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1. 发明授权

US07256031B2 Enzyme having β-glucosidase activity and use thereof 有权
标题翻译：具有β-葡糖苷酶活性的酶及其用途
公开(公告)号：US07256031B2
公开(公告)日：2007-08-14
申请号：US10381434
申请日：2001-09-28
申请人： Tomoyuki Fukasawa , Chuhei Nojiri , Nobuo Matsuhashi , Koji Nishizawa , Kaoru Okakura , Takashi Yamanobe
发明人： Tomoyuki Fukasawa , Chuhei Nojiri , Nobuo Matsuhashi , Koji Nishizawa , Kaoru Okakura , Takashi Yamanobe
IPC分类号： C07H21/04 , C12N9/42 , C12N1/12 , C12N15/00 , C12P21/06
CPC分类号： C12Y302/01021 , A61K38/00 , C12N9/2445
摘要： The invention is to provide a novel β-glucosidase and a gene that codes for the enzyme, and to develop a technique of utilizing the β-glucosidase or a composition that contains the enzyme for processing plants or plant-derived substances.According to the invention, there are provided a novel enzyme showing a β-glucosidase activity and derived from filamentous fungi of the genus Acremonium, a gene that codes for the enzyme, a method of using the gene for expressing β-glucosidase, an enzyme composition that contains β-glucosidase, and a method of processing plants or plant-derived substances with the enzyme or the enzyme compositions.
摘要翻译：本发明提供一种新的β-葡糖苷酶和编码该酶的基因，并开发利用β-葡糖苷酶的技术或含有用于加工植物或植物衍生物质的酶的组合物。根据本发明，提供了一种显示β-葡糖苷酶活性的新型酶，其来自顶孢属的丝状真菌，编码该酶的基因，使用该基因表达β-葡糖苷酶的方法，酶组合物其含有β-葡糖苷酶，以及用酶或酶组合物处理植物或植物衍生物质的方法。

2. 发明申请

US20120100578A1 CELLULASE PREPARATION COMPRISING ENDOGLUCANASES DERIVED FROM TWO DIFFERENT TYPES OF MICROORGANISMS 有权
标题翻译：包含从两种不同类型的微生物衍生的内切酶的细胞因子制备
公开(公告)号：US20120100578A1
公开(公告)日：2012-04-26
申请号：US13381049
申请日：2010-07-01
申请人： Kaoru Okakura , Koichiro Murashima
发明人： Kaoru Okakura , Koichiro Murashima
IPC分类号： C12P19/00 , C12N9/42
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14 , C12Y302/01004 , D06M16/003
摘要： By having a cellulase preparation comprising at least a certain amount of endoglucanases derived from two different types of microorganisms, the cellulase preparation can be provided with a higher activity and a wider pH property than those of cellulase preparations each containing one of the endoglucanases alone. Moreover, by introducing and expressing simultaneously two different types of cellulase genes in a single host cell, a cellulase preparation having a high activity and a wide pH property can be produced easily.
摘要翻译：通过使含有至少一定量的衍生自两种不同类型的微生物的内切葡聚糖酶的纤维素酶制剂，纤维素酶制剂可以提供比单独含有一种内切葡聚糖酶的纤维素酶制剂更高的活性和更宽的pH特性。此外，通过在单个宿主细胞中同时引入和表达两种不同类型的纤维素酶基因，可以容易地制备具有高活性和宽pH特性的纤维素酶制剂。

3. 发明授权

US08975053B2 Thermostable catalase 有权
标题翻译：耐热过氧化氢酶
公开(公告)号：US08975053B2
公开(公告)日：2015-03-10
申请号：US12918017
申请日：2009-02-18
申请人： Kaoru Okakura , Fusuke Mazuka , Takayoshi Fukushima , Koichiro Murashima
发明人： Kaoru Okakura , Fusuke Mazuka , Takayoshi Fukushima , Koichiro Murashima
IPC分类号： C12N9/08 , C07H21/04
CPC分类号： C12N9/0065 , C12Y111/01006
摘要： An object is to efficiently produce thermostable catalase at low cost by expressing it as a recombinant protein in large quantity.A recombinant microorganism capable of efficiently expressing thermostable catalase can be provided by obtaining a DNA necessary for efficiently producing it as a recombinant protein, and the thermostable catalase can be efficiently produced at low cost by cultivating the obtained recombinant microorganism. Hydrogen peroxide can be efficiently decomposed at low cost, even at high temperature, by treating a solution containing hydrogen peroxide with the thermostable catalase of the present invention.
摘要翻译：目的是通过将其大量表达为重组蛋白，以低成本有效地产生热稳定的过氧化氢酶。可以通过获得作为重组蛋白质有效生产所必需的DNA来提供能够有效表达耐热性过氧化氢酶的重组微生物，通过培养所获得的重组微生物，可以低成本有效地制备热稳定性过氧化氢酶。通过用本发明的耐热性过氧化氢酶处理含有过氧化氢的溶液，即使在高温下也能以低成本有效地分解过氧化氢。

