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1. 发明申请

WO2008091288A3 MICROBIAL SYNTHESIS OF D-1,2,4-BUTANETRIOL 审中-公开
标题翻译： D-1,2,4-BUTANETRIOL的微观合成
公开(公告)号：WO2008091288A3
公开(公告)日：2009-03-19
申请号：PCT/US2007016384
申请日：2007-07-19
申请人： UNIV MICHIGAN STATE , FROST JOHN W , NIU WEI
发明人： FROST JOHN W , NIU WEI
IPC分类号： C12P7/18 , C07C31/22 , C07K16/40 , C12N9/04 , C12N9/88 , C12Q1/68
CPC分类号： C12P7/18 , C12N9/0006 , C12N9/0008 , C12N9/88
摘要： Improved enzyme systems, recombinant cells, and processes employing the same to produce biosynthetic D-1,2,4-butanetriol; D-1,2,4-butanetriol prepared thereby and derivatives thereof; D-1,2,4-butanetriol trinitrate prepared therefrom; and enzymes and genes useful in the enzyme systems and recombinant cells.
摘要翻译：改进的酶系统，重组细胞和使用其的方法生产生物合成D-1,2,4-丁三醇的方法; 由此制备的D-1,2,4-丁三醇及其衍生物; 由其制备的D-1,2,4-丁三醇三硝酸酯; 以及可用于酶系统和重组细胞的酶和基因。

2. 发明申请

WO2006060646A3 CELL MICROARRAY FOR PROFILING OF CELLULAR PHENOTYPES AND GENE FUNCTION 审中-公开
标题翻译：细胞微阵列分析细胞表型和基因功能
公开(公告)号：WO2006060646A3
公开(公告)日：2006-09-08
申请号：PCT/US2005043600
申请日：2005-12-03
申请人： UNIV TEXAS , MARCOTTE EDWARD , IYER VISHWANATH , ELLINGTON ANDREW , DAVIES JONATHON , NIU WEI , SCOURAS ALEX , NARAYANASWAMY ROMMOHAN
发明人： MARCOTTE EDWARD , IYER VISHWANATH , ELLINGTON ANDREW , DAVIES JONATHON , NIU WEI , SCOURAS ALEX , NARAYANASWAMY ROMMOHAN
IPC分类号： C12Q1/02 , G01N21/76 , G06K9/00
CPC分类号： G01N33/502 , C12Q1/025 , G01N33/5008 , G01N33/5026 , G01N33/5041 , G01N33/542 , G01N33/582
摘要： The present invention includes compositions, methods and systems for analyzing one or more cell characteristics including the steps of depositing one or more spots on a substrate that include one or more cells from a strain collection, contacting the one or more cells with one or more agents and evaluating optically effect of the agent on the one or more cells.
摘要翻译：本发明包括用于分析一种或多种细胞特征的组合物，方法和系统，所述组合物，方法和系统包括以下步骤：在包含来自应变集合的一个或多个细胞的基底上沉积一个或多个斑点，使所述一个或多个细胞与一种或多种试剂以及评估试剂在一个或多个细胞上的光学效应。

3. 发明专利

BRPI0714439A2 未知
公开(公告)号：BRPI0714439A2
公开(公告)日：2014-04-08
申请号：BRPI0714439
申请日：2007-07-19
申请人： UNIV MICHIGAN STATE
发明人： FROST JOHN W , NIU WEI
IPC分类号： C12P7/18 , C07C31/22 , C07K16/40 , C12N9/04 , C12N9/88 , C12Q1/68
摘要： Improved enzyme systems, recombinant cells, and processes employing the same to produce biosynthetic D-1,2,4-butanetriol; D-1,2,4-butanetriol prepared thereby and derivatives thereof; D-1,2,4-butanetriol trinitrate prepared therefrom; and enzymes and genes useful in the enzyme systems and recombinant cells.

