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1. 发明授权

US08999295B2 Technique for drug and gene delivery to the cell cytosol 有权
标题翻译：药物和基因递送至细胞溶质的技术
公开(公告)号：US08999295B2
公开(公告)日：2015-04-07
申请号：US12940047
申请日：2010-11-05
申请人： William G. Pitt , Ghaleb Husseini
发明人： William G. Pitt , Ghaleb Husseini
IPC分类号： A61B8/00 , A61K9/127 , A61K47/06 , A61K9/107 , A61K31/7105 , A61K47/48
CPC分类号： A61K47/06 , A61K9/1075 , A61K9/1271 , A61K9/1277 , A61K31/7105 , A61K47/6911 , Y10S977/773 , Y10S977/907
摘要： Provided herein is a composition, a method, and a system for delivering a functional molecule to the cytosol of a cell, comprising a liposome configured to be taken into a cell, including by a process selected from the group consisting of endocytosis, pinocytosis or phagocytosis, the liposome comprising a phase transforming liquid with vapor pressure capable of forming a gas at low pressure, said liquid being associated with the liposome, and the liposome further comprising at least one functional molecule selected from the group consisting of a therapeutic molecule, a detectable label, and a targeting molecule.
摘要翻译：本文提供了一种用于将功能性分子递送至细胞的细胞溶质的组合物，方法和系统，所述组合物，方法和系统包括构建成被摄入细胞的脂质体，包括通过选自由内吞作用，胞饮作用或吞噬作用所述脂质体包含具有能够在低压下形成气体的蒸气压的相变液，所述液体与所述脂质体相关联，所述脂质体还包含至少一种选自以下的功能性分子：治疗分子，可检测的标签和靶向分子。

2. 发明授权

US06783525B2 Application and utilization of a water-soluble polymer on a surface 有权
标题翻译：表面上应用和利用水溶性聚合物
公开(公告)号：US06783525B2
公开(公告)日：2004-08-31
申请号：US10021532
申请日：2001-12-12
申请人： Darcy W. Greep , William G. Pitt
发明人： Darcy W. Greep , William G. Pitt
IPC分类号： A61B1818
CPC分类号： A61B18/14 , A61B2018/00083 , A61B2018/00142
摘要： Methods, systems, and devices for applying and utilizing a water-soluble polymer on a surface to provide desirable properties, attributes and/or characteristics to the surface. In a first embodiment, the water-soluble polymer at least partially fills one or more pores of a fluoropolymer or a porous metal. In a second implementation, the water-soluble polymer is mixed into a base material and the mixture is applied as a coating layer onto the surface. Where the surface is an electrosurgical electrode tip, the desirable properties, attributes and/or characteristics may include the ability to attract water that assists in cooling and/or lubricating the tip, to create a low shear, sacrificial layer that protects and enhances the tip, to supply a radical scavenger or inhibitor that reduces damage at the tip, and/or to deposit factors, such as healing factors, from the tip onto one or more contact surfaces of the patient's body.
摘要翻译：用于在表面上施用和利用水溶性聚合物以向表面提供所需性质，属性和/或特性的方法，系统和装置。在第一个实施方案中，水溶性聚合物至少部分地填充含氟聚合物或多孔金属的一个或多个孔。在第二个实施方案中，将水溶性聚合物混合到基材中，并将混合物作为涂层施加到表面上。当表面是电外科电极尖端时，期望的性质，属性和/或特征可以包括吸引有助于冷却和/或润滑尖端的水的能力，以产生保护和增强尖端的低剪切牺牲层，以提供一种自由基清除剂或抑制剂，其减少尖端处的损伤和/或将诸如愈合因子的因素从尖端沉积到患者身体的一个或多个接触表面上。

