专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2012003478A2 DETECTING TARGETS USING MASS TAGS AND MASS SPECTROMETRY 审中-公开
标题翻译：用质量标签和质谱法检测目标
公开(公告)号：WO2012003478A2
公开(公告)日：2012-01-05
申请号：PCT/US2011/042853
申请日：2011-07-01
申请人： VENTANA MEDICAL SYSTEMS, INC. , HONG, Rui , WANG, Hong , LEFEVER, Mark , FROEHLICH, Jan , BIENIARZ, Christopher , KELLY, Brian , MILLER, Phillip
发明人： HONG, Rui , WANG, Hong , LEFEVER, Mark , FROEHLICH, Jan , BIENIARZ, Christopher , KELLY, Brian , MILLER, Phillip
IPC分类号： A61K49/00
CPC分类号： G01N33/6848 , G01N27/62
摘要： Particular disclosed embodiments disclosed herein concern using a one or more various mass tags, which can be specifically deposited at targets through direct or indirect enzymatic-catalyzed transformation, to provide a method for identifying targets in tissue samples. The mass tags may be labeled with stable isotopes to produce mass tags having the same chemical structure but different masses. Mass codes produced by ionizing the mass tags are detected and/or quantified using mass spectrometry. The method can be used for multiplexed detection of multiple targets in a particular sample. In some embodiments, a map divided into sections representing sections of the tissue sample may be prepared, with the map sections including data corresponding to quantification data wherein the size of a mass peak is determined and correlated with the amount of a target for the corresponding tissue sample section.
摘要翻译：本文公开的具体公开的实施方案涉及使用一种或多种不同的质量标签，其可以通过直接或间接的酶促催化转化特异性地沉积在靶标上，以提供用于鉴定组织样品中的靶标的方法。质量标签可以用稳定的同位素标记以产生具有相同化学结构但质量不同的质量标签。通过电离质量标签产生的质量代码使用质谱法检测和/或量化。该方法可用于特定样本中多个目标的多路复用检测。在一些实施例中，可以制备分成代表组织样本的部分的区段的地图，其中地图区段包括对应于定量数据的数据，其中确定质量峰的大小并将其与相应组织的目标的量相关联样本部分。

2. 发明申请

WO2014139980A1 PROXIMITY ASSAY FOR IN SITU DETECTION OF TARGETS 审中-公开
标题翻译：用于现场检测目标的接近度测定
公开(公告)号：WO2014139980A1
公开(公告)日：2014-09-18
申请号：PCT/EP2014/054634
申请日：2014-03-11
申请人： VENTANA MEDICAL SYSTEMS, INC. , F. HOFFMANN-LA ROCHE AG
发明人： FARRELL, Michael , HONG, Rui , JIANG, Zeyu (David)
IPC分类号： C12Q1/25 , G01N33/535 , G01N33/58
CPC分类号： C12Q1/25 , G01N33/535 , G01N33/581 , G01N2333/9015 , G01N2440/32
摘要： A proximity detection method is described that utilizes enzymatic biotinylation to detect targets in a sample, particularly formalin fixed paraffin embedded samples using automated staining platforms. One disclosed embodiment comprises contacting the sample with a first conjugate comprising a biotin ligase and a first specific binding moiety that binds proximally to the first target; contacting the sample with a second conjugate comprising a biotin ligase substrate and a second specific binding moiety that binds proximally to the second target; subjecting the sample to conditions that allow biotinylation of the biotin ligase substrate by the biotin ligase when the first target and the second target have a proximal arrangement; and detecting biotinylation of the biotin ligase substrate. The conditions that allow biotinylation of the substrate include addition of biotin and ATP. The method also may comprise contacting the sample with a streptavidin-enzyme conjugate. Signal amplification also can be used.
摘要翻译：描述了一种接近检测方法，其利用酶生物素化来检测样品中的靶标，特别是使用自动染色平台的福尔马林固定石蜡包埋样品。一个公开的实施方案包括使样品与包含生物素连接酶的第一缀合物和与第一靶近端结合的第一特异性结合部分接触; 使样品与包含生物素连接酶底物和第二特异性结合部分的第二缀合物接触，所述第二特异性结合部分与第二靶标近端结合; 当所述第一靶和所述第二靶具有近端排列时，使所述样品经受生物素连接酶使生物素连接酶底物生物素化的条件; 并检测生物素连接酶底物的生物素化。允许底物生物素化的条件包括生物素和ATP的添加。该方法还可以包括使样品与链霉亲和素 - 酶缀合物接触。信号放大也可以使用。

3. 发明申请

WO2019120635A1 PEPTIDE NUCLEIC ACID CONJUGATES 审中-公开
公开(公告)号：WO2019120635A1
公开(公告)日：2019-06-27
申请号：PCT/EP2018/065697
申请日：2018-06-13
申请人： VENTANA MEDICAL SYSTEMS, INC. , F. HOFFMANN-LA ROCHE AG
发明人： BARHOUMI, Aoune , HONG, Rui
IPC分类号： A61K49/00 , C12Q1/6816 , C12Q1/6823 , G01N33/532 , G01N33/58 , A61K47/68
摘要： The present disclosure is directed to conjugates of a specific binding entity and an oligomer, i.e. [Specific Binding Entity]-[Oligomer] n , wherein n is an integer ranging from 1 to 12, and where the Oligomer includes, in some embodiments, a PNA sequence having at least one substituent at a gamma carbon position. In some embodiments, the substituent at the gamma carbon position, e.g. an amino acid, a peptide, a miniPEG, or a polymer, includes at least one reporter moiety.

