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1. 发明授权

US5604114A Cis-acting repression sequences, cis-acting antirepression sequences, vectors, methods of preparation and use 失效
标题翻译：顺式作用抑制序列，顺式作用抗逆序列，载体，制备和使用方法
公开(公告)号：US5604114A
公开(公告)日：1997-02-18
申请号：US41887
申请日：1993-04-02
申请人： William A. Haseltine , Craig A. Rosen , Joseph G. Sodroski , Ernest Terwilliger , Wei C. Goh
发明人： William A. Haseltine , Craig A. Rosen , Joseph G. Sodroski , Ernest Terwilliger , Wei C. Goh
IPC分类号： C07K14/16 , C07K14/61 , C12N15/11 , C12N15/85 , C12N15/49 , C12N15/63
CPC分类号： C07K14/005 , C07K14/61 , C12N15/11 , C12N15/85 , C12N2740/16022 , C12N2740/16322
摘要： Cis-acting repression sequences which are able to provide a cis-acting inhibitory effect on the expression of a gene when placed downstream of the gene in its untranslated message are dislcosed. Cis-acting anti-repression sequences which can relieve the cis-acting repression in the presence of the art gene product are also disclosed. These sequences correspond to a sufficient number of nucleotides from the HIV-I, HIV-2, STLV-3 or HTLV-IV genomes to provide the repression or anti-repression effects. The use of the sequences in vectors and systems to control the expression of a desired gene product is also described.
摘要翻译：当其位于其未翻译消息的下游位置时，能够提供对基因表达的顺式作用抑制作用的顺式作用抑制序列被消除。还公开了可以在现有基因产物存在下缓解顺式作用抑制的顺式作用的抗抑制序列。这些序列对应于来自HIV-1，HIV-2，STLV-3或HTLV-IV基因组的足够数量的核苷酸以提供抑制或抗抑制作用。还描述了使用载体和系统中的序列来控制所需基因产物的表达。

2. 发明授权

US4981790A Stable TatIII cell lines, TatIII gene products, and assay methods 失效
公开(公告)号：US4981790A
公开(公告)日：1991-01-01
申请号：US806263
申请日：1985-12-06
申请人： William A. Haseltine , Craig A. Rosen , Joseph G. Sodroski , Wei C. Goh
发明人： William A. Haseltine , Craig A. Rosen , Joseph G. Sodroski , Wei C. Goh
IPC分类号： C12P19/34 , C07K14/15 , C07K14/16 , C12N5/10 , C12N15/00 , C12N15/09 , C12N15/85 , C12P21/00 , C12R1/91
CPC分类号： C07K14/005 , C12N15/85 , C12N2740/14022 , C12N2740/16322
摘要： This invention describes stable tat.sub.III cell lines. It is disclosed that by transfecting a preselected tat.sub.III cell line with a vector containing a sufficient amount of the HTLV-III LTR responsive to tat.sub.III gene products for trans-activation and an enhancer upstream of the tat.sub.III responsive segment, it is possible to express high levels of the tat.sub.III gene products. By including a preselected heterologous gene on this vector, it is also possible to express high levels of a desired gene product. A substantially pure protein comprising 86 amino acids and having an apparent molecular weight of about 14,000 dalton and exhibiting trans-activating activity is also disclosed. This protein and polypeptides having trans-activating ability, which is also disclosed, can be used to produce high levels of a desired gene product. A method of detecting the presence of HTLV-III/LAV virus in an individual is also disclosed and comprises the step of:(a) incubating whole blood or lymphocytes from the invididual to be tested with tat.sub.III cell lines of the present invention in a culture medium; and(b) screening for cytopathic effects on the cells is also disclosed. A method of screening for a compound that inhibits trans-activation of the tat.sub.III gene product is also disclosed and comprises the steps of:(1) transacting a tat.sub.III cell line of the present invention with a vector containing a gene that expresses a selectable marker and whose expression is under the control of an HTLV-III LTR;(2) transfecting the same type of tat.sub.III cell lines as in step (1) with the selectable gene chosen in step (1) but under the control of a different regulatory sequence;(3) thereafter, adding a preselected compound to each of the cell lines in increasing concentrations; and(4) measuring the expression of the selectable gene product to determine whether the compound effects the tat.sub.III function without being toxic to the cell.

