专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明专利

CA925838A APPARATUS AND METHOD FOR GRADIENT ZONAL CENTRIFUGATION 未知
公开(公告)号：CA925838A
公开(公告)日：1973-05-08
申请号：CA98042
申请日：1970-11-13
申请人： SHEELER P , WELLS J
发明人： SHEELER P , WELLS J
IPC分类号： B04B1/00 , B04B5/00 , B04B5/04
摘要： 1337398 Centrifugal separators P SHEELER and J R WELLS 18 Dec 1970 [19 Dec 1969 14 Sept 1970] 60335/70 Heading B2P The rotor 22, Figs. 1 and 2, of a gradient zonal centrifuge for separating biological cell components has an internal annular centrifugation chamber bounded by coaxial spaced apart outer and inner vertical walls 42, 43 and divided into compartments by radial septa 51, the bottom or the top of the chamber being formed with sloping walls 44, 46, Fig. 1, or 146, Fig. 8, converging either to an annular V-shaped groove, Fig. 1, or to a plurality of funnel-shaped recesses 142, ports 68 being located at or adjacent the apex 48 or apices 147 of the groove or recesses for supplying and removing liquid density gradient and biological cell components to the chamber. As shown in Figs. 1 and 2, the rotor 22 is mounted on a hub 21 at the upper end of a motor-driven shaft and is closed by a cover plate 35. The septa 51 are integral with a tubular core 52 which is removably mounted on a core portion 23 of the rotor. The bottom of each septum is bounded by converging edges which match the sloping walls 44, 46 of the annular V-shaped groove and terminate in a narrow horizontal edge 63 parallel to, and spaced apart from, the annular apex surface 48 of the groove. This allows liquid communication between adjacent compartments of the chamber. The ports 68 are formed in the lower edges 63 of the septa and communicate with sloping channels 67 in the septa and vertical channels 66 in the core 52. The channels 66 communicate with sloping channels 76 and a central channel 74 in a circular distributor block 71 removably mounted on top of the axial core portion 23 of the rotor and located within an aperture 38 in the cover plate 35. In operation, the liquid density gradient is pumped via tubing through the channels 74, 76, 66, 67 and ports 68 into the chamber compartments. The rotor is then accelerated so that the density gradient undergoes reorientation in which the preliminarily vertically arranged, sector-shaped, isodense layers in each compartment gradually become concentrically arranged about the axis of rotation. A sample of biological cell components may be introduced by initially placing it in the bottom of the V-shaped groove after which it becomes displaced upwardly upon introduction of the density gradient, with both the gradient and the sample underging reorientation during acceleration. Alternatively, after preliminary acceleration of the rotor, the sample may be spread onto the reoriented density gradient through an opening in the cover plate 35. Thereafter, the rotor is accelerated to a higher speed for effecting separation of the cell components into a series of concentric zones about the axis of rotation. The rotor is then brought to rest so that after reorientation the separated cell components are arranged as a series of layers in the now vertical density gradient. Finally, the gradient and the separated particles are pumped from the rotor through the ports 68 and the channels 67, 66, 76, 74 beginning with the dense end of the gradient at the bottom of the V-shaped groove and the cells are collected as a series of fraction. In a modification. Figs. 5-7 (not shown), the top of the chamber opens into an annular V-shaped groove formed in the rotor cover plate and is provided with a flat bottom. In a further modification, Fig. 8, funnelshaped recesses 142 are formed in the bottom of the annular chamber 141 of the rotor 140. The lower portions of the septa (not shown) descend into respective recesses, the parts in the lower edges of the septa being adjacent the apices 147 of the recesses.

