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1. 发明授权

US07632618B2 Polyhydroxyalkanoate-containing structure and manufacturing method thereof 失效
标题翻译：含聚羟基链烷酸酯的结构及其制造方法
公开(公告)号：US07632618B2
公开(公告)日：2009-12-15
申请号：US12177094
申请日：2008-07-21
申请人： Tsuyoshi Nomoto , Tetsuya Yano , Shinya Kozaki , Tsutomu Honma
发明人： Tsuyoshi Nomoto , Tetsuya Yano , Shinya Kozaki , Tsutomu Honma
IPC分类号： C03G9/00 , C08G63/06 , C12N11/14 , C12P7/40
CPC分类号： C12N9/1025 , C12N11/00 , C12P7/625 , Y10T428/2989
摘要： A method for manufacturing polyhydroxyalkanoate-containing structure, at least a part of a base material surface of the structure being coated with polyhydroxyalkanoate, the method comprises the steps of immobilizing a polyhydroxyalkanoate synthase on the base material surface, synthesizing, on the base material surface, polyhydroxyalkanoate using a 3-hydroxyacyl coenzyme A to become the substrate of the synthase and the synthase and coating at least a part of the base material surface with the synthesized polyhydroxyalkanoate, wherein the synthase contains an amino acid sequence capable of binding to the base material. A polyhydroxyalkanoate-containing structure, at least a part of a base material surface of the structure being coated with a polyhydroxyalkanoate, comprises the base material, a polyhydroxyalkanoate synthase immobilized on the base material surface, and the polyhydroxyalkanoate with which at least a part of the base material surface is coated, wherein the synthase contains an amino acid sequence capable of binding to the base material.
摘要翻译：一种制备聚羟基链烷酸酯结构的方法，所述结构的基材表面的至少一部分涂覆有聚羟基链烷酸酯，所述方法包括以下步骤：将聚羟基链烷酸酯合酶固定在基材表面上，在基材表面上合成，聚羟基链烷酸酯使用3-羟酰基辅酶A成为合成酶和合成酶的底物并用合成的聚羟基链烷酸酯涂覆至少一部分基材表面，其中合成酶含有能够与基材结合的氨基酸序列。一种含聚羟基链烷酸酯的结构，该结构的基材表面的至少一部分涂覆有聚羟基链烷酸酯，其包含基材，固定在基材表面上的聚羟基链烷酸酯合酶和至少一部分的聚羟基链烷酸酯基材表面被涂覆，其中合成酶含有能够结合基材的氨基酸序列。

2. 发明授权

US07632600B2 Enzyme electrode and method of producing the same 失效
标题翻译：酶电极及其制造方法
公开(公告)号：US07632600B2
公开(公告)日：2009-12-15
申请号：US11525848
申请日：2006-09-25
申请人： Wataru Kubo , Tsuyoshi Nomoto , Tetsuya Yano , Rie Ueno
发明人： Wataru Kubo , Tsuyoshi Nomoto , Tetsuya Yano , Rie Ueno
IPC分类号： H01M4/00 , H01M4/88 , C12N11/00
CPC分类号： H01M8/16 , H01M4/8803 , H01M4/8817 , H01M4/8828 , H01M4/8882 , H01M4/9008 , Y02E60/527
摘要： An enzyme electrode that enables to enhance the rate of charge transfer from a redox center of an enzyme and catalytic current is provided. An enzyme electrode capable of increasing catalytic current by increasing the rate of charge transfer from an enzyme using an enzyme/metal fine particle complex in which part of a metal fine particle is incorporated into the enzyme can be provided.
摘要翻译：提供能够提高从酶的氧化还原中心的电荷转移速率和催化电流的酶电极。可以提供能够通过增加使用其中部分金属微粒掺入到酶中的酶/金属微粒络合物从酶的电荷转移速率来增加催化电流的酶电极。

