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1. 发明申请

US20100062505A1 BUTANOL PRODUCTION BY METABOLICALLY ENGINEERED YEAST 审中-公开
标题翻译：丁醇生产由代谢工程的YEAST
公开(公告)号：US20100062505A1
公开(公告)日：2010-03-11
申请号：US11963542
申请日：2007-12-21
申请人： Uvini Gunawardena , Peter Meinhold , Matthew W. Peters , Jun Urano , Reid M. Renny Feldman
发明人： Uvini Gunawardena , Peter Meinhold , Matthew W. Peters , Jun Urano , Reid M. Renny Feldman
IPC分类号： C12P7/16 , C12N1/18 , C12N1/15
CPC分类号： C12P7/16 , C12N9/0008 , C12N9/88 , C12N9/93 , Y02E50/10
摘要： There are disclosed metabolically-engineered yeast and methods of producing n-butanol. In an embodiment, metabolically-engineered yeast is capable of metabolizing a carbon source to produce n-butanol, at least one pathway produces increased cytosolic acetyl-CoA relative to cytosolic acetyl-CoA produced by a wild-type yeast, and at least one heterologous gene encodes and expresses at least one enzyme for a metabolic pathway capable of utilizing NADH to convert acetyl-CoA to n-butanol. In another embodiment, a method of producing n-butanol includes (a) providing metabolically-engineered yeast capable of metabolizing a carbon source to produce n-butanol, at least one pathway produces increased cytosolic acetyl-CoA relative to cytosolic acetyl-CoA produced by a wild-type yeast, and at least one heterologous gene encodes and expresses at least one enzyme for a metabolic pathway utilizing NADH to convert acetyl-CoA to n-butanol; and (b) culturing the yeast to produce n-butanol. Other embodiments are also disclosed.
摘要翻译：公开了代谢工程酵母和生产正丁醇的方法。在一个实施方案中，经代谢工程化的酵母能够代谢碳源以产生正丁醇，相对于由野生型酵母产生的胞质乙酰辅酶A，至少一种途径产生增加的胞质乙酰辅酶A，并且至少一种异源基因编码并表达至少一种能够利用NADH将乙酰辅酶A转化为正丁醇的代谢途径的酶。在另一个实施方案中，生产正丁醇的方法包括（a）提供能够代谢碳源以产生正丁醇的经代谢工程化的酵母，至少一个途径相对于由胞质乙酰辅酶A产生的细胞溶质乙酰辅酶A产生增加的细胞溶质乙酰辅酶A 野生型酵母和至少一种异源基因编码并表达至少一种利用NADH将乙酰辅酶A转化为正丁醇的代谢途径的酶; 和（b）培养酵母以生产正丁醇。还公开了其他实施例。

2. 发明授权

US08017375B2 Yeast organism producing isobutanol at a high yield 无效
标题翻译：以高产率生产异丁醇的酵母生物
公开(公告)号：US08017375B2
公开(公告)日：2011-09-13
申请号：US12343375
申请日：2008-12-23
申请人： Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano , Peter Meinhold , Aristos A. Aristidou , Catherine Asleson Dundon , Christopher Smith
发明人： Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano , Peter Meinhold , Aristos A. Aristidou , Catherine Asleson Dundon , Christopher Smith
IPC分类号： C12P7/04 , C12P7/16 , C12N1/15 , C12N1/16 , C12N15/00 , C12N9/88
CPC分类号： C12N9/88 , C12P7/16 , C12Y401/01001 , Y02E50/10
摘要： There is disclosed a method of producing isobutanol. In an embodiment, the method includes providing a microorganism transformed with an isobutanol producing pathway containing at least one exogenous gene. The microorganism is selected to produce isobutanol from a carbon source at a yield of at least 10 percent theoretical. The method includes cultivating the microorganism in a culture medium containing a feedstock providing the carbon source, until isobutanol is produced. The method includes recovering the isobutanol. In one embodiment, the microorganism is a yeast with a Crabtree-negative phenotype. In another embodiment, the microorganism is a yeast microorganism with a Crabtree-positive phenotype. There is disclosed a microorganism for producing isobutanol. In an embodiment, the microorganism includes an isobutanol producing pathway containing at least one exogenous gene, and is selected to produce a recoverable quantity of isobutanol from a carbon source at a yield of at least 10 percent theoretical.
摘要翻译：公开了异丁醇的制造方法。在一个实施方案中，该方法包括提供转化了含有至少一种外源基因的产生异丁醇的途径的微生物。选择微生物以产生至少10％的理论值从碳源产生异丁醇。该方法包括在含有提供碳源的原料的培养基中培养微生物，直到产生异丁醇。该方法包括回收异丁醇。在一个实施方案中，微生物是具有Crabtree-阴性表型的酵母。在另一个实施方案中，微生物是具有Crabtree阳性表型的酵母微生物。公开了用于生产异丁醇的微生物。在一个实施方案中，微生物包括含有至少一种外源基因的异丁醇生产途径，并且选择其以至少10％理论值的产率从碳源产生可回收量的异丁醇。

