专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US5871904A Immunassay of non-A, non-B hepatitis virus-related antigens, monoclonal antibodies for use therein, and hybridomas producing the antibodies 失效
标题翻译：非A型非乙型肝炎病毒相关抗原的免疫测定，用于其中的单克隆抗体和产生抗体的杂交瘤
公开(公告)号：US5871904A
公开(公告)日：1999-02-16
申请号：US501195
申请日：1995-07-11
申请人： Tomiko Kashiwakuma , Shintaro Yagi , Akira Hasegawa , Tadahiro Kajita , Yohsuke Ohta , Hiroyuki Mori
发明人： Tomiko Kashiwakuma , Shintaro Yagi , Akira Hasegawa , Tadahiro Kajita , Yohsuke Ohta , Hiroyuki Mori
IPC分类号： G01N33/53 , C07K14/18 , C07K16/10 , C12N5/10 , C12N5/20 , C12P21/08 , G01N33/576 , G01N33/577 , C12Q1/70 , C07K16/08 , C12N5/12
CPC分类号： C07K14/005 , C07K16/10 , C12N2770/24222
摘要： This invention concerns a monoclonal antibody having binding specificity for an antigenic determinant site on core structural protein from Non-A,Non-B hepatitis virus (NANBV); a hybridoma cell line capable of producing the monoclonal antibody; a process for the preparation of the monoclonal antibody; an immunoassay of NANBV-related antigens by use of the monoclonal antibody; and a test kit for use in the immunoassay. The preferred monoclonal antibody is 5E3, 5F11, 515S or 1080S. The monoclonal antibody can specifically recognize the NANBV core structural protein in sera from patients with Non-A,Non-B hepatitis thereby being served extensively as an antibody in various immunological reagents for definitive diagnosis of Non-A, Non-B hepatitis.
摘要翻译：本发明涉及对来自非A非乙型肝炎病毒（NANBV）的核心结构蛋白上的抗原决定簇位点具有结合特异性的单克隆抗体; 能够产生单克隆抗体的杂交瘤细胞系; 一种制备单克隆抗体的方法; 通过使用单克隆抗体对NANBV相关抗原的免疫测定; 和用于免疫测定的测试试剂盒。优选的单克隆抗体是5E3,5F11,515S或1080S。单克隆抗体可以特异性识别来自非A型非乙型肝炎患者的血清中的NANBV核心结构蛋白，从而作为用于非A型非乙型肝炎确定诊断的各种免疫试剂中的抗体作为抗体。

2. 发明授权

US5800982A Antigenic peptides for growing hepatitis C virus, kit comprising the same and methods for its grouping using the same 失效
标题翻译：用于生长丙型肝炎病毒的抗原肽，包含其的试剂盒以及使用其组合的方法
公开(公告)号：US5800982A
公开(公告)日：1998-09-01
申请号：US685764
申请日：1996-07-24
申请人： Akira Hasegawa , Noboru Maki , Shintaro Yagi , Tomiko Kashiwakuma , Kenjiro Yamaguchi , Naoko Ikeguchi , Tomoko Kobayashi , Chiaki Senoo
发明人： Akira Hasegawa , Noboru Maki , Shintaro Yagi , Tomiko Kashiwakuma , Kenjiro Yamaguchi , Naoko Ikeguchi , Tomoko Kobayashi , Chiaki Senoo
IPC分类号： C07K14/18 , C12N15/51 , C12Q1/70 , A61K39/29
CPC分类号： C07K14/005 , C12N2770/24222
摘要： The present invention relates to an NS4-related antigenic peptide capable of reacting specifically with antibodies directed against Group I of hepatitis C virus (HCV); to an NS4-related antigenic peptide capable of reacting specifically with antibodies directed against Group II of HCV; to a kit for identifying HCV Groups I or II which comprises said peptides in separate sections; and to methods for grouping HCV. The peptides of the present invention can be used to detect HCV Groups I or II specifically as well as the detection of patients having the mixed infection, by which HCV Group II-infected patients can receive early interferon treatment effectively. The peptides are also useful for diagnosis of HCV infection.
摘要翻译：本发明涉及能够特异性地针对丙型肝炎病毒（HCV）I组抗体的NS4相关抗原肽; 涉及能够与针对HCV II组的抗体特异性反应的NS4相关抗原肽; 用于鉴定HCV组I或II的试剂盒，其包含分开部分中的所述肽; 以及HCV分组方法。本发明的肽可用于特异性检测HCV组I或II，以及检测具有混合感染的患者，由此可以有效地接受HCV II类感染患者的早期干扰素治疗。这些肽也可用于诊断HCV感染。