3. 发明授权

US07524659B2 Isogenic strain line of bacterium for producing polyhydroxyalkanoate in which polyhydroxyalkanoate synthase gene is disrupted and method for producing polyhydroxyalkanoate using the same 失效
标题翻译：用于产生聚羟基链烷酸酯合酶基因被破坏的聚羟基链烷酸酯的细菌的同基因株系和使用其的聚羟基链烷酸酯的制备方法
公开(公告)号：US07524659B2
公开(公告)日：2009-04-28
申请号：US11341495
申请日：2006-01-30
申请人： Tsuyoshi Nomoto , Tetsuya Yano
发明人： Tsuyoshi Nomoto , Tetsuya Yano
IPC分类号： C12P7/62 , C12N9/00 , C12N1/20 , C07H21/04
CPC分类号： C12R1/38 , C12N9/1029
摘要： A host-vector system which is equipped with a substrate supply system enzyme for polyhydroxyalkanoate synthase and which is suitable for evolutionary engineering modification of polyhydroxyalkanoate synthase. An isogenic strain line is produced by disrupting a polyhydroxyalkanoate synthase gene of a bacterium for producing polyhydroxyalkanoate.
摘要翻译：一种宿主载体系统，其配备有用于聚羟基链烷酸酯合成酶的底物供应系统酶，并且其适用于聚羟基链烷酸酯合酶的进化工程修饰。通过破坏用于制备聚羟基链烷酸酯的细菌的聚羟基链烷酸酯合酶基因来产生同基因株系。

4. 发明申请

US20080108123A1 MAGNETIC SUBSTANCE-BIOSUBSTANCE COMPLEX STRUCTURE, PEPTIDE FRAGMENT CAPABLE OF LINKING TO MAGNETIC SUBSTANCE AND GENE THEREFOR, AND PROCESS FOR PRODUCING THE COMPLEX STRUCTURE 审中-公开
标题翻译：磁性物质 - 生物传感器复合结构，能够连接到磁性物质及其基因的肽片段，以及用于生产复合结构的方法
公开(公告)号：US20080108123A1
公开(公告)日：2008-05-08
申请号：US11937611
申请日：2007-11-09
申请人： Takeshi Imamura , Tetsuya Yano , Tsuyoshi Nomoto , Shinya Kozaki , Tsutomu Honma , Akiko Tsuchitani
发明人： Takeshi Imamura , Tetsuya Yano , Tsuyoshi Nomoto , Shinya Kozaki , Tsutomu Honma , Akiko Tsuchitani
IPC分类号： C12N9/00 , C07H23/00 , C11C3/00 , C07K1/00 , C07H21/04 , C07K14/00
CPC分类号： C12N9/1025 , C07K2319/00 , C12N11/06 , C12N11/14 , C12N15/1013 , G01N33/54326 , G01N33/54353 , Y10S530/811
摘要： A magnetic substance-biosubstance complex structure comprises a magnetic substance-containing carrier and a biosubstance immobilized on the carrier, the biosubstance being immobilized through a spacer comprising an amino acid sequence on a surface of the carrier.
摘要翻译：磁性物质 - 生物物质复合物结构包括含磁性物质的载体和固定在载体上的生物体，生物质通过包含载体表面上的氨基酸序列的间隔物固定化。

5. 发明授权

US07235396B2 Bacterium for producing polyhydroxyalkanoate having polyhydroxyalkanoate depolymerase gene disrupted and method for producing polyhydroxyalkanoate using the same 失效
标题翻译：用于制备具有聚羟基链烷酸酯解聚酶基因的聚羟基链烷酸酯的破坏细菌和使用其的聚羟基链烷酸酯的制备方法
公开(公告)号：US07235396B2
公开(公告)日：2007-06-26
申请号：US11341665
申请日：2006-01-30
申请人： Tsuyoshi Nomoto , Tetsuya Yano
发明人： Tsuyoshi Nomoto , Tetsuya Yano
IPC分类号： C12N1/20 , C12P7/62 , C12Q3/07 , C12N15/74 , C12N1/21
CPC分类号： C12R1/38 , C12N9/18 , C12P7/625
摘要： The objective of the present invention is to provide a bacterium for producing polyhydroxyalkanoate with improved productivity. To achieve above objective, the gene coding for the polyhydroxyalkanoate depolymerase of the bacterium for producing polyhydroxyalkanoate is disrupted to improve the productivity of polyhydroxyalkanoate.
摘要翻译：本发明的目的是提供一种提高生产率的生产聚羟基链烷酸酯的细菌。为了达到上述目的，编码用于制备聚羟基链烷酸酯的细菌的聚羟基链烷酸酯解聚酶的基因被破坏以提高聚羟基链烷酸酯的生产率。