4. 发明申请

WO2008115840A3 COMPOSITIONS AND METHODS FOR THE BIOSYNTHESIS OF 1,4-BUTANEDIOL AND ITS PRECURSORS 审中-公开
标题翻译： 1,4-丁二醇及其前体的生物合成的组合物和方法
公开(公告)号：WO2008115840A3
公开(公告)日：2009-01-29
申请号：PCT/US2008057168
申请日：2008-03-14
申请人： GENOMATICA INC , BURK MARK J , VAN DIEN STEPHEN J , BURGARD ANTHONY , NIU WEI
发明人： BURK MARK J , VAN DIEN STEPHEN J , BURGARD ANTHONY , NIU WEI
IPC分类号： C12P1/00
CPC分类号： C12N15/52 , C12N9/0006 , C12N9/0008 , C12N9/88 , C12N9/93 , C12N15/70 , C12N15/81 , C12P7/18 , C12P7/42 , C12P7/52 , C12P17/04 , C12Y101/01061 , C12Y102/01076 , C12Y401/01071 , C12Y602/01004 , Y02P20/52
摘要： The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or a-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, a-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4- butanediol (BDO). Additionally provided is a method for the production of 4-HB. The method includes culturing a non-naturally occurring microbial organism having a A- hydroxybutanoic acid (4-HB) biosynthetic pathway including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase or a-ketoglutarate decarboxylase under substantially anaerobic conditions for a sufficient period of time to produce monomeric A- hydroxybutanoic acid (4-HB). Further provided is a method for the production of BDO. The method includes culturing a non-naturally occurring microbial biocatalyst, comprising a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-hydroxybutyrate kinase, phosphotranshydroxybutyrylase, a-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase for a sufficient period of time to produce 1,4-butanediol (BDO). The 4-HB and/or BDO products can be secreted into the culture medium.
摘要翻译：本发明提供了一种非天然存在的微生物生物催化剂，其包括具有4-羟基丁酸（4-HB）生物合成途径的微生物生物，其具有至少一种编码4-羟基丁酸脱氢酶的外源核酸，琥珀酰辅酶A合成酶，CoA依赖性琥珀酸半醛脱氢酶或α-酮戊二酸脱羧酶，其中外源核酸以足够的量表达以产生单体4-羟基丁酸（4-HB）。还提供了非天然存在的微生物生物催化剂，其包括具有4-羟基丁酸（4-HB）和1,4-丁二醇（BDO）生物合成途径的微生物生物，该途径包括至少一种编码4-羟基丁酸脱氢酶的外源核酸，琥珀酰辅酶A合成酶，CoA依赖性琥珀酸半醛脱氢酶，4-羟基丁酸酯：CoA转移酶，4-丁酸酯激酶，磷酸转移酶，α-酮戊二酸脱羧酶，醛脱氢酶，醇脱氢酶或醛/醇脱氢酶，其中外源核酸为以足够的量表示产生1,4-丁二醇（BDO）。另外提供了生产4-HB的方法。该方法包括培养具有4-羟基丁酸（4-HB）生物合成途径的非天然存在的微生物生物，其包含至少一种编码4-羟基丁酸脱氢酶的外源核酸，琥珀酰辅酶A合成酶，辅酶A依赖性琥珀酸半醛脱氢酶或 - 酮戊二酸脱羧酶在充分的厌氧条件下持续足够的时间以产生单体的4-羟基丁酸（4-HB）。还提供了用于生产BDO的方法。该方法包括培养非天然存在的微生物生物催化剂，其包含具有4-羟基丁酸（4-HB）和1,4-丁二醇（BDO）生物合成途径的微生物生物，该途径包括至少一种编码4- 羟基丁酸脱氢酶，琥珀酰-CoA合成酶，CoA依赖性琥珀酸脱氢酶，4-羟基丁酸酯：CoA转移酶，4-羟基丁酸酯激酶，磷酸转移羟基丁酸酶，α-酮戊二酸脱羧酶，醛脱氢酶，醇脱氢酶或醛/醇脱氢酶，时间来生产1,4-丁二醇（BDO）。 4-HB和/或BDO产物可以分泌到培养基中。

