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1. 发明授权

US5496719A Polypeptide from Humicola insolens possessing protein disulfide isomerase activity gene encoding the same 失效
标题翻译：来自Humicola insolens的多肽具有编码相同的蛋白质二硫键异构酶活性基因
公开(公告)号：US5496719A
公开(公告)日：1996-03-05
申请号：US68395
申请日：1993-05-27
申请人： Yukio Yamada , Osamu Asami , Hidehiko Sugiyama , Chie Idekoba , Fumihiko Hoshino , Masana Hirai , Tsutomu Kajino , Takao Imaeda , Kiyoko Sarai
发明人： Yukio Yamada , Osamu Asami , Hidehiko Sugiyama , Chie Idekoba , Fumihiko Hoshino , Masana Hirai , Tsutomu Kajino , Takao Imaeda , Kiyoko Sarai
IPC分类号： C12N9/90 , A61K38/00 , C07K1/00 , C12N9/00
CPC分类号： C12N9/90
摘要： A highly thermostable polypeptide possessing protein disulfide isomerase (PDI) activity, a gene coding for the polypeptide and a process for producing the polypeptide are provided. The polypeptide possessing PDI activity is characterized by A) having a capability of catalyzing a disulfide exchange in proteins, B) recognizing mainly ribonuclease A as a substrate, C) having a suitable active temperature of 20.degree. to 70.degree. C., D) being stable at a pH value of 6 to 9, and E) having a molecular weight of about 60,000 to 62,000. Since it has a higher thermostability and exhibits a stable activity in a wider dithiothreitol concentration range as compared with the conventional PDI, it is possible to provide a novel enzyme active protein which can be advantageously used for a refolding reaction of certain proteins. Further, a process which enables the polypeptide possessing PDI activity to be efficiently produced using Humicola insolens or a transformant transformed with an expression vector containing the above-described gene is also provided.
摘要翻译：提供了具有蛋白质二硫键异构酶（PDI）活性的高度热稳定的多肽，编码多肽的基因和产生多肽的方法。具有PDI活性的多肽的特征在于：A）具有催化蛋白质中二硫键交换的能力，B）主要以核糖核酸酶A为底物，C）具有20〜70℃的合适的活性温度，D）为在pH值为6〜9的条件下稳定，E）分子量约为60,000〜62,000。由于与常规PDI相比，具有较高的热稳定性并且在更宽的二硫苏糖醇浓度范围内表现出稳定的活性，因此可以提供可有利地用于某些蛋白质的重折叠反应的新型酶活性蛋白质。此外，还提供了能够使用Humicola insolens或使用含有上述基因的表达载体转化的转化体有效地产生具有PDI活性的多肽的方法。

2. 发明申请

US20050214915A1 Method of controlling ethanol production and mass production of lactic acid and transformant therefor 失效
标题翻译：控制乙醇生产和大量生产乳酸及其转化体的方法
公开(公告)号：US20050214915A1
公开(公告)日：2005-09-29
申请号：US10507129
申请日：2003-03-11
申请人： Satoshi Saito , Osamu Saotome , Noriko Yasutani , Yasuo Matsuo , Nobuhiro Ishida , Masana Hirai , Takao Imaeda , Chikara Miyazaki , Kenro Tokuhiro
发明人： Satoshi Saito , Osamu Saotome , Noriko Yasutani , Yasuo Matsuo , Nobuhiro Ishida , Masana Hirai , Takao Imaeda , Chikara Miyazaki , Kenro Tokuhiro
IPC分类号： C12N1/15 , C12N9/04 , C12N15/53 , C12P7/56 , C12P7/40 , C12N1/18 , C12N15/74
CPC分类号： C12N9/0006 , C12P7/56 , C12Y101/01027
摘要： The present invention provides a transformant into which has been incorporated DNA for coding a foreign protein having lactate dehydrogenase activity and provided with pyruvic acid substrate affinity that equals or exceeds the pyruvic acid substrate affinity of the pyruvate decarboxylase inherent in the host organism. Said transformant can stably mass-produce lactic acid inside a host organism having the pyruvate decarboxylase gene.
摘要翻译：本发明提供了一种转化体，其中掺入了用于编码具有乳酸脱氢酶活性的外源蛋白质的DNA并且具有等于或超过固有在宿主生物体中的丙酮酸脱羧酶的丙酮酸底物亲和力的丙酮酸底物亲和力的DNA。所述转化体可以在具有丙酮酸脱羧酶基因的宿主生物体内稳定地大量生产乳酸。

