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1. 发明申请

WO2021099685A2 NON-VIRAL TRANSCRIPTION ACTIVATION DOMAINS AND METHODS AND USES RELATED THERETO 审中-公开
公开(公告)号：WO2021099685A2
公开(公告)日：2021-05-27
申请号：PCT/FI2020/050772
申请日：2020-11-18
申请人： TEKNOLOGIAN TUTKIMUSKESKUS VTT OY
发明人： MOJZITA, Dominik , KOIVISTOINEN, Outi , SALUMÄE, Astrid
IPC分类号： C12N15/82 , C12N15/80 , C12N15/81 , C07K14/415 , C12N15/85
摘要： The present invention relates to the fields of life sciences, genetics and regulation of gene expression. Specifically, the invention relates to a non-viral transcription activation domain for a eukaryotic host. Also, the present invention relates to a polypeptide or artificial transcription factor comprising the transcription activation domain of the present invention. And furthermore, the present invention relates to a polynucleotide, an expression cassette, expression system, and/or a eukaryotic host. Still, the present invention relates to a method for producing a desired protein product in the eukaryotic host of the present invention or to a method of preparing a non-viral transcription activation domain of the present invention or a polynucleotide encoding said non-viral transcription activation domain. And still further, the present invention relates to use of the transcription activation domain, polypeptide, artificial transcription factor, polynucleotide, expression cassette, expression system or eukaryotic host of the present invention for metabolic engineering and/or production of a desired protein product.

2. 发明申请

WO2019180309A1 HETEROLOGOUS PRODUCTION OF PSILOCYBIN 审中-公开
公开(公告)号：WO2019180309A1
公开(公告)日：2019-09-26
申请号：PCT/FI2019/050199
申请日：2019-03-11
申请人： TEKNOLOGIAN TUTKIMUSKESKUS VTT OY
发明人： MOJZITA, Dominik , RISCHER, Heiko , RANTASALO, Anssi , OKSMAN-CALDENTEY, Kirsi-Marja , KUIVANEN, Joosu
IPC分类号： C12N15/63 , C12N9/10 , C12N15/82
摘要： The invention discloses a recombinant host cell for producing psilocybin and related compounds, such as metabolic intermediates of the psilocybin biosynthesis. Also provided is a method of producing psilocybin and its synthesis intermediates and related compounds, such as metabolic intermediates of the psilocybin biosynthesis, in the host cell, as well as a production system for producing them.

3. 发明申请

WO2017144777A1 EXPRESSION SYSTEM FOR EUKARYOTIC ORGANISMS 审中-公开
标题翻译：真核生物的表达系统
公开(公告)号：WO2017144777A1
公开(公告)日：2017-08-31
申请号：PCT/FI2017/050114
申请日：2017-02-21
申请人： TEKNOLOGIAN TUTKIMUSKESKUS VTT OY
发明人： MOJZITA, Dominik , RANTASALO, Anssi , JÄNTTI, Jussi , LANDOWSKI, Christopher , KUIVANEN, Joosu
IPC分类号： C12N15/79
CPC分类号： C12N15/79
摘要： The present invention provides an expression system for a eukaryotic host, which comprises 1) an expression cassette comprising a core promoter, said core promoter controlling the expression of a DNA sequence encoding a synthetic transcription factor (sTF), and 2) one or more expression cassettes each comprising a DNA sequence encoding a desired product operably linked to a synthetic promoter, said synthetic promoter comprising a core promoter, and sTF-specific binding sites upstream of the core promoter. The present invention also provides a method for identifying universal core promoters for eukaryotic hosts, expression systems using universal core promoters, hosts comprising said systems, and methods for producing protein products in eukaryotic hosts.
摘要翻译：本发明提供了用于真核宿主的表达系统，其包含1）包含核心启动子的表达盒，所述核心启动子控制编码合成转录因子（sTF）的DNA序列的表达，和2）一个或多个表达盒，每个表达盒包含编码与合成启动子可操作连接的所需产物的DNA序列，所述合成启动子包含核心启动子和核心启动子上游的sTF特异性结合位点。本发明还提供了用于鉴定真核宿主的通用核心启动子的方法，使用通用核心启动子的表达系统，包含所述系统的宿主以及用于在真核宿主中产生蛋白质产物的方法。

