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1. 发明授权

US4885252A Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from aspergillus nidulans 失效
标题翻译：重组DNA表达载体和编码来自构巢曲霉的异青霉素N合成酶的DNA化合物
公开(公告)号：US4885252A
公开(公告)日：1989-12-05
申请号：US133760
申请日：1987-12-14
申请人： Thomas D. Ingolia , Stephen W. Queener , Paul L. Skatrud , Barbara J. Wiegel
发明人： Thomas D. Ingolia , Stephen W. Queener , Paul L. Skatrud , Barbara J. Wiegel
IPC分类号： C07H21/04 , C12N1/14 , C12N1/20 , C12N1/21 , C12N9/00 , C12N15/00 , C12N15/09 , C12N15/54 , C12N15/70 , C12R1/19 , C12R1/66 , C12R1/745 , C12R1/80
CPC分类号： C12N9/93
摘要： DNA compounds and recombinant DNA expression vectors that encode and drive expression in recombinant host cells of the isopenicillin N synthetase activity of Aspergillus nidulans are useful to produce isopenicillin N synthetase and to improve the yield of .beta.-lactam-containing antibiotics from antibiotic-producing organisms. The isopenicillin N synthetase gene of A. nidulans can be isolated from plasmid pOGOO4, available from the Northern Regional Research Center under the accession number NRRL B-18171.

2. 发明授权

US06180361B2 Recombinant DNA expression vectors and DNA compounds that encode deacetoxycephalosporin C synthetase and deacetylcephalosporin C synthetase 失效
标题翻译：重组DNA表达载体和编码脱乙酰头孢菌素C合成酶和脱乙酰头孢菌素C合成酶的DNA化合物
公开(公告)号：US06180361B2
公开(公告)日：2001-01-30
申请号：US07283429
申请日：1988-12-12
申请人： Thomas D. Ingolia , Stephen W. Queener , Suellen M. Samson , Paul L. Skatrud
发明人： Thomas D. Ingolia , Stephen W. Queener , Suellen M. Samson , Paul L. Skatrud
IPC分类号： C12N1513
CPC分类号： C12N9/0071 , C12N15/52 , C12N15/70 , C12N15/80
摘要： The present invention provides DNA compounds that encode the expandase/hydroxylase enzyme of Cephalosporium acremonium. The compounds can be used to construct recombinant DNA expression vectors for a variety of host cells, including E. coli, Penicillium, and Cephalosporium.
摘要翻译：本发明提供编码头孢霉头孢菌的扩环酶/羟化酶的DNA化合物。该化合物可用于构建多种宿主细胞的重组DNA表达载体，包括大肠杆菌，青霉菌和头孢菌素。

