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1. 发明申请

WO2016166073A1 THERMOCHEMICAL-BASED ANTIBODY INACTIVATION METHODS AND SYSTEMS 审中-公开
标题翻译：基于热化学的抗体灭活方法和系统
公开(公告)号：WO2016166073A1
公开(公告)日：2016-10-20
申请号：PCT/EP2016/057955
申请日：2016-04-12
申请人： VENTANA MEDICAL SYSTEMS, INC. , F. HOFFMANN-LA ROCHE AG
发明人： HUBBARD, Antony , JONES, Tobin , TANG, Lei , ZHANG, Wenjun
IPC分类号： G01N33/543 , G01N1/30 , G01N1/31
CPC分类号： G01N33/54393 , G01N1/30 , G01N1/312 , G01N2333/435 , G01N2333/70596
摘要： Methods, systems, compositions, and thermochemical processes for inactivating proteinaceous binding entities (PBEs) such as antibodies in between staining cycles for preventing cross-reactivity in subsequent staining cycles. The thermochemical methods, compositions, or systems may feature incubating a sample in a solution comprising a buffer at a temperature sufficient to reduce or eliminate further detectability of the PBE. The methods, compositions, and systems may be beneficial for automated assays in automated staining devices that are adapted to dispense buffers and reagents and to heat samples to various temperatures.
摘要翻译：用于在染色周期之间灭活蛋白质结合实体（PBEs）如抗体的方法，系统，组合物和热化学方法，以防止随后染色周期中的交叉反应性。热化学方法，组合物或系统可以包括在足以降低或消除PBE的进一步检测能力的温度下在包含缓冲液的溶液中孵育样品。所述方法，组合物和系统对于适于分配缓冲液和试剂以及将样品加热到各种温度的自动染色装置中的自动化测定可能是有益的。

2. 发明申请

WO2014139979A1 QUANTUM DOT IN SITU HYBRIDIZATION 审中-公开
标题翻译：原子杂交中的量子点
公开(公告)号：WO2014139979A1
公开(公告)日：2014-09-18
申请号：PCT/EP2014/054633
申请日：2014-03-11
申请人： VENTANA MEDICAL SYSTEMS, INC. , F. HOFFMANN-LA ROCHE AG
发明人： HUBBARD, Antony , TANG, Lei , ZHANG, Wenjun
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6841 , B82Y15/00 , C12Q2537/143 , C12Q2563/131 , C12Q2563/155 , C12Q2527/125
摘要： An improved quantum dot in situ hybridization method for reliably and reproducibly detecting targets is disclosed. Certain disclosed embodiments concern an in situ detection method utilizing a modified paraffin removal process, as well as a pretreatment step prior to the addition of probes. Some embodiments use a protease and either an acidic buffer treatment, a basic buffer treatment, or both. The method also may comprise using a chelating agent in process solutions to remove metal/metal ion contaminants. After pretreatment, at least one probe comprising a hapten conjugated to a specific binding moiety is added to the sample. After hybridizing the probe to the target a second conjugate comprising an anti-hapten antibody and quantum dot is applied to the sample. After washing, the sample is exposed to light to excite the quantum dot(s). Fluorescence signals from the quantum dot(s) are then detected and identified by obtaining a series of monochrome images, such as by using filters to selectively detect each quantum dot signal. These images are then colorized and stacked to produce a final combined image. Disclosed embodiments provide a robust and reliable QD ISH process having reduced slide background to facilitate analysis.
摘要翻译：公开了一种用于可靠和可重复检测目标的改进的量子点原位杂交方法。某些公开的实施方案涉及使用改性的石蜡去除方法的原位检测方法以及在加入探针之前的预处理步骤。一些实施方案使用蛋白酶和酸性缓冲液处理，碱性缓冲液处理或两者。该方法还可以包括在工艺溶液中使用螯合剂以除去金属/金属离子污染物。预处理后，将至少一个包含与特异性结合部分缀合的半抗原的探针加入到样品中。将探针与目标物杂交后，将包含抗半抗原抗体和量子点的第二偶联物施加到样品上。洗涤后，将样品曝光以激发量子点。然后通过获得一系列单色图像来检测和识别来自量子点的荧光信号，例如通过使用滤波器来选择性地检测每个量子点信号。然后将这些图像着色并堆叠以产生最终的组合图像。公开的实施例提供了具有减少的幻灯片背景以便于分析的鲁棒且可靠的QD ISH过程。

