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1. 发明授权

US08124031B2 Discharge volume control method, discharge pressure control method, and microbody forming method 有权
公开(公告)号：US08124031B2
公开(公告)日：2012-02-28
申请号：US11785431
申请日：2007-04-17
申请人： Tohru Harada , Akihiko Yabuki , Kiyoshi Taninaka
发明人： Tohru Harada , Akihiko Yabuki , Kiyoshi Taninaka
IPC分类号： B01L3/02
CPC分类号： C12M35/00 , Y10T137/0396 , Y10T137/0971 , Y10T137/3115 , Y10T137/85978
摘要： A second valve is opened to combine an injection-pressure generating pressure with a maintaining pressure into an injection pressure. After the injection pressure is applied to a capillary to cause discharge of an object therefrom, an output pressure of a regulator is set to the injection pressure. Then, a first valve is opened to reapply the injection pressure to the capillary. The second valve is opened to combine a maintaining-pressure generating pressure with the injection pressure into the maintaining pressure. After the maintaining pressure is applied to the capillary to terminate the discharge of the object, the output pressure of the regulator is set to the maintaining pressure. Then, the first valve is opened to reapply the maintaining pressure to the capillary.

2. 发明申请

US20080079376A1 Discharge volume control method, discharge pressure control method, injecting apparatus, microbody forming method, discharge volume control device, and computer product 有权
标题翻译：放电量控制方法，排出压力控制方法，注射装置，微体成形方法，排出量控制装置和计算机产品
公开(公告)号：US20080079376A1
公开(公告)日：2008-04-03
申请号：US11785431
申请日：2007-04-17
申请人： Tohru Harada , Akihiko Yabuki , Kiyoshi Taninaka
发明人： Tohru Harada , Akihiko Yabuki , Kiyoshi Taninaka
IPC分类号： H02P7/00
CPC分类号： C12M35/00 , Y10T137/0396 , Y10T137/0971 , Y10T137/3115 , Y10T137/85978
摘要： A second valve is opened to combine an injection-pressure generating pressure with a maintaining pressure into an injection pressure. After the injection pressure is applied to a capillary to cause discharge of an object therefrom, an output pressure of a regulator is set to the injection pressure. Then, a first valve is opened to reapply the injection pressure to the capillary. The second valve is opened to combine a maintaining-pressure generating pressure with the injection pressure into the maintaining pressure. After the maintaining pressure is applied to the capillary to terminate the discharge of the object, the output pressure of the regulator is set to the maintaining pressure. Then, the first valve is opened to reapply the maintaining pressure to the capillary.
摘要翻译：打开第二阀，将注射压力产生压力与保持压力组合成注射压力。在向毛细管施加注射压力以使物体排出之后，将调节器的输出压力设定为注射压力。然后，打开第一阀，以将注射压力重新应用于毛细管。第二阀打开，将维持压力产生压力与喷射压力组合成维持压力。在对毛细管施加维持压力以终止物体的排出之后，将调节器的输出压力设定为保持压力。然后，打开第一个阀，以将保持压力重新应用到毛细管。

3. 发明授权

US08241590B2 Discharge volume control method, discharge pressure control method, and microbody forming method 有权
标题翻译：放电量控制方法，排气压力控制方法和微体成形方法
公开(公告)号：US08241590B2
公开(公告)日：2012-08-14
申请号：US13354808
申请日：2012-01-20
申请人： Tohru Harada , Akihiko Yabuki , Kiyoshi Taninaka
发明人： Tohru Harada , Akihiko Yabuki , Kiyoshi Taninaka
IPC分类号： B01L3/02
CPC分类号： C12M35/00 , Y10T137/0396 , Y10T137/0971 , Y10T137/3115 , Y10T137/85978
摘要： A second valve is opened to combine an injection-pressure generating pressure with a maintaining pressure into an injection pressure. After the injection pressure is applied to a capillary to cause discharge of an object therefrom, an output pressure of a regulator is set to the injection pressure. Then, a first valve is opened to reapply the injection pressure to the capillary. The second valve is opened to combine a maintaining-pressure generating pressure with the injection pressure into the maintaining pressure. After the maintaining pressure is applied to the capillary to terminate the discharge of the object, the output pressure of the regulator is set to the maintaining pressure. Then, the first valve is opened to reapply the maintaining pressure to the capillary.
摘要翻译：打开第二阀，将注射压力产生压力与保持压力组合成注射压力。在向毛细管施加注射压力以使物体排出之后，将调节器的输出压力设定为注射压力。然后，打开第一阀，以将注射压力重新应用于毛细管。第二阀打开，将维持压力产生压力与喷射压力组合成维持压力。在对毛细管施加维持压力以终止物体的排出之后，将调节器的输出压力设定为保持压力。然后，打开第一个阀，以将保持压力重新应用到毛细管。

