专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明专利

JP2006158401A Culture method for compositions containing human stem cells and method for transformation 审中-公开
标题翻译：包含人干细胞的组合物的培养方法和转化方法
公开(公告)号：JP2006158401A
公开(公告)日：2006-06-22
申请号：JP2006000956
申请日：2006-01-05
申请人： Univ Michigan , リージェンツオブザユニバーシティオブミシガンRegents Of The University Of Michigan
发明人： EMERSON STEPHEN G , CLARKE MICHAEL F , PALSSON BERNHARD O , SCHWARTZ RICHARD M
IPC分类号： A61K35/28 , A61K48/00 , A61P3/10 , A61P7/06 , A61P9/12 , A61P25/28 , A61P31/18 , A61P35/00 , C07K14/535 , C07K14/54 , C07K14/705 , C12M1/34 , C12M3/00 , C12M3/02 , C12M3/06 , C12N5/02 , C12N5/078 , C12N5/0787 , C12N5/0789 , C12N5/10 , C12N15/09 , C12N15/85 , C12N15/867
CPC分类号： C12N15/86 , A61K48/00 , C07K14/535 , C07K14/5403 , C07K14/5412 , C07K14/705 , C12M23/34 , C12M29/04 , C12M29/10 , C12M35/08 , C12N5/0641 , C12N5/0642 , C12N5/0647 , C12N15/85 , C12N2501/125 , C12N2501/14 , C12N2501/22 , C12N2501/23 , C12N2501/39 , C12N2502/13 , C12N2502/1394 , C12N2502/99 , C12N2740/13043 , C12N2830/002 , C12N2830/85
摘要： PROBLEM TO BE SOLVED: To provide a novel method, including culture media conditions, reactors, etc., for ex vivo human stem cell proliferation and transformation of stable genes, as well as a novel method, including culture media conditions and reactors, for optimum conditions for (i) culture of human hematopoietic precursor cells and (ii) transformation of stable genes of human hematopoietic precursor cells. SOLUTION: Disclosed is a culture method for obtaining ex vivo human stem cell division products derived from hepatopoietic system featured by that cell compositions including human hepatopoietic system-derived stem cells are cultured in a liquid culture medium which is continuously or periodically replaced at a rate of 1 mL of medium per 1 mL of culture for about 24 to about 48 hours, metabolic products are removed while maintaining the culture under physiologically acceptable conditions and depleted nutrients are replenished. According to the present invention, human stem cells are efficiently obtained ex vivo by culturing human stem cells and/or human hematopoietic precursor cells and/or human stromal cells in a liquid culture medium which is continuously or periodically replaced, preferably perfused, and replenishing nutrients while maintaining the culture under physiologically acceptable conditions. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：为离体人干细胞增殖和稳定基因转化提供包括培养基条件，反应器等的新方法，以及包括培养基条件和反应器的新方法，用于（i）培养人造血前体细胞的最佳条件和（ii）人类造血前体细胞的稳定基因的转化。解决方案：公开了一种用于获得源自肝细胞系的离体人干细胞分裂产物的培养方法，其特征在于包含人造血系统的干细胞的细胞组合物在液体培养基中培养，所述液体培养基在每1mL培养物中1mL培养基的速率约24至约48小时，除去代谢产物，同时保持培养物在生理学上可接受的条件下并补充耗尽的营养物质。根据本发明，通过在连续或周期性更换，优选灌注的液体培养基中培养人类干细胞和/或人类造血前体细胞和/或人类基质细胞，从而有效获得人类干细胞，并补充营养物质同时保持生理学上可接受的条件下的培养。版权所有（C）2006，JPO＆NCIPI

2. 发明专利

JP2001120261A METHOD FOR CULTURING AND TRANSFORMING HUMAN STEM CELL- CONTAINING COMPOSITION 失效
公开(公告)号：JP2001120261A
公开(公告)日：2001-05-08
申请号：JP2000291494
申请日：2000-09-26
申请人： UNIV MICHIGAN
发明人： EMERSON STEPHEN G , CLARKE MICHAEL F , PALSSON BERNHARD O , SCHWARTZ RICHARD M
IPC分类号： A61K48/00 , C07K14/535 , C07K14/54 , C07K14/705 , C12M1/34 , C12M3/00 , C12M3/02 , C12M3/06 , C12N5/02 , C12N5/078 , C12N5/0787 , C12N5/0789 , C12N5/10 , C12N15/09 , C12N15/85 , C12N15/867 , C12N5/06
摘要： PROBLEM TO BE SOLVED: To obtain a human precursor cell proliferated product cultured ex vivo. SOLUTION: A human stem cell and/or a human hematopoietic precursor cell and/or a human interstitial cell are cultured in a liquid culture medium continuously or periodically exchanged or preferably perfused and nutrients are supplemented while holding the culturing under physiologically acceptable conditions. Thereby, the human precursor cell is efficiently obtained ex vivo in good yield.