3. 发明授权

US4892819A Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from penicillium chrysogenum 失效
标题翻译：重组DNA表达载体和编码来自产黄青霉的异青霉素N合成酶的DNA化合物
公开(公告)号：US4892819A
公开(公告)日：1990-01-09
申请号：US801523
申请日：1985-11-25
申请人： Lucinda G. Carr , Thomas D. Ingolia , Stephen W. Queener , Paul L. Skatrud
发明人： Lucinda G. Carr , Thomas D. Ingolia , Stephen W. Queener , Paul L. Skatrud
IPC分类号： C12N15/09 , C12N1/14 , C12N1/15 , C12N1/20 , C12N1/21 , C12N9/00 , C12N15/00 , C12N15/52 , C12N15/69 , C12N15/70 , C12N15/80 , C12N15/90 , C12P37/00 , C12R1/19 , C12R1/75 , C12R1/82
CPC分类号： C12N9/93 , C12N15/69 , C12N15/70 , C12N15/80 , C12N15/90
摘要： The present invention comprises novel DNA compounds that encode isopenicillin N synthetase. The invention also comprises methods, transformants, and polypeptides related to the novel DNA compounds. The novel isopenicillin N synthetase-encoding DNA, together with its associated transcription and translation activating sequence, was isolated from Penicillium chrysogenum. The isopenicillin N synthetase-encoding DNA can be used to construct novel E. coli expression vectors that drive expression of isopenicillin N synthetase in E. coli. The intact P. chrysogenum isopenicillin N synthetase-encoding DNA and associated transcription and translation activating sequence can also be used to construct expression vectors that drive expression of the isopenicillin N synthetase in P. chrysogenum and Cephalosporium acremonium. The transcription and translation activating sequence can be fused to a hygromycin phosphotransferase-encoding DNA segment and placed onto expression vectors that function in P. chrysogenum and C. acremonium. The transcription termination and mRNA polyadenylation signals of the P. chrysogenum isopenicillin N synthetase can be used to increase ultimate expression of a product encoded on a recombinant DNA vector.
摘要翻译：本发明包括编码异青霉素N合成酶的新型DNA化合物。本发明还包括与新型DNA化合物相关的方法，转化体和多肽。编码新颖的异青霉素N合成酶的DNA，连同其相关的转录和翻译活化序列，从产黄青霉中分离出来。编码异青霉素N合成酶的DNA可用于构建驱动大肠杆菌中异青霉素N合成酶表达的新型大肠杆菌表达载体。完整的产黄青霉素异青霉素N合成酶编码DNA和相关的转录和翻译活化序列也可用于构建表达产物，其驱动产黄青霉和顶头孢霉的异青霉素N合成酶的表达。转录和翻译激活序列可以与编码潮霉素磷酸转移酶的DNA片段融合，并置于表达载体中，该表达载体在产黄青霉和顶头孢霉中起作用。产黄青霉素异青霉素N合成酶的转录终止和mRNA聚腺苷酸化信号可用于增加编码在重组DNA载体上的产物的最终表达。

4. 发明授权

US4885251A Recombinant DNA expression vectors and DNA compounds which encode isopenicillin N synthetase 失效
标题翻译：编码异青霉素N合成酶的重组DNA表达载体和DNA化合物
公开(公告)号：US4885251A
公开(公告)日：1989-12-05
申请号：US895008
申请日：1986-08-08
申请人： Thomas D. Ingolia , Stephen W. Queener , Suellen M. Samson , Paul L. Skatrud , Otis W. Godfrey
发明人： Thomas D. Ingolia , Stephen W. Queener , Suellen M. Samson , Paul L. Skatrud , Otis W. Godfrey
IPC分类号： C12N15/52 , C12N9/00 , C12N15/69 , C12N15/70 , C12N15/74 , C12N15/80
CPC分类号： C12N15/80 , C12N15/69 , C12N9/93
摘要： The present invention comprises novel DNA compounds that encode isopenicillin N synthetase and also comprises related methods, transformants, and polypeptides. The novel isopenicillin N synthetase-encoding DNA, together with its associated transcriptional and translational activating sequence, was isolated from Cephalosporium acremonium and cloned into an E. coli cloning vector. The isopenicillin N synthetase-encoding DNA has been used to construct novel E. coli expression vectors that drive expression of a stable, active, and novel isopenicillin N synthetase in E. coli. The intact C. acremonium isopenicillin N synthetase-encoding DNA and associated transcriptional and translational activating sequence have also been used to construct C. acremonium expression vectors that drive expression of the isopenicillin N synthetase in C. acremonium. The C. acremonium transcriptional and translational activating sequence has further been fused to a hygromycin phosphotransferase-encoding DNA segment and placed onto C. acremonium expression vectors. Useful derivatives of the novel compounds and vectors are also described.
摘要翻译：本发明包括编码异青霉素N合成酶的新型DNA化合物，还包括相关方法，转化体和多肽。编码新的异青霉素N合成酶DNA，连同其相关的转录和翻译活化序列，从头孢霉头孢菌分离并克隆到大肠杆菌克隆载体中。编码异青霉素N合成酶的DNA已经用于构建新的大肠杆菌表达载体，其在大肠杆菌中驱动稳定的，活性的和新的异青霉素N合成酶的表达。编码完整的顶头孢霉素异青霉素N合成酶的DNA和相关的转录和翻译活化序列也被用于构建顶头孢霉表达载体，其驱动顶头孢霉中异青霉素N合成酶的表达。顶头孢霉转录和翻译激活序列进一步融合到编码潮霉素磷酸转移酶的DNA片段并置于顶头孢霉表达载体上。还描述了新化合物和载体的有用衍生物。