3. 发明授权

US08614077B2 Recovery of higher alcohols from dilute aqueous solutions 有权
标题翻译：从稀水溶液中回收高级醇
公开(公告)号：US08614077B2
公开(公告)日：2013-12-24
申请号：US13302775
申请日：2011-11-22
申请人： William A. Evanko , Aharon M. Eyal , David A. Glassner , Fudu Miao , Aristos A. Aristidou , Kent Evans , Patrick R. Gruber , Andrew C. Hawkins , Peter Meinhold , Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano
发明人： William A. Evanko , Aharon M. Eyal , David A. Glassner , Fudu Miao , Aristos A. Aristidou , Kent Evans , Patrick R. Gruber , Andrew C. Hawkins , Peter Meinhold , Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano
IPC分类号： C12P7/16 , C12N9/10 , C12P17/10
CPC分类号： C12M43/00 , C07C29/80 , C07C29/86 , C12C11/11 , C12M45/20 , C12P7/04 , C12P7/16 , Y02E50/10 , Y02E50/17 , Y10T29/49826 , C07C31/12
摘要： This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allows recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs.
摘要翻译：本发明涉及从稀释的水溶液如发酵液中回收C3-C6醇的方法。这样的方法提供了用于发酵的改进的体积生产率并允许酒精回收。由于通过同时发酵和回收过程增加了醇产物的有效浓度，所以这种方法还允许在用过的发酵液的生产和干燥中减少能量消耗，这增加了每一批发酵液干燥产生和回收的醇的量。因此，本发明允许以低资本生产和回收C3-C6醇，降低运行成本。

4. 发明授权

US08455239B2 Yeast organism producing isobutanol at a high yield 有权
标题翻译：以高产率生产异丁醇的酵母生物
公开(公告)号：US08455239B2
公开(公告)日：2013-06-04
申请号：US12820505
申请日：2010-06-22
申请人： Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano , Peter Meinhold , Aristos Aristidou , Catherine Asleson Dundon , Christopher Smith
发明人： Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano , Peter Meinhold , Aristos Aristidou , Catherine Asleson Dundon , Christopher Smith
IPC分类号： C12P7/04 , C12P7/16 , C12N1/15 , C12N1/16 , C12N15/00 , C12N9/88
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N9/1022 , C12N9/88 , C12N9/90 , C12N15/81 , C12Y101/01001 , C12Y101/01008 , C12Y101/01086 , C12Y101/05003 , C12Y202/01006 , C12Y401/01001 , C12Y401/01074 , C12Y402/01009 , Y02E50/10
摘要： The present invention provides recombinant mircoorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译：本发明提供包含产生异丁醇的代谢途径的重组微生物和使用所述重组微生物产生异丁醇的方法。在本发明的各个方面，重组微生物可以包含导致丙酮酸脱羧酶和/或甘油-3-磷酸脱氢酶活性降低的修饰。在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物，和非发酵酵母微生物。

