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1. 发明申请

WO1998015627A1 PRODUCTION OF RECOMBINANT PEPTIDE BY TRANSGENIC FISH 审中-公开
标题翻译：通过转基因鱼生产重组蛋白
公开(公告)号：WO1998015627A1
公开(公告)日：1998-04-16
申请号：PCT/GB1997002806
申请日：1997-10-10
申请人： UNIVERSITY OF SOUTHAMPTON , ROYAL FREE HOSPITAL SCHOOL OF MEDICINE , MACLEAN, Norman , WILLIAMS, Darren, William , GOLDSPINK, Geoffrey , MÜLLER, Ferenc
发明人： UNIVERSITY OF SOUTHAMPTON , ROYAL FREE HOSPITAL SCHOOL OF MEDICINE
IPC分类号： C12N15/12
CPC分类号： A01K67/0275 , A01K2217/05 , C07K14/745
摘要： The invention provides an expression construct for expressing a polypeptide in fish, which construct comprises a nucleic acid coding sequence encoding the polypeptide operably linked to a regulatory sequence capable of directing the expression of the coding sequence in a cell of the fish. It also provides methods of using such constructs to produce recombinant polypeptides in transgenic fish.
摘要翻译：本发明提供了用于在鱼中表达多肽的表达构建体，该构建体包含编码多肽的核酸编码序列，其可操作地连接于能够引导编码序列在鱼细胞中的表达的调节序列。它还提供了使用这种构建体在转基因鱼中产生重组多肽的方法。

2. 发明申请

WO1994028151A1 GENE THERAPY FOR HAEMOPHILIA 审中-公开
标题翻译：天疱疮基因治疗
公开(公告)号：WO1994028151A1
公开(公告)日：1994-12-08
申请号：PCT/GB1994001114
申请日：1994-05-20
申请人： ROYAL FREE HOSPITAL SCHOOL OF MEDICINE , MEDICAL RESEARCH COUNCIL , GOLDSPINK, Geoffrey , LEE, Christine , TUDDENHAM, Edward
发明人： ROYAL FREE HOSPITAL SCHOOL OF MEDICINE , MEDICAL RESEARCH COUNCIL
IPC分类号： C12N15/85
CPC分类号： C12N15/85 , A61K38/00 , C07K14/755 , C07K2319/02 , C12N2830/008 , C12N2830/30 , C12N2830/85
摘要： The invention provides a recombinant nucleic acid construct comprising: (i) a mammalian myosin heavy chain promoter; (ii) nucleic acid encoding a signal sequence; and (iii) nucleic acid encoding a functional blood clotting protein; the elements (i)-(iii) being operably linked to provide for expression of the functional protein. The construct is useful for the treatment of diseases such as haemophilia by introducing it into muscle cells. The activity of the promoter can be enhanced by exercise or stimulation of the muscle cells.
摘要翻译：本发明提供重组核酸构建体，其包含：（i）哺乳动物肌球蛋白重链启动子; （ii）编码信号序列的核酸; 和（iii）编码功能性凝血蛋白的核酸; 元件（i） - （iii）可操作地连接以提供功能性蛋白质的表达。该构建体可用于通过将其引入肌细胞来治疗诸如血友病的疾病。通过肌肉细胞的运动或刺激可以增强启动子的活性。

3. 发明申请

WO1997033997A1 METHOD OF TREATING MUSCULAR DISORDERS 审中-公开
标题翻译：治疗音乐障碍的方法
公开(公告)号：WO1997033997A1
公开(公告)日：1997-09-18
申请号：PCT/GB1997000658
申请日：1997-03-11
申请人： ROYAL FREE HOSPITAL SCHOOL OF MEDICINE , GOLDSPINK, Geoffrey
发明人： ROYAL FREE HOSPITAL SCHOOL OF MEDICINE
IPC分类号： C12N15/16
CPC分类号： C07K14/65 , A61K38/00
摘要： An IGF-I polypeptide or functional derivative thereof characterized by the presence of the Ec peptide or functional equivalent thereof for use in a method of treatment or therapy of the human or animal body.
摘要翻译： IGF-1多肽或其功能性衍生物，其特征在于存在Ec肽或其功能等同物，用于治疗或治疗人或动物体的方法。

