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1. 发明授权

US5401642A Vectors and methods for making such vectors and for expressing cloned genes 失效
标题翻译：用于制备这种载体和表达克隆基因的载体和方法
公开(公告)号：US5401642A
公开(公告)日：1995-03-28
申请号：US59193
申请日：1993-05-07
申请人： Walter C. Fiers , Erik R. Remaut
发明人： Walter C. Fiers , Erik R. Remaut
IPC分类号： A61K39/135 , C07K14/025 , C07K14/565 , C12N1/21 , C12N15/52 , C12P21/00 , C12P21/02
CPC分类号： C07K14/005 , A61K39/12 , A61K39/135 , C07K14/565 , C12N15/52 , C12N2710/22022 , C12N2770/32134
摘要： Improved vectors and methods for expressing cloned genes of prokaryotic or eukaryotic origin and methods of making such vectors are disclosed, the improved vectors comprising promoters and operators from .lambda. phages and preferably do not include an active cro gene or an active N gene, the vectors having at least one endonuclease recognition site for cloning desired genes less than about 300 base pairs from the promoters and operators and being useful, as are methods utilizing the vectors, in producing a wide variety of prokaryotic, eukaryotic and vital polypeptides, hormones, enzymes, antigens, proteins and amino acids.
摘要翻译：公开了用于表达原核或真核来源的克隆基因的改进的载体和方法以及制备此类载体的方法，改进的载体包含λ噬菌体的启动子和操纵子，优选不包括活性的克隆基因或活性N基因，所述载体具有至少一个用于克隆来自启动子和操纵子的小于约300个碱基对的所需基因的内切核酸酶识别位点，并且如使用该载体的方法在生产多种原核，真核和重要多肽，激素，酶，抗原，蛋白质和氨基酸。

2. 发明授权

US5401658A Vectors and methods for making such vectors and for expressing cloned genes 失效
公开(公告)号：US5401658A
公开(公告)日：1995-03-28
申请号：US753739
申请日：1991-09-03
申请人： Walter C. Fiers , Erik R. Remaut
发明人： Walter C. Fiers , Erik R. Remaut
IPC分类号： A61K39/135 , C07K14/025 , C07K14/565 , C12N1/21 , C12N15/52 , C12N15/00 , C12N15/73
CPC分类号： C07K14/005 , A61K39/12 , A61K39/135 , C07K14/565 , C12N15/52 , C12N2710/22022 , C12N2770/32134
摘要： Improved vectors and methods for expressing cloned genes of prokaryotic or eukaryotic origin and methods of making such vectors are disclosed, the improved vectors comprising promoters and operators from .lambda. phages and preferably do not include an active cro gene or an active N gene, the vectors having at least one endonuclease recognition site for cloning desired genes less than about 300 base pairs from the promoters and operators and being useful, as are methods utilizing the vectors, in producing a wide variety of prokaryotic, eukaryotic and vital polypeptides, hormones, enzymes, antigens, proteins and amino acids.

3. 发明授权

US5004689A DNA sequences, recombinant DNA molecules and processes for producing human gamma interferon-like polypeptides in high yields 失效
标题翻译： DNA序列，重组DNA分子和以高产率产生人γ干扰素样多肽的方法
公开(公告)号：US5004689A
公开(公告)日：1991-04-02
申请号：US31042
申请日：1987-03-27
申请人： Walter C. Fiers , Bernard Allet
发明人： Walter C. Fiers , Bernard Allet
IPC分类号： A61K38/00 , C07K14/57 , C12N1/21 , C12N15/23 , C12N15/67 , C12N15/70 , C12N15/85
CPC分类号： C12N15/67 , C07K14/57 , C12N15/70 , C12N15/85 , A61K38/00
摘要： DNA sequences, recombinant DNA molecules and hosts transformed with them which produce polypeptides displaying a biological or immunological activity of gamma interferon. The genes coding for these polypeptides and methods of making and using these DNA sequences, molecules, hosts, genes and polypeptides are disclosed. The DNA sequences of this invention are further characterized by expression control sequences which permit the production of gamma interferon in high yields. More particularly, these expression control sequences comprise the .lambda. P.sub.L promoter, and more preferably, a trp-derived expression control sequence containing the sequence ATCGATACT between the Shine-Dalgarno sequence and the translational start signal. In appropriate hosts, these DNA sequences and recombinant DNA molecules permit the production and identification of genes and polypeptides displaying a biological or immunological activity of gamma interferon and their use in antiviral, antitumor or anticancer, and immunomodulation agents and methods.
摘要翻译： DNA序列，重组DNA分子和用它们转化的宿主，其产生显示γ干扰素的生物学或免疫学活性的多肽。公开了编码这些多肽的基因以及制备和使用这些DNA序列，分子，宿主，基因和多肽的方法。本发明的DNA序列的进一步特征在于允许以高产率生产γ干扰素的表达控制序列。更具体地说，这些表达控制序列包括λPL启动子，更优选地，含有Shine-Dalgarno序列和翻译起始信号之间的序列ATCGATACT的trp衍生的表达控制序列。在适当的宿主中，这些DNA序列和重组DNA分子允许产生和鉴定表现出γ干扰素的生物学或免疫学活性的基因和多肽及其在抗病毒，抗肿瘤或抗癌剂中的用途以及免疫调节剂和方法。