3. 发明申请

WO2016020429A1 SINGLE-STRANDED OLIGONUCLEOTIDE PROBES FOR CHROMOSOME OR GENE COPY ENUMERATION 审中-公开
标题翻译：用于染色体或基因拷贝的单链寡核苷酸探针
公开(公告)号：WO2016020429A1
公开(公告)日：2016-02-11
申请号：PCT/EP2015/068041
申请日：2015-08-05
申请人： VENTANA MEDICAL SYSTEMS, INC. , F. HOFFMANN-LA ROCHE AG
发明人： HUBBARD, Antony , TANG, Lei , ZHANG, Wenjun , BACA PARKINSON, Lesli
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q2600/156 , C12Q2600/158
摘要： Single-stranded oligonucleotide probes, systems, kits and methods for chromosome enumeration, gene copy enumeration, or tissue diagnostics. The probes are particularly suited for detecting gene amplification, deletion, or rearrangement in tissue samples in a single, dual, or multiplexed assay. The probes exhibit improved performance compared to industry leading dual-stranded probes; particularly in terms of the rate of hybridization and the ability to achieve specific hybridization without blocking DNA.
摘要翻译：用于染色体计数，基因拷贝计数或组织诊断的单链寡核苷酸探针，系统，试剂盒和方法。探针特别适用于单次，双重或多重测定中检测组织样品中的基因扩增，缺失或重排。与行业领先的双链探针相比，探针表现出改善的性能; 特别是在杂交速率和在不阻断DNA的情况下实现特异性杂交的能力。

4. 发明申请

WO2015124702A1 SINGLE-STRANDED OLIGONUCLEOTIDE PROBES FOR CHROMOSOME OR GENE COPY ENUMERATION 审中-公开
标题翻译：用于染色体或基因拷贝的单链寡核苷酸探针
公开(公告)号：WO2015124702A1
公开(公告)日：2015-08-27
申请号：PCT/EP2015/053555
申请日：2015-02-20
申请人： VENTANA MEDICAL SYSTEMS, INC. , F. HOFFMANN-LA ROCHE AG
发明人： FARRELL, Michael , HUBBARD, Antony , JOHNSON, Donald , KELLY, Brian D. , SHINGLER, Taylor , TANG, Lei , ZHANG, Wenjun
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6841 , C12Q2600/156 , C12Q2600/158 , C12Q2537/143 , C12Q2537/16 , C12Q2537/164
摘要： Single-stranded oligonucleotide probes, systems, kits and methods for chromosome enumeration, gene copy enumeration, or tissue diagnostics. The probes are particularly suited for detecting gene amplification, deletion, or rearrangement in tissue samples in a single, dual, or multiplexed assay. The probes exhibit improved performance compared to industry leading dual-stranded probes; particularly in terms of the rate of hybridization.
摘要翻译：用于染色体计数，基因拷贝计数或组织诊断的单链寡核苷酸探针，系统，试剂盒和方法。探针特别适用于单次，双重或多重测定中检测组织样品中的基因扩增，缺失或重排。与行业领先的双链探针相比，探针表现出改善的性能; 特别是在杂交速率方面。

5. 发明申请

WO2017155996A1 MULTIPLEXED IMMUNOHISTOCHEMISTRY USING RECOMBINANT ANTIBODIES WITH EPITOPE TAGS 审中-公开
标题翻译：使用重组抗体与EPITOPE标签复合免疫组织化学
公开(公告)号：WO2017155996A1
公开(公告)日：2017-09-14
申请号：PCT/US2017/021157
申请日：2017-03-07
申请人： VENTANA MEDICAL SYSTEMS, INC.
发明人： FARRELL, Michael , MAY, Eric , TANG, Lei , ZHANG, Wenjun , HUBBARD, Antony , TSAO, Tsu-Shuen
IPC分类号： C07K16/18 , C07K16/28 , C07K16/32 , G01N33/50
摘要： The present disclosure is directed to epitope-tagged antibodies, as well as methods of employing the epitope-tagged antibodies for detecting one or more targets in a biological sample, e.g. a tissue sample.
摘要翻译：本公开涉及表位标记的抗体，以及使用表位标记的抗体用于检测生物样品中的一个或多个靶标的方法，例如，一个组织样本。

6. 发明授权

EP3177733B1 SINGLE-STRANDED OLIGONUCLEOTIDE PROBES FOR CHROMOSOME OR GENE COPY ENUMERATION 有权
公开(公告)号：EP3177733B1
公开(公告)日：2018-09-26
申请号：EP15747477.6
申请日：2015-08-05
申请人： Ventana Medical Systems, Inc.
发明人： HUBBARD, Antony , TANG, Lei , ZHANG, Wenjun , BACA PARKINSON, Lesli
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q2600/156 , C12Q2600/158
摘要： Single-stranded oligonucleotide probes, systems, kits and methods for chromosome enumeration, gene copy enumeration, or tissue diagnostics. The probes are particularly suited for detecting gene amplification, deletion, or rearrangement in tissue samples in a single, dual, or multiplexed assay. The probes exhibit improved performance compared to industry leading dual-stranded probes; particularly in terms of the rate of hybridization and the ability to achieve specific hybridization without blocking DNA.