4. 发明申请

WO2018118759A1 PEPTIDE NUCLEIC ACID CONJUGATES 审中-公开
公开(公告)号：WO2018118759A1
公开(公告)日：2018-06-28
申请号：PCT/US2017/066976
申请日：2017-12-18
申请人： VENTANA MEDICAL SYSTEMS, INC.
发明人： HONG, Rui , BARHOUMI, Aoune
IPC分类号： A61K47/68 , A61K49/00 , G01N33/58 , C12Q1/6823
摘要： The disclosure is directed to conjugates, e.g. PNA conjugates, as well as methods of employing the conjugates for detecting one or more targets in a biological sample, e.g. a tissue sample.

5. 发明申请

WO2020058339A1 COUMARIN-BASED CROSSLINKING REAGENTS 审中-公开
公开(公告)号：WO2020058339A1
公开(公告)日：2020-03-26
申请号：PCT/EP2019/075013
申请日：2019-09-18
申请人： VENTANA MEDICAL SYSTEMS, INC. , F. HOFFMANN-LA ROCHE AG
发明人： HONG, Rui , LEFEVER, Mark , MAY, Eric
IPC分类号： C07D311/16 , C07D405/12 , C07D405/14 , C07D407/12 , C07D413/12 , C07D413/14 , C07D417/12 , C07D417/14 , C09B57/02 , C12Q1/68 , G01N33/53
摘要： Disclosed herein are novel coumarin-based reagents, e.g. linkers, and conjugates including one or more of the disclosed coumarin-based reagents. In some embodiments, the presence of a coumarin moiety within the coumarin-based reagents enables detection of labels which are typically difficult to detect, e.g. certain haptens.

6. 发明申请

WO2016083364A1 PROXIMITY ASSAYS USING CHEMICAL LIGATION AND HAPTEN TRANSFER 审中-公开
标题翻译：使用化学物质和转移的亲和力测定
公开(公告)号：WO2016083364A1
公开(公告)日：2016-06-02
申请号：PCT/EP2015/077484
申请日：2015-11-24
申请人： VENTANA MEDICAL SYSTEMS, INC. , F. HOFFMANN-LA ROCHE AG
发明人： BIENIARZ, Christopher , HONG, Rui
IPC分类号： G01N33/543 , G01N33/574 , G01N33/68
CPC分类号： G01N33/6845 , G01N33/542 , G01N33/54353 , G01N2440/00
摘要： Methods for in situ detecting proximity of two targets of interest featuring an antibody conjugated with a cleavable bridge component having a detectable moiety and an antibody conjugated with a non-cleavable bridge component. The bridge components each have a chemical ligation group adapted to form a covalent bond under particular conditions and when the targets are in close proximity. Following covalent bond formation, the cleavable bridge component can be cleaved from the antibody, effectively transferring the detectable moiety to the non-cleavable bridge component. Detection of the detectable moiety is indicative of the targets being in close proximity. The methods are compatible with both chromogenic and fluorogenic detection systems. The methods may be used to perform assays wherein one or more than one proximity event is detected on the same slide.
摘要翻译：用于原位检测感兴趣的两个靶标的亲和力的方法，其特征在于具有可检测部分的可切割桥组件的抗体和与不可裂解的桥组件缀合的抗体。桥组件各自具有适于在特定条件下形成共价键的化学连接基团，并且当靶物接近时。在共价键形成之后，可切割的桥组件可以从抗体切割，有效地将可检测部分转移到不可裂解的桥组件。可检测部分的检测表明目标处于非常接近的位置。该方法与显色和荧光检测系统兼容。该方法可用于进行其中在同一载玻片上检测到一个或多于一个邻近事件的测定。