4. 发明申请

WO2015075316A1 METHOD FOR CULTIVATING YEAST IN LIGNOCELLULOSIC HYDROLYSATE 审中-公开
标题翻译：用于在水杨酸水杨酸酯中培养YEAST的方法
公开(公告)号：WO2015075316A1
公开(公告)日：2015-05-28
申请号：PCT/FI2014/050890
申请日：2014-11-20
申请人： TEKNOLOGIAN TUTKIMUSKESKUS VTT
发明人： NYGÅRD, Yvonne , MOJZITA, Dominik , TOIVARI, Mervi , PENTTILÄ, Merja , RUOHONEN, Laura , WIEBE, Marilyn
IPC分类号： C12N1/19 , C12N1/22 , C07K14/395 , C12P7/10 , C12P7/18
CPC分类号： C12N1/22 , C07K14/395 , C12P7/10 , C12P7/18 , Y02E50/16
摘要： This invention relates to a method for cultivating yeast in lignocellulosic hydrol- ysate and methods of improving yeast viability and/or metabolic activity when cultivated on medium comprising lignocellulosic hydrolysate or in a culture me- dium comprising lignocellulosic hydrolysate or in other formic and acetic acid containing media. The method comprises that yeast is made deficient of a gene encoding the plasma membrane transporter Pdr12 or its homologue. The in- vention relates also to the yeast culture and modified yeast host. The invention improves production of desired products on lignocellulosic or acidic medium.
摘要翻译：本发明涉及一种在木质纤维素水解产物中培养酵母的方法以及在含有木质纤维素水解产物的培养基中或在包含木质纤维素水解产物的培养基中或在其它甲酸和乙酸中培养时改善酵母存活力和/或代谢活性的方法媒体。该方法包括使酵母缺乏编码质膜转运蛋白Pdr12的基因或其同源物。本发明还涉及酵母培养和修饰的酵母宿主。本发明改善木质纤维素或酸性介质中所需产物的生产。

5. 发明公开

EP4061950A2 NON-VIRAL TRANSCRIPTION ACTIVATION DOMAINS AND METHODS AND USES RELATED THERETO 审中-公开
公开(公告)号：EP4061950A2
公开(公告)日：2022-09-28
申请号：EP20816545.6
申请日：2020-11-18
申请人： Teknologian Tutkimuskeskus VTT OY
发明人： MOJZITA, Dominik , KOIVISTOINEN, Outi , SALUMÄE, Astrid
IPC分类号： C12N15/82 , C12N15/80 , C12N15/81 , C07K14/415 , C12N15/85

6. 发明公开

EP3768843A1 HETEROLOGOUS PRODUCTION OF PSILOCYBIN 审中-公开
公开(公告)号：EP3768843A1
公开(公告)日：2021-01-27
申请号：EP19713528.8
申请日：2019-03-11
申请人： Teknologian tutkimuskeskus VTT Oy
发明人： MOJZITA, Dominik , RISCHER, Heiko , RANTASALO, Anssi , OKSMAN-CALDENTEY, Kirsi-Marja , KUIVANEN, Joosu
IPC分类号： C12N15/63 , C12N9/10 , C12N15/82

7. 发明公开

EP3405580A1 EXPRESSION SYSTEM FOR EUKARYOTIC ORGANISMS 有权转让
公开(公告)号：EP3405580A1
公开(公告)日：2018-11-28
申请号：EP17712530.9
申请日：2017-02-21
申请人： Teknologian Tutkimuskeskus VTT Oy
发明人： MOJZITA, Dominik , RANTASALO, Anssi , JÄNTTI, Jussi , LANDOWSKI, Christopher , KUIVANEN, Joosu
IPC分类号： C12N15/79
CPC分类号： C12N15/79
摘要： The present invention provides an expression system for a eukaryotic host, which comprises 1) an expression cassette comprising a core promoter, said core promoter controlling the expression of a DNA sequence encoding a synthetic transcription factor (sTF), and 2) one or more expression cassettes each comprising a DNA sequence encoding a desired product operably linked to a synthetic promoter, said synthetic promoter comprising a core promoter, and sTF-specific binding sites upstream of the core promoter. The present invention also provides a method for identifying universal core promoters for eukaryotic hosts, expression systems using universal core promoters, hosts comprising said systems, and methods for producing protein products in eukaryotic hosts.

8. 发明公开

EP2408925A1 MANUFACTURE OF XYLONIC ACID 审中-公开
标题翻译：制造木糖
公开(公告)号：EP2408925A1
公开(公告)日：2012-01-25
申请号：EP10753171.7
申请日：2010-03-17
申请人： Teknologian Tutkimuskeskus VTT
发明人： RICHARD, Peter , WIEBE, Marilyn , TOIVARI, Mervi , MOJZITA, Dominik , RUOHONEN, Laura , PENTTILÄ, Merja
IPC分类号： C12P7/58 , C12N1/15 , C12N1/19 , C12N9/04 , C12N15/53
CPC分类号： C12P7/58 , C12N9/0006
摘要： The invention provides a method for producing xylonic acid from xylose with a recombinant fungal strain that is genetically modified to express a xylose dehydrogenase gene, which is able to convert xylose to xylonolactone, which is spontaneously or enzymatically hydrolysed to xylonic acid. The xylonic acid is excreted outside the host cell. Xylonate production may be coupled with xylitol production. Alternatively, if xylitol production is not desired, its production is reduced by removing the aldose reductase (or specific xylose reductase) enzyme, which converts xylose to xylitol. Expression of a heterologous lactonase encoding gene may result in higher acid concentrations. The method is especially suitable for producing xylonic acid from a hemicelluiose hydrolysate such as hydrolysed lignocellulosic plant biomass.

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