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1. 发明申请

WO2017165397A1 GENETICALLY ENGINEERED MICROBES AND METHODS FOR PRODUCING CITRAMALATE 审中-公开
标题翻译：遗传工程微生物和生产CITRAMALATE的方法
公开(公告)号：WO2017165397A1
公开(公告)日：2017-09-28
申请号：PCT/US2017/023380
申请日：2017-03-21
申请人： UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC. , WU, Xianghao , EITEMAN, Mark
发明人： WU, Xianghao , EITEMAN, Mark
IPC分类号： C12N15/70 , C12N9/10 , C12P7/46
CPC分类号： C12P7/46 , C12N9/10 , C12N9/1025 , C12N9/1029 , C12N15/70 , C12Y203/01182 , C12Y203/03001
摘要： Provided herein is a genetically engineered microbe which accumulates citramalate. In one embodiment, the microbe includes an exogenous polynucleotide encoding a citramalate synthase which catalyzes the condensation of acetyl CoA and pyruvic acid. Optionally, the microbe also includes a second exogenous polynucleotide encoding a citrate synthase which catalyzes the condensation of acetyl CoA and oxaloacetate, and the citrate synthase activity in the microbe is reduced compared to a control microbe. In one embodiment, the citrate synthase includes at least one amino acid substitution in the acetyl-CoA binding pocket, the mobile loop, the NADH binding site, and the oxaloacetate binding site, or a combination thereof. Also provided herein are methods for using the genetically engineered microbe, including a method is for producing citramalate. The method can further include isolating the citramalate.
摘要翻译：本文提供了一种积聚柠苹果酸酯的基因工程微生物。在一个实施方案中，微生物包括编码柠檬酸苹果酸合酶的外源多核苷酸，其催化乙酰CoA和丙酮酸的缩合。任选地，微生物还包括编码柠檬酸合酶的第二外源多核苷酸，其催化乙酰CoA和草酰乙酸的缩合，并且微生物中的柠檬酸合酶活性与对照微生物相比降低。在一个实施方案中，柠檬酸合酶在乙酰-CoA结合袋，移动环，NADH结合位点和草酰乙酸结合位点或其组合中包含至少一个氨基酸取代。本文还提供了使用基因工程微生物的方法，包括生产柠苹酸的方法。该方法可以进一步包括分离柠檬酸苹果酸酯。

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