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1. 发明申请

US20090155869A1 ENGINEERED MICROORGANISMS FOR PRODUCING N-BUTANOL AND RELATED METHODS 审中-公开
标题翻译：用于生产N-丁醇的工程微生物及相关方法
公开(公告)号：US20090155869A1
公开(公告)日：2009-06-18
申请号：US11949724
申请日：2007-12-03
申请人： Thomas Buelter , Andrew C. Hawkins , Kalib Kersh , Peter Meinhold , Matthew W. Peters , Ezhilkani Subbian
发明人： Thomas Buelter , Andrew C. Hawkins , Kalib Kersh , Peter Meinhold , Matthew W. Peters , Ezhilkani Subbian
IPC分类号： C12P7/16 , C12N1/21
CPC分类号： C12N15/52
摘要： A recombinant microorganism expressing at least a heterologous enzyme of an NADH-dependent pathway for conversion of a carbon source to n-butanol, metabolic intermediate and/or a derivative thereof and capable of producing n-butanol, a metabolic intermediate and/or a derivative thereof at a high yield and related methods. The recombinant microorganism engineered to inactivate a native enzyme of one or more pathways that compete with NADH-dependent heterologous pathway, and/or to balance the NADH-dependent heterologous pathway with respect to NADH production and consumption.
摘要翻译：至少表达NADH依赖性途径的异源酶的重组微生物，用于将碳源转化为正丁醇，代谢中间体和/或其衍生物，并能够生产正丁醇，代谢中间体和/或衍生物以高产率和相关方法。重组微生物被工程化以使与NADH依赖性异源途径竞争的一种或多种途径的天然酶失活，和/或平衡NADH依赖性异源途径相对于NADH生产和消耗。

2. 发明申请

US20080293125A1 ENGINEERED MICROORGANISMS FOR PRODUCING ISOPROPANOL 审中-公开
标题翻译：用于生产异丙酚的工程微生物
公开(公告)号：US20080293125A1
公开(公告)日：2008-11-27
申请号：US12106173
申请日：2008-04-18
申请人： Ezhilkani Subbian , Peter Meinhold , Thomas Buelter , Andrew C. Hawkins
发明人： Ezhilkani Subbian , Peter Meinhold , Thomas Buelter , Andrew C. Hawkins
IPC分类号： C07C31/10 , C12N1/21 , C12N1/15 , C12P7/04 , C12N1/19
CPC分类号： C12P7/04
摘要： In an embodiment, there is disclosed a recombinant microbial host cell having each of the DNA molecules encoding a polypeptide or group of polypeptides that catalyze the conversion: (i) Acetyl-CoA to Acetate and CoA (conversion 1) (ii) Acetyl-CoA to Acetoacetyl-CoA and CoA (conversion 2) (iii) Acetoacetyl-CoA and Acetate to Acetoacetate and Acetyl-CoA (conversion 3.1) (iv) Acetoacetate to Acetone and CO2 (conversion 4) (v) Acetone and NAD(P)H and H+ to Isopropanol and NAD(P)+ (conversion 5) wherein at least one DNA molecule is heterologous to the microbial host cell and wherein the microbial host cell produces isopropanol. In another embodiment, a method is disclosed for the production of isopropanol including providing a recombinant microbial host cell, the host cell of (i) with a fermentable carbon substrate in a fermentation medium under conditions whereby isopropanol is produced, and recovering the isopropanol.
摘要翻译：在一个实施方案中，公开了一种重组微生物宿主细胞，其具有编码催化转化的多肽或多肽组的每个DNA分子：<？in-line-formula description =“In-line Formulas”end =“lead” （i）乙酰CoA与乙酸和CoA（转化1）<？在线配方说明=“在线配方”end =“tail”？> <？in-line-formula description =“In- （ii）乙酰CoA至乙酰乙酰辅酶A和CoA（转化2）<？在线公式描述=“内联式”末端=“尾”→> （iii）乙酰乙酰辅酶A和醋酸乙酰乙酸酯和乙酰辅酶A（转化率3.1）<？在线公式描述=“In-line-formula”=“In-line formula”end =“lead” 线式公式“end =”tail“？> <？in-line-formula description =”In-line Formulas“end =”lead“？>（iv）乙酰乙酸酯与丙酮和二氧化碳（转化4）公式description =“In-line Formulas”end =“tail”？> <？in-line-formula description =“In-line Formulas”end =“lead”？>（v）丙酮和NAD （P）H和H +与异丙醇和NAD（P）+（转化5）<？在线公式描述=“内联式”末端=“尾”→其中至少一个DNA分子与微生物宿主细胞，其中微生物宿主细胞产生异丙醇。在另一个实施方案中，公开了用于生产异丙醇的方法，包括提供重组微生物宿主细胞，宿主细胞（i）在发酵培养基中的可发酵碳底物，其中产生异丙醇的条件下，并回收异丙醇。