3. 发明授权

US06322965B1 Chimera antigen peptide 失效
标题翻译：嵌合抗原肽
公开(公告)号：US06322965B1
公开(公告)日：2001-11-27
申请号：US09020846
申请日：1998-02-09
申请人： Kenjiro Yamaguchi , Tomiko Kashiwakuma , Yukie Chiba , Shintaro Yagi , Akira Hasegawa
发明人： Kenjiro Yamaguchi , Tomiko Kashiwakuma , Yukie Chiba , Shintaro Yagi , Akira Hasegawa
IPC分类号： C12Q168
CPC分类号： C07K14/005 , A61K38/00 , C07K2319/00 , C12N2770/24222 , Y10S436/811 , Y10S436/82 , Y10S530/806 , Y10S530/826
摘要： A chimera antigen peptide containing the epitope regions in the core region, the NS3 region, and the NS4 region of the HCV polypeptide. A sensitive detection of a wide range of infection by hepatitis C virus (HCV) can be carried out.
摘要翻译：含有HCV多核苷酸核心区域，NS3区域和NS4区域中的表位区域的嵌合抗原肽。可以进行丙型肝炎病毒（HCV）的广泛感染的敏感检测。

4. 发明申请

US20100120036A1 METHOD FOR AMPLIFYING DNA FRAGMENT 审中-公开
标题翻译：放大DNA片段的方法
公开(公告)号：US20100120036A1
公开(公告)日：2010-05-13
申请号：US12530033
申请日：2008-03-05
申请人： Kunio Shiota , Shintaro Yagi , Yoko Takahashi , Satoshi Tanaka , Jun Ohgane
发明人： Kunio Shiota , Shintaro Yagi , Yoko Takahashi , Satoshi Tanaka , Jun Ohgane
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/683 , C12Q2531/113 , C12Q2525/155 , C12Q2521/331
摘要： To measure the methylation degree of a genomic DNA simply and correctly as well as exhaustively, there is provided a method for amplifying a DNA fragment, characterized by comprising the following steps (1) to (5): (1) digesting a sample double-stranded DNA with a first restriction enzyme; (2) adding a double-stranded DNA fragment consisting of a first oligonucleotide and a second oligonucleotide to a restriction enzyme cleavage end of the double-stranded DNA fragment produced in step (1) to obtain a DNA fragment; (3) digesting the DNA fragment obtained in step (2) with a second restriction enzyme; (4) allowing the double-stranded DNA fragment produced in step (3) to coexist with a third oligonucleotide and a fourth oligonucleotide in the presence of a ligase; and (5) heating the double-stranded DNA fragment obtained in step (4), thereby dissociating the double-stranded DNA fragment and then performing a polymerase chain reaction to selectively amplify a DNA fragment having the cleavage end of the first restriction enzyme and the cleavage end of the second restriction enzyme.
摘要翻译：为了简单正确地，全面地测定基因组DNA的甲基化程度，提供了扩增DNA片段的方法，其特征在于包括以下步骤（1）〜（5）：（1）用第一限制酶的双链DNA; （2）将由第一寡核苷酸和第二寡核苷酸组成的双链DNA片段加入步骤（1）中制备的双链DNA片段的限制酶切割末端以获得DNA片段; （3）用第二限制酶消化步骤（2）中获得的DNA片段; （4）在连接酶存在下使步骤（3）中产生的双链DNA片段与第三寡核苷酸和第四寡核苷酸共存; （5）加热步骤（4）中获得的双链DNA片段，从而解离双链DNA片段，然后进行聚合酶链式反应以选择性扩增具有第一限制酶切割末端的DNA片段，第二限制酶的切割末端。

