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1. 发明授权

US08552170B2 Expression vector for establishing highly productive cell and the highly productive cell 失效
标题翻译：用于建立高产细胞和高产细胞的表达载体
公开(公告)号：US08552170B2
公开(公告)日：2013-10-08
申请号：US13122119
申请日：2010-06-04
申请人： Tomomi Yamazaki , Kenji Masuda , Shigeaki Nishii , Bunsei Kawakami
发明人： Tomomi Yamazaki , Kenji Masuda , Shigeaki Nishii , Bunsei Kawakami
IPC分类号： C07H21/04 , C12N15/85 , C12P21/00
CPC分类号： C07K16/1203 , C07K2317/14 , C12N15/67 , C12N15/85 , C12N2830/50
摘要： The present invention provides an expression vector which is effective in an efficient establishment of transformed cells which express the aimed protein gene in a high level. An expression vector which has a cassette for expressing the drug selective marker gene containing mRNA destabilizing sequence, at least one element for stabilizing the gene expression and a cassette for expressing the gene of the aimed protein. Preferably, the mRNA destabilizing sequence is derived from AT-rich sequence existing in the 3′-untranslated region of cytokine, interleukin or proto-oncogene, and the element for stabilizing the gene expression is derived from Chinese hamster genome.
摘要翻译：本发明提供了一种有效建立高水平表达目的蛋白质基因的转化细胞的表达载体。具有用于表达含有mRNA稳定化序列的药物选择标记基因的盒的表达载体，用于稳定基因表达的至少一种元件和用于表达目标蛋白质的基因的盒。优选地，mRNA去稳定化序列衍生自存在于细胞因子，白细胞介素或原癌基因的3'非翻译区的富含AT的序列，并且用于稳定基因表达的元件来源于中国仓鼠基因组。

2. 发明申请

US20110281286A1 EXPRESSION VECTOR FOR ESTABLISHING HIGHLY PRODUCTIVE CELL AND THE HIGHLY PRODUCTIVE CELL 失效
标题翻译：用于建立高产率细胞和高产量细胞的表达载体
公开(公告)号：US20110281286A1
公开(公告)日：2011-11-17
申请号：US13122119
申请日：2010-06-04
申请人： Tomomi Yamazaki , Kenji Masuda , Shigeaki Nishii , Bunsei Kawakami
发明人： Tomomi Yamazaki , Kenji Masuda , Shigeaki Nishii , Bunsei Kawakami
IPC分类号： C12N15/85 , C12Q1/66 , C12N5/10 , C12Q1/02 , C12P21/00
CPC分类号： C07K16/1203 , C07K2317/14 , C12N15/67 , C12N15/85 , C12N2830/50
摘要： The present invention provides an expression vector which is effective in an efficient establishment of transformed cells which express the aimed protein gene in a high level. An expression vector which has a cassette for expressing the drug selective marker gene containing mRNA destabilizing sequence, at least one element for stabilizing the gene expression and a cassette for expressing the gene of the aimed protein. Preferably, the mRNA destabilizing sequence is derived from AT-rich sequence existing in the 3′-untranslated region of cytokine, interleukin or proto-oncogene, and the element for stabilizing the gene expression is derived from Chinese hamster genome.
摘要翻译：本发明提供了一种有效建立高水平表达目的蛋白质基因的转化细胞的表达载体。具有用于表达含有mRNA稳定化序列的药物选择性标记基因的盒的表达载体，用于稳定基因表达的至少一种元件和用于表达目标蛋白质的基因的盒。优选地，mRNA去稳定化序列衍生自存在于细胞因子，白细胞介素或原癌基因的3'非翻译区的富含AT的序列，并且用于稳定基因表达的元件来源于中国仓鼠基因组。

