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1. 发明申请

WO2007095871A3 SUPERPARAMAGNETIC NANOPARTICLES BASED ON IRON OXIDES WITH MODIFIED SURFACE, METHOD OF THEIR PREPARATION AND APPLICATION 审中-公开
公开(公告)号：WO2007095871A3
公开(公告)日：2007-08-30
申请号：PCT/CZ2007/000012
申请日：2007-02-23
申请人： USTAV MAKROMOLEKULARNI CHEMIE AKADEMIE VEDCESKE REPUBLIKY, V.V.I , USTAV EXPERIMENTALNI MEDICINY AKADEMIE VEDCESKE REPUBLIKY.V.V.I , HORAK, Daniel , SYKOVA, Eva , BABIC, Michal , JENDELOVA, Pavla , HAJEK, Milan
发明人： HORAK, Daniel , SYKOVA, Eva , BABIC, Michal , JENDELOVA, Pavla , HAJEK, Milan
IPC分类号： C01G49/08 , C01G49/06 , A61K49/18 , C09C1/24
摘要： The subject of the invention is superparamagnetic nanoparticle probes based on iron oxides, to advantage magnetite or maghemite, with modified surface, coated with mono-, di- or polysaccharides from the group including D-arabinose, D-glucose, D-galactose, D-mannose, lactose, maltose, dextrans and dextrins, or with amino acids or poly(amino acid)s from the group including alanine, glycine, glutamine, asparagine, histidine, arginine, L-lysine, aspartic and glutamic acid or with synthetic polymers based on (meth)acrylic acid and their derivatives selected from the group containing poly( N , N -dimethylacrylamide), poly( N,N - dimethylmethacrylamide), poly( N, N- diethylacrylamide), poly (N, iV-diethylmethacrylamide), poly( N -isopropylacrylamide), poly( N -isopropylmethacrylamide), which form a colloid consisting of particles with narrow distribution with polydispersity index smaller than 1.3, the average size of which amounts to 0.5-30 nm, to advantage 1-10 nm, the iron content is 70- 99.9 wt.%, to advantage 90 wt.%, the modification agent content 0.1-30 wt.%, to advantage 10 wt.%. The particles of size smaller than 2 nm with polydispersity index smaller than 1.1 can be obtained by a modified method of preparation. Superparamagnetic nanoparticle probes according to the invention are prepared by pre- precipitation of colloidal Fe(OH) 3 by the treatment of aqueous 0.1-0.2M solution of Fe(III) salt, to advantage FeCl 3 , with less than an equimolar amount Of NH 4 OH, at 21°C, under sonication, to which a solution of a Fe(II) salt, to advantage FeCl2, is added in the mole ratio Fe(III)/Fe(II) = 2 under sonication and the mixture is poured into five- to tenfold, to advantage eightfold, molar excess of 0.5M NH 4 OH. The mixture is left aging for 0-30 min, to advantage 15 min, and then the precipitate is repeatedly, to advantage 7-10 times, magnetically separated and washed with deionized water. Then 1-3 fold amount, to advantage 1.5 fold amount, relative to the amount of magnetite, of 0.1 M aqueous solution of sodium citrate is added and then, drop wise, 1-3 fold amount, to advantage 1.5 fold amount, relative to the amount of magnetite, of 0.7 M aqueous solution of sodium hypochlorite. The precipitate is repeatedly, to advantage 7-10 times, washed with deionized water under the formation of colloidal maghemite to which, after dilution, is added drop wise, to advantage under 5 -min sonication, an aqueous solution of a modification agent, in the weight ratio modification agent/iron oxide = 0.1-10, to advantage 0.2 for amino acids and poly(amino acid)s and 5 for saccharides. The particles smaller than 2 nm. with polydispersity index smaller than 1.1 are prepared by mixing at 21°C 1 volume part of 10-60 wt. %, to advantage 50 wt.%, of an aqueous solution of a saccharide, disaccharide or polysaccharide, such as D-arabinose, D-glucose, D-galactose, D-mannose, lactose, maltose, dextran and dextrins, and 1 volume part of aqueous solution of a Fe(II) and Fe(III) salt, to advantage FeCl 2 and FeCl 3 , where the molar ratio Fe(III)/Fe(II) = 2. A 5-15 %, to advantage 7.5 %, solution Of NH 4 OH is added until pH 12 is attained and