3. 发明专利

JP2000189157A CULTURE OF HUMAN MAST CELL-CONTAINING COMPOSITION AND TRANSFORMATION 失效
公开(公告)号：JP2000189157A
公开(公告)日：2000-07-11
申请号：JP2000017257
申请日：2000-01-26
申请人： UNIV MICHIGAN
发明人： EMERSON STEPHEN G , CLARKE MICHAEL F , PALSSON BERNHARD O , SCHWARTZ RICHARD M
IPC分类号： A61K48/00 , C07K14/535 , C07K14/54 , C07K14/705 , C12M1/34 , C12M3/00 , C12M3/02 , C12M3/06 , C12N5/02 , C12N5/078 , C12N5/0787 , C12N5/0789 , C12N5/10 , C12N15/09 , C12N15/85 , C12N15/867
摘要： PROBLEM TO BE SOLVED: To obtain a propagated material of human hemopoietic system- originating precursor cells useful for the treatment of a cancer, etc., in ex vivo efficiently in a high yield by culturing a human hemopoietic system-originating precursor composition in a liquid culturing medium exchanged in a specific rate associated with the cell density in its culture. SOLUTION: This method for culturing a human mast cell-containing composition is to culture a human hemopoietic system-originating precursor composition such as human precursor cells, etc., found in human peripheral blood, human bone marrow, human fetal liver or human cord blood in a liquid culturing medium exchanged in a rate associated with the cell density of the culture i.e., in a rate corresponding to a rate for exchanging 500-100% per day so as to become 1×104-1×107 cells/ml medium cell density to obtain a human hemopoietic system-originating precursor cell propagated material in ex vivo. Further, it is preferable to use a medium containing IL-3, GM-CSF, steel factor, Epo, IL-1α, IL-1β, G-CSF, a basic fibroblast growth factor, IL-6 to IL-11, POGF or EFG.

4. 发明专利

JPH11221074A CULTURE METHOD FOR HUMAN STEM CELL-CONTAINING COMPOSITION AND TRANSFORMATION THEREOF 失效
公开(公告)号：JPH11221074A
公开(公告)日：1999-08-17
申请号：JP34291798
申请日：1998-12-02
申请人： UNIV MICHIGAN
发明人： EMERSON STEPHEN G , CLARKE MICHAEL F , PALSSON BERNHARD O , SCHWARTZ RICHARD M
IPC分类号： A61K48/00 , C07K14/535 , C07K14/54 , C07K14/705 , C12M1/34 , C12M3/00 , C12M3/02 , C12M3/06 , C12N5/02 , C12N5/078 , C12N5/0787 , C12N5/0789 , C12N5/10 , C12N15/09 , C12N15/85 , C12N15/867 , C12N5/06
摘要： PROBLEM TO BE SOLVED: To efficiently obtain a human dividing stem cell-containing composition that is useful for proliferation, transformation and the like of cultured mammalian cells by culturing human dividing stem cells observed in the human hematopoietic system ex vivo in the presence of hematopoietic stem cell grow factor. SOLUTION: Human dividing stem cells and/or human dividing hematopoietic precursor cells observed in bone marrow are continuously or periodically exchanged or perfused in a liquid culture medium, nutrients are supplied under the physiologically acceptable conditions and an effective amount of hematopoietic growth factors are added to culture these cells ex vivo thereby producing the objective human dividing stem cell composition that is useful for proliferation, transformation and the like of cultured mammalian cells. The hematopoietic cell growth factor to be used here is, for example, erythropoietin(Epo), interleukin-3(IL-3), granulocyte macrophage colony stimulation factor(GM-CSF), granulocyte colony stimulation factor(G-CSF), IL-6, IL-7, IL-11 or stem cell factor and the like.

