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1. 发明授权

US4842997A Enhanced luminescent and luminometric assay 失效
标题翻译：增强发光和光度测定
公开(公告)号：US4842997A
公开(公告)日：1989-06-27
申请号：US551985
申请日：1983-10-27
申请人： Timothy J. N. Carter , Carol J. Groucutt , Richard A. W. Stott , Gary H. G. H. Thorpe , Thomas P. Whitehead
发明人： Timothy J. N. Carter , Carol J. Groucutt , Richard A. W. Stott , Gary H. G. H. Thorpe , Thomas P. Whitehead
IPC分类号： G01N21/76 , C12Q1/28 , G01N33/58
CPC分类号： C12Q1/28 , G01N33/581 , Y10S435/81 , Y10S435/968
摘要： Assays, e.g. immunoassays, based on the chemiluminescent reaction between a peroxidase enzyme, e.g. horse radish peroxidase, a chemiluminescent 2,3-dihydro-1,4-phthalazinedione, e.g. luminol or isoluminol and an oxidant, e.g. hydrogen peroxide or a perborate, are improved by carrying out the reaction in the presence of a 6-hydroxybenzothiazole, especially 6-hydroxybenzothiazole itself or firefly luciferin. Light emission from the reaction is significantly enhanced by the 6-hydroxybenzothiazole.
摘要翻译： PCT No.PCT / GB83 / 00058 Sec。 371日期1983年10月27日第 102（e）日期1983年10月27日PCT提交1983年2月25日PCT公布。公开号WO83 / 03104 日期：1983年9月15日。基于过氧化物酶之间的化学发光反应的免疫测定。辣根过氧化物酶，化学发光2,3-二氢-1,4-酞嗪二酮，例如鲁米诺或异鲁米诺和氧化剂，例如过氧化氢或过硼酸盐，通过在6-羟基苯并噻唑，特别是6-羟基苯并噻唑本身或萤火虫荧光素的存在下进行反应得到改善。由6-羟基苯并噻唑显着增强反应的光发射。

2. 发明授权

US4879097A Dispensing device and recording apparatus 失效
标题翻译：点胶装置和记录装置
公开(公告)号：US4879097A
公开(公告)日：1989-11-07
申请号：US848134
申请日：1986-04-04
申请人： Thomas P. Whitehead , Gary H. G. H. Thorpe , Larry J. Kricka , John E. C. Gibbons , Roger A. Bunce
发明人： Thomas P. Whitehead , Gary H. G. H. Thorpe , Larry J. Kricka , John E. C. Gibbons , Roger A. Bunce
IPC分类号： G01N35/00 , B01L3/02 , G01N21/76
CPC分类号： G01N21/76 , B01L3/021 , G01N2201/04
摘要： A recording apparatus has a holder for a plurality of reaction vessels including a support plate (11) having an array of holes (15) therein for receiving an array of reaction vessels (16), a housing (10) for receiving the holder and being sealed to prevent entry of stray light, an openable cover (24, 249) for the housing, a film back (12) for holding a photographic film adjacent the underside of the plate (11), and a removable shutter (13) for interposition between the film and the support plate.

3. 发明授权

US5395755A Antioxidant assay 失效
标题翻译：抗氧化测定
公开(公告)号：US5395755A
公开(公告)日：1995-03-07
申请号：US949518
申请日：1992-11-25
申请人： Gary H. G. H. Thorpe , Thomas P. Whitehead
发明人： Gary H. G. H. Thorpe , Thomas P. Whitehead
IPC分类号： G01N21/76 , C12Q1/28 , G01N33/50 , G01N33/52 , G01N33/542 , G01N33/58 , G01N33/82
CPC分类号： G01N33/582 , C12Q1/28 , G01N33/52 , G01N33/542 , Y10S435/81 , Y10S435/968
摘要： A method of assay of antioxidant activity of a biological fluid, which comprises monitoring the change exerted by the sample on a progressing luminescent reaction.
摘要翻译： PCT No.PCT / GB91 / 00931 Sec。 371日期：1992年11月25日 102（e）日期1992年11月25日PCT提交1991年6月11日PCT公布。 WO91 / 19979 PCT出版物日本1991年12月26日。一种测定生物液体的抗氧化活性的方法，其包括监测样品对进行的发光反应所产生的变化。