4. 发明授权

US07790430B2 Cellulase resistant to surfactants 有权
标题翻译：纤维素酶耐表面活性剂
公开(公告)号：US07790430B2
公开(公告)日：2010-09-07
申请号：US12101683
申请日：2008-04-11
申请人： Kaoru Okakura , Koji Yanai
发明人： Kaoru Okakura , Koji Yanai
IPC分类号： C12N9/24 , C12N9/42 , D06M16/00 , D21C1/00 , C12Q1/34 , A23L1/31 , C07H21/04
CPC分类号： C12N9/2437 , C11D3/38645 , C12Y302/01004 , D21C5/005 , D21H17/22
摘要： Disclosed is a novel cellulose having an amino acid sequence in which the 162nd and/or 166th amino acid residues in the amino acid sequence of cellulose NCE5 are substituted. Further, a polynucleotide encoding the novel cellulose, an expression vector containing the polynucleotide, a host cell transformed with the expression vector, and a cellulose preparation and a washing composition containing the cellulose are disclosed. The cellulose of the present invention is resistant to surfactants, and maintains a high activity even under alkaline conditions.
摘要翻译：公开了具有氨基酸序列的新型纤维素，其中纤维素NCE5的氨基酸序列中第162位和/或第166位氨基酸残基被取代。此外，公开了编码新型纤维素的多核苷酸，含有多核苷酸的表达载体，用表达载体转化的宿主细胞，以及含有纤维素的纤维素制剂和洗涤组合物。本发明的纤维素耐表面活性剂，即使在碱性条件下也能保持高活性。

5. 发明授权

US07374921B2 Cellulase tolerant to surfactants 有权
标题翻译：纤维素酶耐受表面活性剂
公开(公告)号：US07374921B2
公开(公告)日：2008-05-20
申请号：US10533310
申请日：2003-10-31
申请人： Kaoru Okakura , Koji Yanai
发明人： Kaoru Okakura , Koji Yanai
IPC分类号： C12N9/42 , C12N5/16 , C12Q1/34 , C12P21/06 , C07H21/04 , C11D3/00 , C07K1/00
CPC分类号： C12N9/2437 , C11D3/38645 , C12Y302/01004 , D21C5/005 , D21H17/22
摘要： Disclosed is a novel cellulase having an amino acid sequence in which the 162nd and/or 166th amino acid residues in the amino acid sequence of cellulase NCE5 are substituted. Further, a polynucleotide encoding the novel cellulase, an expression vector containing the polynucleotide, a host cell transformed with the expression vector, and a cellulase preparation and a washing composition containing the cellulase are disclosed. The cellulase of the present invention is resistant to surfactants, and maintains a high activity even under alkaline conditions.
摘要翻译：公开了具有氨基酸序列的新型纤维素酶，其中纤维素酶NCE5的氨基酸序列中的第162位和/或第166位氨基酸残基被取代。此外，公开了编码新型纤维素酶的多核苷酸，含有多核苷酸的表达载体，用表达载体转化的宿主细胞，以及含有该纤维素酶的纤维素酶制剂和洗涤组合物。本发明的纤维素酶对表面活性剂具有抗性，即使在碱性条件下也能保持高活性。

6. 发明申请

US20060035361A1 Novel cellulase tolerant to surfactants 有权
标题翻译：新型纤维素酶耐受表面活性剂
公开(公告)号：US20060035361A1
公开(公告)日：2006-02-16
申请号：US10533310
申请日：2003-10-31
申请人： Kaoru Okakura , Koji Yanai
发明人： Kaoru Okakura , Koji Yanai
IPC分类号： C11D3/386 , C07H21/04 , C12P21/06 , C12N9/24
CPC分类号： C12N9/2437 , C11D3/38645 , C12Y302/01004 , D21C5/005 , D21H17/22
摘要： Disclosed is a novel cellulase having an amino acid sequence in which the 162nd and/or 166th amino acid residues in the amino acid sequence of cellulase NCE5 are substituted. Further, a polynucleotide encoding the novel cellulase, an expression vector containing the polynucleotide, a host cell transformed with the expression vector, and a cellulase preparation and a washing composition containing the cellulase are disclosed. The cellulase of the present invention is resistant to surfactants, and maintains a high activity even under alkaline conditions.
摘要翻译：公开了具有氨基酸序列的新型纤维素酶，其中纤维素酶NCE5的氨基酸序列中的第162位和/或第166位氨基酸残基被取代。此外，公开了编码新型纤维素酶的多核苷酸，含有多核苷酸的表达载体，用表达载体转化的宿主细胞，以及含有该纤维素酶的纤维素酶制剂和洗涤组合物。本发明的纤维素酶对表面活性剂具有抗性，即使在碱性条件下也能保持高活性。