5. 发明授权

US4762786A Vectors and conditions which allow genetic transformation of cephalosporium 失效
标题翻译：允许头孢菌的遗传转化的载体和条件
公开(公告)号：US4762786A
公开(公告)日：1988-08-09
申请号：US654919
申请日：1984-09-27
申请人： Jerry L. Chapman, Jr. , Thomas D. Ingolia , Kevin R. Kaster , Stephen W. Queener , Paul L. Skatrud
发明人： Jerry L. Chapman, Jr. , Thomas D. Ingolia , Kevin R. Kaster , Stephen W. Queener , Paul L. Skatrud
IPC分类号： C12N15/09 , C12N1/14 , C12N1/20 , C12N9/12 , C12N15/63 , C12N15/80 , C12R1/19 , C12R1/465 , C12R1/745 , C12N15/00 , C12P21/00
CPC分类号： C12N9/12 , C12N15/63 , C12N15/80 , Y10S435/886 , Y10S435/925 , Y10S435/926
摘要： A method for transforming Cephalosporium and other lower eukaryotes is disclosed. The method involves inserting a recombinant DNA cloning vector comprising a Saccharomyces cerevisiae transcriptional and translational activating sequence positioned for expression of hygromycin phosphotransferase into a host cell and then growing the host cell under selective conditions. The vectors optionally further comprise Cephalosporium ribosomal DNA and also sequences that allow for replication and selection in E. coli and Streptomyces.
摘要翻译：公开了一种转化头孢菌素和其他低等真核生物的方法。该方法包括将含有酿酒酵母转录和翻译活化序列的重组DNA克隆载体插入到宿主细胞中，然后在选择性条件下生长宿主细胞。载体任选地还包含头孢菌核糖体DNA，以及允许在大肠杆菌和链霉菌中复制和选择的序列。

6. 发明授权

US06322998B1 Recombinant DNA compounds that encode ACV synthetase activity of Cephalosporium acremonium 失效
标题翻译：编码顶头孢霉的ACV合成酶活性的重组DNA化合物
公开(公告)号：US06322998B1
公开(公告)日：2001-11-27
申请号：US07968620
申请日：1992-10-29
申请人： Cathleen A. Cantwell , Roland L. Hodges , JoAnn Hoskins , Stephen W. Queener , Paul L. Skatrud
发明人： Cathleen A. Cantwell , Roland L. Hodges , JoAnn Hoskins , Stephen W. Queener , Paul L. Skatrud
IPC分类号： C12P2100
CPC分类号： C12N9/93
摘要： The present invention provides DNA compounds that encode ACV synthetase activity of Cephalosporium acremonium. The compounds can be used to construct recombinant DNA expression vectors for a wide variety of host cells, including E. coli, Penicillium, Cephalosporium, and Aspergillus.
摘要翻译：本发明提供编码顶头孢霉的ACV合成酶活性的DNA化合物。该化合物可用于构建多种宿主细胞的重组DNA表达载体，包括大肠杆菌，青霉，头孢菌和曲霉。

7. 发明授权

US5070020A Recombinant DNA expression vectors and DNA compounds that encode deacetoxycephalosporin C synthetase 失效
标题翻译：重组DNA表达载体和编码脱乙酰壳多糖C合成酶的DNA化合物
公开(公告)号：US5070020A
公开(公告)日：1991-12-03
申请号：US192273
申请日：1988-05-09
申请人： Thomas D. Ingolia , Steven Kovacevic , James R. Miller , Paul L. Skatrud
发明人： Thomas D. Ingolia , Steven Kovacevic , James R. Miller , Paul L. Skatrud
IPC分类号： C12N1/15 , C12N1/21 , C12N9/02 , C12R1/19 , C12R1/465 , C12R1/745 , C12R1/82
CPC分类号： C12N9/0071
摘要： The present invention provides DNA compounds that encode deacetoxycephalosporin C synthetase (DAOCS) activity. The compounds can be used to construct recombinant DNA expression vectors for a wide variety of host cells, including E. coli, Penicillium, Streptomyces, Aspergillus, and Cephalosporium.