4. 发明授权

US4598044A Enhanced luminescent or luminometric assay 失效
标题翻译：增强发光或发光测定
公开(公告)号：US4598044A
公开(公告)日：1986-07-01
申请号：US578783
申请日：1984-02-09
申请人： Larry J. Kricka , Gary H. G. H. Thorpe , Thomas P. Whitehead
发明人： Larry J. Kricka , Gary H. G. H. Thorpe , Thomas P. Whitehead
IPC分类号： C12Q1/28 , G01N33/533 , G01N33/58 , G01N33/53 , C12Q1/66 , G01N33/564
CPC分类号： G01N33/533 , C12Q1/28 , G01N33/581 , Y10S435/81 , Y10S435/962 , Y10S435/968 , Y10S435/975 , Y10S436/80 , Y10S436/817
摘要： A phenolic compound is used to enhance the sensitivity of a luminescent reaction such as carried out in an immunoassay between a peroxidase enzyme, an oxidant, and a chemiluminescent 2,3-dihydro-1,4-phthalazinedione. Preferably, the phenolic compound is 4-iodophenol, 4-phenylphenol or 2-chloro-4-phenylphenol. In the preferred embodiment, horseradish peroxidase is coupled to an antibody to the substance to be assayed.
摘要翻译：酚类化合物用于增强发光反应的敏感性，例如在过氧化物酶，氧化剂和化学发光2,3-二氢-1,4-酞嗪二酮之间的免疫测定中进行的。优选地，酚类化合物是4-碘苯酚，4-苯基苯酚或2-氯-4-苯基苯酚。在优选的实施方案中，辣根过氧化物酶与待测物质的抗体偶联。

5. 发明授权

US5580791A Assay of water pollutants 失效
标题翻译：水污染物测定
公开(公告)号：US5580791A
公开(公告)日：1996-12-03
申请号：US449249
申请日：1995-05-24
申请人： Gary H. G. H. Thorpe , Thomas P. Whitehead
发明人： Gary H. G. H. Thorpe , Thomas P. Whitehead
IPC分类号： C12Q1/28 , G01N21/76 , G01N21/78 , G01N33/18
CPC分类号： G01N21/76 , G01N33/18 , G01N33/1846
摘要： A method of assay of antioxidant activity of a sample of water, which comprises monitoring the change exerted by the sample on a luminescent reaction.
摘要翻译：一种测定水样抗氧化活性的方法，其包括监测样品对发光反应的变化。

