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1. 发明申请

WO2007068313A2 TARGETING AND TRACING OF ANTIGENS IN LIVING CELLS 审中-公开
标题翻译：抗生素在生活细胞中的靶向和追踪
公开(公告)号：WO2007068313A2
公开(公告)日：2007-06-21
申请号：PCT/EP2006010872
申请日：2006-11-13
申请人： UNIV MUENCHEN L MAXIMILIANS , ROTHBAUER ULRICH , LEONHARDT HEINRICH
发明人： ROTHBAUER ULRICH , LEONHARDT HEINRICH
IPC分类号： C07K19/00 , C07K16/46
CPC分类号： G01N33/566 , C07K1/22 , C07K14/43595 , C07K16/18 , C07K2317/22 , C07K2317/92 , C07K2319/60
摘要： The present invention relates to a method of detecting the presence, amount or subcellular location of an antigenic structure of interest in a cell, comprising the steps of: (a) (i) expressing a fusion protein directed to the antigenic structure of interest in said cell or (ii) introducing a fusion protein directed to the antigenic structure of interest and coupled to a (poly)peptide capable of transducing into said cell; wherein said fusion protein comprises a first (poly)peptide sequence comprising the variable region of a heavy chain antibody of Camelidae and a second (poly)peptide sequence derivable from a fluorescent or chromophoric protein.
摘要翻译：本发明涉及一种检测感兴趣的抗原结构在细胞中的存在，数量或亚细胞位置的方法，包括以下步骤：（a）（i）（i）表达针对所述抗原结构的融合蛋白，引入针对感兴趣的抗原结构并与能够转导入所述细胞的（多）肽偶联的融合蛋白; 其中所述融合蛋白包含第一（多）肽序列，其包含骆驼科的重链抗体的可变区和可衍生自荧光或发色蛋白的第二（多）肽序列。

2. 发明专利

DK2055718T3 MÅLSØGNING OG SPORING AF ANTIGENER I LEVENDE CELLER 未知
公开(公告)号：DK2055718T3
公开(公告)日：2013-04-02
申请号：DK08019358
申请日：2006-11-13
申请人： UNIV MUENCHEN L MAXIMILIANS
发明人： ROTHBAUER ULRICH , LEONHARDT HEINRICH
IPC分类号： C07K16/18 , C07K16/46 , C07K19/00

3. 发明专利

AU2010270304A1 Detection and visualization of the cell cycle in living cells 未知
公开(公告)号：AU2010270304A1
公开(公告)日：2012-02-02
申请号：AU2010270304
申请日：2010-07-06
申请人： UNIV MUENCHEN L MAXIMILIANS
发明人： ROTHBAUER ULRICH , LEONHARDT HEINRICH , ZOLGHADR KOUROSH , ROMER TINA , SCHMIDTHALS KATRIN
IPC分类号： C07K16/18 , C07K14/415 , C12N15/13 , C12N15/62 , C12N15/63 , G01N33/53
摘要： The present invention relates to a nucleic acid molecule encoding a polypeptide specifically binding to proliferating cell nuclear antigen (PCNA), said nucleic acid molecule comprising a nucleic acid sequence encoding the amino acid sequence of SEQ ID NO: 2 or an amino acid sequence deviating from SEQ ID NO: 2 by conservative substitution of one or more amino acids in position 1 to 28, 38 to 52, 63 to 98 and 115 to 123 of SEQ ID NO: 2. The present invention also relates to a vector comprising the nucleic acid molecule of the invention a host cell comprising the nucleic acid molecule of or the vector of the invention and a method of detecting the amount and/or location of PCNA in living cells, a method of screening for compounds having an effect on the cell cycle.