4. 发明申请

WO1993002215A1 QUANTITATIVE NUCLEIC ACID AMPLIFICATION 审中-公开
标题翻译：定量核酸放大
公开(公告)号：WO1993002215A1
公开(公告)日：1993-02-04
申请号：PCT/GB1992001355
申请日：1992-07-23
申请人： ROYAL FREE HOSPITAL SCHOOL OF MEDICINE , GRIFFITHS, Paul , EMERY, Vincent , FOX, Jayne
发明人： ROYAL FREE HOSPITAL SCHOOL OF MEDICINE
IPC分类号： C12Q01/68
CPC分类号： C12Q1/703 , C12Q1/6851 , C12Q1/705 , C12Q2545/107 , C12Q2525/131
摘要： The invention provides a method to determine the amount of a target nucleic acid in a sample which comprises (i) mixing the sample with a predetermined amount of a control nucleic acid, the control nucleic acid having a control region which includes at least one primer binding region homologous to a primer binding region in a selected region of the target nucleic acid; (ii) bringing the mixture formed in (i) into contact with at least one nucleic acid primer capable of binding to the primer binding region of the target and control nucleic acids; (iii) performing a nucleic acid amplification reaction, said reaction requiring the presence of the primer to amplify the selected region of the target nucleic acid and the control region of the control nucleic acid; (iv) determining the relative quantities of the amplified control region and selected region nucleic acids; and (v) calculating from the determination of (iv) the amount of target nucleic acid in the sample. The method can be used for the quantitation of a PCR, e.g. using a control nucleic acid which has a centrally located unique restriction site not present in the target nucleic acid. Target nucleic acids may include viral genomes of e.g. CMV.
摘要翻译：本发明提供了一种确定样品中靶核酸量的方法，其包括（i）将样品与预定量的对照核酸混合，所述对照核酸具有包含至少一种引物结合的对照区区域与靶核酸的选定区域中的引物结合区同源; （ii）使（i）中形成的混合物与能够结合靶和靶核酸的引物结合区的至少一个核酸引物接触; （iii）进行核酸扩增反应，所述反应需要引物的存在以扩增靶核酸的选定区域和对照核酸的对照区域; （iv）确定扩增对照区和选择区核酸的相对量; 和（v）从（iv）样品中靶核酸的量的确定计算。该方法可用于定量PCR，例如，使用具有在靶核酸中不存在的位于中心的独特的限制性位点的对照核酸。靶核酸可以包括例如病毒基因组。 CMV。

5. 发明申请

WO1990004606A1 A PROCESS FOR FRACTIONATING POLYETHYLENE GLYCOL (PEG)-PROTEIN ADDUCTS AND AN ADDUCT OF PEG AND GRANULOCYTE-MACROPHAGE COLONY STIMULATING FACTOR 审中-公开
标题翻译：用于分离聚乙二醇（PEG） - 葡聚糖添加剂的方法和PEG和颗粒聚合物 - 胶束聚集刺激因子的添加
公开(公告)号：WO1990004606A1
公开(公告)日：1990-05-03
申请号：PCT/GB1989001261
申请日：1989-10-20
申请人： ROYAL FREE HOSPITAL SCHOOL OF MEDICINE , FISHER, Derek , FRANCIS, Gillian, Elizabeth , DELGADO, Cristina
发明人： ROYAL FREE HOSPITAL SCHOOL OF MEDICINE
IPC分类号： C07K17/08
CPC分类号： A61K47/60 , G01N30/42
摘要： A process for fractionating a mixture of polyethylene glycol (PEG)-protein adducts comprising partitioning the PEG/protein adducts in a PEG-containing aqueous biphasic system.
摘要翻译：分离聚乙二醇（PEG） - 蛋白质加合物的混合物的方法，包括将PEG /蛋白质加合物分配在含PEG的水性双相体系中。

6. 发明申请

WO1997003345A2 DETECTION AND QUANTIFICATION OF HUMAN HERPESVIRUS 7 BY ENZYMIC AMPLIFICATION 审中-公开
标题翻译：通过酶扩增检测和定量人类草药7
公开(公告)号：WO1997003345A2
公开(公告)日：1997-01-30
申请号：PCT/GB1996001660
申请日：1996-07-12
申请人： ROYAL FREE HOSPITAL SCHOOL OF MEDICINE , EMERY, Vincent, Clive , GRIFFITHS, Paul
发明人： ROYAL FREE HOSPITAL SCHOOL OF MEDICINE
IPC分类号： G01N00/00
CPC分类号： C12Q1/705 , C12Q1/6851
摘要： An isolated nucleic acid molecule comprising: (i) a primer portion consisting of a contiguous sequence of from 10 to 50 nucleotides capable of hybridising to (a) the target nucleic acid molecule represented by SEQ. ID No. 1, or (b) to the complementary stand thereof; and optionally (ii) a further portion comprising from 1 to 25 nucleotides joined to and immediately 5' to the 5' end of the primer portion.
摘要翻译：一种分离的核酸分子，其包含：（i）引物部分，其由能够与（a）SEQ ID NO：1所代表的靶核酸分子杂交的10至50个核苷酸的连续序列组成。 ID号1，或（b）其互补立场; 和任选地（ii）包含1至25个核苷酸的另外部分，其连接到引物部分的5'末端并立即连接到5'末端。