5. 发明申请

US20110318799A1 YEAST ORGANISM PRODUCING ISOBUTANOL AT A HIGH YIELD 审中-公开
标题翻译： YEAST ORGANISM在高产量下生产ISOBUTANOL
公开(公告)号：US20110318799A1
公开(公告)日：2011-12-29
申请号：US13229438
申请日：2011-09-09
申请人： Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano , Peter Meinhold , Aristos A. Aristidou , Catherine Asleson Dundon , Christopher Smith
发明人： Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano , Peter Meinhold , Aristos A. Aristidou , Catherine Asleson Dundon , Christopher Smith
IPC分类号： C12P7/16
CPC分类号： C12N9/88 , C12P7/16 , C12Y401/01001 , Y02E50/10
摘要： There is disclosed a method of producing isobutanol. In an embodiment, the method includes providing a microorganism transformed with an isobutanol producing pathway containing at least one exogenous gene. The microorganism is selected to produce isobutanol from a carbon source at a yield of at least 10 percent theoretical. The method includes cultivating the microorganism in a culture medium containing a feedstock providing the carbon source, until isobutanol is produced. The method includes recovering the isobutanol. In one embodiment, the microorganism is a yeast with a Crabtree-negative phenotype. In another embodiment, the microorganism is a yeast microorganism with a Crabtree-positive phenotype. There is disclosed a microorganism for producing isobutanol. In an embodiment, the microorganism includes an isobutanol producing pathway containing at least one exogenous gene, and is selected to produce a recoverable quantity of isobutanol from a carbon source at a yield of at least 10 percent theoretical.
摘要翻译：公开了异丁醇的制造方法。在一个实施方案中，该方法包括提供转化了含有至少一种外源基因的产生异丁醇的途径的微生物。选择微生物以产生至少10％的理论值从碳源产生异丁醇。该方法包括在含有提供碳源的原料的培养基中培养微生物，直到产生异丁醇。该方法包括回收异丁醇。在一个实施方案中，微生物是具有Crabtree-阴性表型的酵母。在另一个实施方案中，微生物是具有Crabtree阳性表型的酵母微生物。公开了用于生产异丁醇的微生物。在一个实施方案中，微生物包括含有至少一种外源基因的异丁醇生产途径，并且选择其以至少10％理论值的产率从碳源产生可回收量的异丁醇。

6. 发明申请

US20110183392A1 YEAST ORGANISM PRODUCING ISOBUTANOL AT A HIGH YIELD 审中-公开
公开(公告)号：US20110183392A1
公开(公告)日：2011-07-28
申请号：US12696645
申请日：2010-01-29
申请人： Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano , Peter Meinhold , Aristos Aristidou , Catherine Asleson Dundon , Christopher Smith
发明人： Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano , Peter Meinhold , Aristos Aristidou , Catherine Asleson Dundon , Christopher Smith
IPC分类号： C12P7/16 , C12N1/19
CPC分类号： C12N9/88 , C12P7/16 , C12Y401/01001 , Y02E50/10
摘要： There is disclosed a method of producing isobutanol. In an embodiment, the method includes providing a microorganism transformed with an isobutanol producing pathway containing at least one exogenous gene. The microorganism is selected to produce isobutanol from a carbon source at a yield of at least 10 percent theoretical. The method includes cultivating the microorganism in a culture medium containing a feedstock providing the carbon source, until isobutanol is produced. The method includes recovering the isobutanol. In one embodiment, the microorganism is a yeast with a Crabtree-negative phenotype. In another embodiment, the microorganism is a yeast microorganism with a Crabtree-positive phenotype. There is disclosed a microorganism for producing isobutanol. In an embodiment, the microorganism includes an isobutanol producing pathway containing at least one exogenous gene, and is selected to produce a recoverable quantity of isobutanol from a carbon source at a yield of at least 10 percent theoretical.

7. 发明申请

US20130183730A9 RECOVERY OF HIGHER ALCOHOLS FROM DILUTE AQUEOUS SOLUTIONS 有权
公开(公告)号：US20130183730A9
公开(公告)日：2013-07-18
申请号：US13302775
申请日：2011-11-22
申请人： William A. Evanko , Aharon M. Eyal , David A. Glassner , Fudu Miao , Aristos A. Aristidou , Kent Evans , Patrick R. Gruber , Andrew C. Hawkins , Peter Meinhold , Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano
发明人： William A. Evanko , Aharon M. Eyal , David A. Glassner , Fudu Miao , Aristos A. Aristidou , Kent Evans , Patrick R. Gruber , Andrew C. Hawkins , Peter Meinhold , Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano
IPC分类号： C12P7/16
CPC分类号： C12M43/00 , C07C29/80 , C07C29/86 , C12C11/11 , C12M45/20 , C12P7/04 , C12P7/16 , Y02E50/10 , Y02E50/17 , Y10T29/49826 , C07C31/12
摘要： This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allows recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs.