5. 发明授权

US07776542B1 Methods for the detection of hepatitis B and C viruses 有权
标题翻译：检测乙型和丙型肝炎病毒的方法
公开(公告)号：US07776542B1
公开(公告)日：2010-08-17
申请号：US09269897
申请日：1998-08-04
申请人： Katsumi Aoyagi , Chiharu Ohue , Kumiko Iida , Tatsuji Kimura , Shintaro Yagi
发明人： Katsumi Aoyagi , Chiharu Ohue , Kumiko Iida , Tatsuji Kimura , Shintaro Yagi
IPC分类号： G01N33/53 , G01N33/569 , G01N33/576
CPC分类号： G01N33/5767 , G01N33/56983 , G01N2333/02 , G01N2333/18
摘要： A method for treating a virus-containing sample, characterized by treatment of a virus-containing sample with a treatment solution containing (1) an anionic surfactant and (2) an amphoteric surfactant, nonionic surfactant or protein denaturant; a virus assay method using said treating method; a method for treating a virus-containing sample, characterized by treatment of a virus-containing sample with a treatment solution containing (1) a chaotropic ion and (2) an acidifying agent; a virus assay method using said treating method; a virus assay method, characterized in that a virus antigen and a virus antibody are measured based on their binding to their probe in the presence of a surfactant with an alkyl group of 10 or more carbon atoms and a secondary, tertiary or quaternary amine, or a nonionic surfactant, or of both of them; and a monoclonal antibody and a hybridoma producing the same for carrying out said method.
摘要翻译：一种治疗含病毒样品的方法，其特征在于用含有（1）阴离子表面活性剂和（2）两性表面活性剂，非离子表面活性剂或蛋白质变性剂的处理溶液处理病毒样品; 使用所述处理方法的病毒测定方法; 一种用于处理含病毒样品的方法，其特征在于用含有（1）离液序列离子的处理溶液和（2）酸化剂处理含有病毒的样品; 使用所述处理方法的病毒测定方法; 一种病毒测定方法，其特征在于，在具有10个或更多个碳原子的烷基和二级，三级或季胺的表面活性剂存在下，基于它们与它们的探针的结合来测量病毒抗原和病毒抗体，或非离子表面活性剂，或它们两者; 以及用于实施所述方法的单克隆抗体和产生其的杂交瘤。

6. 发明授权

US07323331B2 Method for detecting or assaying HBV 有权
标题翻译： HBV检测方法
公开(公告)号：US07323331B2
公开(公告)日：2008-01-29
申请号：US10343324
申请日：2001-08-10
申请人： Noboru Maki , Tatsuji Kimura , Yoko Oda , Shintaro Yagi
发明人： Noboru Maki , Tatsuji Kimura , Yoko Oda , Shintaro Yagi
IPC分类号： C12N5/00 , C12N5/06 , C12N5/12 , C12N5/18 , C12N5/20 , C12Q1/00 , C12Q1/70 , G01N33/53 , C07K16/08
CPC分类号： G01N33/5762 , C07K16/082
摘要： A Method for detecting hepatitis B virus as well as a kit and a reagent therefor, comprising using a phosphate buffer that specifically binds to hepatitis B virus, for example a monoclonal antibody that specifically binds to a specific site of core-related protein of hepatitis B virus.
摘要翻译：一种检测乙型肝炎病毒的方法以及试剂盒及其试剂，其包括使用特异性结合乙型肝炎病毒的磷酸盐缓冲液，例如特异性结合乙型肝炎核心相关蛋白质的特定位点的单克隆抗体病毒。

7. 发明授权

US06623921B2 Method for measurement of hepatitis C virus 有权
公开(公告)号：US06623921B2
公开(公告)日：2003-09-23
申请号：US10133007
申请日：2002-04-26
申请人： Katsumi Aoyagi , Chiharu Ohue , Kumiko Iida , Shintaro Yagi
发明人： Katsumi Aoyagi , Chiharu Ohue , Kumiko Iida , Shintaro Yagi
IPC分类号： C12Q170
CPC分类号： G01N33/5767 , G01N2333/18 , G01N2469/10 , G01N2469/20 , Y10S435/962 , Y10T436/108331
摘要： A method for measurement of the hepatitis C virus (HCV) characterized by measuring HCV core antigen and HCV core antibody by their binding with probes in the presence of an anionic surfactant or a non-ionic surfactant, or both.

