专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO1995020320A1 SELF-ASSEMBLING MULTIMERIC NUCLEIC ACID CONSTRUCTS 审中-公开
标题翻译：自组装多核核酸结构
公开(公告)号：WO1995020320A1
公开(公告)日：1995-08-03
申请号：PCT/US1995000864
申请日：1995-01-27
申请人： TRUSTEES OF BOSTON UNIVERSITY , UNIVERSITY OF MASSACHUSETTS MEDICAL CENTER
发明人： TRUSTEES OF BOSTON UNIVERSITY , UNIVERSITY OF MASSACHUSETTS MEDICAL CENTER , CANTOR, Charles, R. , NIEMEYER, Christof, M. , SMITH, Cassandra, L. , SANO, Takeshi , HNATOWICH, Donald, J. , RUSCKOWSKI, Mary
IPC分类号： A01N37/18
CPC分类号： B82Y5/00 , A61K47/55 , A61K47/557 , A61K47/6949 , A61K51/1268 , C03C17/30 , C03C17/3405 , C12Q1/6804 , C12Q1/682 , Y10S977/80 , Y10S977/808 , Y10S977/898 , Y10S977/906 , Y10S977/92
摘要： The invention is directed to constructs and compositions containing multimeric forms of nucleic acid. Multimeric nucleic acids comprise single-stranded nucleic acids attached via biotin to streptavidin and bound with a functional group. These constructs can be utilized in vivo to treat or identify diseased tissue or cells. Repeated administrations of multimeric nucleic acid compositions produce a rapid and specific amplification of nucleic acid constructs and their attached functional groups. For treatment purposes, functional groups may be toxins, radioisotopes, genes or enzymes. Diagnostically, labeled multimeric constructs may be used to identify specific targets in vivo or in vitro. Multimeric nucleic acids may also be used in nanotechnology and to create self-assembling polymeric aggregates such as membranes of defined porosity, microcircuits and many other products.
摘要翻译：本发明涉及包含核酸的多聚体形式的构建体和组合物。多聚核酸包含通过生物素与链霉亲和素连接并与官能团结合的单链核酸。这些构建体可以在体内用于治疗或鉴定患病组织或细胞。多重核酸组合物的重复施用产生核酸构建体及其连接的官能团的快速和特异性扩增。为了治疗目的，官能团可以是毒素，放射性同位素，基因或酶。在诊断上，标记的多聚体构建体可用于在体内或体外鉴定特异性靶标。多聚核酸也可用于纳米技术，并且可以产生自组装聚合物聚集体，例如确定的孔隙率的膜，微电路和许多其他产物。

2. 发明公开

EP0744894A1 SELF-ASSEMBLING MULTIMERIC NUCLEIC ACID CONSTRUCTS 失效
标题翻译：振作起来TRANSLATED多聚体结构NUCLEIC
公开(公告)号：EP0744894A1
公开(公告)日：1996-12-04
申请号：EP95909296.0
申请日：1995-01-27
申请人： TRUSTEES OF BOSTON UNIVERSITY , UNIVERSITY OF MASSACHUSETTS MEDICAL CENTER
发明人： CANTOR, Charles, R. , NIEMEYER, Christof, M. , SMITH, Cassandra, L. , SANO, Takeshi , HNATOWICH, Donald, J. , RUSCKOWSKI, Mary
IPC分类号： G01N33 , A61K38 , A61K39 , A61K45 , A61K47 , A61K49 , A61K51 , A61P31 , A61P35 , C03C17 , C07H21 , C12N15 , C12Q1
CPC分类号： B82Y5/00 , A61K47/55 , A61K47/557 , A61K47/6949 , A61K51/1268 , C03C17/30 , C03C17/3405 , C12Q1/6804 , C12Q1/682 , Y10S977/80 , Y10S977/808 , Y10S977/898 , Y10S977/906 , Y10S977/92
摘要： The invention is directed to constructs and compositions containing multimeric forms of nucleic acid. Multimeric nucleic acids comprise single-stranded nucleic acids attached via biotin to streptavidin and bound with a functional group. These constructs can be utilized in vivo to treat or identify diseased tissue or cells. Repeated administrations of multimeric nucleic acid compositions produce a rapid and specific amplification of nucleic acid constructs and their attached functional groups. For treatment purposes, functional groups may be toxins, radioisotopes, genes or enzymes. Diagnostically, labeled multimeric constructs may be used to identify specific targets in vivo or in vitro. Multimeric nucleic acids may also be used in nanotechnology and to create self-assembling polymeric aggregates such as membranes of defined porosity, microcircuits and many other products.