5. 发明申请

WO2008089123A3 METHODS FOR ANALYSIS OF HEDGEHOG PATHWAY INHIBITORS 审中-公开
标题翻译： HEDGEHOG路径抑制剂分析方法
公开(公告)号：WO2008089123A3
公开(公告)日：2008-10-16
申请号：PCT/US2008050970
申请日：2008-01-14
申请人： INFINITY DISCOVERY INC , FAIA KERRIE L , MANDLEY EVERTON , NIU WEI , PIEN CHRISTINE S
发明人： FAIA KERRIE L , MANDLEY EVERTON , NIU WEI , PIEN CHRISTINE S
IPC分类号： C12P19/34
CPC分类号： C12Q1/6876 , C12Q2600/136 , C12Q2600/158
摘要： One aspect of the present invention relates to a method of ascertaining the inhibitory activity in a mammal of a candidate inhibitor of the hedgehog pathway. In certain embodiments, the candidate inhibitor is administered systemically. In certain embodiments, the mammal is a rodent or primate. In certain embodiments, the mammal is a mouse. In certain embodiments, the candidate inhibitor is a small molecule or natural product.
摘要翻译：本发明的一个方面涉及确定哺乳动物中刺猬蛋白通路候选抑制剂的抑制活性的方法。在某些实施方案中，系统地施用候选抑制剂。在某些实施方案中，哺乳动物是啮齿动物或灵长类动物。在某些实施方案中，哺乳动物是小鼠。在某些实施方案中，候选抑制剂是小分子或天然产物。

6. 发明申请

WO2015120459A3 LIVE, ATTENUATED VACCINES AND METHODS OF MAKING AND USING 审中-公开
标题翻译：活生物，衰老疫苗及其制备和使用方法
公开(公告)号：WO2015120459A3
公开(公告)日：2015-11-26
申请号：PCT/US2015015229
申请日：2015-02-10
申请人： NUTECH VENTURES , GUO JIANTAO , LI QINGSHENG , NIU WEI , LI YUE , WANG NANXI
发明人： GUO JIANTAO , LI QINGSHENG , NIU WEI , LI YUE , WANG NANXI
IPC分类号： A61P31/18 , C12N7/04 , C12N15/867
CPC分类号： A61K39/21 , A61K39/12 , A61K2039/5254 , C12N7/00 , C12N2740/16021 , C12N2740/16034 , C12N2740/16051 , C12N2740/16234
摘要： A live, attenuated HIV vaccine is provided, and methods of making a live, attenuated HIV vaccine are provided. In one aspect, a live, · attenuated HIV vaccine is provided, where the genome of the HIV includes at least one first mutation in an essential region. Typically, the first mutation is a nonsense mutation. In some embodiments, the genome of the HIV further comprises at least one transgene in a non-essential region, the transgene encoding a suppressor tRNA and a corresponding aminoacyl-tRNA synthetase.
摘要翻译：提供活的减毒HIV疫苗，并提供制作活的，减毒的HIV疫苗的方法。一方面，提供活的减毒HIV疫苗，其中HIV的基因组在必需区域中包括至少一个第一突变。通常，第一个突变是无义突变。在一些实施方案中，HIV的基因组还包含非必需区域中的至少一个转基因，编码抑制子tRNA的转基因和相应的氨酰-tRNA合成酶。

7. 发明申请

WO2010144746A3 MICROORGANISMS AND METHODS FOR CARBON-EFFICIENT BIOSYNTHESIS OF MEK AND 2-BUTANOL 审中-公开
标题翻译：用于碳氢化合物和2-丁醇的高效生物合成的微生物和方法
公开(公告)号：WO2010144746A3
公开(公告)日：2011-02-24
申请号：PCT/US2010038230
申请日：2010-06-10
申请人： GENOMATICA INC , OSTERHOUT ROBIN E , NIU WEI , BURGARD ANTHONY P
发明人： OSTERHOUT ROBIN E , NIU WEI , BURGARD ANTHONY P
IPC分类号： C12P7/26 , C12P7/16
CPC分类号： C12P7/26 , C12N15/52 , C12P7/16 , Y02E50/10
摘要： A non-naturally occurring microbial organism has at least one exogenous nucleic acid encoding a MEK pathway enzyme expressed in a sufficient amount to produce MEK. The MEK pathway includes an enzyme selected from an acetoacetyl-CoA dehydrogenase (bifunctional), an acetoacetyl-CoA aldehyde dehydrogenase, a 3-oxobutyraldehyde reductase, a 3-oxobutanol dehydratase, an MEK oxidoreductase, a 3-oxobutyraldehyde aminotransferase, a 4-aminobutan-2-one deaminase, a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP aminotransferase, a 2,4-dioxopentanoate decarboxylase, an AKP deaminase, an acetylacrylate decarboxylase, an AKP decarboxylase, a glutamate dehydrogenase, a 3-oxobutyraldehyde oxidoreductase (aminating) and an AKP oxidoreductase (aminating). A 2-butanol pathway further includes an MEK reductase. A method for producing MEK or 2-butanol includes culturing these organisms under conditions and for a sufficient period of time to produce MEK or 2-butanol.
摘要翻译：非天然存在的微生物有至少一种编码MEK途径酶的外源核酸，其表达量足以产生MEK。 MEK途径包括选自乙酰乙酰辅酶A脱氢酶（双功能），乙酰乙酰辅酶A醛脱氢酶，3-氧代丁醛还原酶，3-氧代丁醇脱水酶，MEK氧化还原酶，3-氧代丁醛氨基转移酶，4-氨基丁酸 -2-酮脱氨酶，2-氨基-4-酮戊酸酯（AKP）硫解酶，AKP氨基转移酶，2,4-二氧代戊酸脱羧酶，AKP脱氨酶，乙酰丙酸酯脱羧酶，AKP脱羧酶，谷氨酸脱氢酶，氧化丁醛氧化还原酶（胺化）和AKP氧化还原酶（胺化）。 2-丁醇途径还包括MEK还原酶。包括生产MEK或2-丁醇的方法包括在条件和足够的时间内培养这些生物体以产生MEK或2-丁醇。