6. 发明授权

US4729950A Enhanced luminescent or luminometric assay 失效
标题翻译：增强发光或发光测定
公开(公告)号：US4729950A
公开(公告)日：1988-03-08
申请号：US760036
申请日：1985-07-29
申请人： Larry J. Kricka , Angela M. O'Toole , Gary H. G. H. Thorpe , Thomas P. Whitehead
发明人： Larry J. Kricka , Angela M. O'Toole , Gary H. G. H. Thorpe , Thomas P. Whitehead
IPC分类号： G01N33/52 , C12Q1/28 , G01N21/76 , G01N33/532 , G01N33/535
CPC分类号： G01N33/535 , C12Q1/28 , Y10S435/81 , Y10S435/968 , Y10S435/975 , Y10S436/80
摘要： It has been a problem that the chemiluminescent emission produced by the peroxidase-catalyzed oxidation of a 2,3-dihydro-1,4-phthalazinedione is often weak or gives a poor signal-background ratio and therefore does not give the high degree of sensitivity required for assay purposes, particularly for assay of peroxidase used to label a reagent for example in ELISA. The present invention solves the problem by the finding the certain narrowly defined aromatic amines are enhancers of the reaction and accordingly provides for their use in an assay and kit. The amines are of general formula (I) ##STR1## wherein the R symbols (that is, R, R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5 and R.sup.6) have any of the meanings (a) to (j) given below, all other R symbols in each meaning are hydrogen atoms and the fused rings are to be read in the same configurational sense as formula I:(a) R=R.sup.1 =CH.sub.3 ; R.sup.4 = ##STR2## (b) R.sup.4 = ##STR3## cyclohexyl, or alkyl or alkoxy having 1 to 4 carbon atoms (c) R.sup.2, R.sup.3 and R.sup.4 together represent the polycyclic fused ring system: ##STR4## (d) R.sup.3 and R.sup.4 together represent the fused ring or ring system ##STR5## (e) R.sup.3 and R.sup.4 together represent the fused ring system ##STR6## and R.sup.5 and R.sup.6 together represent the fused ring ##STR7## (f) R.sup.2 =NH.sub.2 and R.sup.4 = ##STR8## (g) R.sup.2 =R.sup.6 =CH.sub.3 and R.sup.4 = ##STR9## (h) R.sup.3 =C.sub.2 H.sub.5 (i) R.sup.2 =R.sup.4 =CH.sub.3 and(j) R.sup.3 =R.sup.4 =CH.sub.3.
摘要翻译：由2,3-二氢-1,4-酞嗪二酮的过氧化物酶催化氧化产生的化学发光辐射常常较弱或信号背景差较弱，因此不会产生高灵敏度的问题用于测定目的，特别是用于测定用于标记例如ELISA中的试剂的过氧化物酶。本发明通过发现某些狭义定义的芳族胺是反应的增强剂来解决该问题，因此提供了它们在测定和试剂盒中的应用。胺具有通式（I）其中R符号（即R，R 1，R 2，R 3，R 4，R 5和R 6）具有给定的（a）至（j）在下文中，每个含义中的所有其他R符号是氢原子，并且稠合环将以与式I相同的构型意义读取：（a）R = R 1 = CH 3; （c）R2 = R3，R4和R4分别代表多环稠环体系：（R）R 4 = （e）R3和R4一起代表稠合环系统，而R 5和R 6一起代表稠环，（f）R2 = NH2，R4 = （g）R2 = R6 = CH3，R4 =（h）R3 = C2H5（i）R2 = R4 = CH3，（j）R3 = R4 = CH3。

7. 发明授权

US4593728A Dispensing device and recording apparatus 失效
标题翻译：点胶装置和记录装置
公开(公告)号：US4593728A
公开(公告)日：1986-06-10
申请号：US626856
申请日：1984-06-27
申请人： Thomas P. Whitehead , Gary H. G. H. Thorpe , Larry J. Kricka , John E. C. Gibbons , Roger A. Bunce
发明人： Thomas P. Whitehead , Gary H. G. H. Thorpe , Larry J. Kricka , John E. C. Gibbons , Roger A. Bunce
IPC分类号： G01N35/00 , B01L3/02 , G01N21/76 , B65B3/04
CPC分类号： G01N21/76 , B01L3/021 , G01N2201/04
摘要： A dispensing device, for dispensing reagent into an array of reaction wells (16) in a photographic recording apparatus, comprises an array of tubes (28) which are mounted in a support (23). The support (23) contains a common chamber (29) into which the tops of all the tubes (28) open. The chamber (29) is connected with atmosphere via an opening (32) which is sealable manually to retain liquid in the tubes (28) when the latter are immersed in a reservoir (40) and which is unsealed to dispense the liquid. The recording apparatus has a housing (10) closable at the top by part of the support (23). A plate (11) having an array of holes (15) therethrough rests on a shutter (13) disposed above a photographic film holder (12). The plate (11) carries the reaction wells (16) in the holes (15).
摘要翻译： PCT No.PCT / GB83 / 00287 Sec。 371日期1984年6月27日第 102（e）日期1984年6月27日PCT提交1983年11月14日PCT公布。出版物WO84 / 02090 日期：1984年6月7日。一种用于将试剂分配到摄影记录装置中的反应井（16）阵列中的分配装置，包括安装在支撑件（23）中的管阵列（28）。支撑件（23）包含公共室（29），所有管（28）的顶部打开。室（29）经由可手动密封的开口（32）与大气连接，当开口（32）浸入储存器（40）中时，将液体保持在管（28）中，并且其未被密封以分配液体。该记录装置具有一个壳体（10），可以在顶部由支撑件（23）的一部分封闭。具有穿过孔阵列（15）的板（11）搁置在设置在照相胶片保持器（12）上方的挡板（13）上。板（11）在孔（15）中承载反应孔（16）。

