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1. 发明授权

US5206170A Sulfur dioxide detector device embodied by using S02-oxidant bioagent 失效
标题翻译：通过使用SO2-氧化物生物体实现的硫二氧化物检测器件
公开(公告)号：US5206170A
公开(公告)日：1993-04-27
申请号：US769534
申请日：1991-10-01
申请人： Takeshi Sato , Nobuko Kubo , Hirofumi Akano , Yoshiya Kawamura , Shigesada Iijima
发明人： Takeshi Sato , Nobuko Kubo , Hirofumi Akano , Yoshiya Kawamura , Shigesada Iijima
IPC分类号： C12Q1/00 , G01N33/00
CPC分类号： C12Q1/005 , C12Q1/001 , G01N33/0042 , Y10S435/817
摘要： In a sulfur dioxide detector using SO.sub.2 -oxidant microbes, a bio-membrane is located at one end of an oxygen concentration sensor, the bio-membrane containing the SO.sub.2 -oxidant microbes between an immobilized membrane and a gas permeable membrane. The sensor has an electrode in contact with the immobilized membrane. A dish-shaped or frusto-conical cell has an open end which is in contact with the gas permeable membrane so that SO.sub.2 -laden solution supplied to the cell causes sulfurous acid to oxidize to sulfuric acid as the SO.sub.2 permeates through the bio-membrane so as to change an output from the sensor. An inlet and outlet hole are formed on a sidewall of the cell. The outlet hole is located to be always directly above the inlet hole so as to drain foam formed when the SO.sub.2 -laden solution is supplied to the cell through the inlet hole and drained from the outlet hole.

2. 发明授权

US5909114A Coulometric analysis method and a device therefor 失效
标题翻译：库仑分析法及其设备
公开(公告)号：US5909114A
公开(公告)日：1999-06-01
申请号：US957393
申请日：1997-10-23
申请人： Shunichi Uchiyama , Takeshi Sato , Hirofumi Akano , Yoshiya Kawamura
发明人： Shunichi Uchiyama , Takeshi Sato , Hirofumi Akano , Yoshiya Kawamura
IPC分类号： G01N27/42 , G01N33/18 , G01N27/26
CPC分类号： G01N27/423 , G01N33/1806
摘要： The present invention provides a rapid coulometric analysis for the quantitative determination of a sample substance with high reproducibility without any application of voltage etc. from the outside, as well as a galvanic cell and a device for use in said coulometric analysis. According to the present invention, a wide variety of components can be analyzed by selection of electroactive substance introduced into the galvanic cell. The present invention enables a simple and easy analysis for food components such as glutamic acid, ascorbic acid, etc., water quality such as COD, etc.
摘要翻译：本发明提供了一种用于定量测定样品物质的快速库仑分析，其重现性高，而没有外部施加电压等，以及用于所述库仑分析的电池和装置。根据本发明，可以通过选择引入到原电池中的电活性物质来分析各种各样的组分。本发明能够简单易用地分析谷物酸，抗坏血酸等食品成分，COD等水质等。

3. 发明授权

US5085851A Dental plaque-degrading compositions 失效
标题翻译：牙膏降解组合物
公开(公告)号：US5085851A
公开(公告)日：1992-02-04
申请号：US552533
申请日：1990-07-16
申请人： Gentaro Okada , Hirofumi Akano , Takeshi Sato , Hajime Okumura , Yoshiya Kawamura
发明人： Gentaro Okada , Hirofumi Akano , Takeshi Sato , Hajime Okumura , Yoshiya Kawamura
IPC分类号： A61K8/66 , A61K38/46 , A61P1/02 , A61Q11/00
CPC分类号： A61K8/66 , A61Q11/00 , Y10S514/90 , Y10S514/901 , Y10S514/902
摘要： A dental plaque-degrading composition containing endodextranase produced by Arthrobacter globiformis W31 exhibiting high degradation efficacy to insoluble glucan produced by Streptococcus mutans IFO 13955, the dental plaque-degrading composition additionally containing .alpha.-amylase of various origins. These compositions are useful for preventing the settlement of Streptococcus mutans in the oral cavity and the formation of new dental plaque and for preventing the dental caries, and provided in the form of dentifrices, denture detergents, troches, mouth washes, chewing gums or candies.

