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1. 发明申请

US20130203969A1 USE OF SMALL MOLECULES IN METHODS FOR PURIFICATION OF BIOMOLECULES 审中-公开
标题翻译：在生物分子纯化方法中使用小分子
公开(公告)号：US20130203969A1
公开(公告)日：2013-08-08
申请号：US13566320
申请日：2012-08-03
申请人： Jad Jaber , Wilson Moya , Ajish Potty , Alison Dupont , Matthew T. Stone , Mikhail Kozlov
发明人： Jad Jaber , Wilson Moya , Ajish Potty , Alison Dupont , Matthew T. Stone , Mikhail Kozlov
IPC分类号： C07K16/00
CPC分类号： C07K16/00 , B01D15/327 , B01D15/361 , B01D15/3804 , B01D15/3847 , C07K1/30
摘要： The present invention relates to novel and improved methods for the purification of biomolecules. In particular, the present invention relates to methods of protein purification which employ small molecules, which include at least one non-polar group and at least one cationic group or which include at least one non-polar group and at least one anionic group.
摘要翻译：本发明涉及用于纯化生物分子的新颖和改进的方法。特别地，本发明涉及使用包括至少一个非极性基团和至少一个阳离子基团或包括至少一个非极性基团和至少一个阴离子基团的小分子的蛋白质纯化方法。

2. 发明申请

US20110020327A1 Purification of proteins 审中-公开
标题翻译：蛋白质的纯化
公开(公告)号：US20110020327A1
公开(公告)日：2011-01-27
申请号：US12592744
申请日：2009-12-02
申请人： Wilson Moya , Jad Jaber
发明人： Wilson Moya , Jad Jaber
IPC分类号： A61K39/395 , C07K1/14 , C07K16/18 , C07K16/30 , C07K16/22 , C07K14/62 , C07K14/61 , C12N9/96 , C07K14/505 , A61K38/43 , A61K38/00
CPC分类号： C07K1/32
摘要： The present invention relates to a bimodal polymer such as a soluble polymer capable of irreversibly binding to insoluble particulates and a subset of soluble impurities and also capable of reversibly binding to one or more desired biomolecules in an unclarified biological material containing stream and the methods of using such a material to purify one or more desired biomolecules from such a stream without the need for prior clarification. Such a polymer comprises domains of charged pendant groups such as primary, secondary, tertiary or quaternary amines, (first mode) and is rendered insoluble and precipitates out of solution simply upon complexing with oppositely charged solid particulates and a fraction of the soluble impurities in an amount sufficient to form an aggregate that can no longer be held in solution. The polymer further comprises other domains of pendant groups that are charged or uncharged, hydrophilic or hydrophobic or have a ligand that is selective for the biomolecule of interest depending on the process conditions such as pH, ionic strength, salts, and the like (second mode). When present in one mode, such as the uncharged form, said pendant groups are capable of binding to one or more desired biomolecules within the stream (protein, polypeptide, etc) in an unclarified cell broth. The precipitate can then be removed from the stream, such as by being filtered out from the remainder of the stream and the desired biomolecule is recovered such as by selective elution.
摘要翻译：本发明涉及双峰聚合物，例如能够不可逆地结合不溶性微粒的可溶性聚合物和可溶性杂质的子集，并且还能够在含有未澄清的含生物材料的物流中可逆地结合一种或多种所需的生物分子，以及使用这种材料从这种流中纯化一种或多种所需的生物分子，而不需要事先澄清。这种聚合物包括带电侧基的结构域，例如伯，仲，叔或季胺，（第一模式），并且在与相反电荷的固体颗粒和一部分可溶性杂质在足以形成不能再溶解的聚集体。聚合物还包括根据诸如pH，离子强度，盐等的工艺条件，带电荷或不带电荷，亲水或疏水的或具有对感兴趣的生物分子有选择性的配体的侧基的其它结构域（第二模式）。当以一种模式存在时，例如不带电荷的形式，所述侧基能够与未澄清的细胞液中的流（蛋白质，多肽等）内的一种或多种所需的生物分子结合。然后可以从流中除去沉淀物，例如通过从其余流中过滤出来，并且通过选择性洗脱来回收所需的生物分子。