8. 发明授权

US4366118A Apparatus and method for luminescent determination of concentration of an analyte in a sample 失效
标题翻译：用于发光测定样品中分析物浓度的装置和方法
公开(公告)号：US4366118A
公开(公告)日：1982-12-28
申请号：US189930
申请日：1980-02-14
申请人： Roger A. Bunce , Timothy J. N. Carter , John H. Kennedy , Larry J. Kricka , Thomas P. Whitehead
发明人： Roger A. Bunce , Timothy J. N. Carter , John H. Kennedy , Larry J. Kricka , Thomas P. Whitehead
IPC分类号： G01N33/58 , G01N21/01 , G01N21/76
CPC分类号： G01N21/76
摘要： The apparatus comprises a movable member (10) which carries a plurality of cuvettes (11) containing material under test. Control means cause the member (10) to be moved to bring the cuvettes (11) successively into register with a photomultiplier (16), and in that position reagent is automatically added to a cuvette, giving rise to a luminescent reaction. Light from the reaction is detected by the photo-multiplier (16) and the resultant signal processed and recorded. The control means is triggered to stop the member (10), add reagent, record the signal from the luminescent reaction, and restart the member, from a photocell (28) which is illuminated through the member (10) only when a cuvette (11) is in register with the photomultiplier (16).According to the method, sample material containing the analyte for determination is mixed with a known amount or concentration of the same analyte labelled with a substance which is capable of taking part in a luminescent reaction. The mixture is then reacted with a known amount of another substance capable of reacting with the analyte and giving rise to a luminescent reaction with the labelling substance. From quantitative observation of the light from the luminescent reaction the amount or concentration of the analyte in the sample may be deduced.
摘要翻译： PCT No.PCT / GB79 / 00102 Sec。 371日期1980年2月14日 102（e）1980年2月14日PCT PCT。出版物WO80 / 00100 PCT 日期为1980年1月24日。该装置包括可移动构件（10），其携带包含被测材料的多个比色皿（11）。控制装置使得构件（10）移动以使比色杯（11）依次与光电倍增器（16）对准，并且在该位置，试剂自动添加到比色杯中，引起发光反应。通过光电倍增器（16）检测反应的光，并对所得信号进行处理和记录。控制装置被触发以停止构件（10），添加试剂，记录来自发光反应的信号，并从仅通过构件（10）照射的光电池（28）重新启动构件，只有当比色杯（11 ）与光电倍增管（16）对齐。根据该方法，将含有用于测定的分析物的样品材料与能够参与发光反应的物质标记的相同分析物的已知量或浓度混合。然后将混合物与已知量的能够与分析物反应并产生与标记物质的发光反应的另一种物质反应。通过定量观察发光反应的光，可以推导出样品中分析物的量或浓度。