5. 发明公开

EP0575350A4 METHOD FOR CULTURING AND TRANSFORMING HUMAN STEM CELL-CONTAINING COMPOSITIONS 失效
标题翻译：用于培养和转化含有人类细胞的组合物的方法
公开(公告)号：EP0575350A4
公开(公告)日：1997-07-02
申请号：EP92904136
申请日：1991-12-17
申请人： UNIV MICHIGAN
发明人： EMERSON STEPHEN G , CLARKE MICHAEL F , PALSSON BERNHARD O , SCHWARTZ RICHARD M
IPC分类号： A61K48/00 , C07K14/535 , C07K14/54 , C07K14/705 , C12M1/34 , C12M3/00 , C12M3/02 , C12M3/06 , C12N5/02 , C12N5/078 , C12N5/0787 , C12N5/0789 , C12N5/10 , C12N15/09 , C12N15/85 , C12N15/867 , C12M3/04 , C12N5/00 , C12N15/00
CPC分类号： C12N15/86 , A61K48/00 , C07K14/535 , C07K14/5403 , C07K14/5412 , C07K14/705 , C12M23/34 , C12M29/04 , C12M29/10 , C12M35/08 , C12N5/0641 , C12N5/0642 , C12N5/0647 , C12N15/85 , C12N2501/125 , C12N2501/14 , C12N2501/22 , C12N2501/23 , C12N2501/39 , C12N2502/13 , C12N2502/1394 , C12N2502/99 , C12N2740/13043 , C12N2830/002 , C12N2830/85
摘要： Methods, including culture media conditions and reactors, which provide for ex vivo human stem cell division and stable genetic transformation and/or the optimization of human hematopoietic progenitor cell cultures and/or increasing the metabolism or GM-CSF secretion or IL-6 secretion of human stromal cells are disclosed. The methods rely on culturing human stem cells and/or human hemapoietic progenitor cells and/or human stromal cells in a liquid culture medium which is replaced, preferably perfused, either continuously or periodically, at a rate of 1 ml of medium per ml of culture per about 24 to about 48 hour period, and removing metabolic products and replenishing depleted nutrients while maintaining the culture under physiologically acceptable conditions. Optionally growth factors are added to the culture medium.

6. 发明申请

WO9510619A3 METHODS OF INCREASING RATES OF INFECTION BY DIRECTING MOTION OF VECTORS 审中-公开
标题翻译：通过指导矢量运动来增加感染率的方法
公开(公告)号：WO9510619A3
公开(公告)日：1995-07-13
申请号：PCT/US9411285
申请日：1994-10-06
申请人： UNIV MICHIGAN
发明人： PALSSON BERNHARD O , CLARKE MICHAEL F , CHUCK ALICE S Y
IPC分类号： C12N15/09 , C12M3/00 , C12M3/06 , C12N15/87 , C12N15/63 , C12N15/67
CPC分类号： C12N15/87 , C12M25/04 , C12M35/02
摘要： This invention provides methods of increasing the frequency of contact between vectors and stationary target cells in an apparatus containing them which involves causing the vectors to move towards the target cells with motion above and beyond random Brownian motion. The methods of this invention include causing the vectors to move in the direction of the cells by (1) causing flow-through of a liquid containing the vectors through or past a cell bed, (2) moving charged vectors towards the target cells by electrodiffusion and (3) centrifuging vectors and cells to cause settling of vectors onto the cells.

7. 发明申请

WO9015877A3 METHODS, COMPOSITIONS AND DEVICES FOR GROWING CELLS 审中-公开
标题翻译：方法，用于生长细胞的组合物和装置
公开(公告)号：WO9015877A3
公开(公告)日：1991-01-24
申请号：PCT/US9003438
申请日：1990-06-14
申请人： UNIV MICHIGAN
发明人： EMERSON STEPHEN G , CLARKE MICHAEL F , PALSSON BERNHARD O
IPC分类号： C12M3/00 , A61K48/00 , C07K14/535 , C07K14/54 , C12M3/02 , C12M3/06 , C12N5/078 , C12N5/0787 , C12N5/0789 , C12N5/10 , C12P21/02 , C12P21/06 , C12R1/91 , C12M3/04 , C12N1/00 , C12N5/00 , C12N5/02 , C12N11/02 , C12N15/00
CPC分类号： C12N5/0647 , A61K48/00 , C07K14/535 , C07K14/5403 , C07K14/5412 , C12M25/02 , C12M29/10 , C12N5/0641 , C12N5/0642 , C12N2501/14 , C12N2501/22 , C12N2501/23 , C12N2502/13 , C12N2502/1323 , C12N2502/1394 , C12N2502/99
摘要： Methods, compositions and devices are provided for the growth of hematopoietic cells in culture. Bioreactors (10) are provided where appropriate levels of nutrients and growth factors are substantially continuously maintained in the bioreactor (10) while removing undesirable metabolic products. At least one growth factor is provided through excretion by transfected stromal cells, particularly heterologous cells. Elements (28, 30) are provided for maintaining the stromal cells and hematopoietic cells separately, to allow for easy removal of the hematopoietic cells.

