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1. 发明申请

US20060134084A1 Therapeutic platelets and methods 审中-公开
公开(公告)号：US20060134084A1
公开(公告)日：2006-06-22
申请号：US11317411
申请日：2005-12-22
申请人： Willem Wolkers , John Crowe , Fern Tablin , Ann Oliver , Naomi Walker
发明人： Willem Wolkers , John Crowe , Fern Tablin , Ann Oliver , Naomi Walker
IPC分类号： A61K35/14 , A01N1/02 , C12N5/08
CPC分类号： A61K35/19 , A01N1/0221 , A01N1/0231 , A01N1/0284 , A61K45/06 , A61K47/6901 , Y10T436/108331 , A61K2300/00
摘要： A dehydrated composition is provided that includes freeze-dried platelets. The platelets are loaded with trehalose which preserves biological properties during freeze-drying and rehydration. The trehalose loading is conducted at a temperature of from greater than about 25° C. to less than about 40° C., most preferably at 37° C., with the loading solution having trehalose in an amount from about 10 mM to about 50 mM. These freeze-dried platelets are substantially shelf-stable and are rehydratable so as to have a normal response to an agonist, for example, thrombin, with virtually all of the platelets participating in clot formation within about three minutes at 37° C.

2. 发明申请

US20060223050A1 Therapeutic platelets and methods 审中-公开
标题翻译：治疗性血小板和方法
公开(公告)号：US20060223050A1
公开(公告)日：2006-10-05
申请号：US10567593
申请日：2004-08-06
申请人： John Crowe , Fern Tablin , Willem Wolkers , Naomi Walker , Joong-Hyuck Auh , Minke Tang , Sheri Looper
发明人： John Crowe , Fern Tablin , Willem Wolkers , Naomi Walker , Joong-Hyuck Auh , Minke Tang , Sheri Looper
IPC分类号： A01N1/02 , C12N5/08 , C12N5/00
CPC分类号： A01N1/0221 , A01N1/02
摘要： The invention provides methods for loading a preservative into blood platelets comprising providing a preservative solution having a preservative, water and protein, and loading blood platelets with the preservative solution to produce preservative-loaded blood platelets having the preservative solution generally including higher glass transition temperatures than glass transition temperatures for a preservative solution having the preservative, water and no protein. A process for processing blood platelets comprising suspending blood platelets in a preservative solution at a concentration greater than about 108 platelets per ml. of preservative solution to produce preservative-loaded blood platelets, freeze-drying the preservative-loaded blood platelets, and recovering at least 75% of the freeze died platelets. A platelet composition comprising blood platelets loaded with a preservative solution having a preservative, water, and protein, and generally further having higher glass transition temperatures than glass transition temperatures for blood platelets loaded with the preservative, water, but no protein.
摘要翻译：本发明提供了将防腐剂装载到血小板中的方法，其包括提供具有防腐剂，水和蛋白质的防腐剂溶液，并将血小板与防腐剂溶液加载以产生具有防腐剂溶液的血小板，所述血小板具有通常包含较高玻璃化转变温度具有防腐剂，水和无蛋白质的防腐溶液的玻璃化转变温度。一种处理血小板的方法，包括以大于约108个血小板/ ml的浓度将血小板悬浮在防腐溶液中。的防腐溶液以产生防腐剂血小板，冷冻干燥载有防腐剂的血小板，并回收至少75％的冷冻死亡血小板。一种血小板组合物，其包含负载有防腐剂，水和蛋白质的防腐溶液的血小板，并且通常进一步具有比装有防腐剂，水但不含蛋白质的血小板的玻璃化转变温度更高的玻璃化转变温度。