3. 发明申请

WO2005035725A2 METHODS FOR PRENATAL DIAGNOSIS OF CHROMOSOMAL ABNORMALITIES 审中-公开
标题翻译：用于临床诊断染色体异常的方法
公开(公告)号：WO2005035725A2
公开(公告)日：2005-04-21
申请号：PCT/US2004/033175
申请日：2004-10-08
申请人： THE TRUSTEES OF BOSTON UNIVERSITY , CANTOR, Charles, R. , DING, Chunming
发明人： CANTOR, Charles, R. , DING, Chunming
IPC分类号： C12N
CPC分类号： C12Q1/6883 , C12Q1/6806 , C12Q1/6827 , C12Q1/6876 , C12Q2600/154 , C12Q2600/156 , C12Q2600/166 , C12Q2521/331
摘要： Chromosomal abnormalities are responsible for a significant number of birth defects, including mental retardation. The present invention is related to methods for non-invasive and rapid, prenatal diagnosis of chromosomal abnormalities based on analysis of a maternal blood sample. The invention exploits the differences in DNA between the mother and fetus, for instance differences in their methylation states, as a means to enrich for fetal DNA in maternal plasma sample. The methods described herein can be used to detect chromosomal DNA deletions and duplications. In a preferred embodiment, the methods are used to diagnose chromosomal aneuploidy and related disorders, such as Down's and Turner's Syndrome.
摘要翻译：染色体异常负责大量的出生缺陷，包括精神发育迟滞。本发明涉及基于母体血液样品分析的非侵入性和快速，产前诊断染色体异常的方法。本发明利用母体和胎儿之间的DNA差异，例如甲基化状态的差异，作为富集母体血浆样品中胎儿DNA的手段。本文描述的方法可用于检测染色体DNA缺失和重复。在优选的实施方案中，所述方法用于诊断染色体非整倍体和相关疾病，例如唐氏和特纳综合征。

4. 发明申请

WO1997016699A1 PIEZOELECTRIC FORCE SENSING APPARATUS AND METHODS FOR BIOPOLYMER SEQUENCING 审中-公开
标题翻译：压电力传感装置和生物聚合物测序方法
公开(公告)号：WO1997016699A1
公开(公告)日：1997-05-09
申请号：PCT/US1996014462
申请日：1996-09-10
申请人： TRUSTEES OF BOSTON UNIVERSITY
发明人： TRUSTEES OF BOSTON UNIVERSITY , CANTOR, Charles, R. , SMITS, Johannes, G. , SMITH, Cassandra, L.
IPC分类号： G01B07/34
CPC分类号： C07K1/128 , B82Y35/00 , C12Q1/68 , C12Q1/6869 , C12Q2565/607
摘要： The invention relates to methods and apparatus for determining the structure of a biopolymer by piezoelectric force sensing. Biopolymers which can be analyzed include nucleic acids, proteins, polysaccharides and most any sequence of monomers. Biopolymers or biopolymer fragments are attached to a force sensing apparatus and subjected to an electric or magnetic field. The target generates a detectable force on the force sensing apparatus. Force information collected from multiple force sensing elements can be compiled and the structure of the biopolymer determined. Structure information determined may reveal sequence, size, mass or charge information specific for the biopolymer. The invention also relates to methods for the detection of biopolymers in a heterogenous mixture and methods for the detection of a disorder by piezoelectric force sensing. The invention further relates to methods and devices for measuring the mass of a molecular or macromolecular target by analyzing harmonic resonance frequencies.
摘要翻译：本发明涉及通过压电力感测来确定生物聚合物的结构的方法和装置。可以分析的生物聚合物包括核酸，蛋白质，多糖和大多数任何单体序列。生物聚合物或生物聚合物碎片连接到力感测装置并经受电场或磁场。目标在力感测装置上产生可检测力。可以编制从多个力传感元件收集的力信息，并确定生物聚合物的结构。确定的结构信息可能会显示生物聚合物特有的序列，大小，质量或电荷信息。本发明还涉及用于检测异种混合物中的生物聚合物的方法和通过压电力感测来检测病症的方法。本发明还涉及通过分析谐波谐振频率来测量分子或大分子靶的质量的方法和装置。

