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1. 发明申请

WO2010044101A1 PURIFICATION AND ASSAY FOR TOPOISOMERASES AND USE OF THE ASSAY FOR SCREENING MODULATORS OF TOPOISOMERASES 审中-公开
标题翻译：用于淀粉样物质的纯化和测定以及测定用于筛选淀粉样蛋白的调节剂的方法
公开(公告)号：WO2010044101A1
公开(公告)日：2010-04-22
申请号：PCT/IN2009/000561
申请日：2009-10-08
申请人： V. B. MEDICARE PVT. LTD. , GUPTA, Munishwar, Nath , SINGH, Pradeep, K. , RAGHAVA, Smita
发明人： GUPTA, Munishwar, Nath , SINGH, Pradeep, K. , RAGHAVA, Smita
IPC分类号： C12N9/90 , C12Q1/18 , C12Q1/533 , C12Q1/68 , G01N21/64 , G01N33/68
CPC分类号： C12Q1/533 , C12Q1/18 , G01N33/573 , G01N2021/6439 , G01N2500/00
摘要： This invention comprises a process of isolation and purification of Topoisomerases adsorbed on an immobilized metal affinity chromatography column charged with a metal ions by sequential elution by imidazole solution of different molar strengths. This invention also comprises a novel method of assay of Topoisomerase activity in presence or absence of a Topoisomerase modulator and a kit for same wherein unreacted ScDNA separated from the product of topoisomerase action are removed using a chelating matrix charged with metal ions and fluorescence is reacted with a dye to result in a fluorescent DNA dye complex, and measuring the florescence The assay may be performed in a multi-well plate for assay of Topoisomerase activity, for detecting chemical agents for control of microorganisms or tumours or microbial spoilage of edible products by determining topoisomerase inhibiting or enhancing activity of a potential compound. The dye used may be a cyanine dye.
摘要翻译：本发明包括通过用不同摩尔强度的咪唑溶液进行连续洗脱，分离和纯化吸附在固定的金属亲和层析柱上的拓扑异构酶的方法，该色谱柱装有金属离子。本发明还包括在存在或不存在拓扑异构酶调节剂的情况下测定拓扑异构酶活性的新方法及其用于使用装载有金属离子的螯合基质从拓扑异构酶作用产物分离的未反应ScDNA的试剂盒，并且荧光与产生荧光DNA染料复合物并测量荧光的染料该测定可以在多孔板中进行，用于测定拓扑异构酶活性，用于检测用于控制微生物或肿瘤的化学试剂或通过确定可食用产品的微生物腐败拓扑异构酶抑制或增强潜在化合物的活性。所使用的染料可以是花青染料。

2. 发明申请

WO2010046918A1 PURIFICATION OF SUPERCOILED PLASMID DNA 审中-公开
标题翻译：超滤PLASMID DNA的纯化
公开(公告)号：WO2010046918A1
公开(公告)日：2010-04-29
申请号：PCT/IN2009/000558
申请日：2009-10-08
申请人： V. B. MEDICARE PVT. LTD. , GUPTA, Munishwar, Nath , SINGH, Pradeep, K. , RAGHAVA, Smita
发明人： GUPTA, Munishwar, Nath , SINGH, Pradeep, K. , RAGHAVA, Smita
IPC分类号： C12P19/34 , C12N15/10
CPC分类号： C12N15/1003
摘要： This invention comprises a process of purifying supercoiled plasmid DNA of purity and characteristics required for use in a DNA vaccine or in Gene Therapy or in uses where high purity supercoiled plasmid DNA is required, from a genetically transformed bacterium over-expressing the said supercoiled plasmid and the supercoiled plasmid DNA prepared by a process of this invention and products containing supercoiled DNA made by a process of this invention. The process of this invention comprises selective precipitation from a cell lyste of all DNA and endotoxin except supercoiled DNA by free metal ions. The said impurity comprising plasmid isoforms (linear, relaxed), genomic DNA, RNA, and an endotoxin from a host cell. The method is illustrated by using Cu 2+ metal ions. The said Cu 2+ metal ions were added as CuSO 4 to reach to a final concentration of at least 0.5 M. The removal of metal ions is achieved by dialysis.
摘要翻译：本发明包括从过表达所述超螺旋质粒的遗传转化细菌中纯化在DNA疫苗中或基因治疗或需要高纯度超螺旋质粒DNA的用途中纯化和特征所需的超螺旋质粒DNA的方法，以及通过本发明方法制备的超螺旋质粒DNA和含有本发明方法制备的超螺旋DNA的产物。本发明的方法包括通过游离金属离子从超螺旋DNA除去所有DNA和内毒素的细胞裂解物的选择性沉淀。所述杂质包含来自宿主细胞的质粒同种型（线性，松弛），基因组DNA，RNA和内毒素。该方法通过使用Cu2 +金属离子来说明。将所述Cu 2+金属离子作为CuSO 4加入到达到至少0.5M的终浓度。通过透析去除金属离子。

