons which encode an acidic peptide and a basic antimicrobial peptide, each having an opposite charge, under the control of a single promoter. The translationally coupled acidic peptide and basic antimicrobial peptide undergo charge-charge interaction simultaneously with expression thereof to neutralize the potential cytotoxicity of the antimicrobial peptide, resulting in prevention of antimicrobial peptide-mediated killing of host microorganisms. In addition, a conjugate of the acidic peptide and the antimicrobial peptide can be separated without chemical or enzymatic treatment. Therefore, it is possible to achieve easy mass production of antimicrobial peptides from recombinant microorganisms.
摘要翻译：本发明涉及能够实现微生物中抗微生物肽的高效生产的基因构建体，以及使用该抗体肽高效批量生产和分离的方法。本发明的基因构建体具有两个独立和分离的顺反子的翻译偶联构型，其在单个启动子的控制下编码各自具有相反电荷的酸性肽和碱性抗微生物肽。翻译偶联的酸性肽和碱性抗微生物肽与其表达同时进行电荷 - 电荷相互作用以中和抗微生物肽的潜在细胞毒性，从而防止抗微生物肽介导的宿主微生物的杀伤。此外，酸性肽和抗微生物肽的缀合物可以在没有化学或酶处理的情况下分离。因此，可以从重组微生物容易地大量生产抗微生物肽。

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