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    • 7. 发明申请
    • DERIVATIVES OF URIDINE PHOSPHATE AND THEIR USES IN PROTEIN BINDING ASSAYS
    • 磷酸氢钙的衍生物及其在蛋白质结合测定中的应用
    • WO2011051733A3
    • 2011-09-01
    • PCT/GB2010051830
    • 2010-11-01
    • UNIV EAST ANGLIAWAGNER GERDPESNOT THOMAS
    • WAGNER GERDPESNOT THOMAS
    • C07H19/10
    • C07H19/10
    • The present invention relates to compounds and their use in competitive protein binding assays, for example for use with glycosyl transferase and/or glycoprocessing proteins. The present application also provides kits and apparatuses for use in the assays. In particular, the present invention provides a compound of the formula (I): wherein n is 1, 2 or 3; R1 is selected from -OH, -OPO3H, -OR4, -NHR4, R6; R2 and R3 are each independently selected from -H, -OH, optionally substituted -O- alkyl and -O-alkanoyl; R4 is selected from an optionally substituted mono or polysaccharide, -alkyl, -alkenyl, - alkynyl, and L-Z, where L is a linking agent and Z is a binding agent; R6 is an optionally substituted hydrocarbon group; A is either (i) a substituted heteroaryl group, the substituent on the heteroaryl group having a double bond conjugated to the heteroaryl group, or (ii) a substituted aryl group, the substituent on the aryl group having a double bond conjugated to the aryl group.
    • 本发明涉及化合物及其在竞争性蛋白结合测定中的用途,例如用于糖基转移酶和/或糖苷加工蛋白。 本申请还提供了用于测定中的试剂盒和装置。 特别地,本发明提供式(I)化合物:其中n为1,2或3; R 1选自-OH,-OPO 3 H,-OR 4,-NHR 4,R 6; R2和R3各自独立地选自-H,-OH,任选取代的-O-烷基和-O-烷酰基; R4选自任选取代的单或多糖, - 烷基, - 烯基, - 炔基和L-Z,其中L是连接剂,Z是结合剂; R6是任选取代的烃基; A是(i)取代的杂芳基,具有与杂芳基结合的双键的杂芳基上的取代基,或(ii)取代的芳基,具有与芳基共轭的双键的芳基上的取代基 组。