7. 发明授权

US09181537B2 Cellulase preparation comprising endoglucanases derived from two different types of microorganisms 有权
标题翻译：包含衍生自两种不同类型微生物的内切葡聚糖酶的纤维素酶制剂
公开(公告)号：US09181537B2
公开(公告)日：2015-11-10
申请号：US13381049
申请日：2010-07-01
申请人： Kaoru Okakura , Koichiro Murashima
发明人： Kaoru Okakura , Koichiro Murashima
IPC分类号： C12P19/00 , C12N9/42 , C12P19/14 , C12P19/02 , D06M16/00
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14 , C12Y302/01004 , D06M16/003
摘要： By having a cellulase preparation comprising at least a certain amount of endoglucanases derived from two different types of microorganisms, the cellulase preparation can be provided with a higher activity and a wider pH property than those of cellulase preparations each containing one of the endoglucanases alone. Moreover, by introducing and expressing simultaneously two different types of cellulase genes in a single host cell, a cellulase preparation having a high activity and a wide pH property can be produced easily.
摘要翻译：通过使含有至少一定量的衍生自两种不同类型的微生物的内切葡聚糖酶的纤维素酶制剂，纤维素酶制剂可以提供比单独含有一种内切葡聚糖酶的纤维素酶制剂更高的活性和更宽的pH特性。此外，通过在单个宿主细胞中同时引入和表达两种不同类型的纤维素酶基因，可以容易地制备具有高活性和宽pH特性的纤维素酶制剂。

8. 发明申请

US20100330646A1 THERMOSTABLE CATALASE 有权
标题翻译：热稳定催化剂
公开(公告)号：US20100330646A1
公开(公告)日：2010-12-30
申请号：US12918017
申请日：2009-02-18
申请人： Kaoru Okakura , Fusuke Mazuka , Takayoshi Fukushima , Koichiro Murashima
发明人： Kaoru Okakura , Fusuke Mazuka , Takayoshi Fukushima , Koichiro Murashima
IPC分类号： C12N9/08 , C07H21/04 , C12N15/80 , C12N1/15
CPC分类号： C12N9/0065 , C12Y111/01006
摘要： An object is to efficiently produce thermostable catalase at low cost by expressing it as a recombinant protein in large quantity.A recombinant microorganism capable of efficiently expressing thermostable catalase can be provided by obtaining a DNA necessary for efficiently producing it as a recombinant protein, and the thermostable catalase can be efficiently produced at low cost by cultivating the obtained recombinant microorganism. Hydrogen peroxide can be efficiently decomposed at low cost, even at high temperature, by treating a solution containing hydrogen peroxide with the thermostable catalase of the present invention.
摘要翻译：目的是通过将其大量表达为重组蛋白，以低成本有效地产生热稳定的过氧化氢酶。可以通过获得作为重组蛋白质有效生产所必需的DNA来提供能够有效表达耐热性过氧化氢酶的重组微生物，通过培养所获得的重组微生物，可以低成本有效地制备热稳定性过氧化氢酶。通过用本发明的耐热性过氧化氢酶处理含有过氧化氢的溶液，即使在高温下也能以低成本有效地分解过氧化氢。

