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1. 发明申请

WO2004038038A3 AMPLICON MELTING ANALYSIS WITH SATURATION DYES 审中-公开
标题翻译： AMPLICON熔融分析与饱和染料
公开(公告)号：WO2004038038A3
公开(公告)日：2004-07-22
申请号：PCT/US0333429
申请日：2003-10-22
申请人： UNIV UTAH RES FOUND , IDAHO TECHNOLOGY INC , WITTWER CARL T , DUJOLS VIRGINIE E , REED GUDRUN , ZHOU LUMING
发明人： WITTWER CARL T , DUJOLS VIRGINIE E , REED GUDRUN , ZHOU LUMING
IPC分类号： C09B23/04 , C12Q1/68 , C07D279/16 , C12P19/34
CPC分类号： C09B23/04 , C07D413/06 , C07D417/06 , C07H21/04 , C12Q1/6827 , C12Q1/6844 , C12Q1/6858 , C12Q1/686 , C12Q2527/125 , C12Q2527/107 , C12Q2565/107 , C12Q2565/101 , C12Q2537/107 , C12Q2537/165 , C12Q2545/113
摘要： Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of the dsDNA binding dye, and in another embodiment a melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.
摘要翻译：提供了用于核酸分析的方法，其中将至少部分双链的靶核酸与具有至少50％饱和百分比的dsDNA结合染料混合以形成混合物。在一个实施方案中，在dsDNA结合染料存在下扩增核酸，而在另一个实施方案中，通过在加热混合物时测量来自dsDNA结合染料的荧光，产生针对核酸的解链曲线。还提供了用于核酸分析的染料和用于制备染料的方法。

2. 发明公开

EP1558762A4 AMPLICON MELTING ANALYSIS WITH SATURATION DYES 有权转让
标题翻译：具有饱和染料扩增FUSION分析
公开(公告)号：EP1558762A4
公开(公告)日：2008-06-25
申请号：EP03777776
申请日：2003-10-22
申请人： UNIV UTAH RES FOUND , IDAHO TECHNOLOGY INC
发明人： WITTWER CARL T , DUJOLS VIRGINIE E , REED GUDRUN , ZHOU LUMING
IPC分类号： C09B23/04 , C12Q1/68 , C07D279/16 , C12P19/34
CPC分类号： C09B23/04 , C07D413/06 , C07D417/06 , C07H21/04 , C12Q1/6827 , C12Q1/6844 , C12Q1/6858 , C12Q1/686 , C12Q2527/125 , C12Q2527/107 , C12Q2565/107 , C12Q2565/101 , C12Q2537/107 , C12Q2537/165 , C12Q2545/113
摘要： Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of the dsDNA binding dye, and in another embodiment a melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.

3. 发明专利

CA2501144C AMPLICON MELTING ANALYSIS WITH SATURATION DYES 未知
公开(公告)号：CA2501144C
公开(公告)日：2015-10-06
申请号：CA2501144
申请日：2003-10-22
申请人： UNIV UTAH RES FOUND , IDAHO TECHNOLOGY INC
发明人： WITTWER CARL T , DUJOLS VIRGINIE E , REED GUDRUN , ZHOU LUMING
IPC分类号： C12Q1/68 , C07D279/16 , C09B23/04 , C12P19/34
摘要： Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of the dsDNA binding dye, and in another embodiment a melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.

4. 发明专利

CA2501144A1 AMPLICON MELTING ANALYSIS WITH SATURATION DYES 未知
公开(公告)号：CA2501144A1
公开(公告)日：2004-05-06
申请号：CA2501144
申请日：2003-10-22
申请人： UNIV UTAH RES FOUND , IDAHO TECHNOLOGY INC
发明人： DUJOLS VIRGINIE E , WITTWER CARL T , ZHOU LUMING , REED GUDRUN
IPC分类号： C09B23/04 , C12Q1/68 , C07D279/16 , C12P19/34
摘要： Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of the dsDNA bindi ng dye, and in another embodiment a melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.

5. 发明专利

ES2527068T3 Análisis de fusión de amplicones con colorantes de saturación 未知
公开(公告)号：ES2527068T3
公开(公告)日：2015-01-19
申请号：ES12165014
申请日：2003-10-22
申请人： UNIV UTAH RES FOUND , BIOFIRE DEFENSE LLC
发明人： WITTWER CARL T , DUJOLS VIRGINIE E , REED GUDRUN , ZHOU LUMING
IPC分类号： C12Q1/68 , C07D279/16 , C09B23/04 , C12P19/34
摘要： Procedimiento de análisis PCR que comprende las etapas de: mezclar un colorante de unión de ADNbc con una muestra que comprende un ácido nucleico diana y cebadores seleccionados, configurados para amplificar el ácido nucleico diana seleccionado, amplificar el ácido nucleico diana en presencia del colorante de unión de ADNbc, monitorizar la fluorescencia del colorante de unión de ADNbc, en el que la etapa de monitorización comprende la fusión del ácido nucleico diana amplificado para generar una curva de fusión, repetir la mezcla, amplificar y generar etapas de una curva de mezcla con, como mínimo, un ácido nucleico diana adicional y comparar las curvas de fusión, en el que la curva de fusión para el ácido nucleico diana seleccionado es seleccionada como estándar y es trazada como estándar a través de las temperaturas de fusión y la curva de fusión para cada ácido nucleico diana adicional es trazada como diferencia de fluorescencia del estándar a través de las temperaturas de fusión, y utilizando la diferencia trazada entre el estándar y cada uno de los ácidos nucleicos diana adicionales para identificar genotipos de cada ácido nucleico diana.