3. 发明授权

US08470974B2 Method for highly sensitive detection of protein-protein interaction 有权
标题翻译：高灵敏度检测蛋白质 - 蛋白质相互作用的方法
公开(公告)号：US08470974B2
公开(公告)日：2013-06-25
申请号：US13375142
申请日：2010-05-28
申请人： Takeaki Ozawa , Naomi Misawa , Kenji Miura , Tasuku Okamoto , Shigeaki Nishii , Kenji Masuda
发明人： Takeaki Ozawa , Naomi Misawa , Kenji Miura , Tasuku Okamoto , Shigeaki Nishii , Kenji Masuda
IPC分类号： C07K1/00 , C12Q1/00 , G01N33/53 , C12P21/04 , A61K39/02
CPC分类号： C12Q1/6804 , C12N9/0069 , C12Q1/66 , G01N33/542
摘要： The present invention intends to provide an assay system using split luciferase that has a remarkably high detection sensitivity. In an embodiment, binding of mutually binding first and second proteins is detected by preparing a first fusion protein comprising the first protein fused with a peptide having the amino acid sequence of amino acid SEQ ID NO: 1 and a second fusion protein comprising the second protein fused with a peptide having an amino acid sequence selected from the group consisting of amino acid SEQ ID NOS: 2 to 6, and allowing the first fusion protein to bind with the second fusion protein to form a complex, and detecting luminescence emitted from the complex.
摘要翻译：本发明意图提供一种使用分辨荧光素酶的测定系统，其具有非常高的检测灵敏度。在一个实施方案中，通过制备包含与具有氨基酸SEQ ID NO：1的氨基酸序列的肽融合的第一蛋白质的第一融合蛋白和包含第二蛋白质的第二融合蛋白来检测相互结合的第一和第二蛋白质的结合与具有选自氨基酸SEQ ID NO：2至6的氨基酸序列的肽融合，并使第一融合蛋白与第二融合蛋白结合形成复合物，并检测从复合物发射的发光。

4. 发明申请

US20120190824A1 METHOD FOR HIGHLY SENSITIVE DETECTION OF PROTEIN-PROTEIN INTERACTION 有权
标题翻译：用于敏感性检测蛋白质 - 蛋白质相互作用的方法
公开(公告)号：US20120190824A1
公开(公告)日：2012-07-26
申请号：US13375142
申请日：2010-05-28
申请人： Takeaki Ozawa , Naomi Misawa , Kenji Miura , Tasuku Okamoto , Shigeaki Nishii , Kenji Masuda
发明人： Takeaki Ozawa , Naomi Misawa , Kenji Miura , Tasuku Okamoto , Shigeaki Nishii , Kenji Masuda
IPC分类号： C07K14/435
CPC分类号： C12Q1/6804 , C12N9/0069 , C12Q1/66 , G01N33/542
摘要： The present invention intends to provide an assay system using split luciferase that has a remarkably high detection sensitivity. In an embodiment, binding of mutually binding first and second proteins is detected by preparing a first fusion protein comprising the first protein fused with a peptide having the amino acid sequence of amino acid SEQ ID NO: 1 and a second fusion protein comprising the second protein fused with a peptide having an amino acid sequence selected from the group consisting of amino acid SEQ ID NOS: 2 to 6, and allowing the first fusion protein to bind with the second fusion protein to form a complex, and detecting luminescence emitted from the complex.
摘要翻译：本发明意图提供一种使用分辨荧光素酶的测定系统，其具有非常高的检测灵敏度。在一个实施方案中，通过制备包含与具有氨基酸SEQ ID NO：1的氨基酸序列的肽融合的第一蛋白质的第一融合蛋白和包含第二蛋白质的第二融合蛋白来检测相互结合的第一和第二蛋白质的结合与具有选自氨基酸SEQ ID NO：2至6的氨基酸序列的肽融合，并使第一融合蛋白与第二融合蛋白结合形成复合物，并检测从复合物发射的发光。

5. 发明授权

US08784040B2 Ventilator 有权
标题翻译：呼吸机
公开(公告)号：US08784040B2
公开(公告)日：2014-07-22
申请号：US12919335
申请日：2008-05-26
申请人： Kenji Masuda , Yoshikatsu Nankaku , Daisuke Nagasaki , Akihiro Ikawa
发明人： Kenji Masuda , Yoshikatsu Nankaku , Daisuke Nagasaki , Akihiro Ikawa
IPC分类号： F01D15/00 , F04D29/60 , F24F7/013 , F04D25/12 , F04D29/42
CPC分类号： F24F7/007 , F04D25/12 , F04D29/4226 , F04D29/601 , F24F7/013
摘要： A ventilator includes a body casing having an opening formed in its lower portion and an exhaust port formed on one side; a fan that is provided in the body casing, for sucking indoor air from the opening and exhausting the indoor air from the exhaust port; a resin fan casing that has a suction port and forms an air passage of the fan; a protection frame formed outside the air passage to cover a terminal block provided outside the air passage; a resin terminal block cover that watertightly seals a protection frame opening of a lower portion of the protection frame and is opened and closed; a sheet-metal case that is provided in the protection frame and stores the terminal block; and a sheet-metal terminal block cover that opens and closes a lower opening of the sheet-metal case.
摘要翻译：一种通风机，包括：主体壳体，其具有形成在其下部的开口和形成在一侧的排气口; 风扇，设置在所述主体壳体中，用于从所述开口吸入室内空气并从所述排气口排出室内空气; 树脂风扇壳体，其具有吸入口并形成风扇的空气通道; 保护框架，其形成在所述空气通道的外侧，以覆盖设置在所述空气通道外部的端子块; 树脂端子块盖，其将保护框架的下部的保护框架开口水密地密封并打开和关闭; 设置在保护框架中并存储端子块的金属片外壳; 以及打开和关闭金属薄板壳体的下部开口的金属板端子块盖。