3. 发明授权

US06649702B1 Stabilization and acoustic activation of polymeric micelles for drug delivery 失效
标题翻译：用于药物递送的聚合胶束的稳定和声学活化
公开(公告)号：US06649702B1
公开(公告)日：2003-11-18
申请号：US09979481
申请日：2002-08-07
申请人： Natalya Rapoport , William G. Pitt
发明人： Natalya Rapoport , William G. Pitt
IPC分类号： C08L5300
CPC分类号： A61K9/5138 , A61K9/0009 , A61K9/1075 , A61K9/5146 , C08G65/32 , C08G2650/58 , C08L71/02 , C08L2666/04
摘要： Methods are disclosed in which a micelle is stabilized against degradation upon dilution. The micelle comprises molecules of a block polymer having a hydrophobic block and a hydrophilic block. The hydrophobic block forms a core of the micelle with corona from the hydrophilic block. The methods for stabilizing the core are (1) by chemically cross-linking, (2) incorporating a hydrophobic oil (vegetable oil) in the core to render it more hydrophobic and stable, and (3) incorporating a cross-linked interpenetrating network of a stimuli-responsive hydrogel into the core. The hydrogel is responsive to any stimuli, but preferably temperature or pH. A substance such as, drugs, can be incorporated into the dense inner core of the micelles. When subjected to ultrasound, the micelles release the substance, and then reversibly revert to a stable dense core and re-encapsulating the substance when the ultrasound is turned off. By pulsing the ultrasound, it is therefore, to controllably release the substance in a pulsed manner corresponding to the ultrasound signal.
摘要翻译：公开了在稀释时胶束稳定以免降解的方法。胶束包括具有疏水嵌段和亲水嵌段的嵌段聚合物的分子。疏水嵌段由亲水嵌段形成具有电晕的胶束核心。用于稳定核心的方法是（1）通过化学交联，（2）在核心中加入疏水性油（植物油）以使其更具疏水性和稳定性，和（3）结合交联的互穿网络刺激性反应性水凝胶进入核心。水凝胶对任何刺激都有响应，但优选温度或pH。药物等物质可以并入胶束的致密内核中。当进行超声波处理时，胶束释放物质，然后当超声波转动时，可逆地还原成稳定的致密核心并重新封装物质关闭通过脉冲超声波，因此可以以对应于超声信号的脉冲方式可控地释放物质。

4. 发明授权

US5108780A Enhanced thermoplastic adhesion to fibers by using plasma discharge 失效
标题翻译：通过使用等离子体放电增强纤维的热塑性附着力
公开(公告)号：US5108780A
公开(公告)日：1992-04-28
申请号：US647597
申请日：1991-01-28
申请人： William G. Pitt , John E. Lakenan
发明人： William G. Pitt , John E. Lakenan
IPC分类号： B29B15/08 , B29B15/12 , B29C59/14 , C08J5/06
CPC分类号： B29B15/08 , B29B15/125 , B29C59/14 , C08J5/06 , B29C2037/90
摘要： A device and method for enhancing adhesion between an embedded filler component and surrounding thermoplastic matrix which together form a composite material. The filler component is subjected to plasma discharge and subsequently maintained in an inert environment to protect the treated surface area against premature exposure to reactive substances. While maintained in this inert environment, the treated surface is coated with a thermoplastic polymer and solidified. This coated filler is then capable of processing into composite materials. The device comprises an enclosure which is adapted which includes a containment space and environment control for maintaining inert conditions during application of plasma discharge and coating with thermoplastic polymer.
摘要翻译：一种用于增强嵌入填料组分和周围热塑性基质之间的粘合力的装置和方法，其一起形成复合材料。对填料组分进行等离子体放电，随后保持在惰性环境中，以保护被处理的表面区域免于过早暴露于反应物质。在保持在该惰性环境中的同时，将处理过的表面涂覆有热塑性聚合物并固化。然后，该涂覆的填料能够加工成复合材料。该装置包括适于包括容纳空间和环境控制的外壳，用于在用热塑性聚合物施加等离子体放电和涂覆期间保持惰性条件。