3. 发明申请

WO2010046920A1 METHODS AND KITS FOR TUNING PERMEABILIZATION OF CELLS BY THREE PHASE PARTITIONING 审中-公开
标题翻译：用于通过三相分级调节细胞渗透的方法和工具
公开(公告)号：WO2010046920A1
公开(公告)日：2010-04-29
申请号：PCT/IN2009/000560
申请日：2009-10-08
申请人： V.B. MEDICARE PVT. LTD. , GUPTA, Munishwar, Nath , RAGHAVA, Smita
发明人： GUPTA, Munishwar, Nath , RAGHAVA, Smita
IPC分类号： C12N1/06 , C12N1/32 , C12Q1/00
CPC分类号： C12N1/06 , C12N1/32 , C12P1/02 , C12P1/04 , C12P21/02 , C12Q1/00 , C12Q1/32
摘要： The invention discloses a process of selective and sequential permeabilization of cells by treating them with a water miscible aliphatic alcohol for a period of time and isolation, purification and recovery of a peptide or/and protein in active form from the permeabilized out components of cells or/and from the permeabilized cells by Three Phase Partitioning to the permeabilized aqueous cell suspension comprising steps of addition of an anti-chaotropic salt to the permeabilized aqueous permeabilized cell suspension to form an aqueous bottom phase, an organic top phase and an intermediate phase between the said lower and top phases. The molecular species of interest or a fraction of the cell of interest is recovered from the appropriate fraction. The said aliphatic alcohol comprises preferably t-butanol, and the said anti-chaotropic salt preferably comprises ammonium sulphate. The peptides and proteins are separated in an active form and with high purity. This invention also comprises a Process of whole cell biocatalysis with high specific activity wherein the permeabilized cells retaining high molecular weight enzymes are used for catalysis.
摘要翻译：本发明公开了一种通过用水混溶性脂肪醇处理一段时间来选择性和顺序地透化细胞的方法，并从细胞的透化组分分离，纯化和回收活性形式的肽或/和蛋白质，或并且通过三相分离从透化细胞到渗透的水性细胞悬浮液，包括以下步骤：向透化的透水化细胞悬浮液中加入抗离液序列盐以形成水相底部相，有机顶相和中间相表示较低和最高阶段。从适当的级分回收感兴趣的分子物质或目的细胞的一部分。所述脂族醇优选包含叔丁醇，所述抗离液序列盐优选包含硫酸铵。肽和蛋白质以活性形式和高纯度分离。本发明还包括具有高比活性的全细胞生物催化过程，其中将保留高分子量酶的透化细胞用于催化。

4. 发明申请

WO2010046919A2 A STEREOSELECTIVE ENZYME ALCOHOL DEHYDROGENASE CONVERTING GLYCEROL TO D-GLYCERALDEHYDE: EXTRACTION, PURIFICATION AND CHARACTERIZATION 审中-公开
标题翻译：立体选择性乙醇脱氢酶将甘油转化为D-甘油醛：萃取，纯化和表征
公开(公告)号：WO2010046919A2
公开(公告)日：2010-04-29
申请号：PCT/IN2009/000559
申请日：2009-10-08
申请人： V.B. MEDICARE PVT. LTD. , GUPTA, Munishwar, Nath , RAGHAVA, Smita
发明人： GUPTA, Munishwar, Nath , RAGHAVA, Smita
CPC分类号： C12P7/24 , C12N9/0006
摘要： This invention discloses an Isolated thermostable alcohol dehydrogenase enzyme isolated from Thermus thermophilus having high stereospecific activity towards glycerol and butanol as compared to short chain and secondary alcohols. And a process of[ isolating the said enzyme on an adsorbent by using ammonium sulphate gradient elution. This invention also discloses a method of producing optically active glyceraldehyde from glycerol.
摘要翻译：与短链醇和仲醇相比，本发明公开了从Thermus thermophilus分离的分离的耐热醇脱氢酶，其对甘油和丁醇具有高立体特异性活性。 [通过使用硫酸铵梯度洗脱将所述酶分离到吸附剂上的过程。本发明还公开了一种由甘油生产光学活性甘油醛的方法。

5. 发明申请

WO2018204374A1 FORMULATIONS OF ANTI-LAG3 ANTIBODIES AND CO-FORMULATIONS OF ANTI-LAG3 ANTIBODIES AND ANTI-PD-1 ANTIBODIES 审中-公开
公开(公告)号：WO2018204374A1
公开(公告)日：2018-11-08
申请号：PCT/US2018/030468
申请日：2018-05-01
申请人： MERCK SHARP & DOHME CORP. , DESAI, Preeti, G. , SHI, Shuai , ANTOCHSHUK, Valentyn , BURLAGE, Rubi , RAGHAVA, Smita
发明人： DESAI, Preeti, G. , SHI, Shuai , ANTOCHSHUK, Valentyn , BURLAGE, Rubi , RAGHAVA, Smita
IPC分类号： A61K39/395 , C07K16/00 , C07K16/28 , C07K16/46 , C07K19/00 , C12P21/08
摘要： The present invention provides formulations of anti-LAG3 antibodies, and co-formulations of anti-PD-1 antibodies and anti-LAG3 antibodies, and their use in treating various disorders.

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