6. 发明申请

US20090326136A1 PROPYLENE-BASED BLOCK COPOLYMER COMPOSITION AND EXTERIOR MEMBER FOR AUTOMOBILE 审中-公开
标题翻译：丙烯基嵌段共聚物组合物和汽车外部成员
公开(公告)号：US20090326136A1
公开(公告)日：2009-12-31
申请号：US12304584
申请日：2007-06-15
申请人： Kenji Masuda , Ken Shimizu , Minoru Adachi , Hiroshi Inanami , Kaoru Inoue
发明人： Kenji Masuda , Ken Shimizu , Minoru Adachi , Hiroshi Inanami , Kaoru Inoue
IPC分类号： C08K3/34
CPC分类号： C08L53/00 , C08K3/34 , C08K9/04 , C08L23/0815 , C08L2666/06 , C08L2666/08 , C08L2666/04
摘要： A polypropylene resin composition for molding material, which is excellent in an appearance of weld line and an appearance of tiger stripe and is used for automobile exterior parts and an automobile exterior part comprising the same, are provided. A propylene-based block copolymer composition, comprising 100 parts by weight of a propylene-based block copolymer having an MFR of 50 to 100 g/10 minutes and an Mw/Mn of 7 or less, wherein the propylene-based block copolymer comprises 75 to 95% by weight of a crystalline polypropylene portion and 5 to 25% by weight of an ethylene-propylene copolymer portion, where the ethylene content of the ethylene-propylene copolymer portion is 35 to 45% by weight and the ratio of the weight-average molecular weight of the ethylene-propylene copolymer portion to the weight-average molecular weight of the crystalline polypropylene portion is 3 to 5; 25 to 45 parts by weight of an ethylene-α-olefin copolymer elastomer having an MFR of 1 to 9 g/10 minutes; and 30 to 45 parts by weight of talc having an average particle diameter of 1.5 to 15 μm.
摘要翻译：提供了一种用于成型材料的聚丙烯树脂组合物，其外观为焊接线的外观和虎纹的外观，并且用于汽车外部部件和包括该材料的汽车外部部件。一种基于丙烯的嵌段共聚物组合物，其包含100重量份MFR为50至100g / 10分钟和Mw / Mn为7或更小的丙烯基嵌段共聚物，其中所述丙烯类嵌段共聚物包含75 至95重量％的结晶聚丙烯部分和5至25重量％的乙烯 - 丙烯共聚物部分，其中乙烯 - 丙烯共聚物部分的乙烯含量为35-45重量％，并且重量 - 乙烯 - 丙烯共聚物部分的平均分子量与结晶聚丙烯部分的重均分子量为3〜5; 25〜45重量份MFR为1〜9g / 10分钟的乙烯-α-烯烃共聚物弹性体; 和30〜45重量份的平均粒径为1.5〜15μm的滑石。

7. 发明申请

US20050142236A1 Composition comprising an aqueous extract of red vine leaves and a diuretic 审中-公开
标题翻译：组合物，其包含红藤叶的水提取物和利尿剂
公开(公告)号：US20050142236A1
公开(公告)日：2005-06-30
申请号：US11002054
申请日：2004-12-02
申请人： Toshiaki Horie , Kenji Masuda , Minoru Okada , Koichi Takahashi
发明人： Toshiaki Horie , Kenji Masuda , Minoru Okada , Koichi Takahashi
IPC分类号： A61K31/4415 , A61K31/522 , A61K36/00 , A61K36/481 , A61K36/725 , A61K36/87 , A61K45/06 , A61P9/10 , A61P9/14 , A61K35/78
CPC分类号： A61K45/06 , A61K31/4415 , A61K31/522 , A61K36/481 , A61K36/725 , A61K36/87 , A61K2300/00
摘要： This invention relates to a new composition containing the effective dosage of an aqueous extract of red vine leaves (1) and a diuretic (2) for preventing or alleviating the discomfort associated with mild-to-moderate chronic venous insufficiency of the legs. The compositions according to this invention may also contain pharmaceutically or dietetically acceptable additives.
摘要翻译：本发明涉及一种新的组合物，其含有有效剂量的红藤叶提取物（1）和利尿剂（2），用于预防或减轻与轻度至中度的慢性静脉功能不全相关的不适。根据本发明的组合物还可以含有药学上或饮食上可接受的添加剂。

