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1. 发明申请

WO2006033974A3 DETECTION OF PROTEIN AGGREGATES BY HOMOLOGOUS ELISA 审中-公开
标题翻译：通过同源ELISA检测蛋白质聚集体
公开(公告)号：WO2006033974A3
公开(公告)日：2007-07-12
申请号：PCT/US2005032985
申请日：2005-09-16
申请人： UNIV CASE WESTERN RESERVE , SY MAN-SUN , PAN TAO , LI RULIANG
发明人： SY MAN-SUN , PAN TAO , LI RULIANG
IPC分类号： G01N33/53
CPC分类号： G01N33/53 , G01N33/6896 , G01N2800/2828
摘要： A method of detecting disease related protein aggregate in a sample comprises using a capture monoclonal antibody and detecting-monoclonal antibody. The capture monoclonal antibody and the detecting monoclonal antibody are identical.
摘要翻译：检测样品中疾病相关蛋白聚集体的方法包括使用捕获单克隆抗体和检测单克隆抗体。捕获单克隆抗体和检测单克隆抗体是相同的。

2. 发明申请

WO2008070229A2 DETECTION OF PATHOGENIC AGGREGATES OF PROTEIN IN A SAMPLE BY HOMOLOGOUS ELISA 审中-公开
标题翻译：同源ELISA法检测样品中蛋白质的致病凝集物
公开(公告)号：WO2008070229A2
公开(公告)日：2008-06-12
申请号：PCT/US2007076986
申请日：2007-08-28
申请人： UNIV CASE WESTERN RESERVE , SY MAN-SUN , CHANG BINGGONG , LI CHAOYANG
发明人： SY MAN-SUN , CHANG BINGGONG , LI CHAOYANG
IPC分类号： G01N33/53
CPC分类号： G01N33/537 , G01N33/6896 , G01N2333/4709 , G01N2800/2821 , G01N2800/2828
摘要： A method of detecting pathogenic aggregates of a protein in a sample comprises contacting the sample with a first monoclonal antibody or epitope binding fragment thereof, the first monoclonal antibody or an epitope binding fragment thereof being immunoreactive with the protein of the pathogenic aggregates, removing pathogenic aggregates of the protein not bound to the first monoclonal antibody or epitope binding fragment thereof, contacting the bound pathogenic aggregates of the protein with a second monoclonal antibody or epitope binding fragment thereof, the second monoclonal antibody or epitope binding fragment thereof being substantially identical to the first monoclonal antibody or epitope binding fragment thereof and being coupled to a detectable amplification module, amplifying the amplification module coupled to the second monoclonal antibody bound to the pathogenic aggregates, and detecting the amount of amplified amplification module to determine the presence of protein aggregates in the sample.
摘要翻译：检测样品中蛋白质的致病性聚集体的方法包括使样品与第一单克隆抗体或其表位结合片段接触，第一单克隆抗体或其表位结合片段与致病性聚集体的蛋白质发生免疫反应，除去致病性聚集体未与第一单克隆抗体或其表位结合片段结合的蛋白质，使蛋白质的结合致病性聚集体与第二单克隆抗体或其表位结合片段接触，第二单克隆抗体或其表位结合片段与第一单克隆抗体或其表位结合片段基本上相同单克隆抗体或其表位结合片段并与可检测的扩增模块偶联，扩增偶联于与致病性聚集体结合的第二单克隆抗体的扩增模块，并检测扩增扩增模块的量以确定蛋白质聚集体样本中的门。