6. 发明申请

US20070131546A1 Enzyme electrode 审中-公开
标题翻译：酶电极
公开(公告)号：US20070131546A1
公开(公告)日：2007-06-14
申请号：US11634921
申请日：2006-12-07
申请人： Tsuyoshi Nomoto , Wataru Kubo , Tetsuya Yano
发明人： Tsuyoshi Nomoto , Wataru Kubo , Tetsuya Yano
IPC分类号： G01N33/487 , H01M4/90
CPC分类号： C12Q1/001 , C12Q1/32 , H01M4/9008 , H01M8/16 , Y02E60/527
摘要： There is provided with an enzyme electrode which can be used as a sensor with high sensitivity, a biofuel cell with high output, and an electrochemical reaction device with high reaction efficiency. The enzyme electrode has a conductive base plate, a fusion protein immobilized to the conductive base plate and an electron transfer mediator, wherein the fusion protein is a fusion protein of a enzyme 1 to catalyze a chemical reaction for producing a reaction product 1 from a reaction substrate 1 and a enzyme 2 to catalyze a chemical reaction for producing a reaction product 2 from a reaction substrate 2, and at least one chemical substance of the reaction product 1 is identical to at least one chemical substance of the reaction substrate 2.
摘要翻译：提供了可用作具有高灵敏度的传感器，高产量的生物燃料电池的酶电极和具有高反应效率的电化学反应装置。酶电极具有导电性基板，固定在导电性基板上的融合蛋白质和电子转移介质，其中融合蛋白是酶1的融合蛋白，用于催化从反应产生反应产物1的化学反应底物1和酶2以催化从反应基材2制备反应产物2的化学反应，并且反应产物1的至少一种化学物质与反应基底2的至少一种化学物质相同。

7. 发明申请

US20070056852A1 Enzyme electrode sensor fuel cell and electrochemical reactor 审中-公开
标题翻译：酶电极传感器燃料电池和电化学反应器
公开(公告)号：US20070056852A1
公开(公告)日：2007-03-15
申请号：US10573179
申请日：2005-07-22
申请人： Wataru Kubo , Tetsuya Yano , Tsuyoshi Nomoto
发明人： Wataru Kubo , Tetsuya Yano , Tsuyoshi Nomoto
IPC分类号： C12Q1/00 , H01M4/90
CPC分类号： C12Q1/004
摘要： An enzyme electrode comprises a conductive member and an enzyme, wherein a first mediator and a second mediator are immobilized by a carrier onto the conductive member, the first mediator and the second mediator having different redox potentials (reduction-oxidation potentials). A sensor employs the enzyme electrode as a detection portion for detecting a substance. A fuel cell employs the enzyme electrode as at least one of anode and a cathode. An electrochemical reactor employs the enzyme electrode as a reaction electrode.
摘要翻译：酶电极包括导电构件和酶，其中第一介体和第二介体通过载体固定在导电构件上，第一介体和第二介体具有不同的氧化还原电位（还原氧化电位）。传感器使用酶电极作为检测物质的检测部。燃料电池使用酶电极作为阳极和阴极中的至少一个。电化学反应器采用酶电极作为反应电极。