5. 发明授权

US08413313B2 Divided-end artificial limb 有权
标题翻译：分割假肢
公开(公告)号：US08413313B2
公开(公告)日：2013-04-09
申请号：US12940909
申请日：2010-11-05
申请人： David Delon Williams , William G. Pitt , Peter Austin Jepsen , Douglas Turley Wright , Carl Jay Ellingson , Daniel August Susumu Marler
发明人： David Delon Williams , William G. Pitt , Peter Austin Jepsen , Douglas Turley Wright , Carl Jay Ellingson , Daniel August Susumu Marler
IPC分类号： A61F2/66 , A61F1/00
CPC分类号： B29C57/00 , A61F2/5044 , A61F2/66 , A61F2/80 , A61F2002/5003 , A61F2002/5056 , A61F2002/5072 , A61F2002/6621 , A61F2002/665 , A61F2002/6664 , B29C53/02 , B29C2793/0081 , B29L2031/7532 , Y10T29/49789 , Y10T29/49798 , Y10T29/49826 , Y10T29/49865 , Y10T29/49947 , Y10T29/49948 , Y10T29/4998
摘要： The present invention provides divided-end artificial limbs and methods for making divided-end artificial limbs that are inexpensive, simple, and customizable. A limb is formed by dividing an end of an elongated shaft member into extending prongs that are shaped into custom lengths and curvatures. Furthermore, the method of the present invention provides affixing a sole plate to the limb, reinforcement of the prongs by nesting additional prongs within the elongated shaft member, dividing the proximal end of the shaft member into circumferentially spaced-apart fingers, and dividing a midsection of the shaft member into circumferentially spaced-apart flexing vanes. The present invention also provides that the prongs may be further modified by division into toe and heel extensions or by wrapping a prong around another prong to form a sole. Methods of reducing stress concentration using bolts and stress relief holes in these limbs are also presented.
摘要翻译：本发明提供了分割端假肢和制造便宜，简单和可定制的分割假肢的方法。通过将细长轴构件的端部分成成为定制长度和曲率的延伸尖端而形成肢体。此外，本发明的方法提供了将底板固定在肢体上，通过在细长轴构件内嵌入额外的插脚来加强插脚，将轴构件的近端分成周向间隔开的手指，并将中间部分的轴构件形成周向间隔开的挠曲叶片。本发明还提供了，可以通过分割成脚趾和脚跟延伸部或通过围绕另一个插脚包裹叉子来进一步修改插脚以形成鞋底。还提出了使用这些肢体中的螺栓和应力释放孔来减少应力集中的方法。

6. 发明授权

US5739411A Accelerated impact testing apparatus 失效
标题翻译：加速冲击试验装置
公开(公告)号：US5739411A
公开(公告)日：1998-04-14
申请号：US768861
申请日：1996-12-17
申请人： Nathan J. Lee , William G. Pitt
发明人： Nathan J. Lee , William G. Pitt
IPC分类号： G01N3/48 , G01N3/52 , G01N33/00 , G01N3/00
CPC分类号： G01N3/48 , G01N3/52 , G01N2033/008
摘要： Accelerated impact testing apparatus which simulates the dynamic strain in golf clubs, shafts and other specimens by rapidly transferring kinetic energy to the specimen from an instrumented tup that is free to rebound. The specimen is held in place by two holding fixtures uniquely designed to provide the appropriate boundary and support conditions for the transient strain to propagate in a manner similar to that of the actual application e.g. a golf club striking a ball. Force applied to the tup is generated by compressing a spring to a predetermined length and releasing its energy through a rapidly actuated latch. Actual force applied as a function of time is measured by a load cell located in the tup and the tup velocity just prior to impact is computed from a velocity sensor signal. Acceleration of the impact event is also recorded as well as strain levels in the shaft.
摘要翻译：加速冲击试验装置，通过从能够自由反弹的仪表托盘快速将动能转移到试样上，模拟高尔夫球杆，轴和其他试样中的动态应变。样品通过两个固定装置固定在适当的位置，其独特地设计成为瞬时应变提供适当的边界和支撑条件，以与实际应用类似的方式传播。一个打高尔夫球的球。通过将弹簧压缩到预定长度并通过快速致动的闩锁释放其能量来产生施加到托盘上的力。作为时间的函数施加的实际力由位于tup中的测力传感器测量，并且刚好在冲击之前的速度传感器信号由速度传感器信号计算。还记录了冲击事件的加速度以及轴中的应变水平。