4. 发明授权

US5426042A Klebsella oxytoca ferm BP-I/3616 and immobilization thereof with a gelating agent in pores of a membrane 失效
标题翻译： Klebsella oxytoca ferm BP-I / 3616，并用胶凝剂在膜的孔中固定
公开(公告)号：US5426042A
公开(公告)日：1995-06-20
申请号：US179593
申请日：1994-01-07
申请人： Shigeru Maeda , Akira Ohki , Takeshi Sato , Naho Kato , Hirofumi Akano , Yoshiya Kawamura , Keizo Hatagaki , Yasushi Takahashi , Mikio Yamada , Hajime Okumura
发明人： Shigeru Maeda , Akira Ohki , Takeshi Sato , Naho Kato , Hirofumi Akano , Yoshiya Kawamura , Keizo Hatagaki , Yasushi Takahashi , Mikio Yamada , Hajime Okumura
IPC分类号： C12Q1/00 , G01N33/18 , C12N11/10 , C12N1/12 , C12N11/04 , G01N27/26
CPC分类号： G01N33/1806 , C12Q1/001 , G01N33/1866 , G01N33/1893 , Y10S435/817 , Y10S435/852
摘要： A BOD analyzer is prepared having a microbe sensor containing an oxygen electrode and a microbe membrane. The microbe membrane is made by immobilizing microorganisms belonging to the genus Klebsiella in the membrane. Specifically, the BOD analyzer has a flow cell equipped with a microbe sensor containing an oxygen electrode and a microbe membrane, and a liquid passage which is connected with an entrance of the flow cell and which is equipped with an outlet. The microbe membrane is made by immobilizing microorganisms belonging to Klebsiella oxytoca 12092 strain in a porous hydrophilic membrane having an average pore size of 0.65-3 .mu.m in diameter by using at least one gelating agent selected from alginic acid or salts thereof, agar, gellan gum, xathane gum, gelatine, carageenan, locust bean gum, methylcellulose, pectin, or pullulan. The BOD analyzer can be used for batch or continuous BOD analysis and enables carrying out BOD analysis in a short period of time.
摘要翻译：制备具有含有氧电极和微生物膜的微生物传感器的BOD分析仪。微生物膜通过将属于克雷伯菌属的微生物固定在膜中而制成。具体而言，BOD分析装置具有配置有含有氧电极和微生物膜的微生物传感器的流通池，与流通池的入口连接并具有出口的液体通道。通过使用至少一种选自藻酸或其盐，琼脂，结冷胶的凝胶剂，将属于克雷伯氏菌12092菌株的微生物直接固定在平均孔径为0.65-3μm的多孔亲水膜中来制备微生物膜口香糖，xathane胶，明胶，角叉菜胶，刺槐豆胶，甲基纤维素，果胶或支链淀粉。 BOD分析仪可用于批量或连续BOD分析，可在短时间内进行BOD分析。

5. 发明授权

US5726565A Coulometric analysis method and a device therefor 失效
标题翻译：库仑分析法及其设备
公开(公告)号：US5726565A
公开(公告)日：1998-03-10
申请号：US511476
申请日：1995-08-04
申请人： Shunichi Uchiyama , Takeshi Sato , Hirofumi Akano , Yoshiya Kawamura , Masahiro Fukaya , Hiroaki Ebisuya , Ko Furukawa , Kazuyo Kajino , Sumio Akita
发明人： Shunichi Uchiyama , Takeshi Sato , Hirofumi Akano , Yoshiya Kawamura , Masahiro Fukaya , Hiroaki Ebisuya , Ko Furukawa , Kazuyo Kajino , Sumio Akita
IPC分类号： G01N27/42 , G01N33/18 , G01N27/26
CPC分类号： G01N27/423 , G01N33/1806
摘要： The present invention provides a rapid coulometric analysis for the quantitative determination of a sample substance wherein the coulometric analysis has high reproducibility without any application of voltage, etc., from an outside source, as well as a galvanic cell and a device for use in said coulometric analysis. According to the present invention, a wide variety of components can be analyzed by selection of electroactive substance introduced into the galvanic cell. The present invention enables a simple and easy analysis for food components such as glutamic acid, ascorbic acid, etc., water quality such as COD, etc.
摘要翻译：本发明提供了用于定量测定样品物质的快速库仑分析，其中库仑分析具有高的再现性，而不需要外部来源的电压等的施加，以及用于所述的电池的电池和装置库仑分析根据本发明，可以通过选择引入到原电池中的电活性物质来分析各种各样的组分。本发明能够简单易用地分析谷物酸，抗坏血酸等食品成分，COD等水质等。