3. 发明授权

US08039238B2 Method of controlling ethanol production and mass production of lactic acid and transformant therefor 失效
标题翻译：控制乙醇生产和大量生产乳酸及其转化体的方法
公开(公告)号：US08039238B2
公开(公告)日：2011-10-18
申请号：US10507129
申请日：2003-03-11
申请人： Satoshi Saito , Osamu Saotome , Noriko Yasutani , Yasuo Matsuo , Nobuhiro Ishida , Masana Hirai , Takao Imaeda , Chikara Miyazaki , Kenro Tokuhiro
发明人： Satoshi Saito , Osamu Saotome , Noriko Yasutani , Yasuo Matsuo , Nobuhiro Ishida , Masana Hirai , Takao Imaeda , Chikara Miyazaki , Kenro Tokuhiro
IPC分类号： C12P7/40 , C12N1/18 , C12N15/74
CPC分类号： C12N9/0006 , C12P7/56 , C12Y101/01027
摘要： The present invention provides a transformant into which has been incorporated DNA for coding a foreign protein having lactate dehydrogenase activity and provided with pyruvic acid substrate affinity that equals or exceeds the pyruvic acid substrate affinity of the pyruvate decarboxylase inherent in the host organism. Said transformant can stably mass-produce lactic acid inside a host organism having the pyruvate decarboxylase gene.
摘要翻译：本发明提供了一种转化体，其中掺入了用于编码具有乳酸脱氢酶活性的外源蛋白质的DNA并且具有等于或超过固有在宿主生物体中的丙酮酸脱羧酶的丙酮酸底物亲和力的丙酮酸底物亲和力的DNA。所述转化体可以在具有丙酮酸脱羧酶基因的宿主生物体内稳定地大量生产乳酸。

4. 发明授权

US5702883A Methods for detection of mutagens using luminescence gene 失效
标题翻译：使用发光基因检测诱变剂的方法
公开(公告)号：US5702883A
公开(公告)日：1997-12-30
申请号：US326949
申请日：1994-10-21
申请人： Takao Imaeda , Masana Hirai
发明人： Takao Imaeda , Masana Hirai
IPC分类号： C07K14/47 , C12N15/53 , C12Q1/02 , C12Q1/68 , C07H21/04 , C12Q1/66
CPC分类号： C07K14/47 , C12Q1/025 , C12Q1/6897
摘要： A method for detecting or quantitating a mutagenic substance in a sample includes culturing a host microorganism transformed with a recombinant gene comprising an SOS gene and genes expressing luciferase activity and optionally genes expressing an enzyme which catalyzes the production of a substrate for luciferase, positioned downstream of the SOS gene, in a medium to which the sample is added; and measuring a luminescence generated by expression of the gene expressing luciferase activity. The method is sensitive, accurate and non-time consuming; and gene systems used for said method, i.e., a recombinant gene comprising an SOS gene expressed when a DNA is damaged and a gene expressing luciferase activity positioned downstream of the SOS gene, and a host microorganism transformed with said recombinant gene. Preferably the recombinant gene further comprises a gene expressing an enzyme which catalyses the production of a substrate for the luciferase in the down stream of the SOS gene.
摘要翻译：用于检测或定量样品中的致突变物质的方法包括培养用包含SOS基因的重组基因和表达荧光素酶活性的基因转化的宿主微生物，以及任选地表达催化产生荧光素酶底物的酶的基因，所述基因位于下游在添加样品的培养基中的SOS基因; 并测定通过表达荧光素酶活性的基因的表达产生的发光。该方法灵敏，准确，无耗时; 以及用于所述方法的基因系统，即包含DNA受损时表达的SOS基因的重组基因和位于SOS基因下游的表达荧光素酶活性的基因以及用所述重组基因转化的宿主微生物。优选地，重组基因还包含表达在SOS基因的下游中催化萤光素酶底物的产生的基因的基因。