7. 发明申请

WO1995006058A1 POLYMER MODIFICATION 审中-公开
标题翻译：聚合物改性
公开(公告)号：WO1995006058A1
公开(公告)日：1995-03-02
申请号：PCT/GB1994001844
申请日：1994-08-23
申请人： ROYAL FREE HOSPITAL SCHOOL OF MEDICINE , FRANCIS, Gillian, Elizabeth , FISHER, Derek , DELGADO, Cristina , MALIK, Farooq
发明人： ROYAL FREE HOSPITAL SCHOOL OF MEDICINE
IPC分类号： C07K01/10
CPC分类号： C07K1/1077 , A61K47/60
摘要： A process for producing adducts of a polymer and a target material which process comprises the steps of (a) reacting either (i) an activating compound of formula (I) X-AM with a polymer of formula (II) (C)c POL - Gg so as to form (ii) a sulphonate ester-activated polymer of formula (III) (C)c POL - (AM)g; (b) reacting the sulphonate ester-activated polymer of formula (III) or (III') with the target material and (c) recovering the adduct of the polymer and the target material, in which process: (i) the polymer of formula (II) is dry as determined by benzene distillation, (ii) the reaction of the compound of formula (I) or (I') with the polymer of formula (II) is conducted in an organic solvent which is inert to the reagents and to the product of formula (III) or (III') and is anhydrous as obtainable using molecular sieves of 0.3nm; (iii) the reaction of the compound of formula (I) or (I') with the polymer of formula (II) is conducted in a reaction vessel from which water is excluded; (iv) the sulphonate ester-activated polymer of formula (III) or (III') so produced is recovered and either used directly in step (b) or stored, prior to use in step (b), in the presence of a desiccating agent more hygroscopic than the product of formula (III) or (III'); and (v) the reaction of the sulphonate ester-activated polymer with the target material is conducted in a non-denaturing medium and non-denaturing temperature with respect to the target material.
摘要翻译：一种制备聚合物和目标材料的加合物的方法，该方法包括以下步骤：（i）使式（I）X-AM的活化化合物与式（II）（C）的聚合物反应 -Gg，以形成（ⅱ）式（Ⅳ）的磺酸酯活化聚合物（C）c POL - （AM）g; （b）使式（III）或（III'）的磺酸酯活化的聚合物与目标材料反应，和（c）回收聚合物和目标材料的加合物，其中：（i）式（II）通过苯蒸馏测定为干燥，（ii）式（I）或（I'）化合物与式（II）的聚合物的反应在对试剂呈惰性的有机溶剂中进行，（III）或（III'）的产物，并且使用0.3nm的分子筛可得到无水的; （iii）式（I）或（I'）化合物与式（II）的聚合物的反应在排除水的反应容器中进行; （iv）如此生产的式（III）或（III'）的磺酸酯活化的聚合物被回收，并且在步骤（b）中直接使用或在步骤（b）中使用之前）在干燥剂试剂比式（III）或（III'）的产品更吸湿; 和（v）磺酸酯活化的聚合物与目标材料的反应在相对于目标材料的非变性介质和非变性温度下进行。

