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1. 发明申请

US20130097728A1 AXMI115 VARIANT INSECTICIDAL GENE AND METHODS FOR ITS USE 审中-公开
标题翻译： AXMI115变种杀虫剂基因及其使用方法
公开(公告)号：US20130097728A1
公开(公告)日：2013-04-18
申请号：US13439269
申请日：2012-04-04
申请人： Volker Heinrichs , Duane Lehtinen
发明人： Volker Heinrichs , Duane Lehtinen
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , A01N37/44 , A01N63/02 , C07K14/32 , C07K14/325 , C07K2319/00 , C07K2319/24 , C12N15/8279 , C12N15/8285 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. The toxin coding sequences can be used in DNA constructs or expression cassettes for expression in plants and bacteria. Compositions include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, polynucleotide sequences and the toxin proteins encoded thereby are provided. Also provided are antibodies specifically binding to those amino acid sequences. In particular, the invention encompasses nucleotide sequences encoding fusion proteins, as well as biologically active variants and fragments thereof, wherein the fusion protein contains the C-terminal portion of SEQ ID NO:43. The fusion protein may also contain the N-terminal portion of SEQ ID NO:45. The invention also includes the nucleotide sequence of SEQ ID NO:47 and 1-14, or a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:48 and 15-31, including biologically active variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。毒素编码序列可用于DNA构建体或表达盒中用于在植物和细菌中表达。组合物包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了多核苷酸序列和由此编码的毒素蛋白质。还提供了与这些氨基酸序列特异性结合的抗体。特别地，本发明包括编码融合蛋白的核苷酸序列，以及其生物活性变体及其片段，其中融合蛋白含有SEQ ID NO：43的C-末端部分。融合蛋白还可含有SEQ ID NO：45的N-末端部分。本发明还包括SEQ ID NO：47和1-14的核苷酸序列，或编码SEQ ID NO：48和15-31所示氨基酸序列的核苷酸序列，包括生物活性变体和片段。

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