3. 发明申请

US20070026377A1 Methods for preserving nucleated mammalian cells 审中-公开
标题翻译：保存有核哺乳动物细胞的方法
公开(公告)号：US20070026377A1
公开(公告)日：2007-02-01
申请号：US10575832
申请日：2004-10-18
申请人： John Crowe , Fern Tablin , Ann Oliver , Kamran Jamil , Xiaocui Ma , James Clegg , Willem Wolkers , Thurein Htoo
发明人： John Crowe , Fern Tablin , Ann Oliver , Kamran Jamil , Xiaocui Ma , James Clegg , Willem Wolkers , Thurein Htoo
IPC分类号： A01N1/02 , A61K31/7012
CPC分类号： A01N1/02 , A01N1/0221 , A61K31/7012
摘要： Methods and compositions are provided for increasing the survival of nucleated mammalian cells following drying and rehydration. The methods include introducing a disaccharide such as trehalose into said cells, optionally including heat shock proteins, apoptosis inhibitors, and arbutin, drying said cells, and rehydrating them. The invention further provides nucleated mammalian cells that have increased capacity to survive, divide and, in some cases, differentiate, following drying and rehydration. The cells comprise a disaccharide and one or more of the following: a heat shock protein, an apoptosis inhibitor, and arbutin.
摘要翻译：提供了用于在干燥和补液后增加有核哺乳动物细胞的存活的方法和组合物。所述方法包括将二糖如海藻糖引入所述细胞中，任选地包括热休克蛋白，凋亡抑制剂和熊果苷，干燥所述细胞并使其再水合。本发明进一步提供成核的哺乳动物细胞，其在干燥和补液后具有增加的存活能力，分裂能力以及在某些情况下区分。细胞包含二糖和一种或多种以下物质：热休克蛋白，凋亡抑制剂和熊果苷。

4. 发明申请

US20060134069A1 Erythrocytic cells and method for preserving cells 审中-公开
公开(公告)号：US20060134069A1
公开(公告)日：2006-06-22
申请号：US10889935
申请日：2004-07-12
申请人： John Crowe , Lois Crowe , Fern Tablin , Willem Wolkers , Nelly Tsvetkova , Ann Oliver
发明人： John Crowe , Lois Crowe , Fern Tablin , Willem Wolkers , Nelly Tsvetkova , Ann Oliver
IPC分类号： A61K35/14
CPC分类号： A01N1/0205 , A01N1/02 , A01N1/0221 , A01N1/0226 , A61K35/18 , A61K47/6901 , C12N5/0641
摘要： A dehydrated composition is provided that includes freeze-dried erythrocytic cells. Alcohol (e.g., sterol or cholesterol) is at least partially removed from erythrocytic cells including erythrocytic membranes. After removal of at least part of the alcohol, the erythrocytic cells have a low phase transition temperature range, an intermediate phase transition temperature range, and a high phase transition temperature range. The erythrocytic cells may be loaded with an oligosaccharide (e.g., trehalose) which preserves biological properties during freeze-drying and rehydration. A process for increasing cooperativity of a phase transition of an erythrocytic cell. A process for preserving and/or increasing the survival of dehydrated erythrocytic cells, including storing dehydrated erythrocytic cells having a residual water content equal to or less than about 0.30 gram of water per gram of dry weight erythrocytic cells.

5. 发明申请

US20050112686A1 Method for introducing molecules into biological samples 审中-公开
标题翻译：将分子引入生物样品的方法
公开(公告)号：US20050112686A1
公开(公告)日：2005-05-26
申请号：US10722154
申请日：2003-11-25
申请人： John Crowe , Fern Tablin , Willem Wolkers , Ann Oliver , Kamran Jamil , Joong-Hyuck Auh , Thurein Htoo
发明人： John Crowe , Fern Tablin , Willem Wolkers , Ann Oliver , Kamran Jamil , Joong-Hyuck Auh , Thurein Htoo
IPC分类号： C12Q1/34 , G01N33/53
CPC分类号： A01N1/0221
摘要： A method for loading a biological sample comprising loading a biological sample with a solute by fluid phase endocytosis to produce an internally loaded biological sample. Within the biological sample a first matter (e.g., a vesicle) having the solute fuses with a second matter (e.g., a lysosome) to produce a fused matter containing the solute. Loading of the biological sample includes transferring the solute from the fused matter into cytoplasm within the biological sample.
摘要翻译：一种用于装载生物样品的方法，包括通过流体相内吞作用加载具有溶质的生物样品以产生内部负载的生物样品。在生物样品中，具有溶质的第一物质（例如囊泡）与第二物质（例如，溶酶体）融合以产生含有溶质的熔融物质。生物样品的载体包括将溶质从融合物转移到生物样品内的细胞质中。