3. 发明授权

US08431374B2 Methods for the economical production of biofuel from biomass 有权
标题翻译：从生物质经济生产生物燃料的方法
公开(公告)号：US08431374B2
公开(公告)日：2013-04-30
申请号：US12263436
申请日：2008-10-31
申请人： Andrew C. Hawkins , David A. Glassner , Thomas Buelter , James Wade , Peter Meinhold , Matthew W. Peters , Patrick R. Gruber , William A. Evanko , Aristos A. Aristidou , Marco Landwehr
发明人： Andrew C. Hawkins , David A. Glassner , Thomas Buelter , James Wade , Peter Meinhold , Matthew W. Peters , Patrick R. Gruber , William A. Evanko , Aristos A. Aristidou , Marco Landwehr
IPC分类号： C12P7/16 , C12N1/21 , C07H21/04
CPC分类号： C12P7/16 , C07K14/245 , C10G1/04 , C10G2300/1014 , C10G2300/4025 , C10L1/02 , C10L2200/0469 , C10L2290/26 , C12N9/0006 , C12N9/0036 , C12N9/2414 , C12N9/2417 , C12N9/2428 , C12N9/2434 , C12P7/04 , C12P7/24 , C12P7/62 , C12Y106/01002 , C12Y302/01001 , C12Y302/01003 , Y02E50/10 , Y02E50/16 , Y02P30/20
摘要： Methods for producing a biofuel are provided. Also provided are biocatalysts that convert a feedstock to a biofuel.
摘要翻译：提供生产生物燃料的方法。还提供了将原料转化为生物燃料的生物催化剂。

4. 发明申请

US20090226990A1 METHODS FOR THE ECONOMICAL PRODUCTION OF BIOFUEL FROM BIOMASS 有权
标题翻译：从生物量经济生产生物燃料的方法
公开(公告)号：US20090226990A1
公开(公告)日：2009-09-10
申请号：US12263436
申请日：2008-10-31
申请人： Andrew C. Hawkins , David A. Glassner , Thomas Buelter , James Wade , Peter Meinhold , Matthew W. Peters , Patrick R. Gruber , William A. Evanko , Aristos A. Aristidou
发明人： Andrew C. Hawkins , David A. Glassner , Thomas Buelter , James Wade , Peter Meinhold , Matthew W. Peters , Patrick R. Gruber , William A. Evanko , Aristos A. Aristidou
IPC分类号： C12P7/16 , C12N1/21 , C07H21/04
CPC分类号： C12P7/16 , C07K14/245 , C10G1/04 , C10G2300/1014 , C10G2300/4025 , C10L1/02 , C10L2200/0469 , C10L2290/26 , C12N9/0006 , C12N9/0036 , C12N9/2414 , C12N9/2417 , C12N9/2428 , C12N9/2434 , C12P7/04 , C12P7/24 , C12P7/62 , C12Y106/01002 , C12Y302/01001 , C12Y302/01003 , Y02E50/10 , Y02E50/16 , Y02P30/20
摘要： Methods for producing a biofuel are provided. Also provided are biocatalysts that convert a feedstock to a biofuel.
摘要翻译：提供生产生物燃料的方法。还提供了将原料转化为生物燃料的生物催化剂。