5. 发明授权

US5700659A Polypeptide possessing protein disulfide isomerase activity gene encoding the same and process for producing the same 失效
标题翻译：具有编码相同蛋白质二硫键异构酶活性基因的多肽及其制备方法
公开(公告)号：US5700659A
公开(公告)日：1997-12-23
申请号：US464365
申请日：1995-06-05
申请人： Yukio Yamada , Osamu Asami , Hidehiko Sugiyama , Chie Idekoba , Fumihiko Hoshino , Masana Hirai , Tsutomu Kajino , Takao Imaeda , Kiyoko Sarai
发明人： Yukio Yamada , Osamu Asami , Hidehiko Sugiyama , Chie Idekoba , Fumihiko Hoshino , Masana Hirai , Tsutomu Kajino , Takao Imaeda , Kiyoko Sarai
IPC分类号： C12N9/90 , C12N15/52 , C12N15/31
CPC分类号： C12N9/90
摘要： A highly thermostable polypeptide possessing protein disulfide isomerase (PDI) activity, a gene coding for the polypeptide and a process for producing the polyeptide are provided. The polypeptide possessing PDI activity is characterized by A) having a capability of catalyzing a disulfide exchange in proteins, B) recognizing mainly ribonuclease A as a substrate, C) having a suitable active temperature of 20.degree. to 70.degree. C., D) being stable at a pH value of 6 to 9, and E) having a molecular weight of about 60,000 to 62,000. Since it has a higher thermostability and exhibits a stable activity in a wider dithiothreitol concentration range as compared with the conventional PDI, it is possible to provide a novel enzyme active protein which can be advantageously used for a refolding reaction of certain proteins. Further, a process which enables the polylpeptide possessing PDI activity to be efficiently produced using Humicola insolens or a transformant transformed with an expression vector containing the above-described gene is also provided.
摘要翻译：提供了具有蛋白质二硫键异构酶（PDI）活性的高度热稳定的多肽，编码多肽的基因和产生多肽的方法。具有PDI活性的多肽的特征在于：A）具有催化蛋白质中二硫键交换的能力，B）主要以核糖核酸酶A为底物，C）具有20〜70℃的合适的活性温度，D）为在pH值为6〜9的条件下稳定，E）分子量约为60,000〜62,000。由于与常规PDI相比，具有较高的热稳定性并且在更宽的二硫苏糖醇浓度范围内表现出稳定的活性，因此可以提供可有利地用于某些蛋白质的重折叠反应的新型酶活性蛋白质。此外，还提供了使用Humicola insolens或用含有上述基因的表达载体转化的转化体能够有效地产生具有PDI活性的多肽的方法。

