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1. 发明授权

US08604278B2 Expression cassettes for transgenic expression of nucleic acids 失效
标题翻译：用于核酸转基因表达的表达盒
公开(公告)号：US08604278B2
公开(公告)日：2013-12-10
申请号：US13113615
申请日：2011-05-23
申请人： Ute Heim , Helke Hillebrand , Irene Kunze , Karin Herbers , Uwe Sonnewald , Eric Glickmann , Wolfgang Lein , Rüdiger Hell , Ricarda Jost
发明人： Ute Heim , Helke Hillebrand , Irene Kunze , Karin Herbers , Uwe Sonnewald , Eric Glickmann , Wolfgang Lein , Rüdiger Hell , Ricarda Jost
IPC分类号： A01H5/00 , C12N15/63 , C12N15/82 , C12N15/87 , C12N5/14 , C07H21/00 , C07H21/04
CPC分类号： C12N15/8216
摘要： The invention relates to expression cassettes and vectors which contain constitutive promoters of plants and to the use of these expression cassettes or vectors for transgenic expression of nucleic acid sequences, preferably selection markers, in organisms, preferably in plants. The invention further relates to transgenic plants which have been transformed with the expression cassettes or vectors, to cultures, parts or propagation material derived from these plants, and to the use of these plants for the production of food and animal feedstuffs, seed, pharmaceuticals, or fine chemicals.
摘要翻译：本发明涉及含有植物的组成型启动子的表达盒和载体，以及这些表达盒或载体用于在生物体，优选植物中的核酸序列，优选选择标记的转基因表达的用途。本发明还涉及已经用表达盒或载体转化的转基因植物，衍生自这些植物的培养物，部分或繁殖材料，以及这些植物用于生产食品和动物饲料，种子，药物，或精细化学品。

2. 发明授权

US08022272B2 Expression cassettes for transgenic expression of nucleic acids 有权
标题翻译：用于核酸转基因表达的表达盒
公开(公告)号：US08022272B2
公开(公告)日：2011-09-20
申请号：US10755677
申请日：2004-01-13
申请人： Ute Heim , Helke Hillebrand , Irene Kunze , Karin Herbers , Uwe Sonnewald , Eric Glickmann , Wolfgang Lein , Rüdiger Hell , Ricarda Jost
发明人： Ute Heim , Helke Hillebrand , Irene Kunze , Karin Herbers , Uwe Sonnewald , Eric Glickmann , Wolfgang Lein , Rüdiger Hell , Ricarda Jost
IPC分类号： A01H5/00 , C12N15/82 , C12N15/87 , C12N5/14 , C12N15/63 , C07H21/04 , C07H21/00
CPC分类号： C12N15/8216
摘要： The invention relates to expression cassettes and vectors, which contain vegetable constitutive promoters and to the use of these expression cassettes or vectors for the transgenic expression of nucleic acid sequences preferably selection markers in organisms, preferably in plants. The invention also relates to transgenic plants that have been transformed using these expression cassettes or vectors, to cultures, parts or propagation products derived from these plants, and to the use of these plants for producing food and animal feed agents, seeds, pharmaceuticals, or fine chemicals.
摘要翻译：本发明涉及含有植物组成型启动子的表达盒和载体，以及这些表达盒或载体用于转基因表达核酸序列的优点，优选在生物体中，优选在植物中的选择标记。本发明还涉及已经使用这些表达盒或载体转化的转基因植物，衍生自这些植物的培养物，部分或繁殖产物，以及这些植物用于生产食物和动物饲料，种子，药物或精细化学品。

