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    • 1. 发明授权
    • Light detecting optical device
    • 光检测光学装置
    • US06714297B1
    • 2004-03-30
    • US09646031
    • 2001-01-11
    • Thomas RuckstuhlStefan Seeger
    • Thomas RuckstuhlStefan Seeger
    • G01N2164
    • G01N21/6452G01N21/552G01N21/648G01N33/54373G01N2021/6478G02B6/4206G02B6/4298
    • A fluorescing molecule is bound to an microscopic slide (24). The microscopic slide (24) is coupled to a first end face of an optical waveguide (1) by means of an immersion oil. The optical waveguide also possesses a shell surface (3) having the shape of a paraboloid of revolution, and a second end face (4). The molecule is located at the focal point of the paraboloid of revolution. Essentially the entire light of the molecule radiated into the half-space facing the optical waveguide (1) enters the optical waveguide (1). In particular, evanescent radiation (7) also enters the optical waveguide (1). This is totally internally reflected at the shell surface (3) having the shape of a paraboloid of revolution, emerges collimated from the second end face (4), and reaches a detector (30). In this way, essentially the entire evanescent radiation of the molecule can be registered. The optical arrangement thus improves the light-collecting efficiency for evanescent radiation, and thus the selective sensitivity of detection for molecules near the surface for fluorescence-based biosensors.
    • 荧光分子与微观载玻片结合(24)。 微型滑块(24)借助于浸油将其耦合到光波导(1)的第一端面。 光波导还具有具有旋转抛物面形状的壳表面(3)和第二端面(4)。 该分子位于革命抛物面的焦点。 基本上辐射到面向光波导(1)的半空间中的分子的整个光进入光波导(1)。 特别地,渐逝辐射(7)也进入光波导(1)。 这是在具有旋转抛物面形状的壳体表面(3)内部反射出来的,从第二端面(4)平行出来,到达检测器(30)。 以这种方式,基本上可以记录分子的整个ev逝辐射。 因此,光学装置改善了渐逝辐射的聚光效率,从而提高了荧光基生物传感器表面附近分子的检测灵敏度。
    • 2. 发明申请
    • RECEPTACLE AND METHOD FOR THE DETECTION OF FLUORESCENCE
    • 荧光检测的检测方法
    • US20100136709A1
    • 2010-06-03
    • US12598397
    • 2008-06-27
    • Thomas RuckstuhlStefan Seeger
    • Thomas RuckstuhlStefan Seeger
    • G01N21/62G01N21/00
    • G01N21/0303B01L3/5027B01L3/5082B01L3/5085B01L2300/0654G01N21/645G01N21/6452G01N21/6458G01N2201/0642G02B2207/113
    • The invention relates to a liquid receptacle comprising a bottom and sidewalls for holding a liquid. The bottom encompasses a flat sensor surface that is in contact with the liquid when the receptacle is filled, a light-incident area that is located below the sensor surface and is suitable for focusing light onto the sensor surface, a light emergence area, and a cover area that is suitable for reflecting light from the sensor surface such that the light can emerge through the light emergence area. The invention further relates to a method for qualitatively or quantitatively determining an analyte in such a liquid receptacle. In said method, excitation light is focused onto the sensor surface via the light-incident area such that a luminescent marker which characterizes the analyte is excited, and the generated luminescence is then reflected onto the cover surface and is detected after emerging through the light emergence area. The invention also relates to an analysis device comprising a holder for a liquid receptacle, a light source that is disposed such that the light thereof can be focused onto the sensor surface of the liquid receptacle via the light-incident area, and a detector which is arranged in such a way as to be able to detect the light emerging from the light emergence area of the liquid receptacle.