8. 发明申请

US20120028323A1 YEAST ORGANISM PRODUCING ISOBUTANOL AT A HIGH YIELD 审中-公开
标题翻译： YEAST ORGANISM在高产量下生产ISOBUTANOL
公开(公告)号：US20120028323A1
公开(公告)日：2012-02-02
申请号：US13267771
申请日：2011-10-06
申请人： Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano , Peter Meinhold , Aristos Aristidou , Catherine Asleson Dundon , Christopher Smith
发明人： Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano , Peter Meinhold , Aristos Aristidou , Catherine Asleson Dundon , Christopher Smith
IPC分类号： C12P7/16 , C12N1/19
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N9/1022 , C12N9/88 , C12N9/90 , C12N15/81 , C12Y101/01001 , C12Y101/01008 , C12Y101/01086 , C12Y101/05003 , C12Y202/01006 , C12Y401/01001 , C12Y401/01074 , C12Y402/01009 , Y02E50/10
摘要： The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译：本发明提供包含产生异丁醇的代谢途径的重组微生物和使用所述重组微生物产生异丁醇的方法。在本发明的各个方面，重组微生物可以包含导致丙酮酸脱羧酶和/或甘油-3-磷酸脱氢酶活性降低的修饰。在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物，和非发酵酵母微生物。

9. 发明申请

US20110020889A1 YEAST ORGANISM PRODUCING ISOBUTANOL AT A HIGH YIELD 有权
标题翻译： YEAST ORGANISM在高产量下生产ISOBUTANOL
公开(公告)号：US20110020889A1
公开(公告)日：2011-01-27
申请号：US12820505
申请日：2010-06-22
申请人： Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano , Peter Meinhold , Aristos Aristidou , Catherine Asleson Dundon , Christopher Smith
发明人： Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano , Peter Meinhold , Aristos Aristidou , Catherine Asleson Dundon , Christopher Smith
IPC分类号： C12P7/16 , C12N1/19
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N9/1022 , C12N9/88 , C12N9/90 , C12N15/81 , C12Y101/01001 , C12Y101/01008 , C12Y101/01086 , C12Y101/05003 , C12Y202/01006 , C12Y401/01001 , C12Y401/01074 , C12Y402/01009 , Y02E50/10
摘要： The present invention provides recombinant mircoorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译：本发明提供包含产生异丁醇的代谢途径的重组微生物和使用所述重组微生物产生异丁醇的方法。在本发明的各个方面，重组微生物可以包含导致丙酮酸脱羧酶和/或甘油-3-磷酸脱氢酶活性降低的修饰。在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物，和非发酵酵母微生物。

10. 发明申请

US20120190089A1 ENGINEERED MICROOGRANISMS CAPABLE OF PRODUCING TARGET COMPOUNDS UNDER ANAEROBIC CONDITIONS 审中-公开
标题翻译：在无机条件下生产目标化合物的工程化微生物
公开(公告)号：US20120190089A1
公开(公告)日：2012-07-26
申请号：US13303884
申请日：2011-11-23
申请人： Thomas Buelter , Peter Meinhold , Reid M. Renny Feldman , Andrew C. Hawkins , Sabine Bastian , Frances Arnold , Jun Urano
发明人： Thomas Buelter , Peter Meinhold , Reid M. Renny Feldman , Andrew C. Hawkins , Sabine Bastian , Frances Arnold , Jun Urano
IPC分类号： C12P7/16 , C12N1/19
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N9/0036 , Y02E50/10
摘要： The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.
摘要翻译：本发明通常提供重组微生物，其包含能够在需氧和厌氧条件下生产C3-C5醇的工程化代谢途径。本发明还提供已被突变或修饰以提高其NADH依赖性活性或将辅因子偏好从NADPH转变为NADH并在修饰的微生物中表达的酮酸酸还原异构酶。此外，本发明提供以修饰微生物表达的异丁醛脱氢酶。还提供了在需氧和厌氧条件下通过使合适的底物与本发明的改性微生物接触来生产有益代谢物的方法。

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