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    • 2. 发明申请
    • METHODS AND SYSTEM FOR CONFOCAL LIGHT SCATTERING SPECTROSCOPIC IMAGING
    • 用于共焦光散射光谱成像的方法和系统
    • WO2010075385A3
    • 2010-10-14
    • PCT/US2009069196
    • 2009-12-22
    • UNIV TUFTSGEORGAKOUDI IRENEHUANG PONG-YUHUNTER MARTIN
    • GEORGAKOUDI IRENEHUANG PONG-YUHUNTER MARTIN
    • G01N23/203G01B11/24G01N21/47
    • G01B11/24A61B5/0059A61B5/0068G01J3/4412G01N21/31G01N21/474G02B21/0064G02B21/0092
    • The present invention is generally directed to imaging methods and apparatus that employ angular and/or wavelength distribution of light backscattered from multiple portions of a sample in response to illumination by electromagnetic radiation to generate one, two or three dimensional images of the sample. In many embodiments, confocal imaging can be employed to detect the backscattered radiation, e.g., to measure spectral signals of layered samples (such as biological samples) through optical sectioning. The methods of the invention can be applied to a variety of samples including, without limitation, biological and non-biological samples, organic and inorganic samples, to obtain information, e.g., regarding morphological, compositional, and/or structural variations among different portions of the sample. By way of example, in some applications the methods of invention can be employed to obtain light scattering signals from cells or tissues buried under the skin.
    • 本发明一般涉及成像方法和装置,其采用来自样品的多个部分的反向散射的光的角度和/或波长分布,以响应于电磁辐射的照射而产生样品的一维,二维或三维图像。 在许多实施例中,可以采用共焦成像来检测反向散射辐射,例如通过光学切片来测量分层样品(例如生物样品)的光谱信号。 本发明的方法可以应用于各种样品,包括但不限于生物和非生物样品,有机和无机样品,以获得关于例如关于不同部分之间的形态,组成和/或结构变化的信息 例子。 作为示例,在一些应用中,可以采用本发明的方法来从掩埋在皮肤下的细胞或组织获得光散射信号。