4. 发明专利

CA2766856A1 DETECTION AND VISUALIZATION OF THE CELL CYCLE IN LIVING CELLS 未知
公开(公告)号：CA2766856A1
公开(公告)日：2011-01-13
申请号：CA2766856
申请日：2010-07-06
申请人： UNIV MUENCHEN L MAXIMILIANS
发明人： ROTHBAUER ULRICH , LEONHARDT HEINRICH , ZOLGHADR KOUROSH , ROMER TINA , SCHMIDTHALS KATRIN
IPC分类号： C07K16/18 , C07K14/415 , C12N15/13 , C12N15/62 , C12N15/63 , G01N33/53
摘要： The present invention relates to a nucleic acid molecule encoding a polypeptide specifically binding to proliferating cell nuclear antigen (PCNA), said nucleic acid molecule comprising a nucleic acid sequence encoding the amino acid sequence of SEQ ID NO: 2 or an amino acid sequence deviating from SEQ ID NO: 2 by conservative substitution of one or more amino acids in position 1 to 28, 38 to 52, 63 to 98 and 115 to 123 of SEQ ID NO: 2. The present invention also relates to a vector comprising the nucleic acid molecule of the invention a host cell comprising the nucleic acid molecule of or the vector of the invention and a method of detecting the amount and/or location of PCNA in living cells, a method of screening for compounds having an effect on the cell cycle.

5. 发明专利

ES2401359T3 Direccionamiento y seguimiento de antígenos en células vivas 未知
公开(公告)号：ES2401359T3
公开(公告)日：2013-04-18
申请号：ES08019358
申请日：2006-11-13
申请人： UNIV MUENCHEN L MAXIMILIANS
发明人： ROTHBAUER ULRICH , LEONHARDT HEINRICH
IPC分类号： C07K16/18 , C07K16/46 , C07K19/00
摘要： Un polipéptido que comprende la región variable una cadena pesada de un anticuerpo de Camelidae y capaz deunirse específicamente a un epítopo de GFP, que consiste en o comprende: marco 1, CDR1, marco 2, CDR2, marco3 y CDR3, (a) que tiene la secuencia de una cualquiera de las SEQ ID NOs: 10 o 30 o está codificado por la molécula deácido nucleico de una cualquiera de las SEQ ID NOs: 11 o 31; o (b) que está codificado por una molécula de ácido nucleico con al menos un 95% de identidad de secuencia conla SEQ ID NO: 11.

6. 发明专利

AU2009203825A1 A fluorescent teo-hybrid (F2H) assay for direct visualization of protein interactions in living 未知
公开(公告)号：AU2009203825A1
公开(公告)日：2009-07-16
申请号：AU2009203825
申请日：2009-01-08
申请人： UNIV MUENCHEN L MAXIMILIANS
发明人： ZOLGHADR KOUROSH , ROTHBAUER ULRICH , LEONHARDT HEINRICH , MORTUSEWICZ OLIVER
IPC分类号： C12N15/10 , C12Q1/68 , G01N33/542
摘要： The present invention relates to an in vitro method for detecting protein-protein interactions comprising: (a) expressing in a eukaryotic cell a first fusion protein comprising (i) a (poly)peptide that, when expressed in a cell, accumulates at distinct sites in the nucleus of the cell or interacts with proteinaceous or non-proteinaceous structures accumulated at distinct sites in the nucleus of the cell; and (ii) a (poly)peptide specifically binding to GFP; (b) expressing in the same cell a second fusion protein comprising (i) GFP; and (ii) a bait (poly)peptide; (c) expressing in the same cell a third fusion protein comprising (i) a fluorescent (poly)peptide, the excitation and/or emission wavelength of which differs from that of GFP; and (ii) a prey (poly)peptide; and (d) detecting the fluorescence emission of the fluorescent parts of the second and the third fusion protein in the cell upon excitation, wherein a co-localization of the fluorescence emission of both fusion proteins in the cell nucleus is indicative of an interaction of the bait and the prey (poly)peptide. The invention also relates to an in vitro method for detecting protein-protein interactions comprising: (a) expressing in a eukaryotic cell a first fusion protein comprising (i) a fluorescent (poly)peptide; (ii) a (poly)peptide that, when expressed in a cell, accumulates at distinct sites in the nucleus of the cell; and (iii) a bait (poly)peptide (b) expressing in the same cell a second fusion protein comprising (i) a fluorescent (poly)peptide, the excitation and/or emission wavelength of which differs from that of the fluorescent (poly)peptide comprised in said first fusion protein; and (ii) a prey (poly)peptide and (c) detecting the fluorescence emission of the fluorescent parts of the first and the second fusion protein in the cell upon excitation, wherein a co-localization of the fluorescence emission of both fusion proteins in the cell nucleus is indicative of an interaction of the bait and the prey (poly)peptide. Furthermore, the present invention relates to methods for identifying a compound modulating the interaction of two (poly)peptides and methods of determining the relative strength of the interaction of two proteins with a third protein.

