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1. 发明申请

WO2008048231A3 BIOSENSOR METHOD AND SYSTEM BASED ON FEATURE VECTOR EXTRACTION 审中-公开
标题翻译：基于特征向量提取的生物传感器方法和系统
公开(公告)号：WO2008048231A3
公开(公告)日：2008-07-24
申请号：PCT/US2006032772
申请日：2006-08-18
申请人： UT BATTELLE LLC , GREENBAUM ELIAS , RODRIGUEZ MIGUEL JR , QI HAIRONG , WANG XIAOLING
发明人： GREENBAUM ELIAS , RODRIGUEZ MIGUEL JR , QI HAIRONG , WANG XIAOLING
IPC分类号： G06K9/00 , C12Q1/02
CPC分类号： C12Q1/025
摘要： A method of biosensor-based detection of toxins includes the steps of providing at least one time-dependent control signal generated by a biosensor in a gas or liquid medium, and obtaining a time-dependent biosensor signal from the biosensor in the gas or liquid medium to be monitored or analyzed for the presence of one or more toxins selected from chemical, biological or radiological agents. The time-dependent biosensor signal is processed to obtain a plurality of feature vectors using at least one of amplitude statistics and a time- frequency analysis. At least one parameter relating to toxicity of the gas or liquid medium is then determined from the feature vectors based on reference to the control signal. A water or air quality sensor system 10 includes photosynthetic organisms as a biosensor in an air or water medium to be monitored or analyzed for the presence of one or more toxins, a detector 12 proximate to the biosensor for measuring a time-dependent biosensor signal from said biosensor, and a processor 30 for analyzing the time-dependent biosensor signal to obtain a plurality of feature vectors using amplitude statistics or time-frequency analysis, wherein the processor determines at least one parameter relating to toxicity of the medium from the feature vectors.
摘要翻译：一种基于生物传感器的毒素检测方法包括以下步骤：在气体或液体介质中提供由生物传感器产生的至少一个时间依赖的控制信号，并从气体或液体介质中的生物传感器获得与时间相关的生物传感器信号被监测或分析一种或多种选自化学，生物或放射剂的毒素的存在。处理时间依赖的生物传感器信号以使用幅度统计和时间 - 频率分析中的至少一个来获得多个特征向量。然后，基于对控制信号的参考，从特征向量确定与气体或液体介质的毒性有关的至少一个参数。水或空气质量传感器系统10包括光合生物作为待监测或分析的空气或水介质中的生物传感器，用于存在一种或多种毒素;邻近生物传感器的检测器12，用于测量时间依赖性生物传感器信号所述生物传感器，以及处理器30，其用于使用幅度统计或时间 - 频率分析来分析所述时间依赖性生物传感器信号以获得多个特征向量，其中所述处理器从所述特征向量确定与所述介质的毒性有关的至少一个参数。

2. 发明申请

WO2008054394A9 METHOD AND APPARATUS FOR DETECTION OF TOXIC AGENTS USING PHOTOSYNTHETIC ORGANISMS 审中-公开
标题翻译：使用光合作用有机物检测毒性试剂的方法和装置
公开(公告)号：WO2008054394A9
公开(公告)日：2008-07-31
申请号：PCT/US2006042984
申请日：2006-11-03
申请人： UT BATTELLE LLC , GREENBAUM ELIAS , RODRIGUEZ MIGUEL JR , WU JIE JAYNE , QI HAIRONG
发明人： GREENBAUM ELIAS , RODRIGUEZ MIGUEL JR , WU JIE JAYNE , QI HAIRONG
IPC分类号： C12Q1/02 , G01N33/53
CPC分类号： C12Q1/025
摘要： A water quality analyzer for real-time detection according to the invention (100) comprises a biased AC electro-osmosis (ACEO) cell (154) for receiving a fluid to be analyzed having a plurality photosynthetic organisms therein, and concentrating the plurality photosynthetic organisms into at least one concentrated region. A photodetector (157) is provided for obtaining a measured photosynthetic activity of the plurality of photosynthetic organisms in the concentrated region responses to incident light from light source (105), wherein chemical, biological or radiological agents reduce a nominal photosynthetic activity of the photosynthetic organisms. An electronics package (158) analyzes the measured photosynthetic activity to indicate a presence of the chemical, biological or radiological agents in the fluid.
摘要翻译：根据本发明（100）的用于实时检测的水质分析器包括偏置AC电渗透（ACEO）池（154），用于接收待分析的流体，其中具有多个光合生物，并且浓缩多个光合生物进入至少一个集中区域。提供了一种光电检测器（157），用于获得在浓缩区域对来自光源（105）的入射光的响应中多个光合生物测量的光合活性，其中化学，生物或放射性试剂降低光合生物的标称光合活性。电子封装（158）分析测量的光合活性以指示在流体中存在化学，生物或放射性试剂。