5. 发明申请

WO1996036731A2 NUCLEIC ACID DETECTION METHODS 审中-公开
标题翻译：核酸检测方法
公开(公告)号：WO1996036731A2
公开(公告)日：1996-11-21
申请号：PCT/US1996006527
申请日：1996-05-20
申请人： TRUSTEES OF BOSTON UNIVERSITY
发明人： TRUSTEES OF BOSTON UNIVERSITY , SMITH, Cassandra, L. , YAAR, Ron , SZAFRANSKI, Przemyslaw , CANTOR, Charles, R.
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6827 , C12Q1/6823 , C12Q2537/1376 , C12Q2521/307 , C12Q2535/131 , C12Q2525/151 , C12Q2565/501
摘要： The invention relates to methods for rapidly determining the sequence and/or length of a target sequence. The target sequence may be a series of known or unknown repeat sequences which are hybridized to an array of probes. The hybridized array is digested with a single-strand nuclease and free 3'-hydroxyl groups extended with a nucleic acid polymerase. Nuclease cleaved heteroduplexes can be easily distinguished from nuclease uncleaved heteroduplexes by differential labeling. Probes and target can be differentially labeled with detectable labels. Matched target can be detected by cleaving resulting loops from the hybridized target and creating free 3-hydroxyl groups. These groups are recognized and extended by polymerases added into the reaction system which also adds or releases one label into solution. Analysis of the resulting products using either solid phase or solution. These methods can be used to detect characteristic nucleic acid sequences, to determine target sequence and to screen for genetic defects and disorders. Assays can be conducted on solid surfaces allowing for multiple reactions to be conducted in parallel and, if desired, automated.
摘要翻译：本发明涉及用于快速确定靶序列的序列和/或长度的方法。靶序列可以是与探针阵列杂交的一系列已知或未知的重复序列。杂交阵列用单链核酸酶消化，游离的3'-羟基用核酸聚合酶延伸。核酸酶切割的异源双链体可以通过差异标记容易地与核酸酶未切割的异源双链体区分开。探针和靶可以用可检测的标记差异标记。可以通过从杂交靶标切割得到的环并产生游离的3-羟基来检测匹配的靶。这些基团被加入到反应体系中的聚合酶识别和扩增，该反应体系也将一个标记物添加或释放到溶液中。使用固相或溶液分析所得产物。这些方法可用于检测特征性核酸序列，确定靶序列并筛选遗传缺陷和病症。测定可以在固体表面进行，允许并行进行多个反应，如果需要，可以进行自动化。