2. 发明申请

WO2011098053A1 USE OF GALECTINS AND METHOD OF PREPARATION OF MYOFIBROBLASTS AND/OR EXTRACELLULAR MATRIX NANOFIBERS 审中-公开
标题翻译：糖蛋白和/或细胞外基质纳米纤维的制备及其制备方法的应用
公开(公告)号：WO2011098053A1
公开(公告)日：2011-08-18
申请号：PCT/CZ2011/000014
申请日：2011-02-07
申请人： UNIVERZITA KARLOVA V PRAZE , USTAV EXPERIMENTALNI MEDICINY AV CR, v. v. i. , LUDWIG-MAXIMILIANS UNIVERSITÄT , SMETANA, Karel , GABIUS, Hans-Joachim , DVORANKOVA, Barbora , KALTNER, Herbert , LACINA, Lukas , ANDRE, Sabine , SZABO, Pavol , VALACH, Jaroslav , ZIMA, Tomas , SYKOVA, Eva , JENDELOVA, Pavla
发明人： SMETANA, Karel , GABIUS, Hans-Joachim , DVORANKOVA, Barbora , KALTNER, Herbert , LACINA, Lukas , ANDRE, Sabine , SZABO, Pavol , VALACH, Jaroslav , ZIMA, Tomas , SYKOVA, Eva , JENDELOVA, Pavla
IPC分类号： C12N5/00
CPC分类号： C12N5/0068 , C12N5/0658 , C12N2501/15 , C12N2501/59 , C12N2506/1307 , C12N2533/52
摘要： The invention relates to the use of at least one recombinant mammalian galectin selected from the group comprising galectin-1, galectin-4 and galectin-7 as inductors of fibroblast-to-myofibroblast transition and/or production of extracellular matrix nanofibers. The invention further relates to a method of preparation of myofibroblasts and/or extracellular matrix nanofibers wherein the fibroblasts are in vitro cultivated in the presence of at least one recombinant mammalian galectin selected from the group comprising galectin-1, galectin-4 and galectin-7. Combination of a galectin and TGF-ß1 exerts a synergic effect. Further, the invention relates to extracellular matrix nanofibers in the form of three-dimensional scaffolds obtainable by the method of this invention. ˙
摘要翻译：本发明涉及至少一种选自Galectin-1，galectin-4和galectin-7的重组哺乳动物半乳凝素作为成纤维细胞与肌成纤维细胞转化和/或细胞外基质纳米纤维的生产的电感器的用途。本发明还涉及一种制备肌成纤维细胞和/或细胞外基质纳米纤维的方法，其中成纤维细胞在至少一种选自Galectin-1，半乳凝素-4和半乳凝素-7的重组哺乳动物半乳凝素存在下体外培养。 Galectin和TGF-β1的组合发挥协同作用。此外，本发明涉及通过本发明的方法可获得的三维支架形式的细胞外基质纳米纤维。？

3. 发明申请

WO2008089708A1 BIOMATERIAL BASED ON NANOFIBRILLAR LAYERS AND METHOD OF PREPARATION THEREOF 审中-公开
标题翻译：基于NANOFIBRILLAR层的生物材料及其制备方法
公开(公告)号：WO2008089708A1
公开(公告)日：2008-07-31
申请号：PCT/CZ2008/000005
申请日：2008-01-09
申请人： USTAV EXPERIMENTALNI MEDICINY AV CR, v.v.i. , USTAV MAKROMOLEKULARNI CHEMIE AV CR, v.v.i. , TECHNICKA UNIVERZITA V LIBERCI , LESNY, Petr , SYKOVA, Eva , MICHALEK, Jiri , PRADNY, Martin , JIRSAK, Oldrich , MARTINOVA, Lenka
发明人： LESNY, Petr , SYKOVA, Eva , MICHALEK, Jiri , PRADNY, Martin , JIRSAK, Oldrich , MARTINOVA, Lenka
IPC分类号： A61L27/38 , A61L27/56 , A61L27/16 , A61L27/18
CPC分类号： A61L27/50 , A61L27/14 , A61L27/38 , A61L2400/12 , C12N5/0068 , C12N2533/30 , D01D5/0007
摘要： The invention relates to a biomaterial based on nanofibrillar layers, which consists of at least one nanofibrillar layer and living cells firmly connected with this nanofibrillar layer, wherein the nanofibrillar layer is formed by synthetic polymers or co-polymers of monomers selected from the group comprising methacrylic acid esters, methacrylic acid amides, poly urethanes, polyvinyl alcohols and polymers derived from lactic acid and its derivatives, and to a method of preparation thereof.
摘要翻译：本发明涉及一种基于纳米纤丝层的生物材料，其由至少一个纳米纤丝层和与该纳米纤丝层牢固连接的活细胞组成，其中纳米纤丝层由合成聚合物或选自以下的单体的共聚物形成：甲基丙烯酸酸酯，甲基丙烯酸酰胺，聚氨酯，聚乙烯醇和衍生自乳酸及其衍生物的聚合物及其制备方法。

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