3. 发明申请

WO2008054394A2 METHOD AND APPARATUS FOR ENHANCED DETECTION OF TOXIC AGENTS 审中-公开
标题翻译：增强毒性试剂检测方法和装置
公开(公告)号：WO2008054394A2
公开(公告)日：2008-05-08
申请号：PCT/US2006042984
申请日：2006-11-03
申请人： UT BATTELLE LLC , GREENBAUM ELIAS , RODRIGUEZ MIGUEL JR , WU JIE JAYNE , QI HAIRONG
发明人： GREENBAUM ELIAS , RODRIGUEZ MIGUEL JR , WU JIE JAYNE , QI HAIRONG
IPC分类号： C12Q1/02 , G01N33/53
CPC分类号： C12Q1/025
摘要： A water quality analyzer for real-time detection according to the invention (100) comprises a biased AC electro-osmosis (ACEO) cell (154) for receiving a fluid to be analyzed having a plurality photosynthetic organisms therein, and concentrating the plurality photosynthetic organisms into at least one concentrated region. A photodetector (157) is provided for obtaining a measured photosynthetic activity of the plurality of photosynthetic organisms in the concentrated region responses to incident light from light source (105), wherein chemical, biological or radiological agents reduce a nominal photosynthetic activity of the photosynthetic organisms. An electronics package (158) analyzes the measured photosynthetic activity to indicate a presence of the chemical, biological or radiological agents in the fluid.
摘要翻译：根据本发明的用于实时检测的水质分析仪（100）包括偏置AC电渗透（ACEO）池（154），用于接收待分析的流体，其中具有多个光合生物，并且浓缩多个光合生物进入至少一个集中区域。提供了一种光电检测器（157），用于获得多个光合生物在浓缩区域对来自光源（105）的入射光的响应的测量的光合活性，其中化学，生物或放射性试剂降低光合生物的标称光合活性。电子封装（158）分析测量的光合活性以指示流体中化学，生物或放射性试剂的存在。

4. 发明专利

DE602006007734D1 未知
公开(公告)号：DE602006007734D1
公开(公告)日：2009-08-20
申请号：DE602006007734
申请日：2006-12-13
申请人： UT BATTELLE LLC
发明人： GREENBAUM ELIAS , RODRIGUEZ MIGUEL JR
IPC分类号： C12Q1/02 , C12Q1/22
摘要： A method of biosensor-based detection of toxins includes the steps of providing a fluid to be analyzed having a plurality of photosynthetic organisms therein, wherein chemical, biological or radiological agents alter a nominal photosynthetic activity of the photosynthetic organisms. At a first time a measured photosynthetic activity curve is obtained from the photosynthetic organisms. The measured curve is automatically compared to a reference photosynthetic activity curve to determine differences therebetween. The presence of the chemical, biological or radiological agents, or precursors thereof, are then identified if present in the fluid using the differences.

