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1. 发明申请

US20200255879A1 METHODS TO PRODUCE SINGLE GLYCOFORM ANTIBODIES 审中-公开
公开(公告)号：US20200255879A1
公开(公告)日：2020-08-13
申请号：US16736654
申请日：2020-01-07
申请人： The University of Manitoba
发明人： Venkata Tayi , Michael Butler
IPC分类号： C12P21/00 , C07K1/22 , C07K16/00 , C12P19/18 , C07K16/24 , C07K16/28
摘要： An enzymatic method is provided for restructuring an affinity ligand bound heterogenous glycoform antibody sample to a substantially homogenous single desired glycoform antibody sample for therapeutic uses and kits for performing the methods. A method for enzymatically altering the Fc region of an affinity ligand bound antibody from a heterogenous glycoform to a substantially homogenous single glycoform comprises: contacting the affinity ligand bound heterogeneous glycoform antibody with a reaction buffer designed for a particular glycoform modification for a time sufficient and under conditions to modify the glycoform of the Fc region to a substantially homogeneous single form; optionally adding one or more nucleotide sugars and/or cofactors; and releasing the substantially homogeneous single glycoform antibody sample from said affinity ligand. The invention also encompasses biopharmaceuticals comprising single glycoform mAbs and polyclonal sntibodies enzymatically produced for the treatment of cancers and immune disorders as well as compositions comprising the single glycoform antibodies as a biopharmaceutical.

2. 发明授权

US11821017B2 Methods to produce single glycoform antibodies 有权
公开(公告)号：US11821017B2
公开(公告)日：2023-11-21
申请号：US16736654
申请日：2020-01-07
申请人： The University of Manitoba
发明人： Venkata Tayi , Michael Butler
IPC分类号： C12P21/00 , C07K1/22 , C07K16/00 , C12P19/18 , C07K16/24 , C07K16/28
CPC分类号： C12P21/005 , C07K1/22 , C07K16/00 , C07K16/244 , C07K16/2863 , C12P19/18 , C07K2317/24 , C07K2317/41
摘要： An enzymatic method is provided for restructuring an affinity ligand bound heterogenous glycoform antibody sample to a substantially homogenous single desired glycoform antibody sample for therapeutic uses and kits for performing the methods. A method for enzymatically altering the Fc region of an affinity ligand bound antibody from a heterogenous glycoform to a substantially homogenous single glycoform comprises: contacting the affinity ligand bound heterogeneous glycoform antibody with a reaction buffer designed for a particular glycoform modification for a time sufficient and under conditions to modify the glycoform of the Fc region to a substantially homogeneous single form; optionally adding one or more nucleotide sugars and/or cofactors; and releasing the substantially homogeneous single glycoform antibody sample from said affinity ligand. The invention also encompasses biopharmaceuticals comprising single glycoform mAbs and polyclonal antibodies enzymatically produced for the treatment of cancers and immune disorders as well as compositions comprising the single glycoform antibodies as a biopharmaceutical.

3. 发明申请

US20170051328A1 METHODS TO PRODUCE SINGLE GLYCOFORM ANTIBODIES 审中-公开
标题翻译：生产单糖抗体的方法
公开(公告)号：US20170051328A1
公开(公告)日：2017-02-23
申请号：US15118664
申请日：2015-02-18
申请人： The University of Manitoba
发明人： Venkata Tayi , Michael Butler
IPC分类号： C12P21/00 , C07K16/24 , C07K1/22 , C07K16/28
CPC分类号： C12P21/005 , C07K1/22 , C07K16/00 , C07K16/244 , C07K16/2863 , C07K2317/24 , C07K2317/41 , C12P19/18
摘要： An enzymatic method is provided for restructuring an affinity ligand bound heterogenous glycoform antibody sample to a substantially homogenous single desired glycoform antibody sample for therapeutic uses and kits for performing the methods. A method for enzymatically altering the Fc region of an affinity ligand bound antibody from a heterogenous glycoform to a substantially homogenous single glycoform comprises: contacting the affinity ligand bound heterogeneous glycoform antibody with a reaction buffer designed for a particular glycoform modification for a time sufficient and under conditions to modify the glycoform of the Fc region to a substantially homogeneous single form; optionally adding one or more nucleotide sugars and/or cofactors; and releasing the substantially homogeneous single glycoform antibody sample from said affinity ligand. The invention also encompasses biopharmaceuticals comprising single glycoform mAbs and polyclonal antibodies enzymatically produced for the treatment of cancers and immune disorders as well as compositions comprising the single glycoform antibodies as a biopharmaceutical.
摘要翻译：提供了一种酶法，用于将亲和配体结合的异源糖基抗体样品重组为用于治疗用途的基本均匀的单一所需糖形抗体样品和用于进行所述方法的试剂盒。将亲和配体结合的抗体的Fc区酶从酶促改变为基本均匀的单糖形式的方法包括：将亲和配体结合的异源糖基抗体与设计用于特定糖形式修饰的反应缓冲液接触足够的时间将Fc区的糖形式修饰成基本均匀的单一形式的条件; 任选地加入一种或多种核苷酸糖和/或辅因子; 并从所述亲和配体释放基本均匀的单一糖基抗体样品。本发明还包括生物药物，其包含单个糖基单克隆抗体和酶解产生用于治疗癌症和免疫病症的多克隆抗体以及包含单一糖形抗体作为生物药物的组合物。

4. 发明申请

US20150090592A1 SPLIT-SENSOR DIELECTROPHORETIC/MAGNETOPHORETIC CYTOMETER 审中-公开
标题翻译：分体传感器电磁/磁性测微计
公开(公告)号：US20150090592A1
公开(公告)日：2015-04-02
申请号：US14506883
申请日：2014-10-06
申请人： The University of Manitoba
发明人： Douglas Thomson , Gregory Bridges , Michael Butler , Marija Nikolic-Jaric , Elham Salimi , Tim Cabel , Szymon Rzeszowski , Graham Ferrier , Katrin Braasch
IPC分类号： G01N15/10 , G01N27/447
CPC分类号： G01N15/1031 , G01N15/1056 , G01N27/44786 , G01N2015/1006 , G01N2015/1081
摘要： Embodiments of an improved sensor for dielectrophoretic cytometry are presented. In one embodiment, the sensor includes a plurality of sensor electrodes as well as an actuation electrode. Embodiments of microfluidic systems incorporating such sensor are also described. Additionally, embodiments of methods or performing cytometry analysis are also presented.
摘要翻译：提出了一种改进的介电电泳细胞计数传感器的实施例。在一个实施例中，传感器包括多个传感器电极以及致动电极。还描述了结合了这种传感器的微流体系统的实施例。此外，还提出了方法的实施方案或进行细胞分析。

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