3. 发明申请

US20110289622A1 EXPRESSION CASSETTES FOR TRANSGENIC EXPRESSION OF NUCLEIC ACIDS 失效
标题翻译：用于转基因表达核酸的表达载体
公开(公告)号：US20110289622A1
公开(公告)日：2011-11-24
申请号：US13113615
申请日：2011-05-23
申请人： Ute Heim , Helke Hillebrand , Irene Kunze , Karin Herbers , Uwe Sonnewald , Eric Glickmann , Wolfgang Lein , Rüdiger Hell , Ricarda Jost
发明人： Ute Heim , Helke Hillebrand , Irene Kunze , Karin Herbers , Uwe Sonnewald , Eric Glickmann , Wolfgang Lein , Rüdiger Hell , Ricarda Jost
IPC分类号： A01H1/00 , C12N9/00 , C12N5/10 , C12N1/21 , A01H5/00 , C12N1/19 , C12N15/63 , C07K14/00 , C07K16/00 , A01K67/027 , C12Q1/68 , C12N1/15
CPC分类号： C12N15/8216
摘要： The invention relates to expression cassettes and vectors which contain constitutive promoters of plants and to the use of these expression cassettes or vectors for transgenic expression of nucleic acid sequences, preferably selection markers, in organisms, preferably in plants. The invention further relates to transgenic plants which have been transformed with the expression cassettes or vectors, to cultures, parts or propagation material derived from these plants, and to the use of these plants for the production of food and animal feedstuffs, seed, pharmaceuticals, or fine chemicals.
摘要翻译：本发明涉及含有植物的组成型启动子的表达盒和载体，以及这些表达盒或载体用于在生物体，优选植物中的核酸序列，优选选择标记的转基因表达的用途。本发明还涉及已经用表达盒或载体转化的转基因植物，衍生自这些植物的培养物，部分或繁殖材料，以及这些植物用于生产食品和动物饲料，种子，药物，或精细化学品。

4. 发明申请

US20050216967A1 Expression cassettes for transgenic expression of nucleic acids 有权
标题翻译：用于核酸转基因表达的表达盒
公开(公告)号：US20050216967A1
公开(公告)日：2005-09-29
申请号：US10755677
申请日：2004-01-13
申请人： Ute Heim , Helke Hillebrand , Irene Kunze , Karin Herbers , Uwe Sonnewald , Eric Glickmann , Wolfgang Lein , Rudiger Hell , Ricarda Jost
发明人： Ute Heim , Helke Hillebrand , Irene Kunze , Karin Herbers , Uwe Sonnewald , Eric Glickmann , Wolfgang Lein , Rudiger Hell , Ricarda Jost
IPC分类号： A01H1/00 , C12N15/82
CPC分类号： C12N15/8216
摘要： The invention relates to expression cassettes and vectors which contain constitutive promoters of plants and to the use of said expression cassettes or vectors for transgenic expression of nucleic acid sequences, preferably selection markers, in organisms, preferably in plants. The invention further relates to transgenic plants which have been transformed with said expression cassettes or vectors, to cultures, parts or propagation material derived therefrom and also to the use of same for the production of food- and feedstuffs, seed, pharmaceuticals or fine chemicals.
摘要翻译：本发明涉及含有植物组成型启动子的表达盒和载体，以及使用所述表达盒或载体用于在生物体，优选植物中的核酸序列，优选选择标记的转基因表达。本发明还涉及已经用所述表达盒或载体转化的转基因植物，从其衍生的培养物，部分或繁殖材料，以及用于生产食品和饲料，种子，药物或精细化学品的转基因植物。

5. 发明授权

US07303909B2 Binary vectors for the improved transformation of plants systems 失效
标题翻译：用于改进植物系统转化的二元载体
公开(公告)号：US07303909B2
公开(公告)日：2007-12-04
申请号：US10311885
申请日：2001-06-28
申请人： Ute Heim , Karin Herbers , Irene Kunze
发明人： Ute Heim , Karin Herbers , Irene Kunze
IPC分类号： C12N15/82 , C12N15/84
CPC分类号： C12N15/8205
摘要： The present invention relates to a binary vector which is distinguished by the combination of a variety of elements, demonstrates particularly high compatibility from the cloning aspect and which, besides the T-DNA flanked by the right and left border, contains an additional sequence which makes possible highly efficient and correct transfer of the T-DNA.
摘要翻译：本发明涉及通过多种元素的组合区分的二元载体，表现出克隆方面特别高的相容性，以及除了右侧和左侧边界两侧的T-DNA之外，还包含另外的序列，其使得可能的高效和正确的T-DNA转移。