7. 发明申请

US20110107581A1 Divided-End Artificial Limb 有权
标题翻译：分割端人造肢体
公开(公告)号：US20110107581A1
公开(公告)日：2011-05-12
申请号：US12940909
申请日：2010-11-05
申请人： David Delon Williams , William G. Pitt , Peter Austin Jepsen , Douglas Turley Wright , Carl Jay Ellingson , Daniel August Susumu Marler
发明人： David Delon Williams , William G. Pitt , Peter Austin Jepsen , Douglas Turley Wright , Carl Jay Ellingson , Daniel August Susumu Marler
IPC分类号： B23P11/00 , B29C67/00 , B29C37/00
CPC分类号： B29C57/00 , A61F2/5044 , A61F2/66 , A61F2/80 , A61F2002/5003 , A61F2002/5056 , A61F2002/5072 , A61F2002/6621 , A61F2002/665 , A61F2002/6664 , B29C53/02 , B29C2793/0081 , B29L2031/7532 , Y10T29/49789 , Y10T29/49798 , Y10T29/49826 , Y10T29/49865 , Y10T29/49947 , Y10T29/49948 , Y10T29/4998
摘要： The present invention provides divided-end artificial limbs and methods for making divided-end artificial limbs that that are inexpensive, simple, and customizable. A limb is formed by dividing an end of an elongated shaft member into extending prongs that are shaped into custom lengths and curvatures. Furthermore, the method of the present invention provides affixing a sole plate to the limb, reinforcement of the prongs by nesting additional prongs within the elongated shaft member, dividing the proximal end of the shaft member into circumferentially spaced-apart fingers, and dividing a midsection of the shaft member into circumferentially spaced-apart flexing vanes. The present invention also provides that the prongs may be further modified by division into toe and heel extensions or by wrapping a prong around another prong to form a sole. Methods of reducing stress concentration using bolts and stress relief holes in these limbs are also presented.
摘要翻译：本发明提供了分割端假肢及其制造方法，其是廉价，简单且可定制的分割假肢。通过将细长轴构件的端部分成成为定制长度和曲率的延伸尖端而形成肢体。此外，本发明的方法提供了将底板固定在肢体上，通过在细长轴构件内嵌入附加插脚来加强插脚，将轴构件的近端分成周向间隔开的手指，并将中间部分的轴构件形成周向间隔开的挠曲叶片。本发明还提供了，可以通过分割成脚趾和脚跟延伸部或通过围绕另一个插脚包裹叉子来进一步修改插脚以形成鞋底。还提出了使用这些肢体中的螺栓和应力释放孔来减少应力集中的方法。