7. 发明公开

EP3283881A1 THERMOCHEMICAL-BASED ANTIBODY INACTIVATION METHODS AND SYSTEMS 有权
标题翻译：基于热化学的抗体失活方法和系统
公开(公告)号：EP3283881A1
公开(公告)日：2018-02-21
申请号：EP16715850.0
申请日：2016-04-12
申请人： Ventana Medical Systems, Inc.
发明人： HUBBARD, Antony , JONES, Tobin , TANG, Lei , ZHANG, Wenjun
IPC分类号： G01N33/543 , G01N1/30 , G01N1/31
CPC分类号： G01N33/54393 , G01N1/30 , G01N1/312 , G01N2333/435 , G01N2333/70596
摘要： Methods, systems, compositions, and thermochemical processes for inactivating proteinaceous binding entities (PBEs) such as antibodies in between staining cycles for preventing cross-reactivity in subsequent staining cycles. The thermochemical methods, compositions, or systems may feature incubating a sample in a solution comprising a buffer at a temperature sufficient to reduce or eliminate further detectability of the PBE. The methods, compositions, and systems may be beneficial for automated assays in automated staining devices that are adapted to dispense buffers and reagents and to heat samples to various temperatures.

8. 发明公开

EP3108006A1 SINGLE-STRANDED OLIGONUCLEOTIDE PROBES FOR CHROMOSOME OR GENE COPY ENUMERATION 有权
标题翻译： EINZELSTRÄNGIGEOLIGONUKLEOTIDSONDENFÜRCHROMOSOMEN- ODERGENKOPIEZÄHLUNG
公开(公告)号：EP3108006A1
公开(公告)日：2016-12-28
申请号：EP15705997.3
申请日：2015-02-20
申请人： Ventana Medical Systems, Inc.
发明人： FARRELL, Michael , HUBBARD, Antony , JOHNSON, Donald , KELLY, Brian D. , SHINGLER, Taylor , TANG, Lei , ZHANG, Wenjun
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6841 , C12Q2600/156 , C12Q2600/158 , C12Q2537/143 , C12Q2537/16 , C12Q2537/164
摘要： Single-stranded oligonucleotide probes, systems, kits and methods for chromosome enumeration, gene copy enumeration, or tissue diagnostics. The probes are particularly suited for detecting gene amplification, deletion, or rearrangement in tissue samples in a single, dual, or multiplexed assay. The probes exhibit improved performance compared to industry leading dual-stranded probes; particularly in terms of the rate of hybridization.
摘要翻译：用于染色体计数，基因拷贝计数或组织诊断的单链寡核苷酸探针，系统，试剂盒和方法。探针特别适用于单次，双重或多重测定中检测组织样品中的基因扩增，缺失或重排。与行业领先的双链探针相比，探针表现出改善的性能; 特别是在杂交速率方面。

9. 发明公开

EP3426684A1 MULTIPLEXED IMMUNOHISTOCHEMISTRY USING RECOMBINANT ANTIBODIES WITH EPITOPE TAGS 审中-实审
公开(公告)号：EP3426684A1
公开(公告)日：2019-01-16
申请号：EP17712344.5
申请日：2017-03-07
申请人： Ventana Medical Systems, Inc.
发明人： FARRELL, Michael , MAY, Eric , TANG, Lei , ZHANG, Wenjun , HUBBARD, Antony , TSAO, Tsu-Shuen
IPC分类号： C07K16/18 , C07K16/28 , C07K16/32 , G01N33/50

10. 发明授权

EP3108006B1 SINGLE-STRANDED OLIGONUCLEOTIDE PROBES FOR CHROMOSOME OR GENE COPY ENUMERATION 有权
公开(公告)号：EP3108006B1
公开(公告)日：2018-08-01
申请号：EP15705997.3
申请日：2015-02-20
申请人： Ventana Medical Systems, Inc.
发明人： FARRELL, Michael , HUBBARD, Antony , JOHNSON, Donald , KELLY, Brian D. , SHINGLER, Taylor , TANG, Lei , ZHANG, Wenjun
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6841 , C12Q2600/156 , C12Q2600/158 , C12Q2537/143 , C12Q2537/16 , C12Q2537/164
摘要： Single-stranded oligonucleotide probes, systems, kits and methods for chromosome enumeration, gene copy enumeration, or tissue diagnostics. The probes are particularly suited for detecting gene amplification, deletion, or rearrangement in tissue samples in a single, dual, or multiplexed assay. The probes exhibit improved performance compared to industry leading dual-stranded probes; particularly in terms of the rate of hybridization.

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