6. 发明授权

US5385830A Apparatus for measuring free and total sulfurous acid and method of measurement 失效
标题翻译：用于测量游离和总亚硫酸的设备和测量方法
公开(公告)号：US5385830A
公开(公告)日：1995-01-31
申请号：US771632
申请日：1991-10-04
申请人： Yoshifumi Amano , Kazuo Nakamura , Hiroshi Kurosawa , Takeshi Sato , Hirofumi Akano , Yoshiya Kawamura
发明人： Yoshifumi Amano , Kazuo Nakamura , Hiroshi Kurosawa , Takeshi Sato , Hirofumi Akano , Yoshiya Kawamura
IPC分类号： C12Q1/00 , C12Q1/02 , C12Q1/26 , C12Q1/32 , C12S3/00 , C12M1/00 , C12P1/04 , C12P11/00
CPC分类号： C12Q1/02 , C12Q1/001 , C12Q1/26 , C12Q1/32 , Y10T436/15 , Y10T436/18 , Y10T436/184 , Y10T436/186 , Y10T436/188
摘要： The present invention provides a method for measuring the amount of free sulfurous acid and bound sulfurous acid in a sample using a bacterium belonging to Thiobacillus thiooxidans or Thiobacillus ferrooxidans of in which the sample is treated with acid and/or alkali to give free sulfurous acid alone in the sample. Then, the total amount of free sulfurous acid in the sample is measured by an oxidation reaction of free sulfurous acid to sulfuric acid using a bacterium belonging to Thiobacillus thiooxidans or Thiobacillus ferrooxidans.
摘要翻译：本发明提供了使用属于硫氧化硫杆菌（Thiobacillus thiooxidans）或硫氧化硫杆菌（Thiobacillus ferrooxidans）的细菌测量样品中游离亚硫酸和结合亚硫酸的量的方法，其中样品用酸和/或碱处理以产生单独的亚硫酸在样品中。然后，使用属于硫氧化硫杆菌（Thiobacillus thiooxidans）或硫氧化硫杆菌（Thiobacillus ferrooxidans）的细菌，通过游离亚硫酸与硫酸的氧化反应测定样品中游离亚硫酸的总量。

7. 发明授权

US5356792A Biochemical oxygen demand analyzer and methods of analysis using Klebsiella microorganisms 失效
标题翻译：生化需氧量分析仪和使用克雷伯菌微生物分析方法
公开(公告)号：US5356792A
公开(公告)日：1994-10-18
申请号：US978843
申请日：1992-11-19
申请人： Shigeru Maeda , Akira Ohki , Takeshi Sato , Naho Kato , Hirofumi Akano , Yoshiya Kawamura , Hatagaki Keizo , Yasushi Takahashi , Mikio Yamada , Hajime Okumura
发明人： Shigeru Maeda , Akira Ohki , Takeshi Sato , Naho Kato , Hirofumi Akano , Yoshiya Kawamura , Hatagaki Keizo , Yasushi Takahashi , Mikio Yamada , Hajime Okumura
IPC分类号： C12Q1/00 , G01N33/18 , C12Q1/24 , G01N27/40
CPC分类号： G01N33/1806 , C12Q1/001 , G01N33/1866 , G01N33/1893 , Y10S435/817 , Y10S435/852
摘要： The present invention relates to a BOD analyzer comprising a microbe sensor containing an oxygen electrode and a microbe membrane. The microbe membrane is made by immobilizing microorganisms belonging to the genus Klebsiella in membrane. Specifically, the present invention relates to a BOD analyzer comprising flow cell equipped with a microbe sensor containing an oxygen electrode and a microbe membrane; and a liquid passage which is connected with the entrance of the flow cell and which is equipped with an outlet. The microbe membrane is made by immobilizing microorganisms belonging to Klebsiella oxytoca 12092 strain in a porous hydrophilic membrane having an average pore size of 0.65-3 .mu.m in diameter by using at least one gelating agent selected from the group consisting of alginic acid or salts thereof, agar, gellan gum, xanthane gum, gelatine, carageenan, locust bean gum, methylcellulose, pectin, and pullulan. The present invention also relates to improved methods of analyzing BOD by using the BOD analyzer.
摘要翻译：本发明涉及一种包含含有氧电极和微生物膜的微生物传感器的BOD分析仪。微生物膜通过将属于克雷伯菌属的微生物固定在膜中而制成。具体而言，本发明涉及一种BOD分析装置，其特征在于，包括配置有含有氧电极和微生物膜的微生物传感器的流动池，以及与流通池的入口连接且配有出口的液体通道。通过使用至少一种选自藻酸或其盐的凝胶剂，将属于克雷伯氏菌12092菌株的微生物固定在直径为0.65-3μm平均孔径的多孔亲水膜中来制备微生物膜，琼脂，结冷胶，黄原胶，明胶，角叉菜胶，刺槐豆胶，甲基纤维素，果胶和支链淀粉。本发明还涉及使用BOD分析仪分析BOD的改进方法。