6. 发明申请

US20060253927A1 Method for the production of zeaxanthin and/or biosynthetic intermediates and/or subsequent products thereof 审中-公开
标题翻译：用于生产玉米黄质和/或生物合成中间体的方法和/或其后续产物
公开(公告)号：US20060253927A1
公开(公告)日：2006-11-09
申请号：US10524652
申请日：2003-08-18
申请人： Christel Schopfer , Ralf Flachmann , Karin Herbers , Irene Kunze , Matt Sauer , Martin Klebsattel
发明人： Christel Schopfer , Ralf Flachmann , Karin Herbers , Irene Kunze , Matt Sauer , Martin Klebsattel
IPC分类号： A01H1/00 , A01H5/00 , C12P23/00 , C12N15/82 , C12N5/04
CPC分类号： C12N9/0069 , A23K10/30 , A23K20/179 , A23K50/80 , A23L5/44 , A23L33/155 , A23V2002/00 , C07H21/00 , C09B61/00 , C12N9/0004 , C12N15/823 , C12N15/8243 , C12N15/825 , C12P23/00 , Y02A40/818 , A23V2300/21
摘要： The present invention relates to a process for preparing zeaxanthin and/or biosynthetic intermediates and/or secondary products thereof by culturing genetically modified plants which, compared to the wild type, have a reduced ε-cyclase activity caused by double-stranded ε-cyclase ribonucleic acid sequences, to the genetically modified plants and to the use thereof as foodstuffs and feedstuffs and for producing carotenoid extracts.
摘要翻译：本发明涉及通过培养与野生型相比具有由双链ε-环化酶核糖核酸引起的ε-环化酶活性降低的遗传修饰植物来制备玉米黄质和/或其生物合成中间体和/或其次级产物的方法酸序列，转基因植物及其作为食品和饲料以及用于生产类胡萝卜素提取物的用途。

7. 发明授权

US07557203B2 Expression cassettes for the bi-directional transgenic expression of nucleic acids in plants 失效
标题翻译：用于植物中核酸双向转录表达的表达盒
公开(公告)号：US07557203B2
公开(公告)日：2009-07-07
申请号：US10565221
申请日：2004-07-03
申请人： Ute Linemann , Karin Herbers , Irene Kunze
发明人： Ute Linemann , Karin Herbers , Irene Kunze
IPC分类号： C12N15/82 , C12N15/00 , A01H5/00 , A01H5/10 , C12N15/11 , C12N15/63
CPC分类号： C12N15/8216
摘要： The invention relates to transgenic expression cassettes for expressing two nucleic acid sequences in a plant cell comprising at least one regulatory sequence selected from the group consisting of a) the promoter shown in SEQ ID NO: 1 or 2, b) functional equivalents of the promoter shown in SEQ ID NO: 1 or 2 which have an identity of at least 80% to the sequence shown in SEQ ID NO: 1 or 2 and which have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, c) functional equivalents of the promoter shown in SEQ ID NO: 1 or 2 which comprise at least 25 consecutive nucleotides of the sequences shown in SEQ ID NO: 1 or 2 and which have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, and d) functionally equivalent fragments of sequences a) or b) or c), which have at least 25 consecutive nucleotides of said sequences a) or b) or c) and have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, where said regulatory element is disposed between two nucleic acid sequences and is heterogeneous in relation to said nucleic acid sequences and is functionally linked to said nucleic acid sequences in such a way that the expression of two different ribonucleic acid sequences is brought about in at least one plant cell, where said ribonucleic acid sequences are selected from ribonucleic acid sequences coding for i) amino acid sequences or ii) ribonucleic acid sequences which bring about a reduction in the expression of at least one endogenous gene of said plant cell.
摘要翻译：本发明涉及用于在植物细胞中表达两种核酸序列的转基因表达盒，其包含至少一种选自a）SEQ ID NO：1或2所示的启动子的调节序列，b）启动子的功能等同物如SEQ ID NO：1或2所示，其与SEQ ID NO：1或2所示的序列具有至少80％的同一性，并且与SEQ ID NO：1或2所示的启动子具有基本上相同的启动子活性，c）SEQ ID NO：1或2所示的启动子的功能等同物，其包含SEQ ID NO：1或2所示序列的至少25个连续核苷酸并且与SEQ ID NO：1或2所示的启动子具有基本上相同的启动子活性 SEQ ID NO：1或2，以及d）序列a）或b）或c）的功能上等同的片段，其具有所述序列a）或b）或c）的至少25个连续核苷酸，并且具有基本上相同的启动子活性 SEQ ID NO：1或2所示的启动子，其中所述调节元件设置在两个核酸序列之间并且与所述核酸序列相关并且与所述核酸序列功能性连接，使得两个不同的核糖核酸序列的表达在至少一个植物细胞，其中所述核糖核酸序列选自编码i）氨基酸序列的核糖核酸序列或ii）导致所述植物细胞的至少一个内源基因表达降低的核糖核酸序列。

