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1. 发明专利

JP2006122042A Method for product certification 审中-公开
标题翻译：产品认证方法
公开(公告)号：JP2006122042A
公开(公告)日：2006-05-18
申请号：JP2005245807
申请日：2005-08-26
申请人： Tsunehiko Higuchi , Nissan Motor Co Ltd , 日産自動車株式会社 , 恒彦樋口
发明人： YOKOYAMA HIROSHI , YAMANAKA MASAHIKO , WATANABE KENTARO , HIGUCHI TSUNEHIKO , HAYASHI HIDETOSHI
IPC分类号： C12Q1/68 , C12N15/09
CPC分类号： C12Q1/6816 , G06F19/22 , C12Q2563/185 , C12Q2521/101
摘要： PROBLEM TO BE SOLVED: To provide a method for certifying a product by detecting an informational nucleic acid from the product containing the nucleic acid enabling the source and history of the product to be individually and definitely specified.
SOLUTION: The method for product certification comprises the following procedure: An informational nucleic acid having a site with an arbitrary and known base sequence is extracted from a product such as a coating material, resin, fat-and-oil, fiber, leather, wood, printed matter, paper or adhesive, or a combination thereof, and then amplified by PCR (polymer chain reaction) technique, thereafter, the base sequence of the informational nucleic acid thus amplified is determined.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供一种通过从包含核酸的产品中检测信息核酸以使得能够单独且明确地指定产品的来源和历史来验证产品的方法。解决方案：产品认证方法包括以下步骤：具有任意和已知碱基序列的位点的信息核酸从涂料，树脂，油脂，纤维等产品中提取，皮革，木材，印刷品，纸张或粘合剂或其组合，然后通过PCR（聚合物链反应）技术进行扩增，然后确定如此扩增的信息核酸的碱基序列。版权所有（C）2006，JPO＆NCIPI

2. 发明专利

JP2006094814A Information nucleic acid and information nucleic acid composition using the same 审中-公开
标题翻译：信息核酸和信息核酸组成使用相同
公开(公告)号：JP2006094814A
公开(公告)日：2006-04-13
申请号：JP2004286704
申请日：2004-09-30
申请人： Tsunehiko Higuchi , Nissan Motor Co Ltd , 日産自動車株式会社 , 恒彦樋口
发明人： YOKOYAMA HIROSHI , YAMANAKA MASAHIKO , WATANABE KENTARO , HIGUCHI TSUNEHIKO , HAYASHI HIDETOSHI
IPC分类号： C12N15/09 , H04L9/32
CPC分类号： C12N15/1003 , C12N15/10
摘要： PROBLEM TO BE SOLVED: To provide an information nucleic acid which can be added to an industrial product or the like to individually concretely specify the origin and history of the product, and to provide an information nucleic acid composition using the same. SOLUTION: This information nucleic acid is characterized by having a site with an arbitrary and known base sequence. The base sequence site is used for the amplification of the information nucleic acid. The information nucleic acid further has a certification information site. The base sequence is synthesized. The base sequence is a base sequence in which thymine units are not adjacent to each other. The base sequence has protecting groups for stabilization. The protecting groups include phosphoric ester groups, acyl groups, alkoxycarbonyl groups, benzyl groups, substituted benzyl groups and allyl groups. The 5'-hydroxy groups are derived with biotin or a fluorescent molecule. The information nucleic acid is mixed with additives such as a fat solubilizing agent, a heat-stabilizing agent, a photostabilizer, a hydrolase inhibitor, and a dispersant. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：待解决的问题：提供可以添加到工业产品等中以单独具体指定产品的来源和历史的信息核酸，并提供使用其的信息核酸组合物。解决方案：该信息核酸的特征在于具有任意和已知的碱基序列的位点。碱基序列位点用于扩增信息核酸。信息核酸还具有认证信息站点。合成碱基序列。碱基序列是胸腺嘧啶单元彼此不相邻的碱基序列。碱基序列具有用于稳定化的保护基。保护基包括磷酸酯基，酰基，烷氧基羰基，苄基，取代的苄基和烯丙基。 5'-羟基是用生物素或荧光分子衍生的。信息核酸与添加剂如脂肪增溶剂，热稳定剂，光稳定剂，水解酶抑制剂和分散剂混合。版权所有（C）2006，JPO＆NCIPI

3. 发明专利

JP2006094807A Informationized nucleic acid-carrying microparticle and method for producing the same 审中-公开
标题翻译：具有说明力的含有核酸的微生物及其生产方法
公开(公告)号：JP2006094807A
公开(公告)日：2006-04-13
申请号：JP2004286555
申请日：2004-09-30
申请人： Tsunehiko Higuchi , Nissan Motor Co Ltd , 日産自動車株式会社 , 恒彦樋口
发明人： HIGUCHI TSUNEHIKO , HAYASHI HIDETOSHI , YOKOYAMA HIROSHI , YAMANAKA MASAHIKO , WATANABE KENTARO
IPC分类号： C12N11/00 , C12N15/09
CPC分类号： C12Q1/6816 , C12Q2563/185 , C12Q2563/149
摘要： PROBLEM TO BE SOLVED: To provide informationized nucleic acid-carrying microparticles carrying an informationized nucleic acid used as information about source and history of a product, stably carrying the informationized nucleic acid in the product over a long period, preventing the outflow of the informationized nucleic acid and performing individual certification of the source and history over the long period and to provide a method for producing the informationized nucleic acid-carrying microparticles.
SOLUTION: The method for producing the informationized nucleic acid-carrying microparticles is carried out as follows. The informationized nucleic acid provided with a site having an optional and well-known base sequence and used as individual information about the source and history is directly added to a suspension of microparticles, e.g. silica or zinc white or a part or all of the informationized nucleic acid as a solution of sterile distilled water is added to the suspension. The resultant suspension is then dried. Thereby, the microparticles carrying the informationized nucleic acid on the surface are obtained. The resultant informationized nucleic acid-carrying microparticles are mixed in a raw material resin for, e.g. a coating or a resin molded product.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：为了提供携带信息化核酸的信息化携带核酸的信息，所述核酸用作关于产品的来源和历史的信息，在长时间内稳定地携带信息化的核酸在产品中，防止流出信息化核酸，并且长期进行源和历史的个体认证，并提供生产信息化携带核酸的微粒的方法。解决方案：用于生产信息化携带核酸的微粒的方法如下进行。提供有具有任选和公知碱基序列并用作关于来源和历史的个体信息的位点的信息化核酸被直接加入到微粒悬浮液中，例如，将二氧化硅或锌白色或作为无菌蒸馏水溶液的部分或全部信息化核酸加入到悬浮液中。然后将所得悬浮液干燥。由此，得到表面上载有信息化核酸的微粒。将得到的含有信息的携带核酸的微粒混合在原料树脂中，涂层或树脂模塑制品。版权所有（C）2006，JPO＆NCIPI

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