8. 发明申请

WO2010141920A3 MICROORGANISMS FOR THE PRODUCTION OF 1,4-BUTANEDIOL AND RELATED METHODS 审中-公开
标题翻译：用于生产1,4-丁二醇的微生物及相关方法
公开(公告)号：WO2010141920A3
公开(公告)日：2011-01-27
申请号：PCT/US2010037544
申请日：2010-06-04
申请人： GENOMATICA INC , VAN DIEN STEPHEN J , BURGARD ANTHONY P , HASELBECK ROBERT , PUJOL-BAXLEY CATHERINE J , NIU WEI , TRAWICK JOHN D , YIM HARRY , BURK MARK J , OSTERHOUT ROBIN E , SUN JUN
发明人： VAN DIEN STEPHEN J , BURGARD ANTHONY P , HASELBECK ROBERT , PUJOL-BAXLEY CATHERINE J , NIU WEI , TRAWICK JOHN D , YIM HARRY , BURK MARK J , OSTERHOUT ROBIN E , SUN JUN
IPC分类号： C12P7/16
CPC分类号： C12P7/18 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/13 , C12N9/16 , C12N9/88 , C12N9/93 , C12N15/52
摘要： The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.
摘要翻译：本发明提供包含1,4-丁二醇（BDO）途径的非天然存在的微生物生物，其包含至少一种编码足够量的BDO途径酶的外源核酸以产生BDO并进一步优化BDO的表达。本发明还提供了使用这种微生物生产BDO的方法。

9. 发明申请

WO2009088442A3 ISOMERASES AND EPIMERASES AND METHODS OF USING 审中-公开
标题翻译：异构体和硫化物及其使用方法
公开(公告)号：WO2009088442A3
公开(公告)日：2010-03-18
申请号：PCT/US2008013968
申请日：2008-12-22
申请人： CARGILL INC , DE SOUZA MERVYN L , NIU WEI , LAPLAZA JOSE M , SOLHEID CHRISTOPHER , KOLLMANN SHERRY R , LUNDORFF JOSHUA M , HICKS PAULA M , MCFARLAN SARA C , SANCHEZ-RIERA FERNANDO A , WEINER DAVID P , BURKE ELLEN , LUGINBUHL PETER , BUENO ANALIA , CUENCA JOSLIN
发明人： DE SOUZA MERVYN L , NIU WEI , LAPLAZA JOSE M , SOLHEID CHRISTOPHER , KOLLMANN SHERRY R , LUNDORFF JOSHUA M , HICKS PAULA M , MCFARLAN SARA C , SANCHEZ-RIERA FERNANDO A , WEINER DAVID P , BURKE ELLEN , LUGINBUHL PETER , BUENO ANALIA , CUENCA JOSLIN
IPC分类号： C12Q1/68 , C12N9/90
CPC分类号： C12P13/04 , C12P13/227
摘要： The invention provides for isomerase (e.g., racemase) and epimerase polypeptides and nucleic acids encoding such polypeptides. Also provided are methods of using such isomerase (e.g., racemase) and epimerase nucleic acids and polypeptides.
摘要翻译：本发明提供异构酶（例如消旋酶）和差向异构酶多肽以及编码这种多肽的核酸。还提供了使用这种异构酶（例如消旋酶）和差向异构酶核酸和多肽的方法。

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