9. 发明授权

US07285404B1 Cyclic depsipeptide synthetase and method for recombinant production 失效
标题翻译：循环depipipeptide合成酶和重组生产方法
公开(公告)号：US07285404B1
公开(公告)日：2007-10-23
申请号：US10070387
申请日：2000-09-07
申请人： Naoki Midoh , Kaoru Okakura , Koichi Miyamoto , Manabu Watanabe , Koji Yanai , Tetsuya Yasutake , Sato Aihara , Takafumi Futamura , Horst Kleinkauf , Takeshi Murakami
发明人： Naoki Midoh , Kaoru Okakura , Koichi Miyamoto , Manabu Watanabe , Koji Yanai , Tetsuya Yasutake , Sato Aihara , Takafumi Futamura , Horst Kleinkauf , Takeshi Murakami
IPC分类号： C12N9/00
CPC分类号： C12N9/00 , C12P21/00
摘要： The present invention provides an enzyme that synthesizes a cyclic depsipeptide, particularly the substance 1022, and a gene thereof. A cyclic depsipeptide synthetase according to the present invention comprises (a) an amino acid sequence of SEQ ID NO: 2 or (b) a modified amino acid sequence of the amino acid sequence of SEQ ID NO: 2 that have one or more modifications selected from a substitution, a deletion, an addition and an insertion and has cyclic depsipeptide synthetase activity. A cyclic depsipeptide synthetase gene according to the present invention comprises a nucleotide sequence encoding a cyclic depsipeptide synthetase. The present invention also provides a recombinant vector and a transformant for expressing the cyclic depsipeptide synthetase, and a mass production system for the cyclic depsipeptide. The present invention further provides a method for producing the cyclic depsipeptide synthetase.
摘要翻译：本发明提供合成环状缩肽，特别是物质1022及其基因的酶。根据本发明的循环倍肽合成酶包含（a）SEQ ID NO：2的氨基酸序列或（b）SEQ ID NO：2的氨基酸序列的修饰的氨基酸序列，其具有一个或多个修饰选择从取代，缺失，添加和插入，并具有循环衰老肽合成酶活性。根据本发明的环状缩肽酶合成酶基因包含编码环状倍肽合成酶的核苷酸序列。本发明还提供了用于表达环状缩酚酸肽合成酶的重组载体和转化体，以及用于环状缩酚酸肽的批量生产系统。本发明还提供了一种用于制备环状缩酚酸肽合成酶的方法。

10. 发明授权

US07109018B1 Transformant producing secondary metabolite modified with functional group and novel biosynthesis genes 失效
标题翻译：用功能组和新生物合成基因修饰的转化体产生次级代谢产物
公开(公告)号：US07109018B1
公开(公告)日：2006-09-19
申请号：US10089514
申请日：2000-09-29
申请人： Koji Yanai , Kaoru Okakura , Shohei Yasuda , Manabu Watanabe , Koichi Miyamoto , Naoki Midoh , Takeshi Murakami
发明人： Koji Yanai , Kaoru Okakura , Shohei Yasuda , Manabu Watanabe , Koichi Miyamoto , Naoki Midoh , Takeshi Murakami
IPC分类号： C12N1/20 , C12N5/10 , C07H21/04
CPC分类号： C12N9/0004 , C12N9/88 , C12N9/90 , C12N15/52 , C12P13/001 , C12P13/02
摘要： An objective of the present invention is to provide a transformant altered so as to produce a secondary metabolite in which a benzene ring of the secondary product is modified at the para-position with a functional group containing a nitrogen atom. A transformant according to the present invention is a transformant of an organism producing a secondary metabolite having a benzene ring skeleton without substitution at the para-position with a functional group containing a nitrogen atom, said transformant being transformed by introducing genes involved in a biosynthetic pathway from chorismic acid to p-aminophenylpyruvic acid, including a gene encoding an amino acid sequence (SEQ ID NO: 2) having 4-amino-4-deoxychorismic acid synthase activity, a gene encoding an amino acid sequence (SEQ ID NO: 4) having 4-amino-4-deoxychorismic acid mutase activity and a gene encoding an amino acid sequence (SEQ ID NO: 6) having 4-amino-4-deoxyprephenic acid dehydrogenase activity, so as to produce a secondary metabolite having a benzene ring skeleton substituted at the para-position with a functional group containing a nitrogen atom. Another objective of the present invention is to provide a novel gene involved in the biosynthetic pathway from chorismic acid to p-aminophenylpyruvic acid. A novel gene according to the present invention comprises genes encoding the amino acid sequences of SEQ ID NOs: 2, 4 and 6 or modified sequences thereof.
摘要翻译：本发明的目的是提供改性的转化体，以便产生次级代谢物，其中次级产物的苯环在对位上用含氮原子的官能团改性。根据本发明的转化体是产生具有苯环骨架的次级代谢物的生物体的转化体，其在具有含氮原子的官能团的对位没有取代，所述转化体通过引入参与生物合成途径的基因而转化包括编码氨基酸序列（SEQ ID NO：4）的氨基酸序列（SEQ ID NO：2）的编码氨基酸序列（SEQ ID NO：4）的氨基酸序列（SEQ ID NO：2）的基因的氨基苯基丙酮酸，具有4-氨基-4-脱氧酸性变位酶活性的基因，以及编码具有4-氨基-4-脱氧蝶呤酸脱氢酶活性的氨基酸序列（SEQ ID NO：6）的基因，以产生具有苯环骨架的次级代谢物在对位上用含有氮原子的官能团取代。本发明的另一个目的是提供一种涉及从氯酸到对氨基苯基丙酮酸生物合成途径的新基因。根据本发明的新基因包含编码SEQ ID NO：2,4和6的氨基酸序列的基因或其修饰序列。

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