6. 发明申请

US20050048460A1 Preservative and method for preserving cells 审中-公开
标题翻译：防腐剂和保存细胞的方法
公开(公告)号：US20050048460A1
公开(公告)日：2005-03-03
申请号：US10853062
申请日：2004-05-24
申请人： John Crowe , Fern Tablin , Ann Oliver , Kamran Jamil
发明人： John Crowe , Fern Tablin , Ann Oliver , Kamran Jamil
IPC分类号： A01N1/00 , A01N1/02 , A01N43/04 , A01N65/00 , A61K31/70 , C12N20060101
CPC分类号： C12N9/96 , A01N1/0221
摘要： A method for stabilizing a biological material (e.g., blood platelets, cells, etc.) comprising treating a biological material with an amphiphilic agent (e.g., an amphiphilic compound, such as a surfactant, or pluronic or arbutin) to stabilize the biological material. At least one carbohydrate (e.g., trehalose or a trehalose-sucrose mixture) may be combined with the amphiphilic agent for treating the biological material. The treated biological material may be dehydrated. A biological material produced in accordance with the method for treating the biological material.
摘要翻译：一种用于稳定生物材料（例如，血小板，细胞等）的方法，包括用两亲性试剂（例如两亲性化合物，例如表面活性剂或普朗尼克或熊果苷）处理生物材料以稳定生物材料。至少一种碳水化合物（例如海藻糖或海藻糖 - 蔗糖混合物）可与两亲性试剂组合以处理生物材料。经处理的生物材料可以脱水。根据生物材料处理方法生产的生物材料。

7. 发明申请

US20050032031A1 Method for eliminating fragile cells from stored cells 审中-公开
标题翻译：从存储的细胞中消除脆弱细胞的方法
公开(公告)号：US20050032031A1
公开(公告)日：2005-02-10
申请号：US10635754
申请日：2003-08-06
申请人： John Crowe , Fern Tablin , Nelly Tsvetkova , Zsolt Torok , Gyana Satpathy , Denis Dwyre , Rachna Bali
发明人： John Crowe , Fern Tablin , Nelly Tsvetkova , Zsolt Torok , Gyana Satpathy , Denis Dwyre , Rachna Bali
IPC分类号： A01N1/02 , C12N5/08
CPC分类号： A01N1/0221 , A01N1/02
摘要： A method for reducing hemolysis in cells including washing cells in a solute solution having the capabilities of reducing cell hemolysis by at least about 0.50% for each 100 mOsm increase in osmolarity of the solute solution. A cell produced by the method for reducing hemolysis. The method permits removal of osmotically fragile cells from the population.
摘要翻译：一种减少细胞溶血的方法，包括在溶质溶液中的洗涤细胞，其具有将溶质溶液的渗透压增加的每100mOsm减少细胞溶血至少约0.50％的能力。通过减少溶血的方法生产的细胞。该方法允许从群体中除去渗透性脆弱的细胞。