5. 发明申请

US20090215137A1 METHODS FOR THE ECONOMICAL PRODUCTION OF BIOFUEL PRECURSOR THAT IS ALSO A BIOFUEL FROM BIOMASS 审中-公开
标题翻译：经济生产生物燃料前体的方法也是生物质的生物燃料
公开(公告)号：US20090215137A1
公开(公告)日：2009-08-27
申请号：US12263442
申请日：2008-10-31
申请人： Andrew C. Hawkins , David A. Glassner , Thomas Buelter , James Wade , Peter Meinhold , Matthew W. Peters , Patrick R. Gruber , William A. Evanko , Aristos A. Aristidou
发明人： Andrew C. Hawkins , David A. Glassner , Thomas Buelter , James Wade , Peter Meinhold , Matthew W. Peters , Patrick R. Gruber , William A. Evanko , Aristos A. Aristidou
IPC分类号： C12P7/16 , C12P1/00 , C12N1/21
CPC分类号： C12P7/16 , C07K14/245 , C10G1/04 , C10G2300/1014 , C10G2300/4025 , C10L1/02 , C10L2200/0469 , C10L2290/26 , C12N9/0006 , C12N9/0036 , C12N9/2414 , C12N9/2417 , C12N9/2428 , C12N9/2434 , C12P7/04 , C12P7/24 , C12P7/62 , C12Y106/01002 , C12Y302/01001 , C12Y302/01003 , Y02E50/10 , Y02E50/16 , Y02P30/20
摘要： Methods for producing a biofuel precursor are provided. Also provided are biocatalysts that convert a feedstock to a biofuel precursor.
摘要翻译：提供生产生物燃料前体的方法。还提供了将原料转化为生物燃料前体的生物催化剂。

6. 发明申请

US20120040080A1 METHODS FOR THE ECONOMICAL PRODUCTION OF BIOFUEL PRECURSOR THAT IS ALSO A BIOFUEL FROM BIOMASS 审中-公开
标题翻译：经济生产生物燃料前体的方法也是生物质的生物燃料
公开(公告)号：US20120040080A1
公开(公告)日：2012-02-16
申请号：US13269457
申请日：2011-10-07
申请人： Andrew C. Hawkins , David A. Glassner , Thomas Buelter , James Wade , Peter Meinhold , Matthew W. Peters , Patrick R. Gruber , William A. Evanko , Aristos A. Aristidou
发明人： Andrew C. Hawkins , David A. Glassner , Thomas Buelter , James Wade , Peter Meinhold , Matthew W. Peters , Patrick R. Gruber , William A. Evanko , Aristos A. Aristidou
IPC分类号： A23K1/06 , C12S3/14 , C12S3/18 , C12S3/00
CPC分类号： C12P7/16 , C07K14/245 , C10G1/04 , C10G2300/1014 , C10G2300/4025 , C10L1/02 , C10L2200/0469 , C10L2290/26 , C12N9/0006 , C12N9/0036 , C12N9/2414 , C12N9/2417 , C12N9/2428 , C12N9/2434 , C12P7/04 , C12P7/24 , C12P7/62 , C12Y106/01002 , C12Y302/01001 , C12Y302/01003 , Y02E50/10 , Y02E50/16 , Y02P30/20
摘要： Methods for producing a biofuel precursor are provided. Also provided are biocatalysts that convert a feedstock to a biofuel precursor.
摘要翻译：提供生产生物燃料前体的方法。还提供了将原料转化为生物燃料前体的生物催化剂。

7. 发明申请

US20120190089A1 ENGINEERED MICROOGRANISMS CAPABLE OF PRODUCING TARGET COMPOUNDS UNDER ANAEROBIC CONDITIONS 审中-公开
标题翻译：在无机条件下生产目标化合物的工程化微生物
公开(公告)号：US20120190089A1
公开(公告)日：2012-07-26
申请号：US13303884
申请日：2011-11-23
申请人： Thomas Buelter , Peter Meinhold , Reid M. Renny Feldman , Andrew C. Hawkins , Sabine Bastian , Frances Arnold , Jun Urano
发明人： Thomas Buelter , Peter Meinhold , Reid M. Renny Feldman , Andrew C. Hawkins , Sabine Bastian , Frances Arnold , Jun Urano
IPC分类号： C12P7/16 , C12N1/19
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N9/0036 , Y02E50/10
摘要： The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.
摘要翻译：本发明通常提供重组微生物，其包含能够在需氧和厌氧条件下生产C3-C5醇的工程化代谢途径。本发明还提供已被突变或修饰以提高其NADH依赖性活性或将辅因子偏好从NADPH转变为NADH并在修饰的微生物中表达的酮酸酸还原异构酶。此外，本发明提供以修饰微生物表达的异丁醛脱氢酶。还提供了在需氧和厌氧条件下通过使合适的底物与本发明的改性微生物接触来生产有益代谢物的方法。

