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1. 发明授权

US5302349A Transient-state luminescence assay apparatus 失效
标题翻译：瞬态发光测定装置
公开(公告)号：US5302349A
公开(公告)日：1994-04-12
申请号：US490770
申请日：1990-03-06
申请人： Walter B. Dandliker , June K. Dandliker , Jacques C. Levin
发明人： Walter B. Dandliker , June K. Dandliker , Jacques C. Levin
IPC分类号： G01N21/64
CPC分类号： G01N21/6445 , G01N21/6408 , G01N21/6428
摘要： Light, pulsed or continuous at a particular wavelength (e.g. 780 nm), fluoresces a specimen. The specimen may be combinations of an antigen (e.g. rubella) labelled with a fluorescent dye, unlabeled antigen or hapten and an antibody reactive with the antigen or hapten. The light polarized in a first direction (e.g. z-axis) parallel to the electric field of the incident light and in a second direction (e.g. x-axis) perpendicular to the first direction is measured. A second specimen is then provided with the antigen and the antibody but without the dye. The same light as discussed above excites the second specimen and polarizes the light. The light polarized in the first (z-axis) and second (x-axis) directions in the second specimen is measured. These measurements are processed in a microprocessor with the measurements in the z and x directions in the first specimen to identify the antigen or, when the antigen is known, to identify the concentration of the antigen in the first specimen. When the light is pulsed, the measurements are made in a time window beginning after the initiation, and terminating before the end, of the fluorescence of the combination of the dye, the antibody and the antigen. Determinations as discussed above but without unlabeled antigen or hapten may be made of the antibody instead of the antigen. When the light is continuous, it is modulated. Measurements are made of the phase shifts in the polarized light in the z and x directions as a result of the light modulations and the decay of the fluorescence.
摘要翻译：在特定波长（例如780nm）下的光，脉冲或连续的荧光发射样品。标本可以是用荧光染料标记的抗原（例如风疹），未标记的抗原或半抗原和与抗原或半抗原反应的抗体的组合。测量在与入射光的电场平行的第一方向（例如z轴）并且垂直于第一方向的第二方向（例如，x轴）偏振的光。然后向抗体和抗体提供第二个标本，但没有染料。与上述相同的光激发第二个样品并使光线偏振。测量在第二样本中在第一（z轴）和第二（x轴）方向偏振的光。这些测量在微处理器中处理，其中在第一样本中的z和x方向上的测量以识别抗原，或者当抗原已知时，以鉴定第一样品中抗原的浓度。当光被脉冲时，测量在起始之后开始的时间窗口中，并且在结束之前终止染料，抗体和抗原的组合的荧光。如上所述但没有未标记的抗原或半抗原的测定可以由抗体代替抗原制成。当光线连续时，它被调制。测量由于光调制和荧光衰减而在z和x方向的偏振光中的相移。

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