6. 发明授权

US09243042B2 Protein for constructing protein complex from Clostridium thermocellum, and use thereof 有权
标题翻译：用于从热纤维梭菌构建蛋白质复合物的蛋白质及其用途
公开(公告)号：US09243042B2
公开(公告)日：2016-01-26
申请号：US13072143
申请日：2011-03-25
申请人： Hiroaki Suzuki , Takao Imaeda , Katsunori Kohda
发明人： Hiroaki Suzuki , Takao Imaeda , Katsunori Kohda
IPC分类号： C07K14/33 , C12P7/10 , C12N9/24 , C12N9/42
CPC分类号： C07K14/33 , C12N9/2437 , C12N9/2482 , C12P7/10 , C12P2203/00 , C12Y302/01008 , Y02E50/16
摘要： It is an object to provide a protein having a dockerin, which is suited to production in yeasts and other eukaryotic microorganism in which sugar chain modification is predicted, and which provides excellent cohesin-dockerin binding ability, along with a use thereof. The present invention uses, as a protein for constructing a protein complex using a scaffolding protein having a type I cohesin from Clostridium thermocellum, a protein having a dockerin having at least one dockerin-specific sequence which is a dockerin-specific sequence associated with cohesin binding in type I dockerins from C. thermocellum, and which either has no intrinsic predicted N-type sugar chain modification site or has aspartic acid substituted for the asparagine of an intrinsic predicted N-type sugar chain modification site.
摘要翻译：本发明的目的是提供一种具有泊附蛋白的蛋白质，其适用于在酵母和其他预测糖链修饰的真核微生物中的生产，并且其具有优异的凝聚素 - 停泊聚蛋白结合能力及其用途。本发明使用具有来自嗜热链球菌（Clostridium thermocellum）的具有I型凝集素的支架蛋白构建蛋白质复合物的蛋白质，所述蛋白质具有具有至少一个停靠蛋白特异性序列的停泊聚糖蛋白，所述停靠蛋白特异性序列是与凝集素结合相关的停靠蛋白特异性序列来自C.therthercellum的I型码垛药，并且其没有内在预测的N型糖链修饰位点，或者天冬氨酸被本征预测的N型糖链修饰位点的天冬酰胺取代。

7. 发明申请

US20090035811A1 Artificial scaffolding material for protein retention and use of the same 有权
标题翻译：用于蛋白质保留和使用的人造脚手架材料
公开(公告)号：US20090035811A1
公开(公告)日：2009-02-05
申请号：US12219911
申请日：2008-07-30
申请人： Katsunori Kohda , Katsuhiro Ohno , Takao Imaeda , Kazuo Sakka
发明人： Katsunori Kohda , Katsuhiro Ohno , Takao Imaeda , Kazuo Sakka
IPC分类号： C12P1/02 , C12N11/16
CPC分类号： C12N11/16 , C07K14/33 , C12N9/2437 , C12N9/2445 , C12P7/10 , C12P7/40 , C12Y302/01004 , C12Y302/01021 , C12Y302/01091 , Y02E50/16
摘要： The present invention provides an artificial scaffolding material for retaining proteins suitable for placing contiguously one species or two or more species of proteins such as enzymes. To this end, the artificial scaffolding material for retaining proteins is provided with a cell and scaffolding proteins heterologous to the cell and placed on the surface layer side of the cell at an extent that allows aggregation properties to be conferred to the cell, and provided with a plurality of non-covalently binding protein-binding domains arranged in tandem.
摘要翻译：本发明提供了一种人造脚手架材料，其用于保留适于邻近地放置一种或两种或更多种蛋白质（例如酶）的蛋白质。为此，用于保留蛋白质的人造脚手架材料具有与细胞异源的细胞和支架蛋白，并以允许聚集性质赋予细胞的程度放置在细胞的表面层侧，并且具有多个非共价结合的蛋白质结合域串联布置。

8. 发明授权

US08361752B2 Artificial scaffolding material for protein retention and use of the same 有权
公开(公告)号：US08361752B2
公开(公告)日：2013-01-29
申请号：US12219911
申请日：2008-07-30
申请人： Katsunori Kohda , Katsuhiro Ohno , Takao Imaeda , Kazuo Sakka
发明人： Katsunori Kohda , Katsuhiro Ohno , Takao Imaeda , Kazuo Sakka
IPC分类号： C12P1/00 , C12P21/04 , C12N15/00
CPC分类号： C12N11/16 , C07K14/33 , C12N9/2437 , C12N9/2445 , C12P7/10 , C12P7/40 , C12Y302/01004 , C12Y302/01021 , C12Y302/01091 , Y02E50/16
摘要： The present invention provides an artificial scaffolding material for retaining proteins suitable for placing contiguously one species or two or more species of proteins such as enzymes. To this end, the artificial scaffolding material for retaining proteins is provided with a cell and scaffolding proteins heterologous to the cell and placed on the surface layer side of the cell at an extent that allows aggregation properties to be conferred to the cell, and provided with a plurality of non-covalently binding protein-binding domains arranged in tandem.

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