8. 发明申请

US20050031596A1 Cells and method for preserving cells 审中-公开
标题翻译：细胞和保存细胞的方法
公开(公告)号：US20050031596A1
公开(公告)日：2005-02-10
申请号：US10635795
申请日：2003-08-06
申请人： John Crowe , Fern Tablin , Nelly Tsvetkova , Zsolt Torok , Rachna Bali , Gyana Satpathy , Denis Dwyre
发明人： John Crowe , Fern Tablin , Nelly Tsvetkova , Zsolt Torok , Rachna Bali , Gyana Satpathy , Denis Dwyre
IPC分类号： A01N1/02 , A61K31/7012 , A61K45/06 , A61K45/00
CPC分类号： A01N1/02 , A01N1/0221 , A61K31/7012 , A61K45/06
摘要： A dehydrated composition is provided that includes freeze-dried cells. A method for loading a solute into a cell comprising disposing a cell in a solution having at temperature of at least 25° C. and a solute concentration of sufficient magnitude to produce hyperosmotic pressure on the cell for transferring a solute from the solution into the cell. A method for reconstituting dried cells by drying loaded cells in a drying solution and reconstituting dried cells in a rehydration solution. A method for stabilizing cells by producing cells having an effective amount of a solute and a mean corpuscular hemoglobin greater than about 10.
摘要翻译：提供了包含冷冻干燥细胞的脱水组合物。一种用于将溶质装入细胞的方法，包括将细胞置于具有至少25℃的温度和足够大的溶质浓度的溶液中，以在细胞上产生高渗压以将溶质从溶液转移到细胞中。通过干燥干燥溶液中负载的细胞并在再水化溶液中重构干燥的细胞来重构干细胞的方法。通过产生具有大于约10的有效量的溶质和平均红细胞血红蛋白的细胞来稳定细胞的方法。

9. 发明申请

US20050051474A1 Erythrocytic cells and method for loading solutes 审中-公开
标题翻译：红细胞和负载溶质的方法
公开(公告)号：US20050051474A1
公开(公告)日：2005-03-10
申请号：US10635396
申请日：2003-08-06
申请人： John Crowe , Fern Tablin , Nelly Tsvetkova , Zsolt Torok , Gyana Satpathy , Denis Dwyre
发明人： John Crowe , Fern Tablin , Nelly Tsvetkova , Zsolt Torok , Gyana Satpathy , Denis Dwyre
IPC分类号： A01N1/02 , B01D11/00 , C12N5/078
CPC分类号： A01N1/02 , A01N1/0221 , A01N1/0226 , C12N5/0641
摘要： A dehydrated composition is provided that includes freeze-dried erythrocytic cells. A method for loading a solute into a cell comprising disposing a cell in a solution having a solute concentration of sufficient magnitude to produce hyperosmotic pressure on the cell for transferring a solute from the solution into the cell. A method for retaining a solute in a cell.
摘要翻译：提供脱水组合物，其包括冷冻干燥的红血球细胞。一种将溶质负载到细胞中的方法，包括将细胞置于具有足够量级的溶质浓度的溶液中以在细胞上产生高渗渗压，以将溶质从溶液转移到细胞中。一种将溶质保留在电池中的方法。

10. 发明申请

US20050031597A1 Cells and improved method for preserving cells 审中-公开
标题翻译：细胞和改良的保存细胞的方法
公开(公告)号：US20050031597A1
公开(公告)日：2005-02-10
申请号：US10724372
申请日：2003-11-28
申请人： John Crowe , Fern Tablin , Nelly Tsvetkova , Zsolt Torok , Gyana Satpathy , Rachna Bali , Denis Dwyre
发明人： John Crowe , Fern Tablin , Nelly Tsvetkova , Zsolt Torok , Gyana Satpathy , Rachna Bali , Denis Dwyre
IPC分类号： A01N1/02 , A61K31/7012 , A61K45/06 , A61K45/00 , A61K31/045 , A61K31/70
CPC分类号： A01N1/02 , A01N1/0221
摘要： A method for maintaining a viability level of a metabolite in a biological sample comprising disposing a biological sample into a solute solution comprising a solute and a chemical selected from the group consisting of a monosaccharide, a monosaccharide polyol, a cell metabolite-controlling agent, a salt, and mixtures thereof; and incubating the biological sample in the solute solution while maintaining a viability level of a metabolite in the biological sample.
摘要翻译：一种用于维持生物样品中代谢物生存能力水平的方法，包括将生物样品置于包含溶质和选自单糖，单糖多元醇，细胞代谢控制剂，盐及其混合物; 并将生物样品在溶质溶液中孵育，同时保持生物样品中代谢物的活力水平。

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