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1. 发明申请

WO2013006755A3 SIMULTANEOUS SACCHARIFICATION AND FERMENTATION(SSF) OF LIGNOCELLULOSIC BIOMASS FOR SINGLE CELL OIL PRODUCTION BY OLEAGINOUS MICROORGANISMS 审中-公开
标题翻译：油脂微生物单细胞油生产的同源生物量的同时测定和发酵（SSF）
公开(公告)号：WO2013006755A3
公开(公告)日：2013-05-02
申请号：PCT/US2012045668
申请日：2012-07-06
申请人： UNIV WASHINGTON STATE RES FDN , YU XIAOCHEN , ZENG JIJLAO , ZHENG YUBIN , BULE MAHESH , CHEN SHULIN
发明人： YU XIAOCHEN , ZENG JIJLAO , ZHENG YUBIN , BULE MAHESH , CHEN SHULIN
IPC分类号： C12P7/64 , C12N1/14 , C12N1/16 , C12N9/24 , C12P19/02
CPC分类号： C12P7/6409 , C12P7/6463 , C12P2203/00 , Y02E50/343
摘要： Methods and systems for producing lipids which may be used as biofuel are provided. Sugars produced by pretreatment of lignocellulosic biomass are utilized by heterotrophic oleaginous fungi or yeast to increase their biomass and to produce lipids without prior detoxification of the pretreated biomass. After the fungi/yeast are cultured with the sugars, solid residues from the pretreated biomass are combined with the fungi/yeast under conditions which allow simultaneous 1) enzymatic degradation of cellulose and/or hemicellulose to produce sugars and 2) fermentation of the sugars for further increases in biomass and lipid production.
摘要翻译：提供了可用作生物燃料的生产脂质的方法和系统。由异养油性真菌或酵母通过预处理木质纤维素生物质产生的糖增加其生物量并产生脂质，而不预先处理的生物质的解毒。在将真菌/酵母与糖一起培养后，来自预处理的生物质的固体残余物与真菌/酵母在允许同时进行1）酶促降解纤维素和/或半纤维素以产生糖的条件下进行组合，以及2）发酵糖生物量和脂质生产进一步增加。

2. 发明申请

WO2010014797A3 INTEGRATED SYSTEM FOR PRODUCTIOIN OF BIOFUEL FEEDSTOCK 审中-公开
标题翻译：生物燃料原料生产综合系统
公开(公告)号：WO2010014797A3
公开(公告)日：2010-04-29
申请号：PCT/US2009052234
申请日：2009-07-30
申请人： UNIV WASHINGTON , CHI ZHANYOU , ZHENG YUBIN , LUCKER BEN , CHEN SHULIN
发明人： CHI ZHANYOU , ZHENG YUBIN , LUCKER BEN , CHEN SHULIN
IPC分类号： C12P7/00 , C02F3/32 , C12P1/00 , C12R1/89
CPC分类号： C12P7/649 , C02F3/32 , C12M21/02 , C12M23/18 , C12M23/58 , C12M41/06 , C12M41/12 , C12M41/34 , C12M47/06 , C12N1/12 , C12P7/6463 , Y02E50/13 , Y02W10/37
摘要： Disclosed is a culture system for the production of algae biomass to obtain lipid, protein and carbohydrate. By integrating heterotrophic processes with a phototrophic process in parallel, this system provides year around production in colder climates. By integrating heterotrophic processes with a phototrophic process in series, this system creates a two-stage, separated mixed- trophic algal process that uses organic carbon and nutrients for the production of seed in the heterotrophic process, followed by release of cultured seed in large-scale phototrophic culture for cell biomass accumulation. Organic carbon source including waste materials can be used to feed the heterotrophic process. The production capacity ratio between the heterotrophic and the phototrophic processes can be adjusted according to season and according to the availability of related resources. The systems are used for producing and harvesting an algal biofuel feedstock as well as other potential high-value products. The sequence and approach enhances utilization of carbon and nutrient waste-streams, provides an effective method for controlling contamination, adds flexibility in regard to production and type of available products, and supplies greater economic viability due to maximized use of available growth surface areas.
摘要翻译：公开了用于生产藻类生物质以获得脂质，蛋白质和碳水化合物的培养系统。通过将异养过程与光养过程并行整合，该系统在寒冷气候中提供了一年生产周期。通过将异养过程与光养过程串联整合，该系统创建了一个两阶段，分离的混合营养藻类过程，利用有机碳和营养物质在异养过程中生产种子，然后在大规模培养基中释放培养的种子，用于细胞生物量积累的大规模光养培养。包括废物在内的有机碳源可以用来喂养异养过程。异养和光养过程之间的生产能力比例可以根据季节和根据相关资源的可用性进行调整。该系统用于生产和收获藻类生物燃料原料以及其他潜在的高价值产品。序列和方法提高了碳和营养废物流的利用率，为控制污染提供了有效方法，增加了可用产品的生产和类型方面的灵活性，并由于最大限度地利用现有生长表面积而提供更大的经济可行性。

3. 发明公开

EP2734628A4 SIMULTANEOUS SACCHARIFICATION AND FERMENTATION(SSF) OF LIGNOCELLULOSIC BIOMASS FOR SINGLE CELL OIL PRODUCTION BY OLEAGINOUS MICROORGANISMS 审中-公开
标题翻译：同时糖化和发酵（SSF）木质纤维素生物质来生产EINZELLENÖLBY含油生物
公开(公告)号：EP2734628A4
公开(公告)日：2015-06-10
申请号：EP12808052
申请日：2012-07-06
申请人： UNIV WASHINGTON STATE RES FDN
发明人： YU XIAOCHEN , ZENG JIJLAO , ZHENG YUBIN , BULE MAHESH , CHEN SHULIN
IPC分类号： C12P7/64 , C12N1/14 , C12N1/16 , C12N9/24 , C12P19/02
CPC分类号： C12P7/6409 , C12P7/6463 , C12P2203/00 , Y02E50/343

4. 发明专利

CA2840632A1 SIMULTANEOUS SACCHARIFICATION AND FERMENTATION (SSF) OF LIGNOCELLULOSIC BIOMASS FOR SINGLE CELL OIL PRODUCTION BY OLEAGINOUS MICROORGANISMS 未知
公开(公告)号：CA2840632A1
公开(公告)日：2013-01-10
申请号：CA2840632
申请日：2012-07-06
申请人： UNIV WASHINGTON STATE RES FDN
发明人： YU XIAOCHEN , ZENG JIJLAO , ZHENG YUBIN , BULE MAHESH , CHEN SHULIN
IPC分类号： C12P7/64 , C12N1/14 , C12N1/16 , C12N9/24 , C12P19/02
摘要： Methods and systems for producing lipids which may be used as biofuel are provided. Sugars produced by pretreatment of lignocellulosic biomass are utilized by heterotrophic oleaginous fungi or yeast to increase their biomass and to produce lipids without prior detoxification of the pretreated biomass. After the fungi/yeast are cultured with the sugars, solid residues from the pretreated biomass are combined with the fungi/yeast under conditions which allow simultaneous 1) enzymatic degradation of cellulose and/or hemicellulose to produce sugars and 2) fermentation of the sugars for further increases in biomass and lipid production.

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