5. 发明专利

CA2629307C METHOD AND APPARATUS FOR ENHANCED DETECTION OF TOXIC AGENTS 未知
公开(公告)号：CA2629307C
公开(公告)日：2014-05-27
申请号：CA2629307
申请日：2006-11-03
申请人： UT BATTELLE LLC , UNIV TENNESSEE RES FOUNDATION
发明人： GREENBAUM ELIAS , RODRIGUEZ MIGUEL JR , WU JIE JAYNE , QI HAIRONG
IPC分类号： C12Q1/02 , G01N33/53
摘要： A water quality analyzer for real-time detection according to the invention (100) comprises a biased AC electro-osmosis (ACEO) cell (154) for receiving a fluid to be analyzed having a plurality photosynthetic organisms therein, and concentrating the plurality photosynthetic organisms into at least one concentrated region. A photodetector (157) is provided for obtaining a measured photosynthetic activity of the plurality of photosynthetic organisms in the concentrated region responses to incident light from light source (105), wherein chemical, biological or radiological agents reduce a nominal photosynthetic activity of the photosynthetic organisms. An electronics package (158) analyzes the measured photosynthetic activity to indicate a presence of the chemical, biological or radiological agents in the fluid.

6. 发明专利

CA2633983A1 METHOD AND SYSTEM FOR REAL-TIME ANALYSIS OF BIOSENSOR DATA 未知
公开(公告)号：CA2633983A1
公开(公告)日：2007-06-21
申请号：CA2633983
申请日：2006-12-13
申请人： UT BATTELLE LLC
发明人： RODRIGUEZ MIGUEL JR , GREENBAUM ELIAS
IPC分类号： C12Q1/02 , C12Q1/22
摘要： A method of biosensor-based detection of toxins includes the steps of providing a fluid to be analyzed having a plurality of photosynthetic organisms therein, wherein chemical, biological or radiological agents alter a nominal photosynthetic activity of the photosynthetic organisms. At a first time a measured photosynthetic activity curve is obtained from the photosynthetic organisms. The measured curve is automatically compared to a reference photosynthetic activity curve to determine differences therebetween. The presence of the chemical, biological or radiological agents, or precursors thereof, are then identified if present in the fluid using the differences.

7. 发明专利

DE602006008979D1 未知
公开(公告)号：DE602006008979D1
公开(公告)日：2009-10-15
申请号：DE602006008979
申请日：2006-11-03
申请人： UT BATTELLE LLC
发明人： GREENBAUM ELIAS , RODRIGUEZ MIGUEL JR , WU JIE JAYNE , QI HAIRONG
IPC分类号： C12Q1/02 , G01N33/53
摘要： A biosensor based detection of toxins includes enhancing a fluorescence signal by concentrating a plurality of photosynthetic organisms in a fluid into a concentrated region using biased AC electro-osmosis. A measured photosynthetic activity of the photosynthetic organisms is obtained in the concentrated region, where chemical, biological or radiological agents reduce a nominal photosynthetic activity of the photosynthetic organisms. A presence of the chemical, biological and/or radiological agents or precursors thereof, is determined in the fluid based on the measured photosynthetic activity of the concentrated plurality of photosynthetic organisms. A lab-on-a-chip system is used for the concentrating step. The presence of agents is determined from feature vectors, obtained from processing a time dependent signal using amplitude statistics and/or time-frequency analysis, relative to a control signal. A linear discriminant method including support vector machine classification (SVM) is used to identify the agents.

8. 发明专利

CA2629307A1 METHOD AND APPARATUS FOR ENHANCED DETECTION OF TOXIC AGENTS 未知
公开(公告)号：CA2629307A1
公开(公告)日：2008-05-08
申请号：CA2629307
申请日：2006-11-03
申请人： UT BATTELLE LLC
发明人： RODRIGUEZ MIGUEL JR , GREENBAUM ELIAS , WU JIE JAYNE , QI HAIRONG
IPC分类号： C12Q1/02 , G01N33/53
摘要： A water quality analyzer for real-time detection according to the invention (100) comprises a biased AC electro-osmosis (ACEO) cell (154) for receiving a fluid to be analyzed having a plurality photosynthetic organisms therein, and concentrating the plurality photosynthetic organisms into at least one concentrated region. A photodetector (157) is provided for obtaining a measured photosynthetic activity of the plurality of photosynthetic organisms in the concentrated region responses to incident light from light source (105), wherein chemical, biological or radiological agents reduce a nominal photosynthetic activity of the photosynthetic organisms. An electronics package (158) analyzes the measured photosynthetic activity to indicate a presence of the chemical, biological or radiological agents in the fluid.

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