    • 本发明涉及一种液体容器,其包括用于保持液体的底部和侧壁。 底部包括当容器被填充时与液体接触的平坦传感器表面,位于传感器表面下方并且适于将光聚焦到传感器表面上的光入射区域,光出射区域和 覆盖区域,其适于反射来自传感器表面的光,使得光可以通过光出射区域出射。 本发明还涉及用于定性或定量地测定这种液体容器中的分析物的方法。 在所述方法中,激发光通过光入射区域聚焦到传感器表面上,使得表征分析物的发光标记物被激发,并且所产生的发光然后被反射到盖表面上,并且在通过光出射出现之后被检测 区。 本发明还涉及一种分析装置,其包括用于液体容器的保持器,设置成使得其光能够经由光入射区域聚焦到液体容器的传感器表面上的光源,以及检测器 以能够检测从液体容器的光出射区域出射的光的方式布置。
    • 4. 发明授权
    • Superhydrophobic coating
    • 超疏水涂层
    • US08586693B2
    • 2013-11-19
    • US12983987
    • 2011-01-04
    • Jan ZimmermannStefan SeegerGeorg ArtusStefan Jung
    • Jan ZimmermannStefan SeegerGeorg ArtusStefan Jung
    • C08G77/00
    • C09D4/00Y10T428/2438Y10T428/24421Y10T428/26Y10T428/265Y10T428/2924Y10T428/2929Y10T428/2931Y10T428/2962Y10T428/2964Y10T428/31612Y10T428/31663Y10T442/218C08G77/04
    • A composition for coating comprising at least one compound of formula I and optionally at least one compound of formula II RaSi(R1)n(X1)3-n  I RbSi(R2)m(X2)3-m  II wherein Ra is a straight-chain or branched C(1-24) alkyl group, Rb is an aromatic group, such as an optionally substituted carbocyclic and heterocyclic group comprising five-, six- or ten-membered ring systems, which is linked by a single covalent bond or a spacer unit, such as a straight-chain or branched alkyl residue having 1 to 8 carbon atoms, to the Si— atom, R1 and R2 are independently of each other a lower alkyl group, such as a straight chain and branched hydrocarbon radical having 1 to 6 carbon atoms, X1 and X2 are independently of each other a hydrolysable group, such as a halogen or an alkoxy group and n, m are independently of each other 0 or 1, with the proviso that if n and m are independently of each other 0 or 1, X may represent the same or different groups.
    • 用于涂层的组合物,其包含至少一种式I化合物和任选的至少一种式II的化合物RaSi(R1)n(X1)3-nI RbSi(R2)m(X2)3-mII,其中Ra是直链 或支链C(1-24)烷基,Rb是芳族基团,例如任选取代的碳环和杂环基,其包含通过单个共价键或间隔基连接的五,六或十元环体系 单元,例如具有1至8个碳原子的直链或支链烷基,对于Si原子,R 1和R 2彼此独立地是低级烷基,例如直链和支链烃基,其具有1至 6个碳原子,X 1和X 2彼此独立地是可水解基团,例如卤素或烷氧基,n,m彼此独立地为0或1,条件是如果n和m彼此独立地 0或1,X可以表示相同或不同的基团。
    • 6. 发明授权
    • Method for DNA- or RNA-sequencing
    • DNA或RNA测序方法
    • US06524829B1
    • 2003-02-25
    • US09806320
    • 2001-06-29
    • Stefan Seeger
    • Stefan Seeger
    • C12P1934
    • C12Q1/6869Y10T436/143333C12Q2565/518C12Q2563/103C12Q2523/319
    • The invention relates to methods for the base sequencing of deoxyribonucleic acid or ribonucleic acid, comprising the following steps: (1) immobilising DNA or RNA single strands on a planar support; (2) focussing a laser beam on a single, immobilised single strand; (3) producing DNA or RNA complimentary strand of said immobilised, focused single strand by adding a solution containing (i) at least one luminescence-tagged nucleotide on the bases of adenine, cytosine, guanine and thymine for producing a DNA or RNA complementary strand or at least one luminescence-tagged nucleotide of the bases adenine, cytosine, guanine and uracil for producing an RNA complementary strand and (ii) a polymerase, each insertion of a luminescence-tagged nucleotide into the complementary strand being detected with a single-molecule detector and the luminescence signal of the previous luminescence-tagged nucleotide being deleted before the next luminescence-tagged nucleotide is inserted.
    • 本发明涉及脱氧核糖核酸或核糖核酸的碱基测序方法,包括以下步骤:(1)将DNA或RNA单链固定在平面载体上; (2)将激光束聚焦在单个固定的单股上; (3)通过加入含有(i)在腺嘌呤,胞嘧啶,鸟嘌呤和胸腺嘧啶的碱基上的至少一个发光标记的核苷酸以产生DNA或RNA互补链的溶液来产生所述固定的聚焦单链的DNA或RNA互补链 或用于产生RNA互补链的碱基腺嘌呤,胞嘧啶,鸟嘌呤和尿嘧啶的至少一个发光标记的核苷酸,和(ii)聚合酶,每个将引发标记的核苷酸插入互补链中,用单分子 检测器和先前发光标记的核苷酸的发光信号在插入下一个发光标记的核苷酸之前被删除。