8. 发明公开

EP0629236A4 METHODS, COMPOSITIONS AND DEVICES FOR MAINTAINING AND GROWING HUMAN STEM AND/OR HEMATOPOIETIC CELLS. 失效
标题翻译：方法，组成和支持维护和人类干细胞和/或细胞HÄMAPOETISCHER的生长。
公开(公告)号：EP0629236A4
公开(公告)日：1997-06-04
申请号：EP93908270
申请日：1993-03-04
申请人： UNIV MICHIGAN
发明人： PALSSON BERNHARD O , EMERSON STEPHEN G , SCHWARTZ RICHARD M
IPC分类号： C12M3/00 , C12M3/02 , C12M3/06
CPC分类号： C12M25/02 , C12M29/10 , C12M35/08
摘要： Methods, compositions and devices are provided for the growth of human stem and/or hematopoietic cells in culture. Bioreactors (106) are provided in which diverse cell types are simultaneously-cultured in the presence of appropriate levels of nutrients and growth factors substantially continuously maintained in the bioreactor (106) while removing undesirable metabolic products. This simultaneous culture of multiple cell types successfully reconstructs hematopoietic tissue ex vivo. Optionally, at least one growth factor is provided through excretion by transfected stromal cells, particularly heterologous cells. Means are provided for maintaining the stromal cells (112) and hematopoietic cells (110) separately, and to allow for harvesting of both the adherent (110, 112) and non-adherent cells (114).
摘要翻译：方法，组合物和装置提供了用于人类干细胞和/或培养物中造血细胞的生长。生物反应器（106）被提供在哪个各种细胞类型培养在存在同时-的营养物质和生长因子的适当水平基本上连续地保持在生物反应器（106），同时除去不需要的代谢产物。此多种细胞类型的培养的同时成功地重新构建造血组织离体进行。任选地，至少一种生长因子通过排泄由转染的细胞间质，特别是异源细胞中提供。被提供，用于分别保持所述基质细胞（112）和造血细胞（110），并允许这两个粘附（110，112）和非贴壁细胞（114）的收获装置。

9. 发明专利

DK0477290T3 未知
公开(公告)号：DK0477290T3
公开(公告)日：1997-07-21
申请号：DK90910240
申请日：1990-06-14
申请人： UNIV MICHIGAN
发明人： EMERSON STEPHEN G , CLARKE MICHAEL F , PALSSON BERNHARD O
IPC分类号： C12M3/00 , A61K48/00 , C07K14/535 , C07K14/54 , C12M3/02 , C12M3/06 , C12N5/078 , C12N5/0787 , C12N5/0789 , C12N5/10 , C12P21/02 , C12P21/06 , C12R1/91 , C12M3/04 , C12N1/00 , C12N5/02 , C12N11/02 , C12N15/00 , C12N5/00
摘要： A method and a composition of a culture medium are provided for obtaining ex vivo human progenitor cell expansion. The culture medium comprises human progenitor and/or stem cells, stromal cells and growth factors. The culture medium is replaced substantially continuously at a rate sufficient to maintain an effective growth environment for expansion of progenitor cells, and at least one portion of the stromal cells are transformed fibroblast cells capable of excreting at least one growth factor which directs the proliferation and/or differenciation of said human progenitor and/or stem cells.

10. 发明专利

CA2206600A1 METHODS, COMPOSITIONS AND APPARATUS FOR CELL TRANSFECTION 未知
公开(公告)号：CA2206600A1
公开(公告)日：1996-06-13
申请号：CA2206600
申请日：1995-12-06
申请人： UNIV MICHIGAN
发明人： PALSSON BERNHARD O
IPC分类号： C12N15/09 , A61K48/00 , C12M1/00 , C12M3/06 , C12N5/10 , C12N7/00 , C12N11/00 , C12N15/87 , C12M1/12 , C12N15/86
摘要： This invention provides methods, compositions and apparatus for increasing the transfection efficiency of target cells by particles, especially retroviral particles, compared with that achieved by current methods. The transfection method comprises depositing the particles on a cell growth support and contacting target cells with the particle-loaded cell growth support.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式