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1. 发明申请

US20030091221A1 Method for quantitative video-microscopy and associated system and computer software program product 有权
标题翻译：定量视频显微镜及相关系统和计算机软件程序产品的方法
公开(公告)号：US20030091221A1
公开(公告)日：2003-05-15
申请号：US09957446
申请日：2001-09-19
申请人： Tripath Imaging, Inc.
发明人： Raphael Marcelpoil , Thomas Gahm
IPC分类号： G06K009/00
CPC分类号： G06K9/00127 , G01N21/27 , G06T7/60 , G06T2207/10056
摘要： A method of determining an amount of at least one molecular specie comprising a sample is provided, each molecular specie being indicated by a dye. The amount of the molecular specie is determined from an image of the sample captured as image data by a color image acquisition device in a video-microscopy system. An optical density of the sample is first determined in each of a red, green, and blue channel at a particular pixel in the image. A corresponding optical density matrix is thereafter formed for that pixel. The optical density matrix is then multiplied by the inverse of a relative absorption coefficient matrix so as to form a resultant matrix for the pixel. The relative absorption coefficient matrix comprises a relative absorption coefficient for each dye, independently of the sample, in each of the red, green, and blue channels. The resultant matrix thus comprises the amount of each molecular specie, as indicated by the respective dye, for that pixel. Associated systems and computer software program products are also provided.
摘要翻译：提供了确定包含样品的至少一种分子物质的量的方法，每种分子物质由染料指示。通过视频显微镜系统中的彩色图像获取装置，从作为图像数据捕获的样本的图像确定分子种类的量。首先在图像中的特定像素处的红色，绿色和蓝色通道中的每一个中确定样本的光密度。此后为该像素形成对应的光密度矩阵。然后将光密度矩阵乘以相对吸收系数矩阵的倒数，以形成像素的合成矩阵。相对吸收系数矩阵包括在红色，绿色和蓝色通道中的每一个中独立于样品的每种染料的相对吸收系数。所得到的基质因此包含针对该像素的每种分子物质的量，如各染料所示。还提供相关系统和计算机软件程序产品。

2. 发明申请

US20030138140A1 Method for quantitative video-microscopy and associated system and computer software program product 有权
公开(公告)号：US20030138140A1
公开(公告)日：2003-07-24
申请号：US10057729
申请日：2002-01-24
申请人： TriPath Imaging, Inc.
发明人： Raphael Marcelpoil , Didier Morel
IPC分类号： G06K009/00
CPC分类号： G01N15/1475 , G01N21/31 , G01N2015/1472 , G06K9/0014 , G06K9/4652 , G06T7/0012 , G06T7/90 , G06T2200/24 , G06T2207/10056 , G06T2207/30024
摘要： A method of determining an amount of at least one molecular species in a sample from an image of the sample captured by an image acquisition device is provided, each molecular species being indicated by a dye. A dye space representation of a plurality of dyes is formed by orthogonally adding the correspondence tables of the dyes, each correspondence table having a plurality of normalized RGB triplets and incrementally extending from 0% to 100% transmittance. The dye space representation has one dimension for each dye and provides a reference model for a combination of the plurality of dyes. Each pixel of an image of the sample stained with the combination of the plurality of dyes is compared to the reference model, each pixel having a color defined by an RGB triplet, so as to determine an optimal combination of normalized RGB triplets from the respective correspondence tables of the dyes producing the color of the respective pixel. An artificial image of the sample is then formed from the normalized RGB triplets for each dye as determined from the optimal combination. The artificial image thereby indicates a distribution of the respective dye over the sample image and facilitates determination of the amount of the corresponding molecular species. Associated methods, systems, and computer software program products are also provided.

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