7. 发明专利

CA2628388A1 TARGETING AND TRACING OF ANTIGENS IN LIVING CELLS 未知
公开(公告)号：CA2628388A1
公开(公告)日：2007-06-21
申请号：CA2628388
申请日：2006-11-13
申请人： UNIV MUENCHEN L MAXIMILIANS
发明人： ROTHBAUER ULRICH , LEONHARDT HEINRICH
IPC分类号： C07K19/00 , C07K16/46
摘要： The present invention relates to a method of detecting the presence, amount or subcellular location of an antigenic structure of interest in a cell, comprising the steps of : (a) (i) expressing a fusion protein directed to the antigenic structure of interest in said cell or (ii) introducing a fusion protein directed to the antigenic structure of interest and coupled to a (poly) peptide capable of transducing into said cell; wherein said fusion protein comprises a first (poly) peptide sequence comprising the variable region of a heavy chain antibody of Camelidae and a second (poly) peptide sequence derivable from a fluorescent or chromophoric protein.

8. 发明专利

CA2709701A1 A FLUORESCENT TEO-HYBRID (F2H) ASSAY FOR DIRECT VISUALIZATION OF PROTEIN INTERACTIONS IN LIVING 未知
公开(公告)号：CA2709701A1
公开(公告)日：2009-07-16
申请号：CA2709701
申请日：2009-01-08
申请人： UNIV MUENCHEN L MAXIMILIANS
发明人： ROTHBAUER ULRICH , LEONHARDT HEINRICH , ZOLGHADR KOUROSH , MORTUSEWICZ OLIVER
IPC分类号： C12N15/10 , C12Q1/68 , G01N33/542
摘要： The present invention relates to an in vitro method for detecting protein-protein interactions comprising: (a) expressing in a eukaryotic cell a first fusion protein comprising (i) a (poly)peptide that, when expressed in a cell, accumulates at distinct sites in the nucleus of the cell or interacts with proteinaceous or non-proteinaceous structures accumulated at distinct sites in the nucleus of the cell; and (ii) a (poly)peptide specifically binding to GFP; (b) expressing in the same cell a second fusion protein comprising (i) GFP; and (ii) a bait (poly)peptide; (c) expressing in the same cell a third fusion protein comprising (i) a fluorescent (poly)peptide, the excitation and/or emission wavelength of which differs from that of GFP; and (ii) a prey (poly)peptide; and (d) detecting the fluorescence emission of the fluorescent parts of the second and the third fusion protein in the cell upon excitation, wherein a co-localization of the fluorescence emission of both fusion proteins in the cell nucleus is indicative of an interaction of the bait and the prey (poly)peptide. The invention also relates to an in vitro method for detecting protein-protein interactions comprising: (a) expressing in a eukaryotic cell a first fusion protein comprising (i) a fluorescent (poly)peptide; (ii) a (poly)peptide that, when expressed in a cell, accumulates at distinct sites in the nucleus of the cell; and (iii) a bait (poly)peptide (b) expressing in the same cell a second fusion protein comprising (i) a fluorescent (poly)peptide, the excitation and/or emission wavelength of which differs from that of the fluorescent (poly)peptide comprised in said first fusion protein; and (ii) a prey (poly)peptide and (c) detecting the fluorescence emission of the fluorescent parts of the first and the second fusion protein in the cell upon excitation, wherein a co- localization of the fluorescence emission of both fusion proteins in the cell nucleus is indicative of an interaction of the bait and the prey (poly)peptide. Furthermore, the present invention relates to methods for identifying a compound modulating the interaction of two (poly)peptides and methods of determining the relative strength of the interaction of two proteins with a third protein.

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