4. 发明授权

US4874702A Vectors and methods for making such vectors and for expressive cloned genes 失效
标题翻译：用于制备这种载体和表达克隆基因的载体和方法
公开(公告)号：US4874702A
公开(公告)日：1989-10-17
申请号：US921803
申请日：1986-10-20
申请人： Walter C. Fiers , Rene Erik Remaut
发明人： Walter C. Fiers , Rene Erik Remaut
IPC分类号： A61K39/135 , C07K14/025 , C07K14/565 , C12N1/21 , C12N15/52
CPC分类号： C07K14/005 , A61K39/12 , A61K39/135 , C07K14/565 , C12N15/52 , C12N2710/22022 , C12N2770/32134
摘要： Improved vectors and methods for expressing cloned genes of prokaryotic or eukaryotic origin and methods of making such vectors are disclosed, the improved vectors comprising promoters and operators from .lambda. phages and preferably do not include an active cro gene or an active N gene, the vectors having at least one endonuclease recognition site for cloning desired genes less than about 300 base pairs from the promoters and operators and being useful, as are methods utilizing the vectors, in producing a wide variety of prokaryotic, eukaryotic and viral polypeptides, hormones, enzymes, antigens, proteins and amino acids.
摘要翻译：公开了用于表达原核或真核来源的克隆基因的改进的载体和方法以及制备此类载体的方法，改进的载体包含λ噬菌体的启动子和操纵子，优选不包括活性的克隆基因或活性N基因，所述载体具有至少一个用于克隆来自启动子和操纵子的小于约300个碱基对的所需基因的内切核酸酶识别位点，并且如使用该载体的方法在生产多种原核，真核和病毒多肽，激素，酶，抗原，蛋白质和氨基酸。

5. 发明授权

US4980160A Combinations of tumor necrosis factors and anti-inflammatory agents and methods for treating malignant and non-malignant diseases 失效
标题翻译：肿瘤坏死因子和抗炎药物的组合及治疗恶性和非恶性疾病的方法
公开(公告)号：US4980160A
公开(公告)日：1990-12-25
申请号：US373646
申请日：1989-06-29
申请人： Alfred L. Goldberg , Walter C. Fiers , Isis C. Kettelhut
发明人： Alfred L. Goldberg , Walter C. Fiers , Isis C. Kettelhut
IPC分类号： A61K38/19
CPC分类号： A61K38/191 , Y10S514/886
摘要： This invention relates to combinations and methods for the treatment of malignant and non-malignant diseases. More particularly, this invention relates to combinations of natural or recombinant tumor necrosis factors ("TNF") and non-steroidal anti-inflammatory agents, such as indomethacin and ibuprofen, useful for the growth inhibition or killing of transformed cells. According to this invention, the non-steroidal anti-inflammatory agents are used to reduce or eliminate the toxic side effects of high doses of TNFs employed in the treatment of malignant and non-malignant neoplastic diseases. Advantageously, the combinations and methods of this invention allow the administration of higher doses of TNF than those tolerated in conventional treatment regimens based upon TNF alone.
摘要翻译：本发明涉及治疗恶性和非恶性疾病的组合和方法。更具体地说，本发明涉及可用于生长抑制或杀死转化细胞的天然或重组肿瘤坏死因子（“TNF”）和非甾体抗炎药如吲哚美辛和布洛芬的组合。根据本发明，非甾体抗炎剂用于减少或消除用于治疗恶性和非恶性肿瘤疾病的高剂量TNF的毒副作用。有利地，本发明的组合和方法允许施用比基于单独TNF的常规治疗方案中耐受的那些更高剂量的TNF。

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