3. 发明申请

US20080255027A1 Purification of proteins 有权
标题翻译：蛋白质的纯化
公开(公告)号：US20080255027A1
公开(公告)日：2008-10-16
申请号：US12004319
申请日：2007-12-20
申请人： Wilson Moya , Jad Jaber
发明人： Wilson Moya , Jad Jaber
IPC分类号： A61K38/02 , B01D15/08 , C07K1/32
CPC分类号： C07K1/32 , C07K1/30
摘要： The present invention relates to a selectively soluble polymer capable of binding to a desired biomolecules in a mixture containing various biological materials and the methods of using such a polymer to purify a biomolecule from such a mixture. The polymer is soluble in the mixture under a certain set of process conditions such as pH or temperature and/or salt concentration and is rendered insoluble and precipitates out of solution upon a change in the process conditions. The polymer is capable of binding to the desired biomolecule (protein, polypeptide, etc) and remains capable of binding to that biomolecule even after the polymer is precipitated out of solution. The precipitate can then be filtered out from the remainder of the stream and the desired biomolecule is recovered such as by elution and further processed.
摘要翻译：本发明涉及能够在含有各种生物材料的混合物中结合所需生物分子的选择性可溶性聚合物以及使用这种聚合物从这种混合物中纯化生物分子的方法。聚合物在一定的工艺条件例如pH或温度和/或盐浓度下可溶于混合物中，并且在工艺条件改变时变得不溶解并沉淀出溶液。聚合物能够结合所需的生物分子（蛋白质，多肽等），并且即使在聚合物从溶液中沉淀出来之后仍然能够结合该生物分子。然后可以从流的其余部分过滤出沉淀物，并回收所需的生物分子，例如通过洗脱和进一步处理。

4. 发明授权

US08691918B2 Stimulus responsive polymers for the purification of biomolecules 有权
标题翻译：用于生物分子纯化的刺激响应聚合物
公开(公告)号：US08691918B2
公开(公告)日：2014-04-08
申请号：US13108576
申请日：2011-05-16
申请人： Jad Jaber , Wilson Moya , James Hamzik , Arezki Boudif , Yu Zhang , Neil Soice , John Charkoudian , Nripen Singh
发明人： Jad Jaber , Wilson Moya , James Hamzik , Arezki Boudif , Yu Zhang , Neil Soice , John Charkoudian , Nripen Singh
IPC分类号： C08F16/00
CPC分类号： B01J41/14 , B01J41/13 , C07K1/30 , C07K1/32 , C07K16/00 , C07K2317/10 , C08F8/00 , C08F8/02 , C08F126/02 , C08F26/02
摘要： The present invention provides novel and improved stimulus responsive polymers and methods of using the same for the purification of biomolecules.
摘要翻译：本发明提供了新的和改进的刺激响应聚合物以及使用其进行生物分子纯化的方法。

5. 发明授权

US08362217B2 Purification of proteins 有权
标题翻译：蛋白质的纯化
公开(公告)号：US08362217B2
公开(公告)日：2013-01-29
申请号：US12004319
申请日：2007-12-20
申请人： Wilson Moya , Jad Jaber
发明人： Wilson Moya , Jad Jaber
IPC分类号： A61K39/395 , C07K1/32
CPC分类号： C07K1/32 , C07K1/30
摘要： The present invention relates to a selectively soluble polymer capable of binding to a desired biomolecules in a mixture containing various biological materials and the methods of using such a polymer to purify a biomolecule from such a mixture. The polymer is soluble in the mixture under a certain set of process conditions such as pH or temperature and/or salt concentration and is rendered insoluble and precipitates out of solution upon a change in the process conditions. The polymer is capable of binding to the desired biomolecule (protein, polypeptide, etc) and remains capable of binding to that biomolecule even after the polymer is precipitated out of solution. The precipitate can then be filtered out from the remainder of the stream and the desired biomolecule is recovered such as by elution and further processed.
摘要翻译：本发明涉及能够在含有各种生物材料的混合物中结合所需生物分子的选择性可溶性聚合物以及使用这种聚合物从这种混合物中纯化生物分子的方法。聚合物在一定的工艺条件例如pH或温度和/或盐浓度下可溶于混合物中，并且在工艺条件改变时变得不溶解并沉淀出溶液。聚合物能够结合所需的生物分子（蛋白质，多肽等），并且即使在聚合物从溶液中沉淀出来之后仍然能够结合该生物分子。然后可以从流的其余部分过滤出沉淀物，并回收所需的生物分子，例如通过洗脱和进一步处理。

6. 发明授权

US08569464B2 Purification of proteins 有权
公开(公告)号：US08569464B2
公开(公告)日：2013-10-29
申请号：US12316708
申请日：2008-12-16
申请人： Wilson Moya , Jad Jaber
发明人： Wilson Moya , Jad Jaber
IPC分类号： A23J1/00 , C07K16/00
CPC分类号： C07K1/32 , C07K1/30
摘要： The present invention relates to a selectively soluble polymer capable of binding to a desired molecules in an unclarified mixture containing various biological materials and the methods of using such a polymer to purify a molecule from such a mixture. The polymer is soluble in the mixture under a certain set of process conditions such as pH or temperature and/or salt concentration and is rendered insoluble and precipitates out of solution upon a change in the process conditions. The polymer is capable of binding to the desired molecule (protein, polypeptide, etc) and remains capable of binding to that molecule even after the polymer is precipitated out of solution. The precipitate can then be filtered out from the remainder of the stream and the desired biomolecule is recovered such as by elution and further processed.