2. 发明授权

US3811452A Convertible ash tray-bank 失效
标题翻译：可兑换ASH托盘
公开(公告)号：US3811452A
公开(公告)日：1974-05-21
申请号：US18958171
申请日：1971-10-15
申请人： WELLS J
发明人： WELLS J
IPC分类号： A24F19/10
CPC分类号： A24F19/10
摘要： An ash tray having a base and a cover, the cover adapted to be fastened to the base to form a closed container adapted for saving coins. The cover can be mounted to the base by several different methods. In each method, the cover has a raised position which allows the base to be used as an ash tray. Smoke from an article in the ash tray will rise to the underside of the cover and cause changes in the appearance of the cover. The changes in the appearance of the cover indicate to the owner of the ash tray the quantity of smoking that he engages in.
摘要翻译：一种具有底座和盖子的灰盘，所述盖适于紧固到所述基座以形成适于保存硬币的密闭容器。盖可以通过几种不同的方法安装到基座上。在每种方法中，盖具有允许基部用作灰盘的升高位置。灰托盘中的物品的烟雾将上升到盖的下侧，并导致盖的外观改变。盖子外观的变化表明烟灰缸的所有者他所从事的吸烟量。

3. 发明授权

US3847210A Damper sequencer 失效
标题翻译：阻尼器序列器
公开(公告)号：US3847210A
公开(公告)日：1974-11-12
申请号：US28898472
申请日：1972-09-14
申请人： WELLS J
发明人： WELLS J
IPC分类号： F24F3/048 , F24F13/14 , F28F27/02
CPC分类号： F24F3/048 , F24F13/1413 , F24F13/1426 , F24F2013/1446 , Y10S165/105 , Y10T137/87161 , Y10T137/87467 , Y10T137/87515 , Y10T137/87692
摘要： A fluid-flow apparatus having a damper frame provided with walls forming separate side-by-side flow paths for hot, cold and bypass air. Damper elements are pivotally mounted on shafts and arranged in the flow paths for blocking and unblocking same. Each shaft has a pinion gear mounted thereon for pivotal movement with the shaft and associated damper element. Rack gear segments are provided on a longitudinally slidably mounted carrier bar for engaging the pinion gears in a predetermined sequence which places in a blocked position one of the hot and cold air flow paths whenever the other flow path of that pair is simultaneously in an unblocked position. The bypass air flow path is unblocked when either the hot or cold air flow path is only partially unblocked. Spring or cam elements cooperate with flats on the damper element shaft for releasably retaining them in preselected positions.
摘要翻译：具有阻尼器框架的流体流动装置设置有形成用于热，冷和旁路空气的分开的并排流动路径的壁。阻尼元件枢转地安装在轴上并且布置在流动路径中以阻止和解除阻挡。每个轴具有安装在其上的小齿轮，用于与轴和相关联的阻尼元件枢转运动。机架齿轮段设置在纵向可滑动地安装的行星架上，用于以预定顺序接合小齿轮，预定的顺序，每当该对的另一个流动路径同时处于未阻挡位置。当热或冷空气流动路径仅部分解除阻塞时，旁路空气流动路径被解除阻塞。弹簧或凸轮元件与阻尼器元件轴上的平面配合，以将其可释放地保持在预选位置。

4. 发明授权

US3708111A Apparatus and method for gradient zonal centrifugation 失效
标题翻译：装置和方法用于梯度地形分离
公开(公告)号：US3708111A
公开(公告)日：1973-01-02
申请号：US3708111D
申请日：1970-09-14
申请人： SHEELER P , WELLS J
发明人： SHEELER P , WELLS J
IPC分类号： B04B1/00 , B04B5/00 , B04B5/04
CPC分类号： B04B1/00 , B04B5/00 , B04B5/0407
摘要： A reorienting gradient zonal rotor for separation of cell components and the like, said rotor comprising a cylindrical chamber divided into a plurality of sector-shaped compartments by vertical septa radiating from a central core. Either the annular floor or ceiling of said chamber is formed with sloping converging walls intermediate the spaced outer and inner walls of said chamber to converge either to an annular V-shaped groove or to a plurality of funnel-shaped recesses terminating, respectively, in an annular apex or a plurality of apices at which a plurality of spaced apart gradient removal ports are located, and toward each of which said converging sloping walls cause the gradient zones to be constricted and concentrated for improved definition and separation for subsequent fraction collection and analysis.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式