4. 发明申请

US20120111886A1 DISCHARGE VOLUME CONTROL METHOD, DISCHARGE PRESSURE CONTROL METHOD, AND MICROBODY FORMING METHOD 有权
标题翻译：放电量控制方法，放电压力控制方法和微型成形方法
公开(公告)号：US20120111886A1
公开(公告)日：2012-05-10
申请号：US13354808
申请日：2012-01-20
申请人： Tohru HARADA , Akihiko Yabuki , Kiyoshi Taninaka
发明人： Tohru HARADA , Akihiko Yabuki , Kiyoshi Taninaka
IPC分类号： B67D7/08
CPC分类号： C12M35/00 , Y10T137/0396 , Y10T137/0971 , Y10T137/3115 , Y10T137/85978
摘要： A second valve is opened to combine an injection-pressure generating pressure with a maintaining pressure into an injection pressure. After the injection pressure is applied to a capillary to cause discharge of an object therefrom, an output pressure of a regulator is set to the injection pressure. Then, a first valve is opened to reapply the injection pressure to the capillary. The second valve is opened to combine a maintaining-pressure generating pressure with the injection pressure into the maintaining pressure. After the maintaining pressure is applied to the capillary to terminate the discharge of the object, the output pressure of the regulator is set to the maintaining pressure. Then, the first valve is opened to reapply the maintaining pressure to the capillary.
摘要翻译：打开第二阀，将注射压力产生压力与保持压力组合成注射压力。在向毛细管施加注射压力以使物体排出之后，将调节器的输出压力设定为注射压力。然后，打开第一阀，以将注射压力重新应用于毛细管。第二阀打开，将维持压力产生压力与喷射压力组合成维持压力。在对毛细管施加维持压力以终止物体的排出之后，将调节器的输出压力设定为保持压力。然后，打开第一个阀，以将保持压力重新应用到毛细管。

5. 发明授权

US08859224B2 Microinjection device and microinjection method 有权
标题翻译：显微注射装置和显微注射法
公开(公告)号：US08859224B2
公开(公告)日：2014-10-14
申请号：US11785410
申请日：2007-04-17
申请人： Kiyoshi Taninaka , Jun Sasaki , Akihiko Yabuki
发明人： Kiyoshi Taninaka , Jun Sasaki , Akihiko Yabuki
IPC分类号： C12Q1/02 , C12M1/42 , C12M1/34
CPC分类号： C12M35/00 , C12M41/00 , C12M41/40 , C12M41/44
摘要： A fluorescence-intensity detecting unit detects fluorescence intensity by injecting a first solution containing a fluorescent reagent into a second solution that does not form an interface with the first solution through an injecting member. A calculating unit calculates the injection amount of the first solution from the fluorescence intensity based on a correlation between fluorescence intensities and injection amounts measured in advance. A computing unit obtains a correlation between an injection amount, pressure and pressurizing time based on the calculated injection amount. An adjusting unit adjusts the amount of the first solution to be injected into the endoplasmic reticulum by controlling pressure and pressurizing time based on the obtained correlation.
摘要翻译：荧光强度检测单元通过将含有荧光试剂的第一溶液通过注射部件注入到与第一溶液不形成界面的第二溶液中来检测荧光强度。计算单元基于荧光强度和预先测量的注射量之间的相关性，从荧光强度计算第一溶液的注射量。计算单元基于所计算的喷射量来获得喷射量，压力和加压时间之间的相关性。调整单元通过基于获得的相关性来控制压力和加压时间来调节要注入内质网的第一溶液的量。

6. 发明授权

US08012417B2 Solution discharging method and solution discharging device 有权
标题翻译：溶液排放法和溶液排出装置
公开(公告)号：US08012417B2
公开(公告)日：2011-09-06
申请号：US11797630
申请日：2007-05-04
申请人： Kiyoshi Taninaka , Jun Sasaki , Akihiko Yabuki
发明人： Kiyoshi Taninaka , Jun Sasaki , Akihiko Yabuki
IPC分类号： B01L3/02
CPC分类号： C12N15/89 , C12M35/00 , C12M41/12 , G01N35/1016 , G01N35/1095 , Y10T137/218 , Y10T137/2218
摘要： A microinjection device includes a pressure pump and a regulator that is connected to the pressure pump and that maintains constant pressure. A regulating chamber is connected to the regulator and an internal pressure of the regulating chamber is maintained to a predetermined pressure. A valve is connected to the regulating chamber and a hollow capillary is connected to the valve. An operator opens/closes the valve in the process of injecting material in a cell.
摘要翻译：显微注射装置包括压力泵和调节器，其连接到压力泵并保持恒定的压力。调节室连接到调节器，并且调节室的内部压力保持在预定压力。阀连接到调节室，中空毛细管连接到阀。操作者在将电池注入材料的过程中打开/关闭阀门。