8. 发明授权

US5776733A Biosynthetic gene DD1 from Streptococcus pneumoniae 失效
标题翻译：来自肺炎链球菌的生物合成基因DD1
公开(公告)号：US5776733A
公开(公告)日：1998-07-07
申请号：US690413
申请日：1996-07-25
申请人： Paul L. Skatrud , Robert B. Peery
发明人： Paul L. Skatrud , Robert B. Peery
IPC分类号： C12N1/21 , C12N9/00 , C12N15/31 , C12Q1/18 , C07K14/315 , C12N15/63
CPC分类号： C12N9/93 , C12Q1/18
摘要： The invention provides isolated nucleic acid compounds encoding the stem peptide biosynthetic gene ddl of Streptococcus pneumoniae. Also provided are vectors and transformed heterologous host cells for expressing the DDL enzyme product and a method for identifying compounds that inhibit stem peptide biosynthesis.
摘要翻译：本发明提供编码肺炎链球菌的茎肽生物合成基因ddl的分离的核酸化合物。还提供了用于表达DDL酶产物的载体和转化的异源宿主细胞以及用于鉴定抑制干肽生物合成的化合物的方法。

9. 发明授权

US5705352A Multiple drug resistance gene of Aspergillus fumigatus 失效
标题翻译：烟曲霉多重耐药基因
公开(公告)号：US5705352A
公开(公告)日：1998-01-06
申请号：US394880
申请日：1995-02-27
申请人： Robert B. Peery , Paul L. Skatrud
发明人： Robert B. Peery , Paul L. Skatrud
IPC分类号： G01N33/15 , C07K14/38 , C12N1/16 , C12N15/00 , C12N15/09 , C12R1/68 , C12R1/865 , G01N33/53 , G01N33/569
CPC分类号： C07K14/38
摘要： The invention provides isolated nucleic acid compounds encoding a multiple drug resistance protein of Aspergillus fumigatus. Vectors and transformed host cells comprising the multiple drug resistance-encoding DNA of Aspergillus fumigatus MDR-1 are also provided. The invention further provides assays which utilize these transformed host cells.
摘要翻译：本发明提供编码烟曲霉多重耐药性蛋白质的分离的核酸化合物。还提供了包含烟曲霉MDR-1的多重耐药性编码DNA的载体和转化的宿主细胞。本发明进一步提供利用这些转化的宿主细胞的测定法。

10. 发明授权

US5587307A FemA gene of staphylococcus epidermidis, femA protein, and vectors and microorganisms comprising the femA gene 失效
标题翻译： FemA基因，表皮葡萄球菌，femA蛋白，以及包含femA基因的载体和微生物
公开(公告)号：US5587307A
公开(公告)日：1996-12-24
申请号：US330154
申请日：1994-10-27
申请人： William E. Alborn, Jr. , JoAnn Hoskins , Paul L. Skatrud , Serhat Unal
发明人： William E. Alborn, Jr. , JoAnn Hoskins , Paul L. Skatrud , Serhat Unal
IPC分类号： C12N1/21 , C07K14/31 , C12N15/09 , C12N15/31 , C12P21/02 , C12R1/19 , C12R1/44 , C12N15/70
CPC分类号： C07K14/31
摘要： The instant invention provides the femA gene of Staphylococcus epidermidis and all degenerate sequences thereof, the protein encoded by the femA gene (FemA), and vectors and microorganisms comprising genes encoding the FemA protein.
摘要翻译：本发明提供表皮葡萄球菌的femA基因及其所有简并序列，由femA基因（FemA）编码的蛋白质，以及包含编码FemA蛋白的基因的载体和微生物。

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