6. 发明申请

WO2004033485A2 NUCLEIC ACID SUPPORTED PROTEIN COMPLEMENTATION 审中-公开
标题翻译：核酸支持的蛋白质补充
公开(公告)号：WO2004033485A2
公开(公告)日：2004-04-22
申请号：PCT/US2003/032426
申请日：2003-10-09
申请人： THE TRUSTEES OF BOSTON UNIVERSITY , CANTOR, Charles, R. , BROUDE, Natalia, E. , WITTE-HOFFMANN, Carlos
发明人： CANTOR, Charles, R. , BROUDE, Natalia, E. , WITTE-HOFFMANN, Carlos
IPC分类号： C07K
CPC分类号： C12Q1/6818 , C12Q1/6813 , C12Q2563/131 , C12Q2561/107
摘要： The present invention is directed to novel methods for in vitro and in vivo detection of target nucleic acid molecules, including DNA and RNA targets, as well as nucleic acid analogues. The present invention is based on protein complementation, in which two individual polypeptides are inactive. When the two inactive polypeptide fragment are brought in close proximity during hybridization to a target nucleic acid, they re-associate into an active, detectable protein.
摘要翻译：本发明涉及用于体外和体内检测靶核酸分子（包括DNA和RNA靶标）的新方法，如以及核酸类似物。本发明基于蛋白质互补，其中两个单独的多肽是无活性的。当两个非活性多肽片段在与靶核酸杂交期间非常接近时，它们重新结合成活性的可检测蛋白质。

7. 发明申请

WO2001096607A3 USE OF NUCLEOTIDE ANALOGS IN THE ANALYSIS OF OLIGONUCLEOTIDE MIXTURES AND IN HIGHLY MULTIPLEXED NUCLEIC ACID SEQUENCING 审中-公开
公开(公告)号：WO2001096607A3
公开(公告)日：2001-12-20
申请号：PCT/US2001/019249
申请日：2001-06-13
申请人： THE TRUSTEES OF BOSTON UNIVERSITY , CANTOR, Charles, R. , SIDDIQI, Fouad, A.
发明人： CANTOR, Charles, R. , SIDDIQI, Fouad, A.
IPC分类号： C12Q1/68
摘要： Methods and kits that use nucleotide analogs to confer increased accuracy and improved resolution in the analysis and sequencing of oligonucleotide mixtures are provided.

8. 发明申请

WO2007051002A2 ACTIVATED SPLIT-POLYPEPTIDES AND METHODS FOR THEIR PRODUCTION AND USE 审中-公开
标题翻译：活性分离多肽及其生产和使用方法
公开(公告)号：WO2007051002A2
公开(公告)日：2007-05-03
申请号：PCT/US2006/042299
申请日：2006-10-27
申请人： THE TRUSTEES OF BOSTON UNIVERSITY , BROUDE, Natalia , CANTOR, Charles, R. , DEMIDOV, Vadim, V.
发明人： BROUDE, Natalia , CANTOR, Charles, R. , DEMIDOV, Vadim, V.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883
摘要： The present invention relates to a method to produce activated split-polypeptide fragments that on reconstitution immediately forms an active protein. The method relate to real-time protein complementation. Also encompassed in the invention is a method to split and produce split-fluorescent proteins in an active state which produce a fluorescent signal immediately on reconstitution. The present application also provides methods to detect nucleic acids; non-nucleic acid analytes and nucleic acid hybridization in real-time using the novel activated split-polypeptide fragments of the invention.
摘要翻译：本发明涉及一种产生活化的裂多肽片段的方法，其在重构时立即形成活性蛋白质。该方法涉及实时蛋白质互补。本发明还包括分裂和产生活性状态的分裂荧光蛋白的方法，其在重建时立即产生荧光信号。本申请还提供了检测核酸的方法; 非核酸分析物和核酸杂交，使用本发明的新型活化裂解多肽片段。