8. 发明授权

US08383333B2 HBV precore protein capable of forming particles 有权
标题翻译： HBV前蛋白能够形成颗粒
公开(公告)号：US08383333B2
公开(公告)日：2013-02-26
申请号：US12654280
申请日：2009-12-16
申请人： Noboru Maki , Tatsuji Kimura , Shintaro Yagi
发明人： Noboru Maki , Tatsuji Kimura , Shintaro Yagi
IPC分类号： C12Q1/70 , G01N33/53
CPC分类号： C07K14/005 , A61K2039/5258 , C12N7/00 , C12N2730/10122 , C12N2730/10123 , G01N33/5761
摘要： There is provided a HBV precore protein having an ability of forming particles, and a means for determining it. A novel HBV precore protein that forms the virus (like) particles of HBV was identified. The present invention provides this novel HBV precore protein. Furthermore, there are provided core-like particles and virus-like particles formed by this HBV precore protein. These virus-like particles can be used for vaccines and therapeutic agents. The present invention also provides a method of determining the HBV precore protein and a method of determining the anti-HBV precore protein antibody.
摘要翻译：提供了具有形成颗粒的能力的HBV前核蛋白，以及用于确定它的手段。鉴定出形成HBV病毒（如）颗粒的新型HBV前核蛋白。本发明提供了这种新型HBV前核蛋白。此外，还提供了由该HBV前核蛋白形成的核心状颗粒和病毒样颗粒。这些病毒样颗粒可用于疫苗和治疗剂。本发明还提供了确定HBV前核蛋白的方法以及确定抗HBV前蛋白抗体的方法。

9. 发明申请

US20100285444A1 HBV precore protein capable of forming particles 有权
公开(公告)号：US20100285444A1
公开(公告)日：2010-11-11
申请号：US12654280
申请日：2009-12-16
申请人： Noboru Maki , Tatsuji Kimura , Shintaro Yagi
发明人： Noboru Maki , Tatsuji Kimura , Shintaro Yagi
IPC分类号： C12Q1/70
CPC分类号： C07K14/005 , A61K2039/5258 , C12N7/00 , C12N2730/10122 , C12N2730/10123 , G01N33/5761
摘要： There is provided a HBV precore protein having an ability of forming particles, and a means for determining it. A novel HBV precore protein that forms the virus (like) particles of HBV was identified. The present invention provides this novel HBV precore protein. Furthermore, there are provided core-like particles and virus-like particles formed by this HBV precore protein. These virus-like particles can be used for vaccines and therapeutic agents. The present invention also provides a method of determining the HBV precore protein and a method of determining the anti-HBV precore protein antibody.

10. 发明授权

US07316905B1 Method for measurement of hepatitis C virus 有权
标题翻译：丙型肝炎病毒测定方法
公开(公告)号：US07316905B1
公开(公告)日：2008-01-08
申请号：US09509449
申请日：1999-07-30
申请人： Katsumi Aoyagi , Chiharu Ohue , Kumiko Iida , Shintaro Yagi
发明人： Katsumi Aoyagi , Chiharu Ohue , Kumiko Iida , Shintaro Yagi
IPC分类号： G01N33/53 , G01N33/569 , C12Q1/70 , C12Q1/00 , C07K2/00 , C07K14/00 , C07K14/08 , C07K16/00 , C07K16/08 , C07K16/10
CPC分类号： G01N33/5767 , G01N2333/18 , G01N2469/10 , G01N2469/20
摘要： A method for measurement of the hepatitis C virus (HCV) characterized by measuring HCV core antigen and HCV core antibody by their binding with probes in the presence of an anionic surfactant or a non-ionic surfactant, or both.
摘要翻译：一种用于测量丙型肝炎病毒（HCV）的方法，其特征在于通过在阴离子表面活性剂或非离子表面活性剂或两者的存在下与探针结合来测量HCV核心抗原和HCV核心抗体。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式