8. 发明授权

US5858800A Optical measurement method and apparatus thereof 失效
标题翻译：光学测量方法及其装置
公开(公告)号：US5858800A
公开(公告)日：1999-01-12
申请号：US586876
申请日：1996-03-22
申请人： Kazuhisa Shigemori , Kenji Masuda
发明人： Kazuhisa Shigemori , Kenji Masuda
IPC分类号： G01N21/27 , G01N21/64 , G01N33/534 , G01N33/552
CPC分类号： G01N21/648 , G01N21/6428 , Y10S435/808 , Y10S436/805
摘要： An exciting light 2 is introduced within a slab-type optical waveguide 1, an evanescent wave due to the exciting light 2 excites fluorescent light, and among the fluorescent light, a fluorescent light which is radiated in a direction which direction crosses the exciting light 2 by a predetermined angle, is detected by a fluorescent light detector 4. Consequently, the exciting light and the fluorescent light are spacially separated so as to reduce stray light, and an arrangement of an optical system is simplified.
摘要翻译： PCT No.PCT / JP95 / 01010 Sec。 371日期：1996年3月22日 102（e）1996年3月22日PCT PCT 1995年5月25日PCT公布。出版物WO95 / 32417 日期：1995年11月30日在平板型光波导1内引入激励光2，由于激发光2引起的ev逝波激发荧光，在荧光中，沿着方向通过荧光检测器4检测激发光2与预定角度相交。因此，激发光和荧光被空间分离，以便减少杂散光，并简化了光学系统的布置。

9. 发明授权

US08711152B2 Animation control apparatus, animation control method, and non-transitory computer readable recording medium 失效
标题翻译：动画控制装置，动画控制方法以及非暂时性计算机可读记录介质
公开(公告)号：US08711152B2
公开(公告)日：2014-04-29
申请号：US13390604
申请日：2011-05-31
申请人： Kenji Masuda , Mitsuhiro Aso
发明人： Kenji Masuda , Mitsuhiro Aso
IPC分类号： G06T13/00 , G06T13/80
CPC分类号： G06T13/80 , G06T13/00
摘要： An animation control apparatus includes an interpolation component information creating unit that interpolates first and second component information included in first and second keyframe information, to create interpolation component information expressing an interpolation screen component; a rendering time period computing unit that calculates a rendering time period required in a rendering process of the interpolation screen component; and a rendering determination unit that determines, based on the rendering time period, whether or not the rendering process of the interpolation screen component is completed by a second rendering start time included in the second keyframe information. A display controller waits without performing the rendering process of the interpolation screen component, when determination is made that the rendering process is not completed by the second rendering start time, and then starts a rendering process of a screen component expressed by the second component information.
摘要翻译：动画控制装置包括内插分量信息生成部，对包含在第1关键帧信息和第2关键帧信息中的第1分量信息和第2分量信息进行内插，生成表示内插画面成分的内容分量信息; 渲染时间计算单元，其计算所述插值屏幕分量的呈现处理所需的渲染时间段; 以及渲染确定单元，其基于所述呈现时间段，确定所述插值屏幕组件的呈现处理是否通过包括在所述第二关键帧信息中的第二渲染开始时间来完成。当确定在第二渲染开始时间没有完成呈现处理，然后开始由第二分量信息表示的屏幕分量的渲染处理时，显示控制器等待而不执行插值屏幕分量的渲染处理。

10. 发明授权

US06801553B2 Wavelength monitor and semiconductor laser device 失效
标题翻译：波长监视器和半导体激光器件
公开(公告)号：US06801553B2
公开(公告)日：2004-10-05
申请号：US09988662
申请日：2001-11-20
申请人： Masao Imaki , Yoshihito Hirano , Makoto Sato , Kenji Masuda , Akihiro Adachi , Yasunori Nishimura , Shinichi Takagi
发明人： Masao Imaki , Yoshihito Hirano , Makoto Sato , Kenji Masuda , Akihiro Adachi , Yasunori Nishimura , Shinichi Takagi
IPC分类号： H01S313
CPC分类号： H01S5/0687 , H01S5/005 , H01S5/02248
摘要： A wavelength monitor comprises a cylindrical lens configured to allow a laser beam emitted from a semiconductor laser to pass therethrough, first and second photodetectors configured to receive the laser beam through the cylindrical lens, and a wavelength filter disposed in an optical path between the semiconductor laser and the first photodetector.
摘要翻译：波长监视器包括被配置为允许从半导体激光器发射的激光束通过的柱面透镜，被配置为接收通过柱面透镜的激光束的第一和第二光电检测器，以及设置在半导体激光器之间的光路中的波长滤光器和第一光电检测器。

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