9. 发明授权

US4227886A Testing a cuvette for cleanliness 失效
标题翻译：测试比色杯清洁
公开(公告)号：US4227886A
公开(公告)日：1980-10-14
申请号：US796612
申请日：1977-05-13
申请人： David G. Bullock , Roger A. Bunce , Ian R. Clark , Ian D. Duff , Geoffrey S. Greaves , Peter H. Lloyd , Ann M. Peters , Thomas P. Whitehead , Peter Wilding
发明人： David G. Bullock , Roger A. Bunce , Ian R. Clark , Ian D. Duff , Geoffrey S. Greaves , Peter H. Lloyd , Ann M. Peters , Thomas P. Whitehead , Peter Wilding
IPC分类号： G01N21/15 , B01L99/00 , B08B9/46 , G01N21/90 , G01N33/00 , G01N35/02 , B08B7/00 , B08B5/04 , G01N29/02
CPC分类号： G01N21/9018 , B01L99/00 , G01N2033/0081 , Y10T436/114998
摘要： In testing for cleanliness a cuvette having translucent wall portions, the cuvette is preferably partially filled with pure water and then a beam of optical radiation is directed at a said wall portion, the intensity of radiation which has passed through the cuvette by direct transmission or by scattering is detected, and the state of cleanliness of the cuvette is determined from a comparison between the detected optical intensity and a criterion indicative of cuvette cleanliness.The invention finds particular application to testing cuvettes for cleanliness which have been washed in a laundry of an automatic blood analysing machine.
摘要翻译：在清洁度测试中，具有半透明壁部分的比色杯，比色杯优选地部分地填充有纯水，然后光束辐射指向所述壁部分，通过直接透射或通过直接透射通过比色杯的辐射强度检测到散射，并且通过检测到的光强度和指示比色皿清洁度的标准之间的比较来确定比色杯的清洁度。本发明特别适用于在自动血液分析机的衣物中洗涤的清洁度的试管。

10. 发明授权

US4356722A Apparatus for testing a liquid sample 失效
标题翻译：用于测试液体样品的装置
公开(公告)号：US4356722A
公开(公告)日：1982-11-02
申请号：US203572
申请日：1980-11-05
申请人： Roger A. Bunce , John H. Kennedy , Larry J. Kricka , Thomas P. Whitehead
发明人： Roger A. Bunce , John H. Kennedy , Larry J. Kricka , Thomas P. Whitehead
IPC分类号： G01N35/00 , G01N35/02
CPC分类号： G01N35/02 , G01N35/00
摘要： Apparatus for testing at least one liquid sample, by immersion therein of a probe for coaction between said probe and sample, having a reloadable sample carrier for at least one sample container; a reloadable probe carrier for at least one probe; a frame having locating means for relatively locating the sample carrier and the probe carrier laterally in the frame, parallel motion means for guiding the probe longitudinally into the sample container and stop means for locating the probe at a predetermined distance into the sample container, locking means preventing the probe from being withdrawn more than a predetermined distance in the outward direction from the sample container, timing and control means for releasing said locking means after a predetermined time, and self acting withdrawal means for withdrawing the probe and probe carrier to a position clear of the sample container; the said sample carrier and probe carrier being arranged so as to be severally removable away from the said frame.
摘要翻译：用于测试至少一种液体样品的装置，通过浸入其中用于所述探针和样品之间的共同作用的探针，具有用于至少一个样品容器的可重新加载的样品载体; 用于至少一个探针的可重新加载的探针载体; 具有用于将样品载体和探针载体横向地相对定位在框架中的定位装置的框架，用于将探针纵向引导到样品容器中的平行运动装置和用于将探针定位在预定距离进入样品容器的停止装置，锁定装置防止探针从样品容器向外的方向被抽出超过预定距离，在预定时间之后释放所述锁定装置的定时和控制装置，以及用于将探针和探针载体抽出到位置的自动提取装置的样品容器; 所述样品载体和探针载体被布置成从所述框架分开移除。

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