7. 发明申请

WO2014079802A2 LASER ABLATION INDUCTIVELY-COUPLED PLASMA MASS SPECTRAL TISSUE DIAGNOSTICS 审中-公开
标题翻译：激光烧蚀电感耦合等离子体质谱组织诊断
公开(公告)号：WO2014079802A2
公开(公告)日：2014-05-30
申请号：PCT/EP2013/074049
申请日：2013-11-18
申请人： VENTANA MEDICAL SYSTEMS, INC. , F. HOFFMANN-LA ROCHE AG
发明人： BIENIARZ, Christopher , HONG, Rui , MILLER, Phillip C
IPC分类号： H01J49/00
CPC分类号： H01J49/0004 , H01J49/0409 , H01J49/0463 , H01J49/105
摘要： Methods, kits, and systems for multiplexed tissue diagnostics of a histopathological sample using mass tags are disclosed. A method of analyzing a sample using mass spectral imaging correlated to a digital image of the sample visualized using optical staining enables multiplexed tissue analysis at various resolutions with medically relevant context. Compositions for multiplexed tissue laser ablation inductively coupled plasma mass spectrometry include mass tags, for example elemental mass tags, conjugated to a specific binding moiety. The mass tag includes elements that are detectably distinct from elements endogenous to tissue.
摘要翻译：公开了使用质量标签对组织病理学样品进行多路复用组织诊断的方法，试剂盒和系统。使用与使用光学染色可视化的样品的数字图像相关联的质谱成像来分析样品的方法使得能够在具有医学相关背景的各种分辨率下进行多路复用组织分析。用于多路复用组织激光消融的组合物电感耦合等离子体质谱包括与特异性结合部分缀合的质量标签，例如元素质量标签。质量标签包含与组织内源性元素明显不同的元素。

8. 发明公开

EP2971064A1 PROXIMITY ASSAY FOR IN SITU DETECTION OF TARGETS 有权
标题翻译： NÄHERUNGSTESTFÜRIN-SITU-DETEKTION VON ZIELEN
公开(公告)号：EP2971064A1
公开(公告)日：2016-01-20
申请号：EP14708893.4
申请日：2014-03-11
申请人： Ventana Medical Systems, Inc.
发明人： FARRELL, Michael , HONG, Rui , JIANG, Zeyu (David)
IPC分类号： C12Q1/25 , G01N33/535 , G01N33/58
摘要： A proximity detection method is described that utilizes enzymatic biotinylation to detect targets in a sample, particularly formalin fixed paraffin embedded samples using automated staining platforms. One disclosed embodiment comprises contacting the sample with a first conjugate comprising a biotin ligase and a first specific binding moiety that binds proximally to the first target; contacting the sample with a second conjugate comprising a biotin ligase substrate and a second specific binding moiety that binds proximally to the second target; subjecting the sample to conditions that allow biotinylation of the biotin ligase substrate by the biotin ligase when the first target and the second target have a proximal arrangement; and detecting biotinylation of the biotin ligase substrate. The conditions that allow biotinylation of the substrate include addition of biotin and ATP. The method also may comprise contacting the sample with a streptavidin-enzyme conjugate. Signal amplification also can be used.
摘要翻译：描述了一种接近检测方法，其利用酶生物素化来检测样品中的靶标，特别是使用自动染色平台的福尔马林固定石蜡包埋样品。一个公开的实施方案包括使样品与包含生物素连接酶的第一缀合物和与第一靶近端结合的第一特异性结合部分接触; 使所述样品与包含生物素连接酶底物和第二特异性结合部分的第二缀合物接触，所述第二特异性结合部分向近端结合至所述第二靶标; 当所述第一靶和所述第二靶具有近端排列时，使所述样品经过生物素连接酶使生物素连接酶底物生物素化的条件; 并检测生物素连接酶底物的生物素化。允许底物生物素化的条件包括生物素和ATP的添加。该方法还可以包括使样品与链霉亲和素 - 酶缀合物接触。信号放大也可以使用。

9. 发明授权

EP2588144B1 DETECTING TARGETS USING MASS TAGS AND MASS SPECTROMETRY 有权
标题翻译：用质量标签和质谱法检测目标
公开(公告)号：EP2588144B1
公开(公告)日：2018-05-09
申请号：EP11731604.2
申请日：2011-07-01
申请人： Ventana Medical Systems, Inc.
发明人： HONG, Rui , WANG, Hong , LEFEVER, Mark , FROEHLICH, Jan , BIENIARZ, Christopher , KELLY, Brian Daniel , MILLER, Phillip
IPC分类号： A61K49/00 , G01N33/543 , G01N33/68 , G01N33/58 , C12Q1/28 , G01N33/532
CPC分类号： G01N33/6848 , G01N27/62
摘要： Particular disclosed embodiments disclosed herein concern using a one or more various mass tags, which can be specifically deposited at targets through direct or indirect enzymatic-catalyzed transformation, to provide a method for identifying targets in tissue samples. The mass tags may be labeled with stable isotopes to produce mass tags having the same chemical structure but different masses. Mass codes produced by ionizing the mass tags are detected and/or quantified using mass spectrometry. The method can be used for multiplexed detection of multiple targets in a particular sample. In some embodiments, a map divided into sections representing sections of the tissue sample may be prepared, with the map sections including data corresponding to quantification data wherein the size of a mass peak is determined and correlated with the amount of a target for the corresponding tissue sample section.

10. 发明公开

EP3727470A1 PEPTIDE NUCLEIC ACID CONJUGATES 审中-公开
公开(公告)号：EP3727470A1
公开(公告)日：2020-10-28
申请号：EP18730359.9
申请日：2018-06-13
申请人： Ventana Medical Systems, Inc.
发明人： BARHOUMI, Aoune , HONG, Rui
IPC分类号： A61K49/00 , C12Q1/6816 , C12Q1/6823 , G01N33/532 , G01N33/58 , A61K47/68

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式