8. 发明申请

US20070094749A1 Method for producing ketocarotinoids in plant fruit 审中-公开
标题翻译：植物果实中生酮酮类的方法
公开(公告)号：US20070094749A1
公开(公告)日：2007-04-26
申请号：US10524829
申请日：2003-08-18
申请人： Christel Schopfer , Ralf Flachmann , Karin Herbers , Irene Kunze , Matt Sauer , Martin Klebsattel
发明人： Christel Schopfer , Ralf Flachmann , Karin Herbers , Irene Kunze , Matt Sauer , Martin Klebsattel
IPC分类号： A01H1/00 , C12P23/00 , C12N15/82 , C12N5/04
CPC分类号： C12N9/0069 , A23K10/30 , A23K20/179 , A23K50/80 , A23L5/44 , A23L33/155 , A23V2002/00 , C07H21/00 , C09B61/00 , C12N9/0004 , C12N15/823 , C12N15/8243 , C12N15/825 , C12P23/00 , Y02A40/818 , A23V2300/21
摘要： The invention relates to a method for the production of ketocarotenoids by culturing genetically modified plants which show a ketolase activity in fruits.
摘要翻译：本发明涉及通过培养在果实中显示酮糖酶活性的经遗传修饰的植物生产酮类胡萝卜素的方法。

9. 发明申请

US20060150282A1 Expression cassettes for the bi-directional transgenic expression of nucleic acids in plants 失效
公开(公告)号：US20060150282A1
公开(公告)日：2006-07-06
申请号：US10565221
申请日：2004-07-03
申请人： Ute Linemann , Karin Herbers , Irene Kunze
发明人： Ute Linemann , Karin Herbers , Irene Kunze
IPC分类号： C12N5/04 , C12N15/82 , A01H1/00
CPC分类号： C12N15/8216
摘要： The invention relates to transgenic expression cassettes for expressing two nucleic acid sequences in a plant cell comprising at least one regulatory sequence selected from the group consisting of a) the promoter shown in SEQ ID NO: 1 or 2, b) functional equivalents of the promoter shown in SEQ ID NO: 1 or 2 which have an identity of at least 80% to the sequence shown in SEQ ID NO: 1 or 2 and which have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, c) functional equivalents of the promoter shown in SEQ ID NO: 1 or 2 which comprise at least 25 consecutive nucleotides of the sequences shown in SEQ ID NO: 1 or 2 and which have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, and d) functionally equivalent fragments of sequences a) or b) or c), which have at least 25 consecutive nucleotides of said sequences a) or b) or c) and have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, where said regulatory element is disposed between two nucleic acid sequences and is heterogeneous in relation to said nucleic acid sequences and is functionally linked to said nucleic acid sequences in such a way that the expression of two different ribonucleic acid sequences is brought about in at least one plant cell, where said ribonucleic acid sequences are selected from ribonucleic acid sequences coding for i) amino acid sequences or ii) ribonucleic acid sequences which bring about a reduction in the expression of at least one endogenous gene of said plant cell.

10. 发明申请

US20060031963A1 Method for the production of ketocarotinoids in flower petals on plants 审中-公开
标题翻译：在植物花瓣上生产酮类动物的方法
公开(公告)号：US20060031963A1
公开(公告)日：2006-02-09
申请号：US10524972
申请日：2003-08-18
申请人： Christel Schopfer , Ralf Flachmann , Karin Herbers , Irene Kunze , Matt Sauer , Martin Klebsattel
发明人： Christel Schopfer , Ralf Flachmann , Karin Herbers , Irene Kunze , Matt Sauer , Martin Klebsattel
IPC分类号： A01H1/00 , C12P23/00 , C12N15/82 , C12N5/04
CPC分类号： C12N9/0069 , A23K10/30 , A23K20/179 , A23K50/80 , A23L5/44 , A23L33/155 , A23V2002/00 , C07H21/00 , C09B61/00 , C12N9/0004 , C12N15/823 , C12N15/8243 , C12N15/825 , C12P23/00 , Y02A40/818 , A23V2300/21
摘要： The present invention relates to a method for the production of ketocarotenoids by culturing plants which show a modified ketolase activity in petals in comparison with the wild type, to the genetically modified plants, to their use as foodstuffs and feedstuffs, and for the production of ketocarotenoid extracts.
摘要翻译：本发明涉及一种生产酮类胡萝卜素的方法，所述方法通过培养与野生型相比在花瓣中显示出改变的酮醇酶活性，转基因植物，用作食品和饲料，以及生产酮类胡萝卜素提取物。

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