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1. 发明申请

WO2006119265A2 XENOGRAFT TISSUE CONTROL FOR HISTOLOGY 审中-公开
标题翻译： XENOGRAFT组织控制组织学
公开(公告)号：WO2006119265A2
公开(公告)日：2006-11-09
申请号：PCT/US2006/016766
申请日：2006-05-01
申请人： VENTANA MEDICAL SYSTEMS, INC. , HARDY, Margaret , GROGAN, Thomas, M. , NAGLE, Ray, B.
发明人： HARDY, Margaret , GROGAN, Thomas, M. , NAGLE, Ray, B.
IPC分类号： G01N33/50 , G01N33/577 , G01N33/567
CPC分类号： G01N33/5005 , G01N1/30 , G01N1/312 , G01N2001/2893 , G01N2015/1018
摘要： An embodiment of the invention is a method of using a xenograft as a control tissue for histology, comprising staining both a patient and a xenograft-derived control sample under substantially similar staining conditions, and assessing the staining outcomes of the two to determine whether the stain was effective for the patient sample. A xenograft has never been used before in histology as a control, as far as the inventors know. The result of using a xenograft as a control is surprisingly advantageous. First, the cell lines grow and differentiate similarly to a human, taking on the general morphology of a real tissue sample. Second, because the same transformed cell line can be grown limitless times in SCID mice, the xenograft control is highly reproducible, leading to a consistent artificial control that is highly manufacturable and subject to genetic manipulation so that antigens or genetic elements may be embedded in the tissue. Another embodiment of the invention is directed generally to a method of making a tissue control substrate, comprising growing a xenograft from a mammalian transformed cell line in a host animal, removing the xenograft from the host animal, processing the xenograft thereby embedding the xenograft tissue in an embedding medium, and finally affixing the embedded xenograft sample onto a substrate. The substrate is generally a microscope slide. The xenograft control slide can then be stained side-by-side with a specimen sample in an automated slide stainer, and act as a control against which the staining quality can be compared. The xenograft control can also be used as a manual staining control. Determining whether the staining was effective for the patient specimen comprises judging the staining intensity of the xenograft control sample to determine if the expected degree and type of staining were realized in the control. If the expected type (nuclear, membranous, or cytoplasmic) and degree (0-4 scale) of staining are realized during the run, then the xenograft control indicates the staining process and reagents are working properly, and so the result in the patient specimen can be trusted. A further embodiment of the invention is a xenograft-derived control slide for histochemical use, comprising at least one xenograft control sample prepared for histological use, and a sample slide upon which the at least one xenograft control sample is affixed.
摘要翻译：本发明的一个实施方案是使用异种移植物作为组织学的对照组织的方法，包括在基本相似的染色条件下染色患者和异种移植物衍生的对照样品，并评估两者的染色结果以确定染色对患者样本有效。就本发明人所知，异种移植物在组织学之前从未被用作对照。使用异种移植物作为对照的结果是惊人的有利的。首先，细胞系与人类相似地生长和分化，承担真实组织样品的一般形态。第二，由于相同的转化细胞系可以在SCID小鼠中无限期地生长，异种移植物控制是高度可重复的，导致一致的人造控制，其是高度可制造的并经受遗传操作，使得抗原或遗传元件可嵌入组织。本发明的另一个实施方案一般涉及一种制备组织对照底物的方法，包括从宿主动物的哺乳动物转化细胞系中生长异种移植物，从宿主动物中除去异种移植物，加工异种移植物，从而将异种移植组织嵌入嵌入介质，最后将嵌入的异种移植样品固定在基底上。基底通常是显微镜载玻片。然后将异种移植物对照载玻片与自动化载玻片染色剂中的标本样品并排染色，并且作为可以比较染色质量的对照。异种移植物控制也可以用作手动染色控制。确定染色对患者标本是否有效包括判断异种移植物对照样品的染色强度，以确定在对照中是否实现预期的染色程度和染色类型。如果在运行期间实现预期的类型（核，膜或细胞质）和染色程度（0-4标度），则异种移植物对照表明染色过程和试剂正常工作，因此导致患者标本可以信任本发明的另一个实施方案是用于组织化学用途的异种移植物衍生的对照载玻片，其包含至少一种用于组织学用途制备的异种移植物对照样品和载有至少一种异种移植物对照样品的样品载玻片。

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