8. 发明授权

US08097440B1 Engineered microorganisms capable of producing target compounds under anaerobic conditions 有权
标题翻译：能够在厌氧条件下产生目标化合物的工程微生物
公开(公告)号：US08097440B1
公开(公告)日：2012-01-17
申请号：US13269937
申请日：2011-10-10
申请人： Thomas Buelter , Peter Meinhold , Reid M. Renny Feldman , Andrew C. Hawkins , Jun Urano , Sabine Bastian , Frances Arnold
发明人： Thomas Buelter , Peter Meinhold , Reid M. Renny Feldman , Andrew C. Hawkins , Jun Urano , Sabine Bastian , Frances Arnold
IPC分类号： C12P7/16 , C12N15/53 , C12N1/15 , C12N9/02
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N9/0036 , Y02E50/10 , Y02E50/17
摘要： The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.
摘要翻译：本发明通常提供重组微生物，其包含能够在需氧和厌氧条件下生产C3-C5醇的工程化代谢途径。本发明还提供已被突变或修饰以提高其NADH依赖性活性或将辅因子偏好从NADPH转变为NADH并在修饰的微生物中表达的酮酸酸还原异构酶。此外，本发明提供以修饰微生物表达的异丁醛脱氢酶。还提供了在需氧和厌氧条件下通过使合适的底物与本发明的改性微生物接触来生产有益代谢物的方法。

9. 发明申请

US20080293101A1 Engineered microorganisms for increasing product yield in biotransformations, related methods and systems 审中-公开
标题翻译：用于提高生物转化，相关方法和系统中产品产量的工程微生物
公开(公告)号：US20080293101A1
公开(公告)日：2008-11-27
申请号：US11881748
申请日：2007-07-27
申请人： Matthew W. Peters , Peter Meinhold , Thomas Buelter , Marco Landwehr
发明人： Matthew W. Peters , Peter Meinhold , Thomas Buelter , Marco Landwehr
IPC分类号： C12P21/04 , C12N1/20 , C12P7/04 , C12M1/00
CPC分类号： C12N9/0036
摘要： There are disclosed recombinant microorganisms engineered to increase product yield in a biotransformation. In an embodiment, the microorganisms are engineered to increase the amount of NAD(P)H available for a NAD(P)H-requiring oxidoreductase involved in a biotransformation. There are also disclosed methods and systems for using recombinant microorganisms engineered to increase the amount of NAD(P)H available for a NAD(P)H-requiring oxidoreductase involved in a biotransformation. Other embodiments are also disclosed.
摘要翻译：公开了在生物转化中改进产物产率的重组微生物。在一个实施方案中，将微生物工程化以增加可用于参与生物转化的NAD（P）H需氧化还原酶的NAD（P）H的量。还公开了使用重组微生物进行工程改造以增加参与生物转化的NAD（P）H需氧化还原酶可用的NAD（P）H量的方法和系统。还公开了其他实施例。

10. 发明申请

US20120045809A1 Engineered Microorganisms for the Production of One or More Target Compounds 审中-公开
标题翻译：用于生产一种或多种靶化合物的工程微生物
公开(公告)号：US20120045809A1
公开(公告)日：2012-02-23
申请号：US13141648
申请日：2009-12-23
申请人： Thomas Buelter , Peter Meinhold , Christopher Smith , Aristos Aristidou , Catherine Asleson Dundon , Jun Urano
发明人： Thomas Buelter , Peter Meinhold , Christopher Smith , Aristos Aristidou , Catherine Asleson Dundon , Jun Urano
IPC分类号： C12P7/16 , C12N1/19
CPC分类号： C12P7/16 , C12N15/52 , C12N15/81 , Y02E50/10
摘要： The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms comprise isobutanol producing metabolic pathway with one or more isobutanol pathway enzymes localized in the mitochondria. In various embodiments described herein, the recombinant microorganisms may be Crabtree-negative yeast microorganisms, microorganisms of the Saccharomyces clade, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译：本发明提供包含产生异丁醇的代谢途径的重组微生物和使用所述重组微生物产生异丁醇的方法。在本发明的各个方面，重组微生物包含产生异丁醇的代谢途径与一种或多种位于线粒体中的异丁醇途径酶。在本文所述的各种实施方案中，重组微生物可以是Crabtree阴性酵母微生物，酵母进化枝的微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物，和非发酵酵母微生物。

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