3. 发明申请

WO9966956A9 IMMUNOLOGICAL AGENTS SPECIFIC FOR PRION PROTEIN (PrP) 审中-公开
标题翻译：优质蛋白质（PrP）特异性免疫细胞
公开(公告)号：WO9966956A9
公开(公告)日：2000-05-11
申请号：PCT/US9914024
申请日：1999-06-22
申请人： UNIV CASE WESTERN RESERVE
发明人： SY MAN-SUN , GAMBETTI PIERLUIGI
IPC分类号： A61P31/00 , C07K14/47 , C07K16/18 , C12P21/04 , G01N33/68 , A61K39/395 , C07K16/00 , G01N33/537
CPC分类号： G01N33/6896 , C07K14/47 , C07K16/18 , C07K2317/73 , G01N2800/2828
摘要： The present invention is directed to a panel of monoclonal antibodies (Mabs) specific for murine prion protein PrP . These Mabs can be applied to immunoblotting, cell surface immunofluorescent staining and immunohistochemistry at light and electron microscopy. Additionally, these Mabs recognize both the normal (PrP ) and protease-resistant (PrP ) isoforms of PrP. Some Mabs are species restricted, while others react with PrP from a broad range of mammals including mice, humans, monkeys, cows, sheep, squirrels and hamsters. Moreover, several of the Mabs selectively recognize different PrP glycoforms as well as the metabolic fragments of PrP . These newly generated PrP antibodies are useful for exploring the biology of PrP and to establish the diagnosis of prion diseases in both humans and animals.
摘要翻译：本发明涉及一种对鼠朊蛋白PrP c特异的单克隆抗体（Mabs）。这些Mabs可以应用于免疫印迹，细胞表面免疫荧光染色和免疫组织化学在光和电子显微镜。此外，这些Mabs既识别PrP的正常（PrP ）和蛋白酶抗性（PrP ）同种型。一些Mab是物种限制的，而其他Mab与来自广泛哺乳动物（包括小鼠，人类，猴子，牛，绵羊，松鼠和仓鼠）的PrP反应。此外，几种Mab选择性地识别不同的PrP糖型以及PrP 的代谢片段。这些新产生的PrP 抗体可用于探索PrP 的生物学和建立人类和动物中朊病毒疾病的诊断。

4. 发明公开

EP1800125A4 DETECTION OF PROTEIN AGGREGATES BY HOMOLOGOUS ELISA 审中-公开
标题翻译：蛋白质聚集体同源ELISA检验检测
公开(公告)号：EP1800125A4
公开(公告)日：2009-04-29
申请号：EP05797841
申请日：2005-09-16
申请人： UNIV CASE WESTERN RESERVE
发明人： SY MAN-SUN , PAN TAO , LI RULIANG
IPC分类号： G01N33/53 , G01N33/68
CPC分类号： G01N33/53 , G01N33/6896 , G01N2800/2828
摘要： A method of detecting disease related protein aggregate in a sample comprises using a capture monoclonal antibody and detecting-monoclonal antibody. The capture monoclonal antibody and the detecting monoclonal antibody are identical.

5. 发明专利

AU4957499A Immunological agents specific for prion protein (prp) 未知
公开(公告)号：AU4957499A
公开(公告)日：2000-01-10
申请号：AU4957499
申请日：1999-06-22
申请人： UNIV CASE WESTERN RESERVE
发明人： SY MAN-SUN , GAMBETTI PIERLUIGI
IPC分类号： A61P31/00 , C07K14/47 , C07K16/18 , C12P21/04 , G01N33/68 , A61K39/395 , C07K16/00 , G01N33/537

6. 发明申请

WO2005021711A3 APPROACH FOR OBTAINING MONOCLONAL ANTIBODIES TO CELL SURFACE PROTEINS 审中-公开
标题翻译：用于获得单克隆抗体对细胞表面蛋白的方法
公开(公告)号：WO2005021711A3
公开(公告)日：2005-05-26
申请号：PCT/US2004019217
申请日：2004-06-16
申请人： RES FOUND MENTAL HYGIENE , RUBENSTEIN RICHARD , KASCSAK RICHARD , KIM JAE-IL , KURIZON SOLOMON , PETERSEN ROBERT , SY MAN-SUN
发明人： RUBENSTEIN RICHARD , KASCSAK RICHARD , KIM JAE-IL , KURIZON SOLOMON , PETERSEN ROBERT , SY MAN-SUN
IPC分类号： C07K16/18 , C07K16/28 , C12N20060101 , C12N5/18 , C12N5/20 , C12P21/08
CPC分类号： C07K16/2872
摘要： The present invention provides a novel method for producing monoclonal antibodies against a variety of mammalian antigens, and in particular, cell surface antigens that are relatively conserved between species. In a specific embodiment, the invention relates to the generation of monoclonal antibodies that specifically react with prion proteins (PrP). The method comprises combining a PrP knockout mouse and a PrP-non-expressing fusion partner for hybridoma production, thereby opening all regions and conformation of the molecule to antibody production. This approach leads to many new and useful monoclonal antibodies of varying specificities.
摘要翻译：本发明提供了产生针对多种哺乳动物抗原的单克隆抗体的新方法，特别是在物种间相对保守的细胞表面抗原。在具体的实施方案中，本发明涉及与朊病毒蛋白（PrP）特异性反应的单克隆抗体的产生。该方法包括将PrP敲除小鼠和PrP-非表达融合伴侣组合用于杂交瘤生产，从而打开分子的所有区域和构象以产生抗体。这种方法导致许多具有不同特异性的新的和有用的单克隆抗体。

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