8. 发明申请

US20060172400A1 Bacterium for producing polyhydroxyalkanoate having polyhydroxyalkanoate depolymerase gene disrupted and method for producing polyhydroxyalkanoate using the same 失效
标题翻译：用于制备具有聚羟基链烷酸酯解聚酶基因的聚羟基链烷酸酯的破坏细菌和使用其的聚羟基链烷酸酯的制备方法
公开(公告)号：US20060172400A1
公开(公告)日：2006-08-03
申请号：US11341665
申请日：2006-01-30
申请人： Tsuyoshi Nomoto , Tetsuya Yano
发明人： Tsuyoshi Nomoto , Tetsuya Yano
IPC分类号： C12P7/62 , C12N15/74 , C12N1/21
CPC分类号： C12R1/38 , C12N9/18 , C12P7/625
摘要： The objective of the present invention is to provide a bacterium for producing polyhydroxyalkanoate with improved productivity. To achieve above objective, the gene coding for the polyhydroxyalkanoate depolymerase of the bacterium for producing polyhydroxyalkanoate is disrupted to improve the productivity of polyhydroxyalkanoate.
摘要翻译：本发明的目的是提供一种提高生产率的生产聚羟基链烷酸酯的细菌。为了达到上述目的，编码用于制备聚羟基链烷酸酯的细菌的聚羟基链烷酸酯解聚酶的基因被破坏以提高聚羟基链烷酸酯的生产率。

9. 发明申请

US20060172398A1 Isogenic strain line of bacterium for producing polyhydroxyalkanoate in which polyhydroxyalkanoate synthase gene is disrupted and method for producing polyhydroxyalkanoate using the same 失效
公开(公告)号：US20060172398A1
公开(公告)日：2006-08-03
申请号：US11341495
申请日：2006-01-30
申请人： Tsuyoshi Nomoto , Tetsuya Yano
发明人： Tsuyoshi Nomoto , Tetsuya Yano
IPC分类号： C12P7/62 , C12N1/21
CPC分类号： C12R1/38 , C12N9/1029
摘要： A host-vector system which is equipped with a substrate supply system enzyme for polyhydroxyalkanoate synthase and which is suitable for evolutionary engineering modification of polyhydroxyalkanoate synthase. An isogenic strain line is produced by disrupting a polyhydroxyalkanoate synthase gene of a bacterium for producing polyhydroxyalkanoate.

10. 发明授权

US06853477B2 Particles for electrophoresis, a production method thereof and a display using the particles 失效
标题翻译：用于电泳的颗粒，其制备方法和使用该颗粒的显示器
公开(公告)号：US06853477B2
公开(公告)日：2005-02-08
申请号：US10133405
申请日：2002-04-29
申请人： Tsuyoshi Nomoto , Tetsuya Yano , Shinya Kozaki , Tsutomu Honma
发明人： Tsuyoshi Nomoto , Tetsuya Yano , Shinya Kozaki , Tsutomu Honma
IPC分类号： G02F1/167 , C08G63/06 , C08G63/688 , C09B67/08 , C09C3/10 , C12P7/62 , G02B26/00 , A61K9/50 , G09G3/34 , G03G74/04
CPC分类号： B82Y30/00 , C01P2004/01 , C01P2004/61 , C01P2004/64 , C01P2004/84 , C01P2006/60 , C01P2006/66 , C01P2006/90 , C08G63/06 , C08G63/6882 , C09B67/0013 , C09C3/10 , C12P7/625 , Y10T428/24331 , Y10T428/2998
摘要： Electrophoretic particles for electrophoretic display having excellent dispersibility and dispersion stability with time for insulating media, and being protected against coagulation, settling and the like, a process for production of the electrophoretic particles having high versatility for pigments to be used in response to a full-color display, and an electrophoretic display device using the electrophoretic particles that has an excellent memory property and is highly reliable are provided. The electrophoretic particles are formed using as at least a part of structure a pigment with at least a part of the surface covered with polyhydroxyalkanoate.
摘要翻译：用于电泳显示的电泳颗粒具有优异的分散性和分散稳定性以及绝缘介质随时间的分散稳定性，并且可防止凝结，沉降等。制备电子粒子的方法具有高反应性全色的颜料的通用性，并且提供了使用具有优异记忆性并且高度可靠的电泳粒子的电泳显示装置。电泳颗粒使用颜料的至少一部分结构形成，其中至少一部分表面被聚羟基链烷酸酯覆盖。

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