6. 发明专利

ES2438967T3 Análisis de fusión de amplicones con colorantes de saturación 未知
公开(公告)号：ES2438967T3
公开(公告)日：2014-01-21
申请号：ES03777776
申请日：2003-10-22
申请人： UNIV UTAH RES FOUND , IDAHO TECHNOLOGY INC , BIOFIRE DIAGNOSTICS INC
发明人： WITTWER CARL T , DUJOLS VIRGINIE E , REED GUDRUN , ZHOU LUMING
IPC分类号： C12Q1/68 , C07D279/16 , C09B23/04 , C12P19/34
摘要： Procedimiento de análisis por PCR que comprende las etapas de: proporcionar una mezcla de un colorante de unión a ADNbc y cebadores configurados para amplificar el ácidonucleico diana, amplificar el ácido nucleico diana en presencia del colorante de unión a ADNbc, monitorizar la fluorescencia del colorante de unión a ADNbc, generar una curva de fusión para el ácido nucleico diana, normalizar las diferencias de magnitud de la curva de fusión, repetir las etapas de provisión, amplificación, normalización y generación con, como mínimo, un ácido nucleico dianaadicional, representar gráficamente una diferencia de fluorescencia entre las curvas de fusión normalizadas, en el que la curvade fusión normalizada de un ácido nucleico diana se selecciona como patrón y se representa gráficamente comopatrón en temperaturas y las curvas de fusión normalizadas para ácido nucleico diana adicional se representagráficamente como una diferencia con las temperaturas en el patrón; y utilizar la diferencia representada gráficamente entre las curvas de fusión normalizadas para identificar genotiposde los ácidos nucleicos diana.

7. 发明专利

AU2003286573B2 Amplicon melting analysis with saturation dyes 未知
公开(公告)号：AU2003286573B2
公开(公告)日：2009-09-10
申请号：AU2003286573
申请日：2003-10-22
申请人： IDAHO TECHNOLOGY INC , UNIV UTAH RES FOUND
发明人： ZHOU LUMING , WITTWER CARL T , DUJOLS VIRGINIE E , REED GUDRUN
IPC分类号： C12Q1/68 , C09B23/04
摘要： The invention provides: A kit for amplifying and subsequently melting a plurality of target nucleic acids comprising: (a) amplification reagents; (b) a dsDNA binding dye having a percent saturation of at least about 90%; (c) information on amplifying each of the plurality of target nucleic acids in the presence of the dsDNA binding dye using the amplification reagents, to generate a plurality of amplified nucleic acids; and (d) information on generating a fluorescence melting curve from each of the plurality of amplified nucleic acids and plotting the difference between each of the fluorescence melting curves. The invention also provides a method of PCR analysis comprising the steps of: providing replicates of a mixture of a dsDNA binding dye, a target nucleic acid having a specific genotype, and primers configured for amplifying the target nucleic acid, amplifying each of the replicates of the target nucleic acid in the presence of the dsDNA binding dye, monitoring fluorescence of the dsDNA binding dye, generating a melting curve for each of the replicates of the target nucleic acid, repeating the providing, amplifying and generating steps with at least one additional target nucleic acid, establishing the target nucleic acid melting curve as a standard across temperatures using the replicates of the target nucleic acid having the specific genotype, and plotting a fluorescence difference between the standard and the melting curve of the at least one additional target nucleic acid.

8. 发明专利

AU2003286573A1 AMPLICON MELTING ANALYSIS WITH SATURATION DYES 未知
公开(公告)号：AU2003286573A1
公开(公告)日：2004-05-13
申请号：AU2003286573
申请日：2003-10-22
申请人： UNIV UTAH RES FOUND
发明人： REED GUDRUN , ZHOU LUMING , WITTWER CARL T , DUJOLS VIRGINIE E
IPC分类号： C09B23/04
摘要： The invention provides: A kit for amplifying and subsequently melting a plurality of target nucleic acids comprising: (a) amplification reagents; (b) a dsDNA binding dye having a percent saturation of at least about 90%; (c) information on amplifying each of the plurality of target nucleic acids in the presence of the dsDNA binding dye using the amplification reagents, to generate a plurality of amplified nucleic acids; and (d) information on generating a fluorescence melting curve from each of the plurality of amplified nucleic acids and plotting the difference between each of the fluorescence melting curves. The invention also provides a method of PCR analysis comprising the steps of: providing replicates of a mixture of a dsDNA binding dye, a target nucleic acid having a specific genotype, and primers configured for amplifying the target nucleic acid, amplifying each of the replicates of the target nucleic acid in the presence of the dsDNA binding dye, monitoring fluorescence of the dsDNA binding dye, generating a melting curve for each of the replicates of the target nucleic acid, repeating the providing, amplifying and generating steps with at least one additional target nucleic acid, establishing the target nucleic acid melting curve as a standard across temperatures using the replicates of the target nucleic acid having the specific genotype, and plotting a fluorescence difference between the standard and the melting curve of the at least one additional target nucleic acid.

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