7. 发明申请

US20110313066A1 STIMULUS RESPONSIVE POLYMERS FOR THE PURIFICATION OF BIOMOLECULES 有权
标题翻译：用于纯化生物分子的刺激性聚合物
公开(公告)号：US20110313066A1
公开(公告)日：2011-12-22
申请号：US13108576
申请日：2011-05-16
申请人： Jad Jaber , Wilson Moya , James Hamzik , Arezki Boudif , Yu Zhang , Neil Soice , John Charkoudian , Nripen Singh
发明人： Jad Jaber , Wilson Moya , James Hamzik , Arezki Boudif , Yu Zhang , Neil Soice , John Charkoudian , Nripen Singh
IPC分类号： B01J39/20 , C07K1/36 , C07K1/30
CPC分类号： B01J41/14 , B01J41/13 , C07K1/30 , C07K1/32 , C07K16/00 , C07K2317/10 , C08F8/00 , C08F8/02 , C08F126/02 , C08F26/02
摘要： The present invention provides novel and improved stimulus responsive polymers and methods of using the same for the purification of biomolecules.
摘要翻译：本发明提供了新的和改进的刺激响应聚合物以及使用其进行生物分子纯化的方法。

8. 发明申请

US20090232737A1 Purification of proteins 有权
标题翻译：蛋白质的纯化
公开(公告)号：US20090232737A1
公开(公告)日：2009-09-17
申请号：US12316708
申请日：2008-12-16
申请人： Wilson Moya , Jad Jaber
发明人： Wilson Moya , Jad Jaber
IPC分类号： A61K49/00 , C07K1/30 , A61K38/12 , A61P43/00 , A61K39/395
CPC分类号： C07K1/32 , C07K1/30
摘要： The present invention relates to a selectively soluble polymer capable of binding to a desired molecules in an unclarified mixture containing various biological materials and the methods of using such a polymer to purify a molecule from such a mixture. The polymer is soluble in the mixture under a certain set of process conditions such as pH or temperature and/or salt concentration and is rendered insoluble and precipitates out of solution upon a change in the process conditions. The polymer is capable of binding to the desired molecule (protein, polypeptide, etc) and remains capable of binding to that molecule even after the polymer is precipitated out of solution. The precipitate can then be filtered out from the remainder of the stream and the desired biomolecule is recovered such as by elution and further processed.
摘要翻译：本发明涉及能够在含有各种生物材料的未澄清混合物中结合所需分子的选择性可溶性聚合物以及使用这种聚合物从这种混合物中纯化分子的方法。聚合物在一定的工艺条件例如pH或温度和/或盐浓度下可溶于混合物中，并且在工艺条件改变时变得不溶解并沉淀出溶液。聚合物能够结合到所需的分子（蛋白质，多肽等），并且即使在聚合物从溶液中沉淀出来之后仍然能够结合该分子。然后可以从流的其余部分过滤出沉淀物，并回收所需的生物分子，例如通过洗脱和进一步处理。

9. 发明申请

US20120070836A1 METHODS OF DETECTING RESIDUAL AMOUNTS OF POLYMERS USED IN THE PURIFICATION OF BIOMOLECULES 审中-公开
标题翻译：检测生物分子纯化中使用聚合物残留量的方法
公开(公告)号：US20120070836A1
公开(公告)日：2012-03-22
申请号：US13155912
申请日：2011-06-08
申请人： Martin Zillmann , Jad Jaber , James Hamzik
发明人： Martin Zillmann , Jad Jaber , James Hamzik
IPC分类号： C12Q1/68 , G01N21/64 , G01N33/53 , C12Q1/02
CPC分类号： C12Q1/68 , Y10T436/143333 , C12Q2563/179
摘要： The present invention relates to methods of detecting residual amounts of polymers including stimulus responsive polymers, which are used in processes for purifying biomolecules such as, for example, proteins, polypeptides, antibodies, vaccines and the like.
摘要翻译：本发明涉及检测残余量的聚合物的方法，包括刺激反应性聚合物，其用于纯化生物分子例如蛋白质，多肽，抗体，疫苗等的方法。

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