8. 发明授权

US4996064A Novel foodstuff from soymilk and method for production thereof 失效
标题翻译：来自豆浆的新型食品及其生产方法
公开(公告)号：US4996064A
公开(公告)日：1991-02-26
申请号：US396662
申请日：1989-08-22
申请人： Hirofumi Akano , Takeshi Sato , Hajime Okumura , Yoshiya Kawamura , Kyo Shimada
发明人： Hirofumi Akano , Takeshi Sato , Hajime Okumura , Yoshiya Kawamura , Kyo Shimada
IPC分类号： A23C20/02 , A23L11/00
CPC分类号： A23C20/025 , A23L11/09
摘要： A novel foodstuff is produced by reacting a milk-coagulating enzyme which is produced by a milk-coagulating enzyme-producing microorganism belonging to genus Aspergillus, genus Mucor, or genus Rhizopus and which exhibits a milk-coagulating activity (A) and a protease activity (B) such that the ratio of (A)/(B) is larger than 0.1, with soymilk thereby inducing coagulation, and collecting a resulting coagulated material. The novel foodstuffs produced have the benefit of lacking the bitterness and astringency common to soybean, abounds in emulsifying activity, exhibit a high water-retention property, and are smoothly agreeable to the taste. Thus, they have extensive utility as substitutes for raw materials in conventional processed foods. They are especially suitable for use as raw materials for emulsifiable foods.
摘要翻译：通过使属于曲霉属，毛霉属或根霉属的乳凝固酶生成微生物产生的乳化凝固酶与乳固醇活性（A）和蛋白酶活性（A）反应而制造的新型食品（B）使得（A）/（B）的比例大于0.1，用豆浆从而引起凝结，并收集所得的凝固材料。所生产的新型食品具有缺乏大豆常见的苦味和涩味的优点，乳化活性大，显示出高保水性，并且味道顺利。因此，它们作为常规加工食品中原料的替代物具有广泛的用途。它们特别适合用作可乳化食品的原料。

9. 发明授权

US5340461A Electrophoretic medium for electrophoretic separation, gel holder for holding the same medium, slab type electrophoretic apparatus using the same medium and gel holder, and electrophoretic gel cutter 失效
标题翻译：用于电泳分离的电泳介质，用于保持相同介质的凝胶保持器，使用相同介质和凝胶保持器的板式电泳装置，以及电泳凝胶切割器
公开(公告)号：US5340461A
公开(公告)日：1994-08-23
申请号：US12194
申请日：1993-02-02
申请人： Tomoaki Fukushige , Hiroko Toda , Hirofumi Akano , Emiko Ito , Tomohiko Fukuta , Masahiro Fujimori , Yoshiya Kawamura
发明人： Tomoaki Fukushige , Hiroko Toda , Hirofumi Akano , Emiko Ito , Tomohiko Fukuta , Masahiro Fujimori , Yoshiya Kawamura
IPC分类号： B01D57/02 , G01N27/447 , C25B9/00
CPC分类号： G01N27/44704 , B01D57/02 , G01N27/44747
摘要： An electrophoretic medium best suited as a separation medium is composed of a gel solution and contains a water-soluble polymer and permits great improvement of the enzymic protein recovery character. A gel holder is provided with stopper members and thus can hold even a soft or slippery while maintaining a fixed shape thereof, thus permitting reliable separation and purification of an intended substance without possibility of denaturing of the substance. A slab type electrophoretic apparatus permits automatic detection of the electrophoretic state of a sample and automatic completion of the electrophoresis under optimum conditions without setting measurement condition, permits continuous measurement of the buffer solution temperature, which is important for obtaining an electrophoretic condition for maintaining the enzymic function, and permits separation and purification of a large quantity of an enzyme or like physiologically active substance in a very simple operation. An electrophoretic gel cutter cuts even large size electrophoretic gels vary accurately and safely to desired sizes and particularly cuts a soft and readily deformable electrophoretic gel of polyacrylamide or the like in a pressurized state, thus permitting accurate and safe cutting of the polyacrylamide gel, which has been very difficult to cut, without causing damage to the gel.
摘要翻译：最适合作为分离介质的电泳介质由凝胶溶液组成并含有水溶性聚合物，并且可大大提高酶蛋白质的恢复特性。凝胶保持器设置有止动件，因此可以保持柔软或滑动，同时保持其固定形状，从而允许可预期的物质的可靠分离和纯化，而不会使物质变性。板式电泳装置允许自动检测样品的电泳状态，并在最佳条件下自动完成电泳，而不设置测量条件，允许连续测量缓冲溶液温度，这对于获得维持酶的电泳条件是重要的功能，并且允许在非常简单的操作中分离和纯化大量的酶或类似的生理活性物质。电泳凝胶切割机甚至将大尺寸电泳凝胶切割成精确和安全地变化到所需尺寸，并且特别地在加压状态下切割聚丙烯酰胺等的柔软且容易变形的电泳凝胶，从而允许精确和安全地切割聚丙烯酰胺凝胶，其具有非常难以切割，不会对凝胶造成伤害。