3. 发明授权

US5321124A Art (rev) protein of human T-cell leukemia virus 失效
标题翻译：人类T细胞白血病病毒的Art（rev）蛋白
公开(公告)号：US5321124A
公开(公告)日：1994-06-14
申请号：US995948
申请日：1992-12-18
申请人： William A. Haseltine , Craig A. Rosen , Joseph G. Sodroski , Wei C. Goh
发明人： William A. Haseltine , Craig A. Rosen , Joseph G. Sodroski , Wei C. Goh
IPC分类号： C12N15/00 , A61K39/12 , C07K1/22 , C07K14/00 , C07K14/155 , C07K14/16 , C12N5/00 , C12N15/85 , C12P21/02 , C12R1/91 , C07K13/00
CPC分类号： C07K14/005 , C12N15/85 , C12N2740/14022 , C12N2740/16322 , Y10S930/221
摘要： A gene and gene product that regulates the expression of the capsidal envelope genes of HTLV-III/LAV and that can be used to regulate the expression of heterologous (non-viral) genes as well is disclosed. This art gene consists of two exons and can be used in creating nucleotide segments, vectors and cell lines. A new method for screening for compounds that inhibit the replication of HTLV-III is also described and comprises:(1) transfecting a T-cell line with the HTLV-III art and env genes;(2) thereafter, adding a preselected compound to the transformed cell line in increasing concentrations; and(3) determining whether the compound effects the art function without being toxic to the cell.An additional parameter to use in diagnosis of AIDS disease is also described. The use of the art gene and gene product in AIDS therapy is also disclosed.
摘要翻译：公开了一种调节HTLV-III / LAV的包膜基因表达并可用于调节异源（非病毒）基因表达的基因和基因产物。该艺术基因由两个外显子组成，可用于产生核苷酸片段，载体和细胞系。还描述了用于筛选抑制HTLV-III复制的化合物的新方法，其包括：（1）用HTLV-III技术和env基因转染T细胞系; （2）之后，以增加的浓度向转化细胞系中加入预选化合物; 和（3）确定化合物是否影响艺术功能而不对细胞有毒性。还描述了用于诊断AIDS疾病的另外的参数。还公开了在艾滋病治疗中使用艺术基因和基因产物。

4. 发明授权

US4935372A Art nucleotide segments, vectors, cell lines methods of preparation and use 失效
标题翻译：艺术核苷酸片段，载体，细胞系的制备和使用方法
公开(公告)号：US4935372A
公开(公告)日：1990-06-19
申请号：US865151
申请日：1986-05-20
申请人： Wei C. Goh
发明人： Wei C. Goh
IPC分类号： C12N15/00 , A61K39/12 , C07K1/22 , C07K14/00 , C07K14/155 , C07K14/16 , C12N5/00 , C12N15/85 , C12P21/02 , C12R1/91
CPC分类号： C07K14/005 , C12N15/85 , C12N2740/14022 , C12N2740/16322 , Y10S930/221
摘要： A gene and gene product that regulates the expression of the capsidal envelope genes of HTLV-III/LAV and that can be used to regulate the expression of heterologous (non-viral) genes as well is disclosed. This art gene consists of two exons and can be used in creating nucleotide segments, vectors and cell lines. A new method for screening for compounds that inhibit the replication of HTLV-III is also described and comprises:(1) transfecting a T-cell line with the HTLV-III art and env genes;(2) thereafter, adding a preselected compound to the transformed cell line in increasing concentrations; and(3) determining whether the compound effects the art function without being toxic to the cell.An additional parameter to use in diagnosis of AIDS disease is also described. The use of the art gene and gene product in AIDS therapy is also disclosed.
摘要翻译：公开了一种调节HTLV-III / LAV的包膜基因表达并可用于调节异源（非病毒）基因表达的基因和基因产物。该艺术基因由两个外显子组成，可用于产生核苷酸片段，载体和细胞系。还描述了用于筛选抑制HTLV-III复制的化合物的新方法，其包括：（1）用HTLV-III技术和env基因转染T细胞系; （2）之后，以增加的浓度向转化细胞系中加入预选化合物; 和（3）确定化合物是否影响艺术功能而不对细胞有毒性。还描述了用于诊断AIDS疾病的另外的参数。还公开了在艾滋病治疗中使用艺术基因和基因产物。

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