7. 发明申请

US20080206802A1 Microinjection device and microinjection method 有权
标题翻译：显微注射装置和显微注射法
公开(公告)号：US20080206802A1
公开(公告)日：2008-08-28
申请号：US11785410
申请日：2007-04-17
申请人： Kiyoshi Taninaka , Jun Sasaki , Akihiko Yabuki
发明人： Kiyoshi Taninaka , Jun Sasaki , Akihiko Yabuki
IPC分类号： C12Q1/02 , C12M1/36
CPC分类号： C12M35/00 , C12M41/00 , C12M41/40 , C12M41/44
摘要： A fluorescence-intensity detecting unit detects fluorescence intensity by injecting a first solution containing a fluorescent reagent into a second solution that does not form an interface with the first solution through an injecting member. A calculating unit calculates the injection amount of the first solution from the fluorescence intensity based on a correlation between fluorescence intensities and injection amounts measured in advance. A computing unit obtains a correlation between an injection amount, pressure and pressurizing time based on the calculated injection amount. An adjusting unit adjusts the amount of the first solution to be injected into the endoplasmic reticulum by controlling pressure and pressurizing time based on the obtained correlation.
摘要翻译：荧光强度检测单元通过将含有荧光试剂的第一溶液通过注射部件注入到与第一溶液不形成界面的第二溶液中来检测荧光强度。计算单元基于荧光强度和预先测量的注射量之间的相关性，从荧光强度计算第一溶液的注射量。计算单元基于所计算的喷射量来获得喷射量，压力和加压时间之间的相关性。调整单元通过基于获得的相关性来控制压力和加压时间来调节要注入内质网的第一溶液的量。

8. 发明申请

US20070264162A1 Solution discharging method and solution discharging device 有权
标题翻译：溶液排放法和溶液排出装置
公开(公告)号：US20070264162A1
公开(公告)日：2007-11-15
申请号：US11797630
申请日：2007-05-04
申请人： Kiyoshi Taninaka , Jun Sasaki , Akihiko Yabuki
发明人： Kiyoshi Taninaka , Jun Sasaki , Akihiko Yabuki
IPC分类号： B01L3/02
CPC分类号： C12N15/89 , C12M35/00 , C12M41/12 , G01N35/1016 , G01N35/1095 , Y10T137/218 , Y10T137/2218
摘要： A microinjection device includes a pressure-pump and a regulator that is connected to the pressure pump and that maintains constant pressure. A regulating chamber is connected to the regulator and an internal pressure of the regulating chamber is maintained to a predetermined pressure. A valve is connected to the regulating chamber and a hollow capillary is connected to the valve. An operator opens/closes the valve in the process of injecting material in a cell.
摘要翻译：显微注射装置包括压力泵和调节器，其连接到压力泵并保持恒定的压力。调节室连接到调节器，并且调节室的内部压力保持在预定压力。阀连接到调节室，中空毛细管连接到阀。操作者在将电池注入材料的过程中打开/关闭阀门。

9. 发明申请

US20080299647A1 Cell capturing plate, microinjection apparatus, and method of producing cell capturing plate 审中-公开
标题翻译：细胞捕获板，显微注射装置和细胞捕获板的制造方法
公开(公告)号：US20080299647A1
公开(公告)日：2008-12-04
申请号：US12155003
申请日：2008-05-28
申请人： Akio Ito , Akihiko Yabuki , Daisuke Uchida , Satoru Sakai , Sachihiro Youoku
发明人： Akio Ito , Akihiko Yabuki , Daisuke Uchida , Satoru Sakai , Sachihiro Youoku
IPC分类号： C12M1/00 , B44C1/22
CPC分类号： C12M35/00 , C12M23/12 , C12M25/06
摘要： A cell capturing plate has a flat surface, an opening formed in one surface of the flat surface and supporting the cell therein, and a through-hole penetrating from the opening to the other surface of the flat surface and having a cross section parallel to the flat surface smaller than that of the opening.
摘要翻译：细胞捕获板具有平坦表面，形成在平坦表面的一个表面中并且将细胞支撑在其中的开口以及从开口延伸到平坦表面的另一个表面的通孔，并且具有与平面比开口小。

10. 发明申请

US20080268540A1 Microinjection apparatus, trap plate and microinjection method 失效
标题翻译：显微注射装置，捕获板和显微注射法
公开(公告)号：US20080268540A1
公开(公告)日：2008-10-30
申请号：US12081994
申请日：2008-04-24
申请人： Akio Ito , Akihiko Yabuki , Daisuke Uchida , Satoru Sakai , Sachihiro Youoku
发明人： Akio Ito , Akihiko Yabuki , Daisuke Uchida , Satoru Sakai , Sachihiro Youoku
IPC分类号： C12N15/00 , C12M1/00
CPC分类号： C12M35/00 , C12M25/06
摘要： A microinjection apparatus has a trap plate which traps at least one cell, so as to fix a position thereof, a capillary needle which injects a substance into the cell trapped by the trap plate, a movement mechanism portion which thrusts a distal end of the capillary needle onto a in a lengthwise direction of the needle, and which withdraws the capillary needle in the lengthwise direction thereof, and a discharge control portion which discharges the substance from the distal end of the capillary needle, when the capillary needle has been withdrawn to a predetermined position by the movement mechanism portion.
摘要翻译：显微注射装置具有捕获板，其捕集至少一个细胞，以固定其位置，将物质注入被捕获板捕获的细胞的毛细管针;推动毛细管的远端的移动机构部分针头在针的长度方向上朝向长度方向退出毛细针，并且当毛细管针已经被抽出到一个位置时从毛细针的远端排出物质的排出控制部分通过移动机构部分的预定位置。

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