10. 发明授权

US5679572A Separation of chiral compounds on polysaccharide supports 失效
标题翻译：手性化合物在多糖载体上的分离
公开(公告)号：US5679572A
公开(公告)日：1997-10-21
申请号：US239698
申请日：1994-05-09
申请人： Yoshio Okamoto , Naoki Enomoto , Ryu Ohishi , Yasushi Ogasawara , Hirofumi Akano , Yoshiya Kawamura
发明人： Yoshio Okamoto , Naoki Enomoto , Ryu Ohishi , Yasushi Ogasawara , Hirofumi Akano , Yoshiya Kawamura
IPC分类号： B01J20/32 , C07B57/00 , C08B33/00 , C08B37/00 , B01D15/08 , C01B33/12 , G01N30/48
CPC分类号： B01J20/3219 , B01J20/286 , B01J20/289 , B01J20/29 , B01J20/3274 , C07B57/00 , C08B33/00 , C08B37/00 , Y10S435/803
摘要： Provided is a novel polysaccharide derivative having a main structure of the following formula (1) in which a polysaccharide or its derivative has been chemically bonded to the inner and outer surfaces of the pores of a porous carrier at the reducing terminal of the polysaccharide or polysaccharide derivative: ##STR1## Also provided are a method of producing the novel polysaccharide derivative in which a silane agent is chemically bonded to a porous carrier and thereafter a polysaccharide having a degree of polymerization from 11 to 500 or its derivative is further chemically bonded to surface treated carrier at the reducing terminal of the polysaccharide or its derivative, and a method of producing the novel polysaccharide derivative in which a polysaccharide having a degree of polymerization from 11 to 500 or its derivative is chemically bonded to a silane agent at the reducing terminal of the polysaccharide or its derivative and thereafter the polysaccharide derivative is further chemically bonded to a porous carrier. Further provided is a separating agent for chromatography containing the novel polysaccharide derivative. The novel polysaccharide derivative has excellent solvent resistance and is useful as a separating agent for chiral resolution of chiral compounds by chromatography. The methods of the present invention efficiently give the novel polysaccharide derivative.
摘要翻译：本发明提供一种具有下述通式（1）的主要结构的新型多糖衍生物，其中多糖或其衍生物在多糖或多糖的还原端已经与多孔载体的孔的内表面和外表面化学键合衍生物：（1）+ TR 还提供了一种制备新型多糖衍生物的方法，其中硅烷试剂与多孔载体化学键合，然后是聚合度为11〜500的多糖或其衍生物在多糖或其衍生物的还原末端进一步化学键合到表面处理的载体上，以及制备其聚合度为11〜500的多糖或其衍生物与其衍生物化学键合的新型多糖衍生物的方法多糖或其衍生物的还原末端的硅烷试剂，之后是多糖衍生物 e进一步化学键合到多孔载体上。还提供了含有新型多糖衍生物的色谱分离剂。该新型多糖衍生物具有优异的耐溶剂性，可用作通过色谱手性拆分手性化合物的分离剂。本发明的方法有效地提供了新型多糖衍生物。

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