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    • 3. 发明授权
    • Modulation of cytokinin activity in plants
    • 植物中细胞分裂素活性的调节
    • US07531723B2
    • 2009-05-12
    • US10817483
    • 2004-04-02
    • Jeffrey E. HabbenChris ZinselmeierDwight T. TomesTimothy G. Helentjaris
    • Jeffrey E. HabbenChris ZinselmeierDwight T. TomesTimothy G. Helentjaris
    • C12N15/82A01H5/00A01H5/10
    • C12N15/8234C12N9/0032C12N9/1085C12N15/8243C12N15/8261C12N15/8271C12N15/8295Y02A40/146
    • This invention relates generally to the field of plant molecular biology. More specifically, this invention relates to methods and reagents for the temporally- and/or spatially-regulated expression of genes that affect metabolically effective levels of cytokinins in plants, particularly in plant seeds and related female reproductive tissue. This invention further relates to transgenic plants having enhanced levels of cytokinin expression wherein the transgenic plant exhibits useful characteristics, such as improved seed size, decreased tip kernel abortion, increased seed set during unfavorable environmental conditions, or stability of yield. The present invention also provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions comprise novel nucleotide sequences for seed-preferred promoters known as eep1 and eep2. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell.
    • 本发明一般涉及植物分子生物学领域。 更具体地说,本发明涉及影响植物中特别是植物种子和相关雌性生殖组织中细胞分裂素的代谢有效水平的基因的时间和/或空间调节表达的方法和试剂。 本发明进一步涉及具有增强的细胞分裂素表达水平的转基因植物,其中转基因植物表现出有用的特征,例如改善的种子大小,降低的末梢核仁流产,在不利的环境条件下增加的种子集合或产量的稳定性。 本发明还提供用于调节植物中异源核苷酸序列表达的组合物和方法。 组合物包含已知为eep1和eep2的种子优选启动子的新型核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括转化植物细胞以包含与本发明的启动子之一可操作地连接的异源核苷酸序列,并从转化的植物细胞再生稳定转化的植物。