8. 发明授权

US5696312A Accelerated impact testing apparatus 失效
标题翻译：加速冲击试验装置
公开(公告)号：US5696312A
公开(公告)日：1997-12-09
申请号：US556584
申请日：1995-11-13
申请人： Nathan J. Lee , William G. Pitt
发明人： Nathan J. Lee , William G. Pitt
IPC分类号： G01N3/48 , G01N3/52 , G01N33/00 , G01N3/00
CPC分类号： G01N3/48 , G01N3/52 , G01N2033/008
摘要： Accelerated impact testing apparatus which simulates the dynamic strain in golf clubs, shafts and other specimens by rapidly transferring kinetic energy to the specimen from an instrumented tup that is free to rebound. The specimen is held in place by two holding fixtures uniquely designed to provide the appropriate boundary and support conditions for the transient strain to propagate in a manner similar to that of the actual application e.g. a golf club striking a ball. Force applied to the tup is generated by compressing a spring to a predetermined length and releasing its energy through a rapidly actuated latch. Actual force applied as a function of time is measured by a load cell located in the tup and the tup velocity just prior to impact is computed from a velocity sensor signal. Acceleration of the impact event is also recorded as well as strain levels in the shaft.
摘要翻译：加速冲击试验装置，通过从能够自由反弹的仪表托盘快速将动能转移到试样上，模拟高尔夫球杆，轴和其他试样中的动态应变。样品通过两个固定装置固定在适当的位置，其独特地设计成为瞬时应变提供适当的边界和支撑条件，以与实际应用类似的方式传播。一个打高尔夫球的球。通过将弹簧压缩到预定长度并通过快速致动的闩锁释放其能量来产生施加到托盘上的力。作为时间的函数施加的实际力由位于tup中的测力传感器测量，并且刚好在冲击之前的速度传感器信号由速度传感器信号计算。还记录了冲击事件的加速度以及轴中的应变水平。

9. 发明授权

US5112736A DNA sequencing using fluorescence background electroblotting membrane 失效
标题翻译： DNA序列使用荧光背景电泳膜
公开(公告)号：US5112736A
公开(公告)日：1992-05-12
申请号：US365693
申请日：1989-06-14
申请人： Karin D. Caldwell , Tun-Jen Chu , William G. Pitt
发明人： Karin D. Caldwell , Tun-Jen Chu , William G. Pitt
IPC分类号： B01D67/00 , C12Q1/68
CPC分类号： B01D67/0093 , C12Q1/6869 , C12Q1/6874 , B01D2323/30 , Y10S435/805 , Y10S436/80 , Y10S436/807 , Y10T436/175383
摘要： A method for the multiplex sequencing on DNA is disclosed which comprises the electroblotting or specific base terminated DNA fragments, which have been resolved by gel electrophoresis, onto the surface of a neutral non-aromatic polymeric microporous membrane exhibiting low background fluorescence which has been surface modified to contain amino groups. Polypropylene membranes are preferably and the introduction of amino groups is accomplished by subjecting the membrane to radio or microwave frequency plasma discharge in the presence of an aminating agent, preferably ammonia. The membrane, containing physically adsorbed DNA fragments on its surface after the electroblotting, is then treated with crosslinking means such as UV radiation or a glutaraldehyde spray to chemically bind the DNA fragments to the membrane through said smino groups contained on the surface thereof. The DNA fragments chemically bound to the membrane are subjected to hybridization probing with a tagged probe specific to the sequence of the DNA fragments. The tagging may be by either fluorophores or radioisotopes. The tagged probes hybridized to said target DNA fragments are detected and read by laser induced fluorescence detection or autoradiograms. The use of aminated low fluorescent background membranes allows the use of fluorescent detection and reading even when the available amount of DNA to be sequenced is small. The DNA bound to the membrances may be reprobed numerous times.
摘要翻译：公开了一种用于DNA上多重测序的方法，该方法包括已经通过凝胶电泳解析的电印迹或特异性碱基封端的DNA片段到已经表面改性的显示低背景荧光的中性非芳族聚合物微孔膜的表面上含有氨基。聚丙烯膜是优选的，氨基的引入是通过在胺化剂，优选氨的存在下使膜进行无线电或微波频率等离子体放电来实现的。然后在电印迹之后在其表面上含有物理吸附的DNA片段的膜用诸如UV辐射或戊二醛喷雾剂的交联手段进行处理，以通过其表面上包含的所述唾液酸基将DNA片段化学结合到膜上。用与DNA片段序列特异的标记探针对化学键合到膜上的DNA片段进行杂交探测。标记可以是荧光团或放射性同位素。通过激光诱导荧光检测或放射自显影检测和读取与所述靶DNA片段杂交的标记探针。使用胺化的低荧光背景膜允许使用荧光检测和阅读，即使待测DNA的可用量很小。与膜结合的DNA可以重复多次。

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