8. 发明申请

WO1994021815A1 PREDICTIVE ASSAY FOR THE OUTCOME OF IVF 审中-公开
标题翻译： IVF成果的预测性测定
公开(公告)号：WO1994021815A1
公开(公告)日：1994-09-29
申请号：PCT/GB1994000596
申请日：1994-03-23
申请人： ROYAL FREE HOSPITAL SCHOOL OF MEDICINE , COOKE, Brian , MICHAEL, Anthony
发明人： ROYAL FREE HOSPITAL SCHOOL OF MEDICINE
IPC分类号： C12Q01/32
CPC分类号： C12Q1/32 , G01N33/573 , Y10S435/806 , Y10S436/817
摘要： The present invention provides a method for predicting the outcome of IVF which comprises: (i) determining the level of 11 beta -hydroxysteroid dehydrogenase (11 beta -HSD) in a biological sample from a female patient; and (ii) predicting from the level of 11 beta -HSD determined the probability of establishing pregnancy in said subject by IVF. It has been found that there is an inverse correlation between the levels of 11 beta -HSD in the environment of the oocyte and subsequent establishment of pregnancy by IVF. The present invention also provides methods of screening female subjects for their suitability to take part in IVF programs, and kits to use in determining levels of 11 beta -HSD.
摘要翻译：本发明提供了一种用于预测IVF结果的方法，其包括：（i）测定来自女性患者的生物样品中11β-羟基类固醇脱氢酶（11β-HSD）的水平; 和（ii）从11个beta-HSD的水平预测，确定了IVF在所述受试者中建立怀孕的概率。已经发现，在卵母细胞环境中的11β-HSD水平与随后的IVF建立怀孕之间存在负相关。本发明还提供筛选女性受试者适合参与IVF方案的方法，以及用于确定11β-HSD水平的试剂盒。

9. 发明申请

WO1990004650A1 A PURIFICATION PROCESS FOR COVALENTLY BOUND DNA/PROTEIN COMPLEXES 审中-公开
标题翻译：共价DNA /蛋白复合物的纯化方法
公开(公告)号：WO1990004650A1
公开(公告)日：1990-05-03
申请号：PCT/GB1989001263
申请日：1989-10-20
申请人： ROYAL FREE HOSPITAL SCHOOL OF MEDICINE , FISHER, Derek , FRANCIS, Gillian, Elizabeth , ANDERSON, Robert
发明人： ROYAL FREE HOSPITAL SCHOOL OF MEDICINE
IPC分类号： C12Q01/68
CPC分类号： C12N15/1034 , C12Q1/6806
摘要： A process for separating covalent DNA/protein complexes from non-covalent DNA/protein complexes and unbound DNA comprising the steps of: (i) treating the DNA/protein complexes with a reactive derivative of polyethyleneglycol (PEG) and (ii) subjecting the production of step (i) to phase partition between an aqueous PEG solution phase and an aqueous phosphate solution phase.
摘要翻译：从非共价DNA /蛋白质复合物和未结合的DNA分离共价DNA /蛋白质复合物的方法，包括以下步骤：（i）用聚乙二醇（PEG）的活性衍生物处理DNA /蛋白质复合物，和（ii）步骤（i）的相对分配在PEG水溶液相和磷酸盐水溶液相之间进行相分离。

10. 发明申请

WO1997043311A1 ANTICOAGULANT PEPTIDE FRAGMENTS DERIVED FROM APOLIPOPROTEIN B-100 审中-公开
标题翻译：来自APOLIPOPROTEIN B-100的抗皱胶原蛋白片段
公开(公告)号：WO1997043311A1
公开(公告)日：1997-11-20
申请号：PCT/GB1997001255
申请日：1997-05-09
申请人： ROYAL FREE HOSPITAL SCHOOL OF MEDICINE , BRUCKDORFER, Karl, Richard , ETTELAIE, Camille
发明人： ROYAL FREE HOSPITAL SCHOOL OF MEDICINE
IPC分类号： C07K14/775
CPC分类号： C07K14/775 , A61K38/00
摘要： The present invention provides a peptide compound of formula Z -K-A-Q-X -K-K-N-K-H-R-H-S-X -T-Z [SEQ ID No. 1] where: X represents S or Y, X represents T or I, Z represents the N terminus of the peptide, or from 1 to 47 amino acids, Z represents the C terminus of the peptide, a terminal amide group, or from 1 to 77 amino acids; or a variant of this peptide which contains one or more internal deletions, insertions or substitutions and which substantially retains anti-coagulant properties of apoB-100.
摘要翻译：本发明提供式Z 1 -KQX 1 -KKNKHRHSX 2 -ZZ 2 [SEQ ID No.1]的肽化合物，其中：X 1表示S或Y，X 2，代表T或I，Z 1表示肽的N末端，或1至47个氨基酸，Z 2表示肽的末端，末端酰胺基或1至77个氨基酸; 或该肽的变体，其含有一个或多个内部缺失，插入或取代并且基本上保留了apoB-100的抗凝血性质。

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