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1. 发明专利

JP2005127795A Well plate and manufacturing method therefor 审中-公开
标题翻译：井板及其制造方法
公开(公告)号：JP2005127795A
公开(公告)日：2005-05-19
申请号：JP2003362018
申请日：2003-10-22
申请人： Muranaka Iryoki Kk , Toyo Glass Co Ltd , 村中医療器株式会社 , 東洋ガラス株式会社
发明人： SASAKI MITSUO , YAMAZAKI MASATAKE
IPC分类号： G01N21/03 , C12M1/00 , C12M1/32 , C12M3/00
CPC分类号： C12M23/12
摘要： PROBLEM TO BE SOLVED: To develop a well plate for easily loading an object to be inspected, such as a cell and a chemical into each well, even if the diameter of the well is very much reduced and the number of wells is increased greatly.
SOLUTION: The well is formed on a resin layer formed on a glass surface, and the glass surface is exposed to the bottom section of the well. Since the surface of the resin layer and the inner surface of the well become hydrophobic, and the bottom surface of the well becomes hydrophilic, an object to be inspected selectively adheres to the bottom section of each well, when the object to be inspected is sprinkled, such as a cell onto a well plate. Then, when the surface is cleaned lightly by water or alcohol, the object to be inspected adhering to a part other than the well bottom section is easily removed, and the object to be inspected remains only at the bottom section of the well.
COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：为了开发一种井板，即使井的直径大大降低并且井的数量被减少，也容易将被检查物体（例如，单元和化学物质）加载到每个井中大大增加解决方案：在形成在玻璃表面上的树脂层上形成孔，并且玻璃表面暴露于井的底部。由于树脂层的表面和阱的内表面变得疏水，并且阱的底表面变为亲水性，当被检查物体被喷洒时，被检查对象选择性地粘附到每个孔的底部，例如细胞到孔板上。然后，当通过水或酒精轻轻地清洁表面时，容易去除附着在井底部分以外的部分的被检查物体，并且待检查的物体仅保留在井的底部。版权所有（C）2005，JPO＆NCIPI

2. 发明专利

JP2006266768A Well plate 审中-公开
标题翻译：井板
公开(公告)号：JP2006266768A
公开(公告)日：2006-10-05
申请号：JP2005083035
申请日：2005-03-23
申请人： Muranaka Iryoki Kk , Toyo Glass Co Ltd , 村中医療器株式会社 , 東洋ガラス株式会社
发明人： UEDA ATSUMI , SASAKI MITSUO , YAMAZAKI MASATAKE
IPC分类号： G01N21/03 , C12M1/00 , C12M1/34
CPC分类号： C12M41/36
摘要： PROBLEM TO BE SOLVED: To develop a well plate excellent in inspection efficiency, capable of preventing the drying of a culture medium or an inspection target and capable of easily receiving the inspection target in each of wells.
SOLUTION: The well plate improved in inspection efficiency can be constituted by providing a plurality of wells with a diameter of 1-500 μm and a depth of 1-300 μm on the surface of a substrate made of glass or a resin at an interval of 10-300 μm. A cover plate placing surface higher than a well-formed surface is formed in the periphery of the group of the wells and a cover plate is placed on the cover plate placing surface so as to cover the group of the wells to prevent the drying of the culture medium or the inspection target. If a metal film is formed on the inner surface of each of the wells and an alkanethiol film is formed on the metal film, the inspection target can be easily put in each of the wells.
COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：解决问题：为了开发出检查效率优异的孔板，能够防止培养基或检查对象的干燥，能够容易地接收各孔的检查对象。解决方案：检查效率提高的孔板可以通过在由玻璃或树脂制成的基板的表面上设置多个直径为1-500μm和深度为1-300μm的孔来构成间隔10-300μm。在一组井的周边形成盖板放置表面高于形成良好表面的盖板，并且将覆盖板放置在盖板放置表面上以覆盖井组，以防止干燥培养基或检查对象。如果在每个孔的内表面上形成金属膜，并且在金属膜上形成烷硫醇膜，则可以容易地将检查对象放入各孔中。版权所有（C）2007，JPO＆INPIT

3. 发明专利

JP2007212344A Well plate and testing system using well plate 审中-公开
标题翻译：井板和测试系统使用井板
公开(公告)号：JP2007212344A
公开(公告)日：2007-08-23
申请号：JP2006033921
申请日：2006-02-10
申请人： Toyo Glass Co Ltd , 東洋ガラス株式会社
发明人： UEDA ATSUMI , SASAKI MITSUO , YAMAZAKI MASATAKE
IPC分类号： G01N21/03
摘要： PROBLEM TO BE SOLVED: To easily ensure test object processing, such as seeding the test object to a well of well plate.
SOLUTION: The through hole is formed through the substrate from the surface to the rear face of the substrate, the through hole is blocked by sticking a transparent thin plate of 50 to 200μm thick, and a plurality of wells are formed on the upper face of the internal side of the through hole. Since the well is formed on the surface of the thin plate of 50 to 200μm thickness, the inspection objective body in the well can be observed from the rear side with an inverted microscope, therefore processing the inspection objects, such as seeding the inspection object can be performed easily and surely, without being obstructed by the objective lens of the microscope.
COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：为了容易地确保测试对象处理，例如将测试对象接种到井板的井中。解决方案：通孔从基板的表面到背面通过基板形成，通孔粘附50〜200μm厚的透明薄板，并在多个孔上形成多个孔通孔内侧的上表面。由于在厚度为50〜200μm的薄板的表面上形成了阱，因此可以用倒置的显微镜从背面观察井中的检查对象物体，因此对检查对象进行检查，例如检查对象的播种可以轻松，可靠地进行，而不会被显微镜的物镜遮挡。版权所有（C）2007，JPO＆INPIT

4. 发明专利

JP2006262740A Cell colonization and proliferation substrate 审中-公开
标题翻译：细胞结构和增殖基质
公开(公告)号：JP2006262740A
公开(公告)日：2006-10-05
申请号：JP2005083034
申请日：2005-03-23
申请人： Medgel Corp , Muranaka Iryoki Kk , Toyo Glass Co Ltd , 村中医療器株式会社 , 東洋ガラス株式会社 , 株式会社メドジェル
发明人： SASAKI MITSUO , YAMAZAKI MASATAKE
IPC分类号： C12M1/00 , C12N5/07 , C12N5/071 , C12N5/10
CPC分类号： C12M23/20 , C12M25/00
摘要： PROBLEM TO BE SOLVED: To provide a cell colonization and proliferation substrate suitable for various tests using cells and the separation, proliferation and differentiation of cells. SOLUTION: A coating film of a metal reactive with SH group is formed on the surface of a base material made of glass or a resin. An alkanethiol film is easily formable on the metal film reactive with SH group and, accordingly, a surface suitable for the adhesion and proliferation of cells can be formed by the method. The adhesion strength between the base material and the films can be improved by forming a contact/metal film layer between the surface of the base material and the film of the metal reactive with SH group. COPYRIGHT: (C)2007,JPO&INPIT

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