9. 发明申请

WO2004065617A2 HAPLOTYPE ANALYSIS 审中-公开
标题翻译： HAPLOTYPE分析
公开(公告)号：WO2004065617A2
公开(公告)日：2004-08-05
申请号：PCT/US2004/001329
申请日：2004-01-16
申请人： THE TRUSTEES OF BOSTON UNIVERSITY , CANTOR, Charles, R. , DING, Chunming
发明人： CANTOR, Charles, R. , DING, Chunming
IPC分类号： C12Q
CPC分类号： C12Q1/6827 , C12Q1/6858 , C12Q2600/156 , C12Q2527/137 , C12Q2537/143 , C12Q2565/627
摘要： The present invention provides an efficient way for high throughput haplotype analysis. Several polymorphic nucleic acid markers, such as SNPs, can be simultaneously and reliably determined through multiplex PCR of single nucleic acid molecules in several parallel single molecule dilutions and the consequent statistical analysis of the results from these parallel single molecule multiplex PCR reactions results in reliable determination of haplotypes present in the subject. The nucleic acid markers can be of any distance to each other on the chromosome. In addition, an approach wherein overlapping DNA markers are analyzed can be used to link smaller haplotypes into larger haplotypes. Consequently, the invention provides a powerful new tool for diagnostic haplotyping and identifying novel haplotypes.
摘要翻译：本发明提供了高通量单倍型分析的有效方法。可以通过多个平行的单分子稀释物中的单个核酸分子的多重PCR来同时并可靠地测定几种多态性核酸标记，例如SNP，并且随后对来自这些平行的单分子多重PCR反应的结果的统计分析导致可靠的测定的单倍体存在于受试者。核酸标记在染色体上可以彼此有任何距离。此外，分析重叠的DNA标记物的方法可用于将较小的单倍型连接到较大的单元型中。因此，本发明为诊断单倍型和鉴定新型单体型提供了强大的新工具。

10. 发明申请

WO2004046321A2 CIS/TRANS RIBOREGULATORS 审中-公开
标题翻译： CIS / TRANS RIBOREGULATORS
公开(公告)号：WO2004046321A2
公开(公告)日：2004-06-03
申请号：PCT/US2003/036506
申请日：2003-11-14
申请人： TRUSTEES OF BOSTON UNIVERSITY , COLLINS, James, J. , ISAACS, Farren, J. , DWYER, Daniel, J. , CANTOR, Charles, R.
发明人： COLLINS, James, J. , ISAACS, Farren, J. , DWYER, Daniel, J. , CANTOR, Charles, R.
IPC分类号： C12N
CPC分类号： C12N15/67 , C12N15/11 , C12N2310/53 , C12Q1/6897
摘要： The present invention provides nucleic acid molecules, DNA constructs, plasmids, and methods for post-transcriptional regulation of gene expression using RNA molecules to both repress and activate translation of an open reading frame. Repression of gene expression is achieved through the presence of a regulatory nucleic acid element (the cis-repressive RNA or crRNA) within the 5' untranslated region (5' UTR) of an mRNA molecule. The nucleic acid element forms a hairpin (stem/loop) structure through complementary base pairing. The hairpin blocks access to the mRNA transcript by the ribosome, thereby preventing translation. In particular, in embodiments of the invention designed to operate in prokaryotic cells, the stem of the hairpin secondary structure sequesters the ribosome binding site (RBS). In embodiments of the invention designed to operate in eukaryotic cells, the stem of the hairpin is positioned upstream of the start codon, anywhere within the 5' UTR of an mRNA. A small RNA (trans-activating RNA, or taRNA), expressed in trans, interacts with the crRNA and alters the hairpin structure. This alteration allows the ribosome to gain access to the region of the transcript upstream of the start codon, thereby activating transcription from its previously repressed state.
摘要翻译：本发明提供核酸分子，DNA构建体，质粒和用于转录后调节基因表达的方法，其使用RNA分子抑制和激活开放阅读框的翻译。通过在mRNA分子的5'非翻译区（5'UTR）内存在调节性核酸元件（顺式抑制性RNA或crRNA）来实现基因表达的抑制。核酸元件通过互补碱基配对形成发夹（茎/环）结构。发夹阻止核糖体进入mRNA转录物，从而阻止翻译。特别地，在设计用于在原核细胞中操作的本发明的实施方案中，发夹二级结构的茎螯合核糖体结合位点（RBS）。在设计用于在真核细胞中操作的本发明的实施方案中，发夹的茎位于起始密码子的上游，位于mRNA的5'UTR内的任何位置。以反式表达的小RNA（反式激活RNA或者taRNA）与crRNA相互作用并改变发夹结构。这种改变允许核糖体进入起始密码子上游的转录物区域，从而从其先前的抑制状态激活转录。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式