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2. 发明申请

US20160237419A1 HUMAN TRYPSINOGEN WITH REDUCED AUTOACTIVATION AND ITS USE IN AN IMMUNOASSAY 有权
标题翻译：具有降低自动机能的人类免疫调节因子及其在免疫中的应用
公开(公告)号：US20160237419A1
公开(公告)日：2016-08-18
申请号：US15026604
申请日：2014-09-25
申请人： UNIVERSITÄT LEIPZIG
发明人： Karin BUTTNER , Agneta PRASSE , Thole ZUCHNER
IPC分类号： C12N9/76 , G01N33/542 , C12Q1/37
CPC分类号： C12N9/6427 , C12Q1/37 , C12Y304/21004 , G01N33/542
摘要： The present invention relates to a polypeptide consisting of or comprising a variant of human trypsinogen-1, comprising the substitutions: amino acid residue E64 is replaced with an amino acid residue comprising a positively charged side chain, amino acid residue K123 is replaced with an amino acid residue comprising an aliphatic side chain and amino acid residues Y139 and D147 are replaced with a glutamine or asparagine residue, and wherein said variant is further characterized in that: an amino acid residue selected from E16, E17 and E142 is replaced with an amino acid residue comprising an aliphatic side chain, and/or amino acid residue N18 is replaced with a histidine residue, and/or amino acid residue R107 is replaced with a lysine residue, and/or amino acid residue D138 is replaced with an amino acid residue comprising a positively charged side chain, and wherein said variant is cleavable into a polypeptide having a native-like enzymatic activity when compared to human trypsin-1.
摘要翻译：本发明涉及由人胰蛋白酶原-1的变体组成或包含人胰蛋白酶原-1变体的多肽，其包含取代：氨基酸残基E64被包含带正电侧链的氨基酸残基替代，氨基酸残基K123被氨基酸包含脂肪族侧链的酸残基和氨基酸残基Y139和D147被谷氨酰胺或天冬酰胺残基替代，并且其中所述变体的特征还在于：选自E16，E17和E142的氨基酸残基被氨基酸包含脂族侧链的残基和/或氨基酸残基N18被组氨酸残基取代，和/或氨基酸残基R107被赖氨酸残基替代，和/或氨基酸残基D138被氨基酸残基替代，带正电的侧链，并且当与人胰蛋白酶-1相比时，所述变体可切割成具有天然样酶活性的多肽。

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