专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US07250251B2 Virion-based fusion assay 有权
标题翻译：基于Virion的融合测定
公开(公告)号：US07250251B2
公开(公告)日：2007-07-31
申请号：US10656803
申请日：2003-09-04
申请人： Warner C. Greene , Marielle Cavrois , Carlos de Noronha
发明人： Warner C. Greene , Marielle Cavrois , Carlos de Noronha
IPC分类号： C12Q1/70
CPC分类号： C07K14/005 , C07K2319/00 , C12N7/00 , C12N2740/16322 , C12Q1/6897
摘要： The present invention features methods and compositions relating to a virion-based fusion assay for detection of infection of a target cell by an enveloped retroviral virion such as HIV. The assay uses virions containing a chimeric viral protein comprising a viral accessory polypeptide (such as Vpr) fused to a reporter polypeptide (such as beta-lactamase). Fusion of the virion with a target cell membrane results in intracellular delivery of the chimeric protein to the target cell, which in turn provides for detection of a detectable signal mediated by the reporter polypeptide portion of the chimeric polypeptide. Significant detectable signal is only detected following intracellular delivery of the chimeric viral protein, thus providing for detection of productive viral entry to the exclusion of non-productive, endocytic entry of virions into the cell.
摘要翻译：本发明的特征在于与基于病毒体的融合测定相关的方法和组合物，用于通过包膜的逆转录病毒药物（例如HIV）检测靶细胞的感染。该测定使用含有嵌合病毒蛋白质的病毒体，其包含与报道多肽（例如β-内酰胺酶）融合的病毒辅助多肽（例如Vpr）。病毒粒子与靶细胞膜的融合导致嵌合蛋白质的细胞内递送至靶细胞，其又提供检测由嵌合多肽的报道多肽部分介导的可检测信号。仅在嵌合病毒蛋白质的细胞内递送后才检测到显着的可检测信号，从而提供检测生产性病毒进入以排除病毒体进入细胞的非生产性内吞进入。

IPRDB

热门服务

关于我们

友情链接

联系方式